Strain identifier

BacDive ID: 1452

Type strain: Yes

Species: Geobacillus jurassicus

Strain Designation: DS1, R-35651

Strain history: <- T. N. Nazina; DS1 <- D. Sh. Sokolova

NCBI tax ID(s): 1223502 (strain), 235932 (species)

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General

@ref: 6084

BacDive-ID: 1452

DSM-Number: 15726

keywords: 16S sequence, Bacteria, aerobe, chemoorganotroph, spore-forming, thermophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Geobacillus jurassicus DS1 is an aerobe, chemoorganotroph, spore-forming bacterium that forms circular colonies and was isolated from formation water of the Dayang oil field .

NCBI tax id

NCBI tax idMatching level
1223502strain
235932species

strain history

  • @ref: 6084
  • history: <- T. N. Nazina; DS1 <- D. Sh. Sokolova

doi: 10.13145/bacdive1452.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Geobacillus
  • species: Geobacillus jurassicus
  • full scientific name: Geobacillus jurassicus Nazina et al. 2005

@ref: 6084

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Geobacillus

species: Geobacillus jurassicus

full scientific name: Geobacillus jurassicus Nazina et al. 2005 emend. Coorevits et al. 2012

strain designation: DS1, R-35651

type strain: yes

Morphology

cell morphology

  • @ref: 23042
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: peritrichous

colony morphology

  • @ref: 23042
  • colony size: 2.0 mm
  • colony color: colourless
  • colony shape: circular
  • incubation period: 1-2 days
  • medium used: nutrient agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6084PLATE COUNT AGAR (DSMZ Medium 464)yeshttps://mediadive.dsmz.de/medium/464Name: PLATE COUNT AGAR (DSMZ Medium 464) Composition: Agar 15.0 g/l Tryptone 5.0 g/l Yeast extract 2.5 g/l Dextrose 1.0 g/l Distilled water
23042Nutrient agar (NA)yes
23042trypticase soy agaryes

culture temp

@refgrowthtypetemperaturerange
6084positivegrowth55thermophilic
23042positivegrowth45.0-65.0thermophilic
23042positiveoptimum58.0-60.0thermophilic

culture pH

@refabilitytypepHPH range
23042positivegrowth5.0-9.0alkaliphile
23042positiveoptimum7.0-7.2

Physiology and metabolism

oxygen tolerance

  • @ref: 23042
  • oxygen tolerance: aerobe

nutrition type

  • @ref: 23042
  • type: chemoorganotroph

spore formation

  • @ref: 23042
  • spore description: ellipsoidal,subterminal,in slightly swollen sporangia
  • type of spore: spore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
23042NaClpositivegrowth5.0-5.5 %(w/v)
23042NaClpositivegrowth1.0 %(w/v)
23042NaClpositivegrowth5.0 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
23042168082-dehydro-D-gluconate-builds acid from
23042174265-dehydro-D-gluconate-builds acid from
2304215963ribitol-builds acid from
2304227613amygdalin-builds acid from
2304218305arbutin-builds acid from
2304217108D-arabinose-builds acid from
2304218333D-arabitol-builds acid from
2304228847D-fucose-builds acid from
2304262318D-lyxose-builds acid from
2304216443D-tagatose-builds acid from
2304216813galactitol-builds acid from
2304217113erythritol-builds acid from
2304228066gentiobiose-builds acid from
2304224265gluconate-builds acid from
2304228087glycogen-builds acid from
2304215443inulin-builds acid from
2304218403L-arabitol-builds acid from
2304218287L-fucose-builds acid from
2304217266L-sorbose-builds acid from
2304265328L-xylose-builds acid from
2304217716lactose-builds acid from
2304228053melibiose-builds acid from
2304217268myo-inositol-builds acid from
23042506227N-acetylglucosamine-builds acid from
2304216634raffinose-builds acid from
2304226546rhamnose-builds acid from
2304217814salicin-builds acid from
2304230911sorbitol-builds acid from
2304217151xylitol-builds acid from
2304217057cellobiose-builds gas from
2304215824D-fructose-builds gas from
2304217634D-glucose-builds gas from
2304216024D-mannose-builds gas from
2304265327D-xylose-builds gas from
2304228260galactose-builds gas from
2304217754glycerol-builds gas from
2304230849L-arabinose-builds gas from
2304217306maltose-builds gas from
2304229864mannitol-builds gas from
230426731melezitose-builds gas from
2304237657methyl D-glucoside-builds gas from
2304233942ribose-builds gas from
2304228017starch-builds gas from
2304217992sucrose-builds gas from
2304227082trehalose-builds gas from
2304232528turanose-builds gas from
2304216947citrate-carbon source
2304218186tyrosine-degradation
2304229033ferrous ion-electron acceptor
2304227897tryptophan-energy source
2304217234glucose-fermentation
2304229985L-glutamate-growth
2304216449alanine-growth
2304228885butanol-growth
2304215740formate-growth
2304246645isobutanol-growth
2304217790methanol-growth
2304215882phenol-growth
2304218401phenylacetate-growth
2304228831propanol-growth
2304217272propionate-growth
2304217822serine-growth
23042O-nitrophenyl-beta-D-galactopyranosid-hydrolysis
2304217057cellobiose+builds acid from
2304215824D-fructose+builds acid from
2304217634D-glucose+builds acid from
2304216024D-mannose+builds acid from
2304265327D-xylose+builds acid from
2304228260galactose+builds acid from
2304217754glycerol+builds acid from
2304230849L-arabinose+builds acid from
2304217306maltose+builds acid from
2304229864mannitol+builds acid from
230426731melezitose+builds acid from
2304237657methyl D-glucoside+builds acid from
2304233942ribose+builds acid from
2304228017starch+builds acid from
2304217992sucrose+builds acid from
2304227082trehalose+builds acid from
2304232528turanose+builds acid from
23042nutrient broth+growth
23042potato agar+growth
23042casein+hydrolysis
230424853esculin+hydrolysis
230425291gelatin+hydrolysis
2304228017starch+hydrolysis
2304217632nitrate+reduction

metabolite production

@refChebi-IDmetaboliteproduction
2304235581indoleno
2304216136hydrogen sulfideno
2304215688acetoinno
2304216016dihydroxyacetoneyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-testindole testcitrate test
2304215688acetoin-
2304217234glucose-
2304235581indole-
2304216947citrate-

enzymes

@refvalueactivityec
23042arginine dihydrolase-3.5.3.6
23042beta-galactosidase-3.2.1.23
23042catalase+1.11.1.6
23042cytochrome oxidase+1.9.3.1
23042lecithinase-
23042lysine decarboxylase-4.1.1.18
23042ornithine decarboxylase-4.1.1.17
23042tryptophan deaminase-4.1.99.1
23042urease-3.5.1.5

Isolation, sampling and environmental information

isolation

  • @ref: 6084
  • sample type: formation water of the Dayang oil field (Kongdian area)
  • geographic location: Hebei Province
  • country: China
  • origin.country: CHN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Oil reservoir
#Environmental#Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_4517.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_79;96_191;97_207;98_231;99_4517&stattab=map
  • Last taxonomy: Geobacillus
  • 16S sequence: FN428697
  • Sequence Identity:
  • Total samples: 426
  • soil counts: 44
  • aquatic counts: 94
  • animal counts: 263
  • plant counts: 25

Safety information

risk assessment

  • @ref: 6084
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Geobacillus jurassicus strain DSM 15726 16S-23S ribosomal RNA intergenic spacer and tRNA-Ile gene, partial sequence; and tRNA-Ala gene, complete sequenceEU157943349ena1223502
6084Geobacillus jurassicus strain DS1 16S ribosomal RNA gene, partial sequenceAY3124041520ena235932
6084Geobacillus jurassicus partial 16S rRNA gene, strain R-35651FN4286971510ena1223502

GC content

  • @ref: 6084
  • GC-content: 54.5
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 6084

culture collection no.: DSM 15726, LMG 23069, VKM B-2301

straininfo link

  • @ref: 71101
  • straininfo: 138983

literature

  • topic: Phylogeny
  • Pubmed-ID: 15709364
  • title: Geobacillus jurassicus sp. nov., a new thermophilic bacterium isolated from a high-temperature petroleum reservoir, and the validation of the Geobacillus species.
  • authors: Nazina TN, Sokolova DSh, Grigoryan AA, Shestakova NM, Mikhailova EM, Poltaraus AB, Tourova TP, Lysenko AM, Osipov GA, Belyaev SS
  • journal: Syst Appl Microbiol
  • DOI: 10.1016/j.syapm.2004.09.001
  • year: 2005
  • mesh: Aerobiosis, Bacillaceae/*classification/cytology/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Carbohydrate Metabolism, Carboxylic Acids/metabolism, China, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Environmental Microbiology, Fatty Acids/analysis/isolation & purification, Genes, rRNA, Gentian Violet, Hydrocarbons/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Petroleum/metabolism/*microbiology, Phenazines, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spores, Bacterial/cytology, Temperature
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6084Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15726)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15726
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23042An Coorevits,Anna E. Dinsdale,Gillian Halket,Liesbeth Lebbe,Paul De Vos,Anita Van Landschoot,Niall A. Logan10.1099/ijs.0.030346-0Taxonomic revision of the genus Geobacillus : emendation of Geobacillus , G. stearothermophilus , G. jurassicus , G. toebii , G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly 'thermoglucosidasius'); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus comb. nov.; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans comb. nov.; and proposal of Anoxybacillus caldiproteolyticus sp. nov.IJSEM 62: 1470-1485 201221856988
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71101Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID138983.1StrainInfo: A central database for resolving microbial strain identifiers