Strain identifier

BacDive ID: 14544

Type strain: Yes

Species: Staphylococcus haemolyticus

Strain Designation: SM 131

Strain history: CIP <- 1981, J. Pillet, Inst. Pasteur, Paris, France <- DSM <- Schleifer <- Kloos: strain SM 131

NCBI tax ID(s): 1283 (species)

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General

@ref: 8672

BacDive-ID: 14544

DSM-Number: 20263

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped, human pathogen

description: Staphylococcus haemolyticus SM 131 is an aerobe, mesophilic, Gram-positive human pathogen that was isolated from human skin.

NCBI tax id

  • NCBI tax id: 1283
  • Matching level: species

strain history

@refhistory
8672<- K.H. Schleifer <- W.E. Kloos, SM 131
67770Y. Kosako <-- R. Sakazaki <-- ATCC 29970 <-- W. E. Kloos SM 131.
119771CIP <- 1981, J. Pillet, Inst. Pasteur, Paris, France <- DSM <- Schleifer <- Kloos: strain SM 131

doi: 10.13145/bacdive14544.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus haemolyticus
  • full scientific name: Staphylococcus haemolyticus Schleifer and Kloos 1975 (Approved Lists 1980)

@ref: 8672

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus haemolyticus

full scientific name: Staphylococcus haemolyticus Schleifer and Kloos 1975

strain designation: SM 131

type strain: yes

Morphology

cell morphology

  • @ref: 119771
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@reftype of hemolysishemolysis abilityincubation period
8672beta11-2 days
1197711

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8672COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8672TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
37620MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
119771CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
119771CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
8672positivegrowth37mesophilic
37620positivegrowth37mesophilic
44974positivegrowth37mesophilic
67770positivegrowth37mesophilic
119771positivegrowth22-45
119771nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
44974aerobe
119771facultative anaerobe

murein

  • @ref: 8672
  • murein short key: A11.03
  • type: A3alpha L-Lys-Gly2-4-L-Ser1-2-Gly

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
119771hippurate+hydrolysis606565
119771nitrate+reduction17632
119771nitrite-reduction16301
68375urea-hydrolysis16199
68375ornithine-degradation18257
68375esculin-hydrolysis4853
68375D-glucose+fermentation17634
68375D-fructose-fermentation15824
68375D-mannose-fermentation16024
68375maltose+fermentation17306
68375trehalose+fermentation27082
68375D-mannitol-fermentation16899
68375raffinose-fermentation16634
68375cellobiose-fermentation17057
68375nitrate+reduction17632
68375sucrose+fermentation17992

antibiotic resistance

  • @ref: 68375
  • ChEBI: 28368
  • metabolite: novobiocin
  • is antibiotic: yes
  • is sensitive: yes
  • sensitivity conc.: 1.8 µg

metabolite production

@refChebi-IDmetaboliteproduction
6837515688acetoinyes
11977135581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
6837515688acetoin+
11977115688acetoin+
11977117234glucose+

enzymes

@refvalueactivityec
8672catalase+1.11.1.6
8672cytochrome-c oxidase-1.9.3.1
68375beta-glucuronidase+3.2.1.31
68375L-arginine arylamidase-
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375urease-3.5.1.5
119771oxidase-
119771beta-galactosidase-3.2.1.23
119771alcohol dehydrogenase+1.1.1.1
119771gelatinase-
119771amylase-
119771DNase-
119771caseinase-3.4.21.50
119771catalase+1.11.1.6
119771coagulase-
119771tween esterase-
119771lecithinase-
119771lipase-
119771lysine decarboxylase-4.1.1.18
119771ornithine decarboxylase-4.1.1.17
119771phenylalanine ammonia-lyase-4.3.1.24
119771protease-
119771urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119771--++------+---+-----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119771----+/-----++----------+-----++-++-+/------+/----------

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
8672-+--+--+-+----++--+/-+-++/---+
8672-+--+--+-+----++---+-++--+
8672-+--+--+-+----++---+-+++++
8672-+--+--++/-+----++---+-+++-+
8672-+--+--+++--+/--++---+-+++-+
8672----+--+-+----++---+/--++--+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119771+--+---+-++--------+-----------+---+--+--------------------++--------------+----------+---+---+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
8672human skin
44974Human skinN.C.,Raleigh or USA,N.J.,BrunswickUSAUSANorth America
67770Human skin
119771Human, Skin

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Organ#Skin, Nail, Hair

taxonmaps

  • @ref: 69479
  • File name: preview.99_178.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3;97_3;98_3;99_178&stattab=map
  • Last taxonomy: Staphylococcus
  • 16S sequence: LC383923
  • Sequence Identity:
  • Total samples: 261
  • soil counts: 23
  • aquatic counts: 6
  • animal counts: 232

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
8672yesyes2Risk group (German classification)
1197711Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus haemolyticus strain DSM 20263 16S ribosomal RNA gene, partial sequenceAY688060464ena1283
20218Staphylococcus haemolyticus 16S ribosomal RNA (16S rRNA) geneL376001468ena1283
20218S.haemolyticus gene for 16S ribosomal RNAZ26896771ena1283
8672S.haemolyticus gene for 16S ribosomal RNAX661001544ena1283
8672Staphylococcus haemolyticus gene for 16S rRNA, partial sequence, strain: ATCC 29970 (= MAFF 911476)D833671475ena1283
67770Staphylococcus haemolyticus JCM 2416 gene for 16S ribosomal RNA, partial sequenceLC3839231473ena1283

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Staphylococcus haemolyticus ATCC 29970GCA_006094395completencbi1283
66792Staphylococcus haemolyticus NBRC 109768GCA_007992795contigncbi1283
66792Staphylococcus haemolyticus strain ATCC 299701283.682completepatric1283
66792Staphylococcus haemolyticus strain NBRC 1097681283.729wgspatric1283
66792Staphylococcus haemolyticus strain NCTC 110421283.594wgspatric1283
66792Staphylococcus haemolyticus strain NCTC110421283.646wgspatric1283
67770Staphylococcus haemolyticus NCTC 11042GCA_002901805scaffoldncbi1283
67770Staphylococcus haemolyticus NCTC11042GCA_900458595contigncbi1283

GC content

@refGC-contentmethod
867236.4
6777036.4thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno88.842no
flagellatedno92.969no
gram-positiveyes91.323no
anaerobicno99.199no
aerobicyes90.85yes
halophileyes95.662no
spore-formingno90.651no
thermophileno99.725yes
glucose-utilyes88.867no
glucose-fermentyes82.355yes

External links

@ref: 8672

culture collection no.: DSM 20263, ATCC 29970, CCM 2737, CCUG 7323, NCTC 11042, JCM 2416, BCRC 12923, CECT 4900, CIP 81.56, KCTC 3341, LMG 13349, NBRC 109768, NCIMB 14489, NRRL B-14755, VTT E-97770, GIFU 9124

straininfo link

  • @ref: 83675
  • straininfo: 7697

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism243274405alpha-Androst-16-en-3alpha-ol beta-D-glucuronide, precursor of 5alpha-androst-16-en-3alpha-ol in human sweat.Starkenmann C, Mayenzet F, Brauchli R, Troccaz MChem Biodivers10.1002/cbdv.2013002862013Androstenols/*analysis/*chemistry/metabolism, Apocrine Glands/chemistry/metabolism, Chromatography, High Pressure Liquid, Eccrine Glands/chemistry/metabolism, Female, Glucuronidase/metabolism, Glucuronides/*analysis/metabolism, Gram-Positive Bacteria/chemistry/metabolism, Humans, Male, Odorants/analysis, Spectrometry, Mass, Electrospray Ionization, Sweat/*chemistry/metabolismPathogenicity
Pathogenicity28962025Characterization of two novel variants of staphylococcal cassette chromosome mec elements in oxacillin-resistant Staphylococcus lugdunensis.Chang SC, Lee MH, Yeh CF, Liu TP, Lin JF, Ho CM, Lu JJJ Antimicrob Chemother10.1093/jac/dkx2912017Anti-Bacterial Agents/*pharmacology, *Chromosomes, Bacterial, Evolution, Molecular, *Gene Order, Genes, Bacterial, Humans, Oxacillin/*pharmacology, Recombination, Genetic, Staphylococcal Infections/microbiology, Staphylococcus lugdunensis/*drug effects/*genetics/isolation & purification, Whole Genome Sequencing, *beta-Lactam ResistanceEnzymology
Pathogenicity35513687Application of pulse-modulated radio-frequency atmospheric pressure glow discharge for degradation of doxycycline from a flowing liquid solution.Dzimitrowicz A, Caban M, Terefinko D, Pohl P, Jamroz P, Babinska W, Cyganowski P, Stepnowski P, Lojkowska E, Sledz W, Motyka-Pomagruk ASci Rep10.1038/s41598-022-11088-w2022Anti-Bacterial Agents/pharmacology, Atmospheric Pressure, *Body Fluids, *Doxycycline/pharmacology, Escherichia coli, Humans, NitrogenMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8672Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20263)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20263
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37620Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/11219
44974Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 7323)https://www.ccug.se/strain?id=7323
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
83675Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID7697.1StrainInfo: A central database for resolving microbial strain identifiers
119771Curators of the CIPCollection of Institut Pasteur (CIP 81.56)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2081.56