Strain identifier

BacDive ID: 14939

Type strain: Yes

Species: Kitasatospora albolonga

Strain Designation: 304 R7

Strain history: KCC S-0716 <-- IFO 13465 <-- SAJ <-- ISP 5570 <-- Bristol-Banyu Res. Inst.; 304R7.

NCBI tax ID(s): 68173 (species)

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General

@ref: 9651

BacDive-ID: 14939

DSM-Number: 40570

keywords: 16S sequence, Bacteria, antibiotic compound production

description: Kitasatospora albolonga 304 R7 is a bacterium that builds an aerial mycelium and produces antibiotic compounds.

NCBI tax id

  • NCBI tax id: 68173
  • Matching level: species

strain history

@refhistory
9651<- E.B. Shirling, ISP <- H. Tsukiura, 304 R7
67770KCC S-0716 <-- IFO 13465 <-- SAJ <-- ISP 5570 <-- Bristol-Banyu Res. Inst.; 304R7.

doi: 10.13145/bacdive14939.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Kitasatospora
  • species: Kitasatospora albolonga
  • full scientific name: Kitasatospora albolonga (Tsukiura et al. 1964) Labeda et al. 2017

  • synonyms

    • @ref: 20215
    • synonym: Streptomyces albolongus

@ref: 9651

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Kitasatospora

species: Kitasatospora albolonga

full scientific name: Kitasatospora albolonga (Tsukiura et al. 1964) Labeda et al. 2017

strain designation: 304 R7

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
18659Beige (1001)10-14 daysISP 2
18659Beige (1001)10-14 daysISP 3
18659Beige (1001)10-14 daysISP 4
18659Beige (1001)10-14 daysISP 5
18659Terra brown (8028)10-14 daysISP 6
18659Beige (1001)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium namecomplex namecomplex color
18659noISP 2
18659yesISP 3Aerial MyceliumCream
18659yesISP 4Aerial MyceliumCream
18659noISP 5
18659noISP 6
18659noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9651GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18659ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18659ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18659ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18659ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18659ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18659ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
9651ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf

culture temp

@refgrowthtypetemperature
18659positiveoptimum28
9651positivegrowth28
67770positivegrowth28

Physiology and metabolism

compound production

@refcompound
9651proceomycin
18659Ansathiazin
18659Awamycin
67770Proceomycin

observation

  • @ref: 67770
  • observation: quinones: MK-9(H8), MK-9(H6)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1865917234glucose+
1865922599arabinose+
1865917992sucrose-
1865918222xylose-
1865917268myo-inositol-
1865929864mannitol-
1865928757fructose-
1865926546rhamnose-
1865916634raffinose-
1865962968cellulose+
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6836815688acetoin+
6836835581indole-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-mannosidase+3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18659+++----+-++++-++++-

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18659+++++-+--++

Isolation, sampling and environmental information

isolation

  • @ref: 67770
  • sample type: Soil
  • geographic location: suburbs of Fukuyama City, Hiroshima Pref.
  • country: Japan
  • origin.country: JPN
  • continent: Asia

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
96511Risk group (German classification)
186591German classification

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Streptomyces albolongus strain DSM 40570 16S ribosomal RNA gene, partial sequenceAY6866531292nuccore68173
20218Streptomyces albolongus strain JCM 4716 16S ribosomal RNA gene, partial sequenceAY9997561422nuccore68173
20218Streptomyces albolongus gene for 16S ribosomal RNA, partial sequence, strain: JCM 4716D44306120nuccore68173
20218Kitasatospora albolonga gene for 16S rRNA, partial sequence, strain: NBRC 13465AB1844251476nuccore68173
9651Kitasatospora albolonga strain DSM 40570 16S ribosomal RNA, partial sequenceNR_1153091292nuccore68173

External links

@ref: 9651

culture collection no.: DSM 40570, ATCC 27414, CBS 766.72, IFO 13465, ISP 5570, JCM 4716, NBRC 13465, RIA 1426, BCRC 15179, CGMCC 4.1661, KCTC 9676, KCTC 9749, NRRL B-3604, NRRL ISP-5570, VKM Ac-704

straininfo link

  • @ref: 84048
  • straininfo: 44758

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny23504246Streptomyces araujoniae sp. nov.: an actinomycete isolated from a potato tubercle.da Silva LJ, Taketani RG, de Melo IS, Goodfellow M, Zucchi TDAntonie Van Leeuwenhoek10.1007/s10482-013-9901-92013Antibiosis, Aspergillus/growth & development, Bacterial Typing Techniques, Base Sequence, DNA, Bacterial/genetics, Genes, rRNA, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil Microbiology, Solanum tuberosum/*microbiology, Streptomyces/*classification/genetics/*isolation & purificationGenetics
Enzymology29739601Cloning, characterization and substrate degradation mode of a novel chitinase from Streptomyces albolongus ATCC 27414.Gao L, Sun J, Secundo F, Gao X, Xue C, Mao XFood Chem10.1016/j.foodchem.2018.04.0682018Bacterial Proteins/*chemistry/*genetics/metabolism, Chitin/metabolism, Chitinases/*chemistry/*genetics/metabolism, *Cloning, Molecular, Enzyme Stability, Hydrogen-Ion Concentration, Hydrolysis, Molecular Weight, Recombinant Proteins/metabolism, Streptomyces/chemistry/*enzymology/genetics, Substrate Specificity, TemperatureMetabolism
30824097Efficient enzymatic hydrolysis of ionic liquid pretreated chitin and its dissolution mechanism.Li J, Huang WC, Gao L, Sun J, Liu Z, Mao XCarbohydr Polym10.1016/j.carbpol.2019.02.0272019
Enzymology30827665Cloning, expression and characterization of a novel chitosanase from Streptomyces albolongus ATCC 27414.Guo N, Sun J, Wang W, Gao L, Liu J, Liu Z, Xue C, Mao XFood Chem10.1016/j.foodchem.2019.02.0562019Bacterial Proteins/genetics/metabolism, Chitin/analogs & derivatives/metabolism, Chitosan/metabolism, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Enzyme Stability, Escherichia coli/genetics, Glucosamine/metabolism, Glycoside Hydrolases/*genetics/*metabolism, Hydrogen-Ion Concentration, Hydrolysis, Molecular Weight, Oligosaccharides, Recombinant Proteins/genetics/metabolism, Streptomyces/*enzymology/geneticsMetabolism
Phylogeny31188092Streptomyces rhizosphaericola sp. nov., an actinobacterium isolated from the wheat rhizosphere.Vargas Hoyos HA, Nobre Santos S, Da Silva LJ, Paulino Silva FS, Bonaldo Genuario D, Domingues Zucchi T, Melo ISInt J Syst Evol Microbiol10.1099/ijsem.0.0034982019Bacterial Typing Techniques, Base Composition, Brazil, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Multilocus Sequence Typing, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/isolation & purification, Triticum/*microbiologyTranscriptome
32478051A Novel Soluble Squalene-Hopene Cyclase and Its Application in Efficient Synthesis of Hopene.Liu Z, Zhang Y, Sun J, Huang WC, Xue C, Mao XFront Bioeng Biotechnol10.3389/fbioe.2020.004262020

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9651Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40570)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40570
18659Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40570.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68382Automatically annotated from API zym
84048Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID44758.1StrainInfo: A central database for resolving microbial strain identifiers