Strain identifier
BacDive ID: 14374
Type strain: 
Species: Spiroplasma helicoides
Strain Designation: TABS-2
Strain history: <- J. K. Davis, Dept. Agronomy, Purdue Univ., West Lafayette, USA <- J. G. Tully <- R. F. Whitcomb, USDA
NCBI tax ID(s): 216938 (species)
version 8.1 (current version)
General
@ref: 16392
BacDive-ID: 14374
DSM-Number: 22551
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive
description: Spiroplasma helicoides TABS-2 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from gut of a horsefly .
NCBI tax id
- NCBI tax id: 216938
- Matching level: species
strain history
- @ref: 16392
- history: <- J. K. Davis, Dept. Agronomy, Purdue Univ., West Lafayette, USA <- J. G. Tully <- R. F. Whitcomb, USDA
doi: 10.13145/bacdive14374.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/mycoplasmatota
- domain: Bacteria
- phylum: Mycoplasmatota
- class: Mollicutes
- order: Mycoplasmatales
- family: Spiroplasmataceae
- genus: Spiroplasma
- species: Spiroplasma helicoides
- full scientific name: Spiroplasma helicoides Whitcomb et al. 1997
@ref: 16392
domain: Bacteria
phylum: Tenericutes
class: Mollicutes
order: Entomoplasmatales
family: Spiroplasmataceae
genus: Spiroplasma
species: Spiroplasma helicoides
full scientific name: Spiroplasma helicoides Whitcomb et al. 1997
strain designation: TABS-2
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 99.719
Culture and growth conditions
culture medium
- @ref: 16392
- name: SP4-Z MEDIUM (DSMZ Medium 1076b)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1076b
- composition: Name: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water
culture temp
- @ref: 16392
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 16392
- oxygen tolerance: microaerophile
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.999
Isolation, sampling and environmental information
isolation
- @ref: 16392
- sample type: gut of a horsefly (Tabanus abactor)
- host species: Tabanus abactor
- geographic location: Oklahoma, Carter County, near Ardmore
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Arthropoda | #Insecta |
| #Host Body-Site | #Gastrointestinal tract | #Stomach |
taxonmaps
- @ref: 69479
- File name: preview.99_4928.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_109;96_1346;97_1598;98_3691;99_4928&stattab=map
- Last taxonomy: Spiroplasma
- 16S sequence: AY189132
- Sequence Identity:
- Total samples: 57
- soil counts: 1
- aquatic counts: 2
- animal counts: 54
Safety information
risk assessment
- @ref: 16392
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 16392
- description: Spiroplasma helicoides 16S ribosomal RNA gene, partial sequence
- accession: AY189132
- length: 1505
- database: ena
- NCBI tax ID: 216938
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Spiroplasma helicoides TABS-2 | GCA_001715535 | complete | ncbi | 216938 |
| 66792 | Spiroplasma helicoides strain TABS-2 | 216938.3 | complete | patric | 216938 |
| 66792 | Spiroplasma helicoides TABS-2 | 2791355054 | complete | img | 216938 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 62 | no |
| flagellated | no | 98.395 | no |
| gram-positive | yes | 69.7 | no |
| anaerobic | no | 93.869 | no |
| aerobic | no | 97.868 | yes |
| halophile | yes | 59.122 | no |
| spore-forming | no | 97.634 | no |
| glucose-util | yes | 92.381 | no |
| thermophile | no | 93.598 | no |
| motile | no | 93.81 | no |
| glucose-ferment | yes | 79.888 | no |
External links
@ref: 16392
culture collection no.: DSM 22551, ATCC 51746
straininfo link
- @ref: 83517
- straininfo: 43156
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 9542070 | Revised group classification of the genus Spiroplasma. | Williamson DL, Whitcomb RF, Tully JG, Gasparich GE, Rose DL, Carle P, Bove JM, Hackett KJ, Adams JR, Henegar RB, Konai M, Chastel C, French FE | Int J Syst Bacteriol | 10.1099/00207713-48-1-1 | 1998 | Animals, Antibodies, Bacterial, Cell Membrane/ultrastructure, Classification, DNA, Bacterial/analysis, Glucose/metabolism, Insecta/*microbiology, Microscopy, Electron, Plants/*microbiology, Serologic Tests, Spiroplasma/chemistry/*classification/immunology | Metabolism |
| Genetics | 27795290 | Complete Genome Sequence of Spiroplasma helicoides TABS-2T (DSM 22551), a Bacterium Isolated from a Horsefly (Tabanus abactor). | Shen WY, Lo WS, Lai YC, Kuo CH | Genome Announc | 10.1128/genomeA.01201-16 | 2016 | Phylogeny |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 16392 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22551) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-22551 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 83517 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID43156.1 | StrainInfo: A central database for resolving microbial strain identifiers |