Strain identifier

BacDive ID: 14423

Type strain: Yes

Species: Macrococcus brunensis

Strain history: CIP <- 2003, CCM

NCBI tax ID(s): 198483 (species)

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General

@ref: 8071

BacDive-ID: 14423

DSM-Number: 19358

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive

description: Macrococcus brunensis DSM 19358 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from llama skin.

NCBI tax id

  • NCBI tax id: 198483
  • Matching level: species

strain history

@refhistory
8071<- CCM, CCM 4811
339042003, CCM
117063CIP <- 2003, CCM

doi: 10.13145/bacdive14423.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Macrococcus
  • species: Macrococcus brunensis
  • full scientific name: Macrococcus brunensis Mannerová et al. 2003

@ref: 8071

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Macrococcus

species: Macrococcus brunensis

full scientific name: Macrococcus brunensis Mannerová et al. 2003

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
117063positivecoccus-shapedno

colony morphology

  • @ref: 117063

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8071TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
33904MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
117063CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29
117063CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
8071positivegrowth35mesophilic
33904positivegrowth37mesophilic
57575positivegrowth30-37mesophilic
117063positivegrowth22-37
117063nogrowth10psychrophilic
117063nogrowth41thermophilic
117063nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
57575aerobe
117063facultative anaerobe

spore formation

@refspore formationconfidence
69481no97
69480no99.072

halophily

@refsaltgrowthtested relationconcentration
117063NaClpositivegrowth0-4 %
117063NaClnogrowth6 %
117063NaClnogrowth8 %
117063NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
117063606565hippurate+hydrolysis
11706317632nitrate+reduction
11706316301nitrite-reduction
6837516199urea-hydrolysis
6837529016arginine-hydrolysis
6837518257ornithine-degradation
683754853esculin-hydrolysis
6837517634D-glucose-fermentation
6837516024D-mannose-fermentation
6837517306maltose-fermentation
6837517716lactose-fermentation
6837527082trehalose-fermentation
6837516899D-mannitol-fermentation
6837516634raffinose-fermentation
6837516988D-ribose-fermentation
6837517057cellobiose-fermentation
6837517632nitrate+reduction
6837517992sucrose-fermentation
6837559640N-acetylglucosamine-fermentation
6837532528turanose-fermentation
6837522599arabinose-fermentation

antibiotic resistance

  • @ref: 68375
  • ChEBI: 28368
  • metabolite: novobiocin
  • is antibiotic: yes
  • is sensitive: yes
  • sensitivity conc.: 1.8 µg

metabolite production

@refChebi-IDmetaboliteproduction
6837515688acetoinno
11706335581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6837515688acetoin-
11706315688acetoin-

enzymes

@refvalueactivityec
68375beta-glucuronidase-3.2.1.31
68375pyrrolidonyl arylamidase-3.4.19.3
68375alkaline phosphatase+3.1.3.1
68375L-arginine arylamidase-
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase-3.5.3.6
68375urease-3.5.1.5
117063oxidase+
117063beta-galactosidase-3.2.1.23
117063alcohol dehydrogenase-1.1.1.1
117063gelatinase+/-
117063amylase-
117063DNase-
117063caseinase+3.4.21.50
117063catalase+1.11.1.6
117063coagulase-
117063tween esterase-
117063gamma-glutamyltransferase-2.3.2.2
117063lecithinase-
117063lipase-
117063lysine decarboxylase-4.1.1.18
117063ornithine decarboxylase-4.1.1.17
117063phenylalanine ammonia-lyase-4.3.1.24
117063protease+
117063urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117063-++-------++--------

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
8071-----+/---------+---+-------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
8071llama skinBrno, Zoological GardenCzech RepublicCZEEurope
57575Llama skin (Lama glama)Brno,Brno ZooCzech RepublicCZEEurope
117063Skin of llamas, Lama glama L.BrnoCzech RepublicCZEEurope1997

isolation source categories

Cat1Cat2Cat3
#Host#Mammals
#Host Body-Site#Organ#Skin, Nail, Hair

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
80711Risk group (German classification)
1170631Risk group (French classification)

Sequence information

16S sequences

  • @ref: 8071
  • description: Macrococcus brunensis 16S ribosomal RNA gene, complete sequence
  • accession: AY119686
  • length: 1546
  • database: ena
  • NCBI tax ID: 198483

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Macrococcus brunensis CCM4811GCA_004359615contigncbi198483
66792Macrococcus brunensis strain CCM4811198483.3wgspatric198483
66792Macrococcus brunensis CCM48112898327042draftimg198483

GC content

  • @ref: 8071
  • GC-content: 41

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno97no
motileno89.921no
gram-positiveyes95.003no
anaerobicno98.605yes
aerobicyes90.687yes
halophileyes90.69no
spore-formingno78.971no
glucose-utilyes87.752no
flagellatedno92.91no
thermophileno99.642yes
glucose-fermentno79.347yes

External links

@ref: 8071

culture collection no.: DSM 19358, CCM 4811, CCUG 47200, CIP 108167, LMG 21712

straininfo link

  • @ref: 83567
  • straininfo: 87783

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny13130064Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov. and Macrococcus lamae sp. nov., from the skin of llamas.Mannerova S, Pantucek R, Doskar J, Svec P, Snauwaert C, Vancanneyt M, Swings J, Sedlacek IInt J Syst Evol Microbiol10.1099/ijs.0.02683-02003Animals, Base Composition, Base Sequence, Camelids, New World/*microbiology, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Fatty Acids/analysis, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Skin/microbiology, Staphylococcaceae/*classification/genetics/*isolation & purification/metabolismGenetics
Phylogeny27272908Abyssicoccus albus gen. nov., sp. nov., a novel member of the family Staphylococcaceae isolated from marine sediment of the Indian Ocean.Jiang Z, Yuan CG, Xiao M, Tian XP, Khan IU, Kim CJ, Zhi XY, Li WJAntonie Van Leeuwenhoek10.1007/s10482-016-0717-22016Cell Wall/chemistry, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Geologic Sediments/*microbiology, Indian Ocean, Peptidoglycan/metabolism, Phospholipids/metabolism, Phylogeny, Seawater/microbiology, Sequence Analysis, DNA, Staphylococcaceae/*classification/genetics/*isolation & purification/metabolism, Vitamin K 2/metabolismMetabolism
Phylogeny28086066Description of Auricoccus indicus gen. nov., sp. nov., isolated from skin of human ear.Prakash O, Muduli S, Kumar R, Kumari C, Nimonkar Y, Shouche YS, Sharma RInt J Syst Evol Microbiol10.1099/ijsem.0.0017872017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Ear/*microbiology, Fatty Acids/chemistry, Gram-Positive Asporogenous Rods/*classification/genetics/isolation & purification, Humans, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Skin/*microbiologyPathogenicity
Genetics31072882Draft Genome Sequences of the Type Strains of Six Macrococcus Species.Mazhar S, Altermann E, Hill C, McAuliffe OMicrobiol Resour Announc10.1128/MRA.00344-192019

Reference

@idauthorscataloguedoi/urltitle
8071Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19358)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19358
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33904Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5759
57575Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47200)https://www.ccug.se/strain?id=47200
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68375Automatically annotated from API ID32STA
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83567Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID87783.1StrainInfo: A central database for resolving microbial strain identifiers
117063Curators of the CIPCollection of Institut Pasteur (CIP 108167)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108167