Geobacillus jurassicus (DSM 15726, LMG 23069, VKM B-2301)

Name: Geobacillus jurassicus (DSM 15726, LMG 23069, VKM B-2301)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1452

Type strain

Strain Designation DS1, R-35651

Culture col. no. DSM 15726 LMG 23069 VKM B-2301 DS1 R-35651

NCBI tax ID(s) 1223502 235932

Special Collections DSMZ Literature strains

History <- T. N. Nazina; DS1 <- D. Sh. Sokolova

Links

Geobacillus jurassicus DS1 is an aerobe, chemoorganotroph, spore-forming prokaryote that forms circular colonies and was isolated from formation water of the Dayang oil field .

spore-forming Gram-positive motile rod-shaped colony-forming aerobe chemoorganotroph thermophilic 16S sequence

Name and taxonomic classification

SI-ID 138983

NBRC 107829,CCM 7224,DSM 15726,VKM B-2301,LMG 23069,BCRC 17566,CCRC 17566

@ref 20215

Last LPSN update 2026-02-09 11:18:06

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Geobacillus

Species Geobacillus jurassicus

Full scientific name Geobacillus jurassicus Nazina et al. 2005

BacDive ID	Other strains from **Geobacillus jurassicus** (1)	Type strain
1453	G. jurassicus DS2, R-32597, DSM 15727

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Flagellum arrangement
23042	positive	rod-shaped		peritrichous

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
23042	2.0 mm	colourless	circular	1-2 days	nutrient agar

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6084	PLATE COUNT AGAR (DSMZ Medium 464)	Medium recipe at MediaDive	Name: PLATE COUNT AGAR (DSMZ Medium 464) Composition: Agar 15.0 g/l Tryptone 5.0 g/l Yeast extract 2.5 g/l Dextrose 1.0 g/l Distilled water
23042	Nutrient agar (NA)
23042	trypticase soy agar

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
6084	positive	growth	55	thermophilic
23042	positive	growth	45.0-65.0	thermophilic
23042	positive	optimum	58.0-60.0	thermophilic

Culture pH

@ref	Ability	Type	PH	PH range
23042	positive	optimum	7.0-7.2
23042	positive	growth	5.0-9.0	alkaliphile

Physiology and metabolism

Oxygen tolerance

23042

Oxygen toleranceaerobe

Nutrition type

23042

Typechemoorganotroph

Spore formation

@ref	Spore description	Type of spore	Spore formation
23042	ellipsoidal,subterminal,in slightly swollen sporangia	spore

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23042	NaCl	positive	growth	5.0-5.5 %(w/v)
23042	NaCl	positive	growth	1.0 %(w/v)
23042	NaCl	positive	growth	5.0 %(w/v)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23042	16808 ChEBI	2-dehydro-D-gluconate	-	builds acid from
23042	17426 ChEBI	5-dehydro-D-gluconate	-	builds acid from
23042	16449 ChEBI	alanine	-	growth
23042	27613 ChEBI	amygdalin	-	builds acid from
23042	18305 ChEBI	arbutin	-	builds acid from
23042	28885 ChEBI	butanol	-	growth
23042		casein	+	hydrolysis
23042	17057 ChEBI	cellobiose	-	builds gas from
23042	17057 ChEBI	cellobiose	+	builds acid from
23042	16947 ChEBI	citrate	-	carbon source
23042	17108 ChEBI	D-arabinose	-	builds acid from
23042	18333 ChEBI	D-arabitol	-	builds acid from
23042	15824 ChEBI	D-fructose	-	builds gas from
23042	15824 ChEBI	D-fructose	+	builds acid from
23042	28847 ChEBI	D-fucose	-	builds acid from
23042	17634 ChEBI	D-glucose	-	builds gas from
23042	17634 ChEBI	D-glucose	+	builds acid from
23042	62318 ChEBI	D-lyxose	-	builds acid from
23042	16024 ChEBI	D-mannose	-	builds gas from
23042	16024 ChEBI	D-mannose	+	builds acid from
23042	16443 ChEBI	D-tagatose	-	builds acid from
23042	65327 ChEBI	D-xylose	-	builds gas from
23042	65327 ChEBI	D-xylose	+	builds acid from
23042	17113 ChEBI	erythritol	-	builds acid from
23042	4853 ChEBI	esculin	+	hydrolysis
23042	29033 ChEBI	ferrous ion	-	electron acceptor
23042	15740 ChEBI	formate	-	growth
23042	16813 ChEBI	galactitol	-	builds acid from
23042	28260 ChEBI	galactose	-	builds gas from
23042	28260 ChEBI	galactose	+	builds acid from
23042	5291 ChEBI	gelatin	+	hydrolysis
23042	28066 ChEBI	gentiobiose	-	builds acid from
23042	24265 ChEBI	gluconate	-	builds acid from
23042	17234 ChEBI	glucose	-	fermentation
23042	17754 ChEBI	glycerol	-	builds gas from
23042	17754 ChEBI	glycerol	+	builds acid from
23042	28087 ChEBI	glycogen	-	builds acid from
23042	15443 ChEBI	inulin	-	builds acid from
23042	46645 ChEBI	isobutanol	-	growth
23042	30849 ChEBI	L-arabinose	-	builds gas from
23042	30849 ChEBI	L-arabinose	+	builds acid from
23042	18403 ChEBI	L-arabitol	-	builds acid from
23042	18287 ChEBI	L-fucose	-	builds acid from
23042	29985 ChEBI	L-glutamate	-	growth
23042	17266 ChEBI	L-sorbose	-	builds acid from
23042	65328 ChEBI	L-xylose	-	builds acid from
23042	17716 ChEBI	lactose	-	builds acid from
23042	17306 ChEBI	maltose	-	builds gas from
23042	17306 ChEBI	maltose	+	builds acid from
23042	29864 ChEBI	mannitol	-	builds gas from
23042	29864 ChEBI	mannitol	+	builds acid from
23042	6731 ChEBI	melezitose	-	builds gas from
23042	6731 ChEBI	melezitose	+	builds acid from
23042	28053 ChEBI	melibiose	-	builds acid from
23042	17790 ChEBI	methanol	-	growth
23042	37657 ChEBI	methyl D-glucoside	-	builds gas from
23042	37657 ChEBI	methyl D-glucoside	+	builds acid from
23042	17268 ChEBI	myo-inositol	-	builds acid from
23042	506227 ChEBI	N-acetylglucosamine	-	builds acid from
23042	17632 ChEBI	nitrate	+	reduction
23042		nutrient broth	+	growth
23042		O-nitrophenyl-beta-D-galactopyranosid	-	hydrolysis
23042	15882 ChEBI	phenol	-	growth
23042	18401 ChEBI	phenylacetate	-	growth
23042		potato agar	+	growth
23042	28831 ChEBI	propanol	-	growth
23042	17272 ChEBI	propionate	-	growth
23042	16634 ChEBI	raffinose	-	builds acid from
23042	26546 ChEBI	rhamnose	-	builds acid from
23042	15963 ChEBI	ribitol	-	builds acid from
23042	33942 ChEBI	ribose	-	builds gas from
23042	33942 ChEBI	ribose	+	builds acid from
23042	17814 ChEBI	salicin	-	builds acid from
23042	17822 ChEBI	serine	-	growth
23042	30911 ChEBI	sorbitol	-	builds acid from
23042	28017 ChEBI	starch	-	builds gas from
23042	28017 ChEBI	starch	+	builds acid from
23042	28017 ChEBI	starch	+	hydrolysis
23042	17992 ChEBI	sucrose	-	builds gas from
23042	17992 ChEBI	sucrose	+	builds acid from
23042	27082 ChEBI	trehalose	-	builds gas from
23042	27082 ChEBI	trehalose	+	builds acid from
23042	27897 ChEBI	tryptophan	-	energy source
23042	32528 ChEBI	turanose	-	builds gas from
23042	32528 ChEBI	turanose	+	builds acid from
23042	18186 ChEBI	tyrosine	-	degradation
23042	17151 ChEBI	xylitol	-	builds acid from

Metabolite production

@ref	Chebi-ID	Metabolite
23042	15688 ChEBI	acetoin
23042	16016 ChEBI	dihydroxyacetone
23042	16136 ChEBI	hydrogen sulfide
23042	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Citrate test	Methylred-test	Indole test
23042	15688 ChEBI	acetoin	-
23042	16947 ChEBI	citrate		-
23042	17234 ChEBI	glucose			-
23042	35581 ChEBI	indole				-

Enzymes

@ref	Value	Activity	Ec
23042	arginine dihydrolase	-	3.5.3.6
23042	beta-galactosidase	-	3.2.1.23
23042	catalase	+	1.11.1.6
23042	cytochrome oxidase	+	1.9.3.1
23042	lecithinase	-
23042	lysine decarboxylase	-	4.1.1.18
23042	ornithine decarboxylase	-	4.1.1.17
23042	tryptophan deaminase	-	4.1.99.1
23042	urease	-	3.5.1.5

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Oil reservoir
#Environmental	#Aquatic	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6084	formation water of the Dayang oil field (Kongdian area)	Hebei Province	China	CHN	Asia

Taxonmaps

69479

Global distribution of 16S sequence FN428697 (>99% sequence identity) for Geobacillus from Microbeatlas

Aquatic Samples	94
Soil Samples	44
Animal Samples	263
Plant Samples	25
Total Samples	426

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6084	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Geobacillus jurassicus strain DSM 15726 16S-23S ribosomal RNA intergenic spacer and tRNA-Ile gene, partial sequence; and tRNA-Ala gene, complete sequence	EU157943	349	1223502
6084	Geobacillus jurassicus strain DS1 16S ribosomal RNA gene, partial sequence	AY312404	1520	235932
6084	Geobacillus jurassicus partial 16S rRNA gene, strain R-35651	FN428697	1510	1223502

GC content

@ref	GC-content (mol%)	Method
6084	54.5	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Computer-aided rational design strategy based on protein surface charge to improve the thermal stability of a novel esterase from Geobacillus jurassicus	Song R, Zhang J, Zhu M, Lin L, Wei W, Wei D.	Biotechnol Lett.		2024
Enzymology	Computer-aided rational design strategy based on protein surface charge to improve the thermal stability of a novel esterase from Geobacillus jurassicus.	Song R, Zhang J, Zhu M, Lin L, Wei W, Wei D.	Biotechnol Lett	10.1007/s10529-024-03473-4	2024
Phylogeny	Integrated Proteomic and Molecular Identification of Thermophilic Geobacillus Strains from Algerian Desert Sands and Their Enzymatic Potential	Hammadi A, Merzoug M, Aireche M, Zater Z, Bendida K, Brakna C, Choubane S, Todorov S, Saidi D.	Life (Basel)		2025
Phylogeny	Taxonomic Diversity and Antimicrobial Potential of Thermophilic Bacteria from Two Extreme Algerian Hot Springs.	Aireche M, Merzoug M, Hammadi AI, Zater ZY, Bendida K, Brakna CN, Berrazeg M, Aireche AY, Saidi Y, Todorov SD, Arabet D, Saidi D.	Microorganisms	10.3390/microorganisms13061425	2025
Enzymology	Functional Characterization, Genome Assembly, and Annotation of Geobacillus sp. G4 Isolated from a Geothermal Field in Tacna, Peru.	Ticona ARP, Santos KCR, Ramirez-Arua HE, Castellanos R, Silva JP, Hamann PRV, Noronha EF, Lopes FAC.	Microorganisms	10.3390/microorganisms13061374	2025
Enzymology	Microbial Biodegradation of Paraffin Wax in Malaysian Crude Oil Mediated by Degradative Enzymes.	Adlan NA, Sabri S, Masomian M, Ali MSM, Rahman RNZRA.	Front Microbiol	10.3389/fmicb.2020.565608	2020
Phylogeny	Bioremediation of Petroleum Hydrocarbons Using Acinetobacter sp. SCYY-5 Isolated from Contaminated Oil Sludge: Strategy and Effectiveness Study.	Cai Y, Wang R, Rao P, Wu B, Yan L, Hu L, Park S, Ryu M, Zhou X.	Int J Environ Res Public Health	10.3390/ijerph18020819	2021
Phylogeny	[The phylogenetic diversity of aerobic organotrophic bacteria from the Dagan high-temperature oil field].	Nazina TN, Sokolova DSh, Shestakova NM, Grigor'ian AA, Mikhailova EM, Babich TL, Lysenko AM, Turova TP, Poltaraus AB, Feng T, Ni F, Beliaev SS.	Mikrobiologiia	10.1007/s11021-005-0073-0	2005
	Creation of an Online Platform for Identification of Microorganisms: Peak Picking or Full-Spectrum Analysis.	Starostin KV, Demidov EA, Ershov NI, Bryanskaya AV, Efimov VM, Shlyakhtun VN, Peltek SE.	Front Microbiol	10.3389/fmicb.2020.609033	2020
	Population expansions shared among coexisting bacterial lineages are revealed by genetic evidence.	Avitia M, Escalante AE, Rebollar EA, Moreno-Letelier A, Eguiarte LE, Souza V.	PeerJ	10.7717/peerj.696	2014
Enzymology	Primers for amplification of nitrous oxide reductase genes associated with Firmicutes and Bacteroidetes in organic-compound-rich soils.	Jung J, Choi S, Jung H, Scow KM, Park W.	Microbiology (Reading)	10.1099/mic.0.060194-0	2013
Phylogeny	Diversity of Metabolically Active Bacteria in Water-Flooded High-Temperature Heavy Oil Reservoir.	Nazina TN, Shestakova NM, Semenova EM, Korshunova AV, Kostrukova NK, Tourova TP, Min L, Feng Q, Poltaraus AB.	Front Microbiol	10.3389/fmicb.2017.00707	2017
Phylogeny	Taxonomic revision of the genus Geobacillus: emendation of Geobacillus, G. stearothermophilus, G. jurassicus, G. toebii, G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly 'thermoglucosidasius'); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus comb. nov.; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans comb. nov.; and proposal of Anoxybacillus caldiproteolyticus sp. nov.	Coorevits A, Dinsdale AE, Halket G, Lebbe L, De Vos P, Van Landschoot A, Logan NA.	Int J Syst Evol Microbiol	10.1099/ijs.0.030346-0	2012
Enzymology	Geobacillus zalihae sp. nov., a thermophilic lipolytic bacterium isolated from palm oil mill effluent in Malaysia.	Abd Rahman RN, Leow TC, Salleh AB, Basri M.	BMC Microbiol	10.1186/1471-2180-7-77	2007
Phylogeny	Geobacillus jurassicus sp. nov., a new thermophilic bacterium isolated from a high-temperature petroleum reservoir, and the validation of the Geobacillus species.	Nazina TN, Sokolova DSh, Grigoryan AA, Shestakova NM, Mikhailova EM, Poltaraus AB, Tourova TP, Lysenko AM, Osipov GA, Belyaev SS	Syst Appl Microbiol	10.1016/j.syapm.2004.09.001	2005

References

#6084	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15726
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#23042	An Coorevits,Anna E. Dinsdale,Gillian Halket,Liesbeth Lebbe,Paul De Vos,Anita Van Landschoot,Niall A. Logan: Taxonomic revision of the genus Geobacillus : emendation of Geobacillus , G. stearothermophilus , G. jurassicus , G. toebii , G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly 'thermoglucosidasius'); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus comb. nov.; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans comb. nov.; and proposal of Anoxybacillus caldiproteolyticus sp. nov.. IJSEM 62: 1470 - 1485 2012 ( DOI 10.1099/ijs.0.030346-0 , PubMed 21856988 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Geobacillus jurassicus (DSM 15726, LMG 23069, VKM B-2301)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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