Staphylococcus fleurettii (DSM 13212, ATCC BAA-274, CIP 106114)

Name: Staphylococcus fleurettii (DSM 13212, ATCC BAA-274, CIP 106114)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 14648

Type strain

Strain Designation 241

Culture col. no. DSM 13212 ATCC BAA-274 CIP 106114 CCUG 43834 241

NCBI tax ID(s) 1255628 150056

Special Collections DSMZ CCUG CIP

Links

Staphylococcus fleurettii 241 is an aerobe, mesophilic, Gram-positive prokaryote that was isolated from goat milk cheese.

Gram-positive coccus-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 44342

ATCC BAA-274,DSM 13212,CIP 106114,CCUG 43834,CCM 4922,NCIMB 14041

@ref 20215

Last LPSN update 2025-12-16 09:50:24

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Staphylococcaceae

Genus Staphylococcus

Species Staphylococcus fleurettii

Full scientific name Staphylococcus fleurettii Vernozy-Rozand et al. 2000

Synonyms (1)

Mammaliicoccus fleurettii

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
119394	positive	coccus-shaped

Colony morphology

@ref	Type of hemolysis
4966	gamma
119394

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
4966	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
4966	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
42179	MEDIUM 29- Brain heart agar		Distilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
119394	CIP Medium 29	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
4966	positive	growth	37	mesophilic
42179	positive	growth	30	mesophilic
56052	positive	growth	30-37	mesophilic
119394	positive	growth	10-41
119394	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
56052	aerobe
119394	obligate aerobe
125439	obligate aerobe	96.6

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68375	22599 ChEBI	arabinose	-	fermentation	from API ID32STA
68375	29016 ChEBI	arginine	-	hydrolysis	from API ID32STA
68375	17057 ChEBI	cellobiose	-	fermentation	from API ID32STA
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68375	15824 ChEBI	D-fructose	+	fermentation	from API ID32STA
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68375	17634 ChEBI	D-glucose	+	fermentation	from API ID32STA
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68375	16899 ChEBI	D-mannitol	-	fermentation	from API ID32STA
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68375	16988 ChEBI	D-ribose	-	fermentation	from API ID32STA
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68375	4853 ChEBI	esculin	-	hydrolysis	from API ID32STA
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	+	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
119394	606565 ChEBI	hippurate	+	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68375	17716 ChEBI	lactose	-	fermentation	from API ID32STA
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68375	17306 ChEBI	maltose	-	fermentation	from API ID32STA
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68375	59640 ChEBI	N-acetylglucosamine	-	fermentation	from API ID32STA
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
68375	17632 ChEBI	nitrate	+	reduction	from API ID32STA
119394	17632 ChEBI	nitrate	+	reduction
119394	16301 ChEBI	nitrite	-	reduction
68375	18257 ChEBI	ornithine	-	degradation	from API ID32STA
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68375	16634 ChEBI	raffinose	-	fermentation	from API ID32STA
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68375	17992 ChEBI	sucrose	-	fermentation	from API ID32STA
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68375	27082 ChEBI	trehalose	-	fermentation	from API ID32STA
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68375	32528 ChEBI	turanose	-	fermentation	from API ID32STA
68371	32528 ChEBI	turanose	+	builds acid from	from API 50CH acid
68375	16199 ChEBI	urea	-	hydrolysis	from API ID32STA
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
119394	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
119394	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
119394	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68375	alkaline phosphatase	-	3.1.3.1	from API ID32STA
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
119394	amylase	-
68375	arginine dihydrolase	-	3.5.3.6	from API ID32STA
68382	beta-galactosidase	-	3.2.1.23	from API zym
119394	beta-galactosidase	-	3.2.1.23
68375	beta-galactosidase	-	3.2.1.23	from API ID32STA
68382	beta-glucosidase	+	3.2.1.21	from API zym
68375	beta-glucosidase	-	3.2.1.21	from API ID32STA
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68375	beta-glucuronidase	-	3.2.1.31	from API ID32STA
119394	caseinase	-	3.4.21.50
119394	catalase	+	1.11.1.6
119394	coagulase	-
68382	cystine arylamidase	+	3.4.11.3	from API zym
119394	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
119394	gamma-glutamyltransferase	-	2.3.2.2
119394	gelatinase	+/-
68375	L-arginine arylamidase	-		from API ID32STA
119394	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
119394	lipase	-
68382	lipase (C 14)	-		from API zym
119394	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119394	ornithine decarboxylase	-	4.1.1.17
68375	ornithine decarboxylase	-	4.1.1.17	from API ID32STA
119394	oxidase	+
119394	phenylalanine ammonia-lyase	-	4.3.1.24
119394	protease	-
68375	pyrrolidonyl arylamidase	-	3.4.19.3	from API ID32STA
68382	trypsin	-	3.4.21.4	from API zym
119394	tween esterase	-
119394	urease	-	3.5.1.5
68375	urease	-	3.5.1.5	from API ID32STA
68382	valine arylamidase	-		from API zym

Fatty acid profile

Metadata FA analysis

@ref

56052

Fatty acid	Percentage	ECL
C15:0 ANTEISO	36.3	14.711
C15:0 ISO	35.7	14.621
C14:0 ISO	5.3	13.618
C17:0 anteiso	5.2	16.722
C17:0 iso	3.8	16.629
C16:0 iso	3.6	15.626
C17:1 ω10c ISO	1.9	16.387
C16:0	1.7	16
C13:0 iso	1.4	12.612
C16:1 ω7c alcohol	1.2	15.386
unknown 15.665	1.2	15.665
C18:2 ω6,9c/C18:0 ANTE	1.1	17.724
C14:0	1	14
C16:1 ω11c	0.6	15.757

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119394	-	+	+	+	-	+	-	+	-	-	+	+	-	-	-	+	+	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
119394	not determinedn.d.	+	-	-	+	+	+	-	-	-	-	+	+	+	-	-	-	-	+	-	-	-	-	-	+/-	+	+/-	+/-	+	-	-	+	+	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	+/-	-

API ID32STA

@ref	URE	ADH (Arg)	ODC	ESC	GLU	FRU	MNE	MAL	LAC	TRE	MAN	RAF	RIB	CEL	Reduction of nitrateNIT	Acetoin production (Voges Proskauer test)VP	beta GAL	L-arginine arylamidaseArgA	PAL	Pyrrolidonyl arylamidasePyrA	NOVO	SAC	NAG	TUR	ARA	beta GUR
4966	-	-	-	-	+	+	+	-	-	-	-	-	-	-	+	+	-	-	-	-	+	-	-	-	-	-
4966	-	-	-	-	+	+	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Dairy product
#Host	#Mammals	#Caprinae (Sheep/Goat)
#Host Body Product	#Fluids	#Milk

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
4966	goat milk cheese	France, Lyon	France	FRA	Europe
56052	Cheese made from raw goat's milk
119394	Food, Goat, raw milk cheese	Lyon	France	FRA	Europe	1995

Taxonmaps

69479

Global distribution of 16S sequence AB233330 (>99% sequence identity) for Staphylococcus from Microbeatlas

Aquatic Samples	30
Soil Samples	25
Animal Samples	892
Plant Samples	51
Total Samples	998

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4966	1	Risk group (German classification) According to TRBA 466
119394	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
66792	ASM397057v1 assembly for Mammaliicoccus fleurettii DSM 13212	scaffold	GCA_003970575	45972.57	150056	28.06

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Staphylococcus fleurettii strain DSM 13212 16S ribosomal RNA gene, partial sequence	AY688058	465	150056
4966	Staphylococcus fleurettii gene for 16S rRNA, partial sequence, strain: GTC 1999	AB233330	1466	150056
124043	Staphylococcus fleurettii strain DSM 13212 16S ribosomal RNA gene, partial sequence 16S-23S ribosomal RNA intergenic spacer, complete sequence and 23S ribosomal RNA gene, partial sequence.	MF678880	4300	150056
124043	Staphylococcus fleurettii strain DSM 13212 16S ribosomal RNA gene, partial sequence.	MF678936	1284	150056

GC content

@ref	GC-content (mol%)	Method
4966	31	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Mammaliicoccus fleurettii DSM 13212
NCBI: GCA_003970575

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	57.20	no
125439	motility	BacteriaNetⓘ	yes	51.30	no
125439	gram_stain	BacteriaNetⓘ	positive	73.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Mammaliicoccus fleurettii DSM 13212
NCBI: GCA_003970575.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	85.00	no
125438	anaerobic	anaerobicⓘ	no	98.40	no
125438	aerobic	aerobicⓘ	yes	75.99	yes
125438	spore-forming	spore-formingⓘ	no	62.65	no
125438	thermophilic	thermophileⓘ	no	93.58	yes
125438	flagellated	motile2+ⓘ	no	77.88	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Iron-based microbial interactions: the role of iron metabolism in the cheese ecosystem.	Mekuli R, Shoukat M, Dugat-Bony E, Bonnarme P, Landaud S, Swennen D, Herve V.	J Bacteriol	10.1128/jb.00539-24	2025
Phylogeny	Staphylococcus fleurettii sp. nov., isolated from goat's milk cheeses.	Vernozy-Rozand C, Mazuy C, Meugnier H, Bes M, Lasne Y, Fiedler F, Etienne J, Freney J	Int J Syst Evol Microbiol	10.1099/00207713-50-4-1521	2000

References

#4966	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13212
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#42179	; Curators of the CIP;
#56052	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 43834
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68371	Automatically annotated from API 50CH acid .
#68375	Automatically annotated from API ID32STA .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119394	Collection of Institut Pasteur ; Curators of the CIP; CIP 106114
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Staphylococcus fleurettii (DSM 13212, ATCC BAA-274, CIP 106114)

Name and taxonomic classification