Strain identifier

BacDive ID: 15042

Type strain: Yes

Species: Streptomyces azureus

Strain Designation: SC-2364

Strain history: KCC S-0564 <-- IFO 12744 <-- SAJ <-- ISP 5106 <-- IMRU 3705 <-- SC 2364.

NCBI tax ID(s): 146537 (species)

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General

@ref: 9317

BacDive-ID: 15042

DSM-Number: 40106

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic

description: Streptomyces azureus SC-2364 is a spore-forming, mesophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 146537
  • Matching level: species

strain history

@refhistory
9317<- E.B. Shirling, ISP <- W.H. Trejo, Squibb Inst. Med. Res., SC-2364
67770KCC S-0564 <-- IFO 12744 <-- SAJ <-- ISP 5106 <-- IMRU 3705 <-- SC 2364.

doi: 10.13145/bacdive15042.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces azureus
  • full scientific name: Streptomyces azureus Kelly et al. 1959 (Approved Lists 1980)

@ref: 9317

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces azureus

full scientific name: Streptomyces azureus Kelly et al. 1959 emend. Nouioui et al. 2018

strain designation: SC-2364

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no94.183
69480100positive

multimedia

  • @ref: 9317
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40106.jpg
  • caption: Medium 65 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9317GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
9317ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf

culture temp

@refgrowthtypetemperaturerange
18535positiveoptimum26mesophilic
9317positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

tolerance

  • @ref: 18535
  • compound: Lysozyme
  • percentage: 0.5

spore formation

@refspore formationconfidence
69481yes100
69480yes100

compound production

@refcompound
9317thiostrepton
20216Thiostrepton (bryamycin, thiactin)

halophily

  • @ref: 18535
  • salt: NaCl
  • growth: positive
  • tested relation: maximum
  • concentration: 5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1853517234glucose+
1853522599arabinose+
1853517992sucrose+/-
1853518222xylose+
1853517268myo-inositol+
1853529864mannitol+
1853528757fructose+
1853526546rhamnose+
1853516634raffinose+
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6777029693thiostreptonyes
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18535+++++-+--+-

Isolation, sampling and environmental information

isolation

  • @ref: 9317
  • sample type: soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_6264.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_91;97_97;98_1662;99_6264&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: EF178674
  • Sequence Identity:
  • Total samples: 166
  • soil counts: 112
  • aquatic counts: 2
  • animal counts: 49
  • plant counts: 3

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
93171Risk group (German classification)
185351German classification

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces cyaneus partial 16S rRNA gene, strain ISP 5106AJ3994701449ena1904
20218Streptomyces cyaneus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4564D44234121ena1904
20218Streptomyces azureus gene for 16S rRNA, partial sequence, strain: NBRC 12744AB1848371479ena146537
20218Streptomyces azureus strain NRRL B-2655 16S ribosomal RNA gene, partial sequenceEF1786741516ena146537

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces azureus strain ATCC 14921146537.3wgspatric146537
66792Streptomyces cyaneus strain ATCC 149211904.5plasmidpatric1904
66792Streptomyces azureus ATCC 149212775507102draftimg146537
67770Streptomyces azureus ATCC 14921GCA_001270025scaffoldncbi146537

GC content

@refGC-contentmethod
6777070.9genome sequence analysis
6777071.5thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno93.463no
gram-positiveyes88.235no
anaerobicno99.026no
aerobicyes94.042no
halophileno92.481no
spore-formingyes95.363no
thermophileno96.98yes
glucose-utilyes90.633no
flagellatedno97.23no
glucose-fermentno88.461no

External links

@ref: 9317

culture collection no.: DSM 40106, ATCC 14921, CBS 467.68, ETH 28555, IFO 12744, ISP 5106, JCM 4217, JCM 4564, NBRC 12744, RIA 1009, ATCC 19728, BCRC 12479, CGMCC 4.1675, IMET 43765, MTCC 3023, NBIMCC 1035, NRRL B-2655, PCM 2313, VKM Ac-719

straininfo link

  • @ref: 84148
  • straininfo: 35621

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny2983606A temperate phage of Streptomyces azureus.Ogata S, Suenaga H, Hayashida SAppl Environ Microbiol10.1128/aem.49.1.201-204.19851985Bacteriophages/growth & development/*isolation & purification/ultrastructure, Chromosome Mapping, DNA Restriction Enzymes/metabolism, DNA, Viral/analysis, Lysogeny, Microscopy, Electron, *Soil Microbiology, Species Specificity, Streptomyces, Virus ReplicationEnzymology
Metabolism10981703A chromosomal locus encoding a phosphoserine phosphatase- and a truncated MinD-like protein affects differentiation in Streptomyces azureus ATCC14921.Nishiyama T, Sakemi H, Sumi H, Tokunaga S, Doi K, Ogata SFEMS Microbiol Lett10.1111/j.1574-6968.2000.tb09275.x2000Adenosine Triphosphatases/*genetics/metabolism, Amino Acid Sequence, Base Sequence, *Escherichia coli Proteins, Molecular Sequence Data, Phosphoric Monoester Hydrolases/*genetics/metabolism, Plasmids/genetics, Sequence Analysis, DNA, Streptomyces/*enzymology/genetics/*physiology, Thiostrepton/metabolism, Transformation, Bacterial/genetics/physiologyGenetics
12784620Effects of UV dose on formation of spontaneously developing pocks in Streptomyces azureus ATCC14921.Yamada S, Suenaga H, Doi K, Yoshino S, Ogata SBiosci Biotechnol Biochem10.1271/bbb.67.7972003Dose-Response Relationship, Radiation, Microscopy, Electron, Plasmids/*radiation effects, Replicon/radiation effects, Spores, Bacterial, Streptomyces/genetics/*growth & development, *Ultraviolet Rays
16345806Specific Lysogenicity in Streptomyces azureus.Ogata S, Yoshino S, Suenaga H, Aoyama K, Kitajima N, Hayashida SAppl Environ Microbiol10.1128/aem.42.1.135-141.19811981
Biotechnology16346014Pock Formation of Streptomycetes endus with Production of Phage Taillike Particles.Ogata S, Suenaga H, Hayashida SAppl Environ Microbiol10.1128/aem.43.5.1182-1187.19821982
16348132Purine Auxotrophic Mutants with Altered Spore Color in Streptomyces azureus. ATCC 14921.Yamada S, Hayashida S, Ogata SAppl Environ Microbiol10.1128/aem.56.2.575-577.19901990
Genetics26494661Draft Genome Sequence of Thiostrepton-Producing Streptomyces azureus ATCC 14921.Sakihara K, Maeda J, Tashiro K, Fujino Y, Kuhara S, Ohshima T, Ogata S, Doi KGenome Announc10.1128/genomeA.01183-152015
Phylogeny29110155Streptomyces swartbergensis sp. nov., a novel tyrosinase and antibiotic producing actinobacterium.le Roes-Hill M, Prins A, Meyers PRAntonie Van Leeuwenhoek10.1007/s10482-017-0979-32017Anti-Bacterial Agents/*biosynthesis/isolation & purification/pharmacology, Bacteria/drug effects, DNA Gyrase/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Glycolipids/analysis, Melanins/analysis, Monophenol Monooxygenase/*biosynthesis, Peptidoglycan/analysis, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Soil Microbiology, South Africa, Streptomyces/chemistry/*classification/enzymology/genetics, Vitamin K 2/analysisEnzymology
Phylogeny33352827Characterization of a Novel Endophytic Actinomycete, Streptomyces physcomitrii sp. nov., and Its Biocontrol Potential Against Ralstonia solanacearum on Tomato.Zhuang X, Gao C, Peng C, Wang Z, Zhao J, Shen Y, Liu CMicroorganisms10.3390/microorganisms81220252020

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9317Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40106)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40106
18535Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40106.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
84148Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID35621.1StrainInfo: A central database for resolving microbial strain identifiers