Strain identifier

BacDive ID: 14503

Type strain: Yes

Species: Staphylococcus auricularis

Strain Designation: WK 811M, WK811M, LRA 013 01 83

Strain history: CIP <- 1992, R. Mercier, bioMérieux, La Balme-les-Grottes, France: strain LRA 013 01 83 <- North Carolina State Inst. <- W.E. Kloos: strain WK811M

NCBI tax ID(s): 29379 (species)

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General

@ref: 8957

BacDive-ID: 14503

DSM-Number: 20609

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, Gram-positive, coccus-shaped

description: Staphylococcus auricularis WK 811M is an obligate aerobe, Gram-positive, coccus-shaped bacterium that was isolated from human external ear.

NCBI tax id

  • NCBI tax id: 29379
  • Matching level: species

strain history

@refhistory
8957<- K.H. Schleifer <- W.E. Kloos, WK 811M
67770ATCC 33753 <-- W. E. Kloos WK811M.
116634CIP <- 1992, R. Mercier, bioMérieux, La Balme-les-Grottes, France: strain LRA 013 01 83 <- North Carolina State Inst. <- W.E. Kloos: strain WK811M

doi: 10.13145/bacdive14503.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus auricularis
  • full scientific name: Staphylococcus auricularis Kloos and Schleifer 1983

@ref: 8957

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus auricularis

full scientific name: Staphylococcus auricularis Kloos and Schleifer 1983

strain designation: WK 811M, WK811M, LRA 013 01 83

type strain: yes

Morphology

cell morphology

  • @ref: 116634
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@reftype of hemolysisincubation period
8957gamma1-2 days
116634

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8957COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8957TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
34366MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
116634CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
116634CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
8957positivegrowth37
34366positivegrowth37
67770positivegrowth37
116634positivegrowth30-41
116634nogrowth10
116634nogrowth22

Physiology and metabolism

oxygen tolerance

  • @ref: 116634
  • oxygen tolerance: obligate aerobe

murein

  • @ref: 8957
  • murein short key: A11.03
  • type: A3alpha L-Lys-Gly2-4-L-Ser1-2-Gly

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
116634hippurate-hydrolysis606565
116634nitrate-reduction17632
116634nitrite-reduction16301
68375urea-hydrolysis16199
68375arginine-hydrolysis29016
68375ornithine-degradation18257
68375esculin-hydrolysis4853
68375D-glucose+fermentation17634
68375D-fructose+fermentation15824
68375D-mannose-fermentation16024
68375maltose+fermentation17306
68375lactose-fermentation17716
68375trehalose+fermentation27082
68375raffinose-fermentation16634
68375nitrate-reduction17632

antibiotic resistance

  • @ref: 68375
  • ChEBI: 28368
  • metabolite: novobiocin
  • is antibiotic: yes
  • is sensitive: yes
  • sensitivity conc.: 1.8 µg

metabolite production

  • @ref: 116634
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11663415688acetoin-
11663417234glucose-

enzymes

@refvalueactivityec
8957catalase+1.11.1.6
8957cytochrome-c oxidase-1.9.3.1
68375pyrrolidonyl arylamidase-3.4.19.3
68375alkaline phosphatase-3.1.3.1
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase-3.5.3.6
68375urease-3.5.1.5
116634oxidase-
116634alcohol dehydrogenase-1.1.1.1
116634gelatinase-
116634amylase-
116634DNase-
116634caseinase-3.4.21.50
116634catalase+1.11.1.6
116634coagulase-
116634tween esterase-
116634gamma-glutamyltransferase-2.3.2.2
116634lecithinase-
116634lipase+
116634lysine decarboxylase-4.1.1.18
116634ornithine decarboxylase-4.1.1.17
116634phenylalanine ammonia-lyase-4.3.1.24
116634protease-
116634urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116634-+++-+--+-++-+-+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116634+/----------+/-+/----------------+/----+/------------------

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
8957----++-+-+-------+---+/-----
8957----++-+-+-------+---+++/---
8957----++-+-+-------+---+++/-+-
8957----++-+-++-++-+++---+++++
8957----++-+-+-------+/---------

Isolation, sampling and environmental information

isolation

@refsample type
8957human external ear
67770External auditory meatus
116634Human, External auditory meatus

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Organ#Ear

taxonmaps

  • @ref: 69479
  • File name: preview.99_8858.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3;97_3;98_6476;99_8858&stattab=map
  • Last taxonomy: Staphylococcus
  • 16S sequence: LC383924
  • Sequence Identity:
  • Total samples: 46266
  • soil counts: 1633
  • aquatic counts: 2688
  • animal counts: 40900
  • plant counts: 1045

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
89571Risk group (German classification)
1166341Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Staphylococcus auricularis strain DSM 20609 16S ribosomal RNA gene, partial sequenceAY688030464nuccore29379
20218Staphylococcus auricularis 16S ribosomal RNA gene, partial sequenceL375981470nuccore29379
20218Staphylococcus auricularis ATCC 33753 16S rRNA gene, partial sequenceU02911391nuccore29379
8957Staphylococcus auricularis gene for 16S rRNA, partial sequence, strain: ATCC 33753 (= MAFF 911484)D833581475nuccore29379
67770Staphylococcus auricularis JCM 2421 gene for 16S ribosomal RNA, partial sequenceLC3839241473nuccore29379
124043S.auricularis gene for 16S ribosomal RNAZ26889771nuccore29379

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Staphylococcus auricularis NCTC12101GCA_900478415completencbi29379
66792Staphylococcus auricularis JCM 2421GCA_018323945completencbi29379
66792Staphylococcus auricularis strain DSM 2060929379.8wgspatric29379
66792Staphylococcus auricularis strain FDAARGOS_88229379.37completepatric29379
66792Staphylococcus auricularis strain JCM 242129379.39completepatric29379
66792Staphylococcus auricularis strain NCTC 1210129379.16wgspatric29379
66792Staphylococcus auricularis strain NCTC1210129379.19completepatric29379
66792Staphylococcus auricularis DSM 206092724678964draftimg29379
67770Staphylococcus auricularis DSM 20609GCA_001500315contigncbi29379
67770Staphylococcus auricularis NCTC 12101GCA_002902455scaffoldncbi29379

GC content

  • @ref: 8957
  • GC-content: 38.8

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingyes89.189no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no97.267yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes67.985yes
69480spore-formingspore-formingAbility to form endo- or exosporesno74.027no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno95.977yes
69480flagellatedmotile2+Ability to perform flagellated movementno81.121no

External links

@ref: 8957

culture collection no.: DSM 20609, ATCC 33753, JCM 2421, BCRC 13912, CCM 3991, CCUG 15601, CCUG 36974, CECT 4052, CIP 103587, KCTC 3584, NCTC 12101, NRRL B-14762

straininfo link

  • @ref: 83640
  • straininfo: 40671

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8957Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20609)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20609
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34366Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15401
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
83640Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40671.1StrainInfo: A central database for resolving microbial strain identifiers
116634Curators of the CIPCollection of Institut Pasteur (CIP 103587)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103587
124043Dr. Isabel Schober, Dr. Julia KoblitzData extracted from sequence databases, automatically matched based on designation and taxonomy