Strain identifier
BacDive ID: 1447
Type strain: 
Species: Geobacillus subterraneus
Strain Designation: 34, R-35641
Strain history: <- T.N. Nazina; 34
NCBI tax ID(s): 129338 (species)
version 8.1 (current version)
General
@ref: 5084
BacDive-ID: 1447
DSM-Number: 13552
keywords: 16S sequence, Bacteria, aerobe, thermophilic
description: Geobacillus subterraneus 34 is an aerobe, thermophilic bacterium that was isolated from formation water of the Liaohe oil field.
NCBI tax id
- NCBI tax id: 129338
- Matching level: species
strain history
- @ref: 5084
- history: <- T.N. Nazina; 34
doi: 10.13145/bacdive1447.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Geobacillus
- species: Geobacillus subterraneus
- full scientific name: Geobacillus subterraneus Nazina et al. 2001
@ref: 5084
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Geobacillus
species: Geobacillus subterraneus subsp. subterraneus
full scientific name: Geobacillus subterraneus subsp. subterraneus (Nazina et al. 2001) Poli et al. 2013
strain designation: 34, R-35641
type strain: yes
Culture and growth conditions
culture medium
- @ref: 5084
- name: NUTRIENT AGAR (DSMZ Medium 1)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1
- composition: Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 5084 | positive | growth | 55 | thermophilic |
| 60580 | positive | growth | 56 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 60580
- oxygen tolerance: aerobe
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 5084 | formation water of the Liaohe oil field | Liaoring province | China | CHN | Asia |
| 60580 | Formation water | Liaoring province,Liaohe oil field | China | CHN | Asia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Industrial | #Oil reservoir |
| #Environmental | #Aquatic |
taxonmaps
- @ref: 69479
- File name: preview.99_272.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_79;96_191;97_207;98_231;99_272&stattab=map
- Last taxonomy: Geobacillus
- 16S sequence: FN428689
- Sequence Identity:
- Total samples: 8290
- soil counts: 2618
- aquatic counts: 1277
- animal counts: 3638
- plant counts: 757
Safety information
risk assessment
- @ref: 5084
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Geobacillus subterraneus strain DSM 13552 16S-23S ribosomal RNA intergenic spacer and tRNA-Ile gene, partial sequence; and tRNA-Ala gene, complete sequence | EU157950 | 293 | ena | 129338 |
| 20218 | Geobacillus subterraneus isolate Gs2 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | EU723634 | 897 | ena | 129338 |
| 20218 | Geobacillus subterraneus isolate Gs5 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | EU723635 | 623 | ena | 129338 |
| 20218 | Geobacillus subterraneus isolate Gs10 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | EU723636 | 787 | ena | 129338 |
| 5084 | Geobacillus subterraneus partial 16S rRNA gene, strain R-35641 | FN428689 | 1510 | ena | 129338 |
GC content
- @ref: 5084
- GC-content: 52.3
External links
@ref: 5084
culture collection no.: DSM 13552, AS 12673, VKM B-2226, CCUG 55420
straininfo link
- @ref: 71097
- straininfo: 49487
literature
- topic: Enzymology
- Pubmed-ID: 32600313
- title: Functional expression of a novel methanol-stable esterase from Geobacillus subterraneus DSM13552 for biocatalytic synthesis of cinnamyl acetate in a solvent-free system.
- authors: Cai X, Lin L, Shen Y, Wei W, Wei DZ
- journal: BMC Biotechnol
- DOI: 10.1186/s12896-020-00622-1
- year: 2020
- mesh: Bacterial Proteins/genetics, Biocatalysis, Cinnamates/*chemistry, Cloning, Molecular, Enzyme Stability, Escherichia coli/genetics, Esterases/genetics/*metabolism, Geobacillus/genetics/*metabolism, Hydrogen-Ion Concentration, Kinetics, Lipase/metabolism, Methanol, Recombinant Proteins/genetics, Solvents/*chemistry, Substrate Specificity, Temperature
- topic2: Metabolism
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 5084 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13552) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-13552 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 60580 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 55420) | https://www.ccug.se/strain?id=55420 | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 71097 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49487.1 | StrainInfo: A central database for resolving microbial strain identifiers |