Name: Macrococcus brunensis DSM 19358
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14423

Type strain:

Species: Macrococcus brunensis

Culture col. no.: DSM 19358, CCM 4811, CCUG 47200, CIP 108167, LMG 21712

Strain history: CIP <- 2003, CCM

NCBI tax ID(s): 198483 (species)

SI-ID 87783

CCM 4811, LMG 21712, CCUG 47200, CIP 108167, DSM 19358

Section

Macrococcus brunensis DSM 19358 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from llama skin.

Gram-positive
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Staphylococcaceae*
	Genus	*Macrococcus*
	Species	**Macrococcus brunensis**
	Full Scientific Name (LPSN)	Macrococcus brunensis Mannerová et al. 2003

Information on morphological and physiological properties Morphology

[Ref.: #117063]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #117063]	Cell shape	coccus-shaped

[Ref.: #117063]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8071]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #8071]

Culture medium growth

[Ref.: #8071]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8071]

Culture medium composition

expand / minimize

[Ref.: #33904]

Culture medium

MEDIUM 29- Brain heart agar

[Ref.: #33904]

Culture medium growth

[Ref.: #33904]

Culture medium composition

expand / minimize

[Ref.: #117063]

Culture medium

CIP Medium 29

[Ref.: #117063]

Culture medium growth

[Ref.: #117063]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #117063]

Culture medium

CIP Medium 72

[Ref.: #117063]

Culture medium growth

[Ref.: #117063]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #8071]		growth	35 °C
[Ref.: #33904]		growth	37 °C
[Ref.: #57575]		growth	30-37 °C
[Ref.: #117063]		growth	22-37 °C
[Ref.: #117063]	no	growth	10 °C
[Ref.: #117063]	no	growth	41 °C
[Ref.: #117063]	no	growth	45 °C

[Ref.: #8071]	Temperature range	mesophilic
[Ref.: #33904]	Temperature range	mesophilic
[Ref.: #57575]	Temperature range	mesophilic
[Ref.: #117063]	Temperature range	psychrophilic
[Ref.: #117063]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #57575]	Oxygen tolerance	aerobe
[Ref.: #117063]	Oxygen tolerance	facultative anaerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %97
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.072

	Salt	Tested relation		Salt conc.
[Ref.: #117063]	NaCl		growth	0-4	%
[Ref.: #117063]	NaCl	no	growth	6	%
[Ref.: #117063]	NaCl	no	growth	8	%
[Ref.: #117063]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68375]	arabinose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	arginine ChEBI	-	hydrolysis	* from API ID32STA
[Ref.: #68375]	cellobiose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	D-glucose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	D-mannitol ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	D-mannose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	D-ribose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	esculin ChEBI	-	hydrolysis	* from API ID32STA
[Ref.: #117063]	hippurate ChEBI	+	hydrolysis
[Ref.: #68375]	lactose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	maltose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	N-acetylglucosamine ChEBI	-	fermentation	* from API ID32STA
[Ref.: #117063]	nitrate ChEBI	+	reduction
[Ref.: #68375]	nitrate ChEBI	+	reduction	* from API ID32STA
[Ref.: #117063]	nitrite ChEBI	-	reduction
[Ref.: #68375]	ornithine ChEBI	-	degradation	* from API ID32STA
[Ref.: #68375]	raffinose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	sucrose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	trehalose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	turanose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	urea ChEBI	-	hydrolysis	* from API ID32STA
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #68375]		novobiocin ChEBI	yes	1.8	µg	* from API ID32STA

	Metabolite production	Metabolite	Production
[Ref.: #117063]		indole ChEBI	no
[Ref.: #68375]		acetoin ChEBI	no	* from API ID32STA

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #117063]		acetoin ChEBI	-
[Ref.: #68375]		acetoin ChEBI	-	* from API ID32STA

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #117063]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68375]	alkaline phosphatase	+	3.1.3.1	* from API ID32STA, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #117063]	amylase	-
[Ref.: #68375]	arginine dihydrolase	-	3.5.3.6	* from API ID32STA
[Ref.: #117063]	beta-galactosidase	-	3.2.1.23
[Ref.: #68375]	beta-galactosidase	-	3.2.1.23	* from API ID32STA, API zym
[Ref.: #68375]	beta-glucosidase	-	3.2.1.21	* from API ID32STA, API zym
[Ref.: #68375]	beta-glucuronidase	-	3.2.1.31	* from API ID32STA, API zym
[Ref.: #117063]	caseinase	+	3.4.21.50
[Ref.: #117063]	catalase	+	1.11.1.6
[Ref.: #117063]	coagulase	-
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #117063]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #117063]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #117063]	gelatinase	+/-
[Ref.: #68375]	L-arginine arylamidase	-		* from API ID32STA
[Ref.: #117063]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #117063]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #117063]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #117063]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68375]	ornithine decarboxylase	-	4.1.1.17	* from API ID32STA
[Ref.: #117063]	oxidase	+
[Ref.: #117063]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #117063]	protease	+
[Ref.: #68375]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API ID32STA
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #117063]	tween esterase	-
[Ref.: #117063]	urease	-	3.5.1.5
[Ref.: #68375]	urease	-	3.5.1.5	* from API ID32STA
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® ID32STA:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	Ornithine decarboxylaseODC	Esculin hydrolysisESC	Fermentation of D-glucoseGLU	Fermentation of D-fructoseFRU	Fermentation of D-mannoseMNE	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of trehaloseTRE	Fermentation of D-mannitolMAN	Fermentation of raffinoseRAF	Fermentation of D-riboseRIB	Fermentation of cellobioseCEL	Reduction of nitrateNIT	Acetoin production (Voges Proskauer test)VP	beta-Galactosidasebeta GAL	L-arginine arylamidaseArgA	Alkaline phosphatasePAL	Pyrrolidonyl arylamidasePyrA	Novobiocin resistanceNOVO	Fermentation of sucroseSAC	Fermentation of N-acetyl-glucosamineNAG	Fermentation of turanoseTUR	Fermentation of arabinoseARA	beta-Glucuronidasebeta GUR
[Ref.: #8071]	6152	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	+	-	-	-	+	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #117063]	10946	-	+	+	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8071]	Sample type/isolated from	llama skin
[Ref.: #8071]	Geographic location (country and/or sea, region)	Brno, Zoological Garden
[Ref.: #8071]	Country	Czech Republic
[Ref.: #8071]	Country ISO 3 Code	CZE
[Ref.: #8071]	Continent	Europe

[Ref.: #57575]	Sample type/isolated from	Llama skin (Lama glama)
[Ref.: #57575]	Geographic location (country and/or sea, region)	Brno,Brno Zoo
[Ref.: #57575]	Country	Czech Republic
[Ref.: #57575]	Country ISO 3 Code	CZE
[Ref.: #57575]	Continent	Europe

[Ref.: #117063]	Sample type/isolated from	Skin of llamas, Lama glama L.
[Ref.: #117063]	Geographic location (country and/or sea, region)	Brno
[Ref.: #117063]	Country	Czech Republic
[Ref.: #117063]	Country ISO 3 Code	CZE
[Ref.: #117063]	Continent	Europe
[Ref.: #117063]	Isolation date	1997
* marker position based on {}

Isolation sources categories

#Host	#Mammals	-
#Host Body-Site	#Organ	#Skin, Nail, Hair

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8071]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #117063]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8071]	Macrococcus brunensis 16S ribosomal RNA gene, complete sequence	AY119686	1546	ENA	198483 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Macrococcus brunensis CCM4811	GCA_004359615	contig	GenBank	198483 tax ID	*
[Ref.: #66792]	Macrococcus brunensis strain CCM4811	198483.3	wgs	PATRIC	198483 tax ID	*
[Ref.: #66792]	Macrococcus brunensis CCM4811	2898327042	draft	JGI IMG/M	198483 tax ID	*

[Ref.: #8071]

GC-content

41 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Macrococcus brunensis strain CCM4811 PATRIC: 198483.3
[Ref.: #69481]	spore-forming	no	97	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Macrococcus brunensis strain CCM4811 PATRIC: 198483.3
[Ref.: #69480]	motile	no	89.921	no
[Ref.: #69480]	gram-positive	yes	95.003	no
[Ref.: #69480]	anaerobic	no	98.605	yes
[Ref.: #69480]	aerobic	yes	90.687	yes
[Ref.: #69480]	halophile	yes	90.69	no
[Ref.: #69480]	spore-forming	no	78.971	no
[Ref.: #69480]	glucose-util	yes	87.752	no
[Ref.: #69480]	flagellated	no	92.91	no
[Ref.: #69480]	thermophile	no	99.642	yes
[Ref.: #69480]	glucose-ferment	no	79.347	yes

Availability in culture collections External links

[Ref.: #8071]

Culture collection no.

DSM 19358, CCM 4811, CCUG 47200, CIP 108167, LMG 21712

[Ref.: #83567]

SI-ID 87783

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft Genome Sequences of the Type Strains of Six Macrococcus Species.	Mazhar S, Altermann E, Hill C, McAuliffe O	Microbiol Resour Announc	10.1128/MRA.00344-19	2019	*
Phylogeny	Description of Auricoccus indicus gen. nov., sp. nov., isolated from skin of human ear.	Prakash O, Muduli S, Kumar R, Kumari C, Nimonkar Y, Shouche YS, Sharma R	Int J Syst Evol Microbiol	10.1099/ijsem.0.001787	2017	*
Phylogeny	Abyssicoccus albus gen. nov., sp. nov., a novel member of the family Staphylococcaceae isolated from marine sediment of the Indian Ocean.	Jiang Z, Yuan CG, Xiao M, Tian XP, Khan IU, Kim CJ, Zhi XY, Li WJ	Antonie Van Leeuwenhoek	10.1007/s10482-016-0717-2	2016	*
Phylogeny	Macrococcus brunensis sp. nov., Macrococcus hajekii sp. nov. and Macrococcus lamae sp. nov., from the skin of llamas.	Mannerova S, Pantucek R, Doskar J, Svec P, Snauwaert C, Vancanneyt M, Swings J, Sedlacek I	Int J Syst Evol Microbiol	10.1099/ijs.0.02683-0	2003	*

References

#8071

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19358

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#33904 ; Curators of the CIP;
#57575 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 47200

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68375

Automatically annotated from API ID32STA .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83567

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID87783.1 )

#117063 Collection of Institut Pasteur ; Curators of the CIP; CIP 108167
* These data were automatically processed and therefore are not curated

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Macrococcus brunensis strain CCM4811

Macrococcus brunensis strain CCM4811

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