Strain identifier

BacDive ID: 14648

Type strain: Yes

Species: Mammaliicoccus fleurettii

Strain Designation: 241

Strain history: CIP <- 1999, C. Vernozy Rozand, Lyon Nat. Vet. School, Lyon, France: strain 241

NCBI tax ID(s): 150056 (species)

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General

@ref: 4966

BacDive-ID: 14648

DSM-Number: 13212

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped

description: Mammaliicoccus fleurettii 241 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from goat milk cheese.

NCBI tax id

  • NCBI tax id: 150056
  • Matching level: species

strain history

@refhistory
4966<- C. Vernozy-Rozand; 241
119394CIP <- 1999, C. Vernozy Rozand, Lyon Nat. Vet. School, Lyon, France: strain 241

doi: 10.13145/bacdive14648.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Mammaliicoccus
  • species: Mammaliicoccus fleurettii
  • full scientific name: Mammaliicoccus fleurettii (Vernozy-Rozand et al. 2000) Madhaiyan et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Staphylococcus fleurettii

@ref: 4966

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Staphylococcaceae

genus: Mammaliicoccus

species: Mammaliicoccus fleurettii

full scientific name: Mammaliicoccus fleurettii (Vernozy-Rozand et al. 2000) Madhaiyan et al. 2020

strain designation: 241

type strain: yes

Morphology

cell morphology

  • @ref: 119394
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@reftype of hemolysis
4966gamma
119394

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4966COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
4966TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
42179MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
119394CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29

culture temp

@refgrowthtypetemperaturerange
4966positivegrowth37mesophilic
42179positivegrowth30mesophilic
56052positivegrowth30-37mesophilic
119394positivegrowth10-41
119394nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
56052aerobe
119394obligate aerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371esculin+builds acid from4853
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol+builds acid from17754
119394hippurate+hydrolysis606565
119394nitrate+reduction17632
119394nitrite-reduction16301
68375urea-hydrolysis16199
68375arginine-hydrolysis29016
68375ornithine-degradation18257
68375esculin-hydrolysis4853
68375D-glucose+fermentation17634
68375D-fructose+fermentation15824
68375maltose-fermentation17306
68375lactose-fermentation17716
68375trehalose-fermentation27082
68375D-mannitol-fermentation16899
68375raffinose-fermentation16634
68375D-ribose-fermentation16988
68375cellobiose-fermentation17057
68375nitrate+reduction17632
68375sucrose-fermentation17992
68375N-acetylglucosamine-fermentation59640
68375turanose-fermentation32528
68375arabinose-fermentation22599

metabolite production

  • @ref: 119394
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 119394
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68375beta-glucuronidase-3.2.1.31
68375pyrrolidonyl arylamidase-3.4.19.3
68375alkaline phosphatase-3.1.3.1
68375L-arginine arylamidase-
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase-3.5.3.6
68375urease-3.5.1.5
119394oxidase+
119394beta-galactosidase-3.2.1.23
119394alcohol dehydrogenase-1.1.1.1
119394gelatinase+/-
119394amylase-
119394DNase-
119394caseinase-3.4.21.50
119394catalase+1.11.1.6
119394coagulase-
119394tween esterase-
119394gamma-glutamyltransferase-2.3.2.2
119394lecithinase-
119394lipase-
119394lysine decarboxylase-4.1.1.18
119394ornithine decarboxylase-4.1.1.17
119394phenylalanine ammonia-lyase-4.3.1.24
119394protease-
119394urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    56052C14:0114
    56052C16:01.716
    56052C13:0 iso1.412.612
    56052C14:0 ISO5.313.618
    56052C15:0 ANTEISO36.314.711
    56052C15:0 ISO35.714.621
    56052C16:0 iso3.615.626
    56052C16:1 ω11c0.615.757
    56052C16:1 ω7c alcohol1.215.386
    56052C17:0 anteiso5.216.722
    56052C17:0 iso3.816.629
    56052C17:1 ω10c ISO1.916.387
    56052C18:2 ω6,9c/C18:0 ANTE1.117.724
    56052unknown 15.6651.215.665
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119394-+++-+-+--++---++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119394+--+++----+++----+-----+/-++/-+/-+--++------++-------+/--

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
4966----+++-------++----+-----
4966----++--------+-----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
4966goat milk cheeseFrance, LyonFranceFRAEurope
56052Cheese made from raw goat's milk
119394Food, Goat, raw milk cheeseLyonFranceFRAEurope1995

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Dairy product
#Host#Mammals#Caprinae (Sheep/Goat)
#Host Body Product#Fluids#Milk

taxonmaps

  • @ref: 69479
  • File name: preview.99_4196.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3;97_96;98_105;99_4196&stattab=map
  • Last taxonomy: Staphylococcus
  • 16S sequence: AB233330
  • Sequence Identity:
  • Total samples: 998
  • soil counts: 25
  • aquatic counts: 30
  • animal counts: 892
  • plant counts: 51

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
49661Risk group (German classification)
1193941Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus fleurettii strain DSM 13212 16S ribosomal RNA gene, partial sequenceAY688058465ena150056
4966Staphylococcus fleurettii gene for 16S rRNA, partial sequence, strain: GTC 1999AB2333301466ena150056

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mammaliicoccus fleurettii DSM 13212GCA_003970575scaffoldncbi150056
66792Staphylococcus fleurettii strain DSM 13212150056.28wgspatric150056

GC content

  • @ref: 4966
  • GC-content: 31
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno87.389no
flagellatedno91.162no
gram-positiveyes93.13no
anaerobicno99.788yes
aerobicyes96.222yes
halophileyes93.886no
spore-formingno87.701no
thermophileno99.755yes
glucose-utilyes86.7no
glucose-fermentyes78.522yes

External links

@ref: 4966

culture collection no.: DSM 13212, ATCC BAA 274, CIP 106114, CCUG 43834

straininfo link

  • @ref: 83776
  • straininfo: 44342

literature

  • topic: Phylogeny
  • Pubmed-ID: 10939659
  • title: Staphylococcus fleurettii sp. nov., isolated from goat's milk cheeses.
  • authors: Vernozy-Rozand C, Mazuy C, Meugnier H, Bes M, Lasne Y, Fiedler F, Etienne J, Freney J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-50-4-1521
  • year: 2000
  • mesh: Animals, Anti-Bacterial Agents/pharmacology, Cell Wall/chemistry, Cheese/*microbiology, DNA, Bacterial/genetics, DNA, Ribosomal/chemistry, Drug Resistance, Microbial, *Food Microbiology, Goats, Novobiocin/pharmacology, Nucleic Acid Hybridization, Phenotype, Polymerase Chain Reaction, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Ribotyping, Staphylococcus/drug effects/*isolation & purification/physiology
  • topic2: Biotechnology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4966Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13212)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13212
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42179Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18209
56052Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 43834)https://www.ccug.se/strain?id=43834
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83776Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID44342.1StrainInfo: A central database for resolving microbial strain identifiers
119394Curators of the CIPCollection of Institut Pasteur (CIP 106114)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106114