Strain identifier

BacDive ID: 15074

Type strain: Yes

Species: Streptomyces celluloflavus

Strain history: KCC S-0126 <-- M. Mayama <-- M. Nishimura 39a.

NCBI tax ID(s): 58344 (species)

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General

@ref: 9840

BacDive-ID: 15074

DSM-Number: 40839

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic

description: Streptomyces celluloflavus DSM 40839 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 58344
  • Matching level: species

strain history

@refhistory
9840<- NRRL
67770KCC S-0126 <-- M. Mayama <-- M. Nishimura 39a.

doi: 10.13145/bacdive15074.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces celluloflavus
  • full scientific name: Streptomyces celluloflavus Nishimura et al. 1953 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Streptomyces kasugaensis

@ref: 9840

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces celluloflavus

full scientific name: Streptomyces celluloflavus Nishimura et al. 1953 emend. Nouioui et al. 2018

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
19491Brown10-14 daysISP 2
19491Beige10-14 daysISP 3
19491Yellow10-14 daysISP 4
19491Yellow10-14 daysISP 5
19491Yellow10-14 daysISP 6
19491Yellow10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19491yesAerial MyceliumWhiteISP 2
19491yesAerial MyceliumWhiteISP 3
19491yesAerial MyceliumWhiteISP 4
19491yesAerial MyceliumYellowISP 5
19491noISP 6
19491yesAerial MyceliumYellowISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9840GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19491ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19491ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19491ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19491ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19491ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19491ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperaturerange
19491positiveoptimum28mesophilic
9840positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 19491
  • spore description: Formation of spore chains (rectiflixibilis), spore surface smooth
  • type of spore: spore
  • spore formation: yes

compound production

@refcompound
9840aureothricin
9840thiolutin
20216aureothricin (=thiolutin)

halophily

  • @ref: 19491
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1949162968cellulose-
1949116634raffinose+/-
1949126546rhamnose+/-
1949128757fructose+
1949129864mannitol+
1949117268myo-inositol+/-
1949118222xylose+
1949117992sucrose+
1949122599arabinose+/-
1949117234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refmetaboliteproductionChebi-ID
67770aureothricinyes
67770thiolutinyes156450
68368acetoinyes15688
68368indoleno35581
68368hydrogen sulfideno16136

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19491----+-+--++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
9840soilJapanJPNAsia
67770Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
98401Risk group (German classification)
194911Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces celluloflavus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4126D44011121ena58344
20218Streptomyces celluloflavus 16S-23S ribosomal RNA gene spacer, partial sequenceU93345299ena58344
20218Streptomyces celluloflavus strain KCTC 9702 16S ribosomal RNA gene, partial sequenceAY9998291423ena58344
20218Streptomyces celluloflavus gene for 16S rRNA, partial sequence, strain: NBRC 13780AB1844761484ena58344

Genome sequences

  • @ref: 67770
  • description: Streptomyces celluloflavus NRRL B-2493
  • accession: GCA_000720995
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 58344

GC content

  • @ref: 67770
  • GC-content: 71.7
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno92.897no
flagellatedno98.08no
gram-positiveyes92.451no
anaerobicno99.1no
aerobicyes90.624no
halophileno89.41no
spore-formingyes94.48yes
glucose-utilyes89.354yes
thermophileno98.142yes
glucose-fermentno87.653no

External links

@ref: 9840

culture collection no.: DSM 40839, ATCC 29806, CECT 7364, ETH 24125, IFO 13780, KCC S-0126, NBRC 13780, NIHJ 198, NRRL B-2493, JCM 4126, CECT 3242, CGMCC 4.1659, KCTC 9702, NBRC 100597, RIA 779, VKM Ac-1819, VTT E-86258

straininfo link

  • @ref: 84180
  • straininfo: 39731

literature

  • topic: Phylogeny
  • Pubmed-ID: 23504246
  • title: Streptomyces araujoniae sp. nov.: an actinomycete isolated from a potato tubercle.
  • authors: da Silva LJ, Taketani RG, de Melo IS, Goodfellow M, Zucchi TD
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-013-9901-9
  • year: 2013
  • mesh: Antibiosis, Aspergillus/growth & development, Bacterial Typing Techniques, Base Sequence, DNA, Bacterial/genetics, Genes, rRNA, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology, Solanum tuberosum/*microbiology, Streptomyces/*classification/genetics/*isolation & purification
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9840Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40839)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40839
19491Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40839.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
84180Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID39731.1StrainInfo: A central database for resolving microbial strain identifiers