Strain identifier

BacDive ID: 14587

Type strain: Yes

Species: Staphylococcus warneri

Strain Designation: AW 25

Strain history: CIP <- 1981, J. Pillet, Inst. Pasteur, Paris, France <- K.H. Schleifer, Germany

NCBI tax ID(s): 1292 (species)

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General

@ref: 8594

BacDive-ID: 14587

DSM-Number: 20316

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped, human pathogen

description: Staphylococcus warneri AW 25 is an aerobe, mesophilic, Gram-positive human pathogen that was isolated from human skin.

NCBI tax id

  • NCBI tax id: 1292
  • Matching level: species

strain history

@refhistory
8594<- K.H. Schleifer <- W.E. Kloos, AW 25
67770Y. Kosako <-- R. Sakazaki <-- ATCC 27836 <-- W. E. Kloos AW 25.
123912CIP <- 1981, J. Pillet, Inst. Pasteur, Paris, France <- K.H. Schleifer, Germany

doi: 10.13145/bacdive14587.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus warneri
  • full scientific name: Staphylococcus warneri Kloos and Schleifer 1975 (Approved Lists 1980)

@ref: 8594

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus warneri

full scientific name: Staphylococcus warneri Kloos and Schleifer 1975

strain designation: AW 25

type strain: yes

Morphology

cell morphology

  • @ref: 123912
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@refincubation periodhemolysis ability
85941-2 days
1239121

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8594COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8594TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
39706MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
123912CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
123912CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
8594positivegrowth37mesophilic
39706positivegrowth37mesophilic
44976positivegrowth37mesophilic
67770positivegrowth37mesophilic
123912positivegrowth22-45
123912nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
44976aerobe
123912facultative anaerobe

murein

  • @ref: 8594
  • murein short key: A11.03
  • type: A3alpha L-Lys-Gly2-4-L-Ser1-2-Gly

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
123912606565hippurate-hydrolysis
12391217632nitrate-reduction
12391216301nitrite-reduction
6837516199urea+hydrolysis
6837518257ornithine-degradation
683754853esculin-hydrolysis
6837515824D-fructose+fermentation
6837516024D-mannose-fermentation
6837517306maltose+fermentation
6837517716lactose-fermentation
6837527082trehalose+fermentation
6837516899D-mannitol+fermentation
6837516634raffinose-fermentation
6837516988D-ribose-fermentation
6837517057cellobiose-fermentation
6837517632nitrate-reduction
6837517992sucrose+fermentation
6837559640N-acetylglucosamine-fermentation
6837532528turanose-fermentation
6837522599arabinose-fermentation

metabolite production

  • @ref: 123912
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 123912
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
8594catalase+1.11.1.6
8594cytochrome-c oxidase-1.9.3.1
68375beta-glucuronidase+3.2.1.31
68375pyrrolidonyl arylamidase-3.4.19.3
68375L-arginine arylamidase-
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375urease+3.5.1.5
123912oxidase-
123912beta-galactosidase-3.2.1.23
123912alcohol dehydrogenase+1.1.1.1
123912gelatinase-
123912amylase-
123912DNase-
123912caseinase-3.4.21.50
123912catalase+1.11.1.6
123912coagulase-
123912tween esterase-
123912lecithinase-
123912lipase-
123912lysine decarboxylase-4.1.1.18
123912phenylalanine ammonia-lyase-4.3.1.24
123912protease-
123912urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123912-+++-+----++--++----

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
8594++--++-+-++----+--+/--+/-+---+
8594++--++-+-++----------+---+
8594+---++-+-++----+----++---+
8594++--++-+-++----+----++---+
8594++--++-+-++----+-----+---+
8594++--++-+-++----+--+/--++---+
8594++---+-+-++----+----++---+
8594++--++-+-++----+----++---+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
8594human skinUSAUSANorth America
44976Human skin
67770Human skin
123912Human, Skin

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Organ#Skin, Nail, Hair

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
8594yes, in single casesyes, in single cases1Risk group (German classification)
1239121Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus warneri strain DSM 20316 16S ribosomal RNA gene, partial sequenceAY688106464ena1292
20218S.warneri gene for 16S ribosomal RNAZ26903771ena1292
8594Staphylococcus warneri 16S ribosomal RNA (16S rRNA) geneL376031470ena1292

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Staphylococcus warneri NCTC11044GCA_900636385completencbi1292
66792Staphylococcus warneri L37603596319.3wgspatric596319
66792Staphylococcus warneri strain NCTC 110441292.58wgspatric1292
66792Staphylococcus warneri strain NCTC110441292.103completepatric1292
67770Staphylococcus warneri NCTC 11044GCA_002901765scaffoldncbi1292

GC content

@refGC-contentmethod
859434.2
6777034.2thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno93.974no
gram-positiveyes90.512no
anaerobicno99.313yes
aerobicyes91.145no
halophileyes96.234no
spore-formingno92.726no
thermophileno99.755no
glucose-utilyes90.85no
motileno91.032no
glucose-fermentyes82.655no

External links

@ref: 8594

culture collection no.: DSM 20316, ATCC 27836, CCM 2730, CCUG 7325, NCTC 11044, JCM 2415, BCRC 12929, CECT 236, CIP 81.65, KCTC 3340, LMG 13354, NBRC 109769, NRRL B-14736, VTT E-97785, GIFU 9128

straininfo link

  • @ref: 83717
  • straininfo: 7696

literature

  • topic: Metabolism
  • Pubmed-ID: 24327440
  • title: 5alpha-Androst-16-en-3alpha-ol beta-D-glucuronide, precursor of 5alpha-androst-16-en-3alpha-ol in human sweat.
  • authors: Starkenmann C, Mayenzet F, Brauchli R, Troccaz M
  • journal: Chem Biodivers
  • DOI: 10.1002/cbdv.201300286
  • year: 2013
  • mesh: Androstenols/*analysis/*chemistry/metabolism, Apocrine Glands/chemistry/metabolism, Chromatography, High Pressure Liquid, Eccrine Glands/chemistry/metabolism, Female, Glucuronidase/metabolism, Glucuronides/*analysis/metabolism, Gram-Positive Bacteria/chemistry/metabolism, Humans, Male, Odorants/analysis, Spectrometry, Mass, Electrospray Ionization, Sweat/*chemistry/metabolism
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8594Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20316)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20316
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39706Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11229
44976Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 7325)https://www.ccug.se/strain?id=7325
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
83717Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID7696.1StrainInfo: A central database for resolving microbial strain identifiers
123912Curators of the CIPCollection of Institut Pasteur (CIP 81.65)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2081.65