Strain identifier

BacDive ID: 15001

Type strain: Yes

Species: Streptomyces arenae

Strain Designation: NA 269-M2

Strain history: KCC S-0452 <-- IFO 13016 <-- SAJ <-- ISP 5293 <-- Abbott Labs.; NA269-M2.

NCBI tax ID(s): 29301 (species)

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General

@ref: 9438

BacDive-ID: 15001

DSM-Number: 40293

keywords: 16S sequence, Bacteria, spore-forming, mesophilic, antibiotic compound production

description: Streptomyces arenae NA 269-M2 is a spore-forming, mesophilic bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 29301
  • Matching level: species

strain history

@refhistory
9438<- E.B. Shirling, ISP <- T.J. Oliver, Abbott Labs., NA 269-M2
67770KCC S-0452 <-- IFO 13016 <-- SAJ <-- ISP 5293 <-- Abbott Labs.; NA269-M2.

doi: 10.13145/bacdive15001.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces arenae
  • full scientific name: Streptomyces arenae Pridham et al. 1958 (Approved Lists 1980)

@ref: 9438

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces arenae

full scientific name: Streptomyces arenae Pridham et al. 1958

strain designation: NA 269-M2

type strain: yes

Morphology

colony morphology

@refincubation periodmedium used
1943310-14 daysISP 2
1943310-14 daysISP 3
1943310-14 daysISP 4
1943310-14 daysISP 5

multicellular morphology

@refforms multicellular complexcomplex namecomplex colormedium name
19433yesAerial MyceliumGreyISP 2
19433yesAerial MyceliumWhiteISP 3
19433yesAerial MyceliumWhiteISP 4
19433yesAerial MyceliumGreyISP 5

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9438GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19433ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19433ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19433ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19433ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
9438ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf

culture temp

@refgrowthtypetemperaturerange
19433positiveoptimum30mesophilic
9438positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

  • @ref: 19433
  • spore description: Formation of spore chains: spira, spore surface: spiny
  • spore formation: yes

compound production

@refcompound
9438mycomycetin
67770Mycomycetin

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1943362968cellulose+
1943316634raffinose+
1943326546rhamnose+
1943328757fructose+
1943329864mannitol+
1943317268myo-inositol+
1943318222xylose-
1943317992sucrose+
1943322599arabinose+
1943317234glucose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19433---------++

Isolation, sampling and environmental information

isolation

  • @ref: 9438
  • sample type: soil
  • geographic location: Illinois
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
94381Risk group (German classification)
194331Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces arenae partial 16S rRNA gene, strain ISP 5293AJ3994851447ena29301
20218Streptomyces arenae gene for 16S ribosomal RNA, partial sequence, strain: JCM 4452D44149121ena29301
20218Streptomyces arenae gene for 16S rRNA, partial sequence, strain: NBRC 13016AB2499771451ena29301

GC content

  • @ref: 67770
  • GC-content: 72.9
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 9438

culture collection no.: DSM 40293, ATCC 25428, CBS 926.69, ETH 24429, IFO 13016, ISP 5293, NBRC 13016, NRRL 2377, RIA 1208, JCM 4452, BCRC 11827, CGMCC 4.1610, VKM Ac-1201

straininfo link

  • @ref: 84109
  • straininfo: 38548

literature

  • topic: Pathogenicity
  • Pubmed-ID: 29457331
  • title: A strain of Streptomyces sp. isolated from rhizospheric soil of Crataegus oxycantha producing nalidixic acid, a synthetic antibiotic.
  • authors: Arora N, Kumar S, Satti NK, Ali A, Gupta P, Katoch M
  • journal: J Appl Microbiol
  • DOI: 10.1111/jam.13736
  • year: 2018
  • mesh: Anti-Bacterial Agents/*biosynthesis/chemistry/pharmacology, Cell Line, Transformed, Cell Survival/drug effects, Crataegus/*growth & development, Escherichia coli/drug effects, Humans, Nalidixic Acid/chemistry/*metabolism/pharmacology, Phylogeny, Plant Extracts, Pseudomonas aeruginosa/drug effects, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Streptomyces/classification/genetics/*isolation & purification/*metabolism
  • topic2: Phylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9438Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40293)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40293
19433Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40293.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
84109Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38548.1StrainInfo: A central database for resolving microbial strain identifiers