Strain identifier

BacDive ID: 15074

Type strain: Yes

Species: Streptomyces celluloflavus

Strain history: KCC S-0126 <-- M. Mayama <-- M. Nishimura 39a.

NCBI tax ID(s): 58344 (species)

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General

@ref: 9840

BacDive-ID: 15074

DSM-Number: 40839

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, Gram-positive

description: Streptomyces celluloflavus DSM 40839 is a spore-forming, Gram-positive bacterium that builds an aerial mycelium and was isolated from soil.

NCBI tax id

  • NCBI tax id: 58344
  • Matching level: species

strain history

@refhistory
9840<- NRRL
67770KCC S-0126 <-- M. Mayama <-- M. Nishimura 39a.

doi: 10.13145/bacdive15074.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces celluloflavus
  • full scientific name: Streptomyces celluloflavus Nishimura et al. 1953 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Streptomyces kasugaensis

@ref: 9840

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces celluloflavus

full scientific name: Streptomyces celluloflavus Nishimura et al. 1953 emend. Nouioui et al. 2018

type strain: yes

Morphology

cell morphology

@refgram stainconfidencemotility
69480positive90.765
6948090.088no

colony morphology

@refcolony colorincubation periodmedium used
19491Brown10-14 daysISP 2
19491Beige10-14 daysISP 3
19491Yellow10-14 daysISP 4
19491Yellow10-14 daysISP 5
19491Yellow10-14 daysISP 6
19491Yellow10-14 daysISP 7

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19491yesAerial MyceliumWhiteISP 2
19491yesAerial MyceliumWhiteISP 3
19491yesAerial MyceliumWhiteISP 4
19491yesAerial MyceliumYellowISP 5
19491noISP 6
19491yesAerial MyceliumYellowISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9840GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19491ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19491ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19491ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19491ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19491ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19491ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperature
19491positiveoptimum28
9840positivegrowth28
67770positivegrowth28

Physiology and metabolism

spore formation

  • @ref: 19491
  • spore description: Formation of spore chains (rectiflixibilis), spore surface smooth
  • type of spore: spore
  • spore formation: yes

compound production

@refcompound
9840aureothricin
9840thiolutin
20216aureothricin (=thiolutin)

halophily

  • @ref: 19491
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 2.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1949162968cellulose-
1949116634raffinose+/-
1949126546rhamnose+/-
1949128757fructose+
1949129864mannitol+
1949117268myo-inositol+/-
1949118222xylose+
1949117992sucrose+
1949122599arabinose+/-
1949117234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refmetaboliteproductionChebi-ID
67770aureothricinyes
67770thiolutinyes156450
68368acetoinyes15688
68368indoleno35581
68368hydrogen sulfideno16136

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19491----+-+--++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
9840soilJapanJPNAsia
67770Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
98401Risk group (German classification)
194911Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces celluloflavus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4126D44011121nuccore58344
20218Streptomyces celluloflavus 16S-23S ribosomal RNA gene spacer, partial sequenceU93345299nuccore58344
20218Streptomyces celluloflavus strain KCTC 9702 16S ribosomal RNA gene, partial sequenceAY9998291423nuccore58344
20218Streptomyces celluloflavus gene for 16S rRNA, partial sequence, strain: NBRC 13780AB1844761484nuccore58344

Genome sequences

  • @ref: 67770
  • description: Streptomyces celluloflavus NRRL B-2493
  • accession: GCA_000720995
  • assembly level: contig
  • database: ncbi
  • NCBI tax ID: 58344

GC content

  • @ref: 67770
  • GC-content: 71.7
  • method: genome sequence analysis

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingyes90.765no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no96.218no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes78.626no
69480spore-formingspore-formingAbility to form endo- or exosporesyes83.737yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95no
69480flagellatedmotile2+Ability to perform flagellated movementno90.088no

External links

@ref: 9840

culture collection no.: DSM 40839, ATCC 29806, CECT 7364, ETH 24125, IFO 13780, KCC S-0126, NBRC 13780, NIHJ 198, NRRL B-2493, JCM 4126, CECT 3242, CGMCC 4.1659, KCTC 9702, NBRC 100597, RIA 779, VKM Ac-1819, VTT E-86258

straininfo link

  • @ref: 84180
  • straininfo: 39731

literature

  • topic: Phylogeny
  • Pubmed-ID: 23504246
  • title: Streptomyces araujoniae sp. nov.: an actinomycete isolated from a potato tubercle.
  • authors: da Silva LJ, Taketani RG, de Melo IS, Goodfellow M, Zucchi TD
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-013-9901-9
  • year: 2013
  • mesh: Antibiosis, Aspergillus/growth & development, Bacterial Typing Techniques, Base Sequence, DNA, Bacterial/genetics, Genes, rRNA, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology, Solanum tuberosum/*microbiology, Streptomyces/*classification/genetics/*isolation & purification
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9840Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40839)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40839
19491Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40839.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
84180Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID39731.1StrainInfo: A central database for resolving microbial strain identifiers