Name: Spiroplasma helicoides TABS-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14374

Type strain:

Species: Spiroplasma helicoides

Strain Designation: TABS-2

Culture col. no.: DSM 22551, ATCC 51746

Strain history: <- J. K. Davis, Dept. Agronomy, Purdue Univ., West Lafayette, USA <- J. G. Tully <- R. F. Whitcomb, USDA

NCBI tax ID(s): 216938 (species)

SI-ID 43156

ATCC 51746

Section

Spiroplasma helicoides TABS-2 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from gut of a horsefly .

Gram-positive
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Mycoplasmatota*
	Class	*Mollicutes*
	Order	*Mycoplasmatales*
	Family	*Spiroplasmataceae*
	Genus	*Spiroplasma*
	Species	**Spiroplasma helicoides**
	Full Scientific Name (LPSN)	Spiroplasma helicoides Whitcomb et al. 1997

Information on morphological and physiological properties Morphology

[Ref.: #69480]

Gram stain

positive Confidence in %99.719

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16392]

Culture medium

SP4-Z MEDIUM (DSMZ Medium 1076b)

[Ref.: #16392]

Culture medium growth

[Ref.: #16392]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16392]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #16392]			growth	30 °C

[Ref.: #16392]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16392]

Oxygen tolerance

microaerophile

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.999

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	palmitate biosynthesis	77.27	17 of 22
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	ppGpp biosynthesis	75	3 of 4
[Ref.: #66794]	degradation of sugar alcohols	75	12 of 16
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	UDP-GlcNAc biosynthesis	66.67	2 of 3
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	pentose phosphate pathway	63.64	7 of 11
[Ref.: #66794]	ketogluconate metabolism	62.5	5 of 8
[Ref.: #66794]	gluconeogenesis	62.5	5 of 8
[Ref.: #66794]	glycine betaine biosynthesis	60	3 of 5
[Ref.: #66794]	aspartate and asparagine metabolism	55.56	5 of 9
[Ref.: #66794]	Entner Doudoroff pathway	50	5 of 10
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	C4 and CAM-carbon fixation	50	4 of 8
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	sulfopterin metabolism	50	2 of 4
[Ref.: #66794]	adipate degradation	50	1 of 2
[Ref.: #66794]	ribulose monophosphate pathway	50	1 of 2
[Ref.: #66794]	purine metabolism	50	47 of 94
[Ref.: #66794]	pyrimidine metabolism	48.89	22 of 45
[Ref.: #66794]	phenylalanine metabolism	46.15	6 of 13
[Ref.: #66794]	oxidative phosphorylation	46.15	42 of 91
[Ref.: #66794]	metabolism of disaccharids	45.45	5 of 11
[Ref.: #66794]	d-xylose degradation	45.45	5 of 11
[Ref.: #66794]	serine metabolism	44.44	4 of 9
[Ref.: #66794]	NAD metabolism	44.44	8 of 18
[Ref.: #66794]	d-mannose degradation	44.44	4 of 9
[Ref.: #66794]	propanol degradation	42.86	3 of 7
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	glycogen metabolism	40	2 of 5
[Ref.: #66794]	methylglyoxal degradation	40	2 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	40	2 of 5
[Ref.: #66794]	lipoate biosynthesis	40	2 of 5
[Ref.: #66794]	glutamate and glutamine metabolism	39.29	11 of 28
[Ref.: #66794]	vitamin B1 metabolism	38.46	5 of 13
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	37.5	3 of 8
[Ref.: #66794]	degradation of pentoses	35.71	10 of 28
[Ref.: #66794]	lipid metabolism	35.48	11 of 31
[Ref.: #66794]	isoprenoid biosynthesis	34.62	9 of 26
[Ref.: #66794]	methionine metabolism	34.62	9 of 26
[Ref.: #66794]	glycolate and glyoxylate degradation	33.33	2 of 6
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	octane oxidation	33.33	1 of 3
[Ref.: #66794]	non-pathway related	31.58	12 of 38
[Ref.: #66794]	reductive acetyl coenzyme A pathway	28.57	2 of 7
[Ref.: #66794]	glutathione metabolism	28.57	4 of 14
[Ref.: #66794]	tyrosine metabolism	28.57	4 of 14
[Ref.: #66794]	tetrahydrofolate metabolism	28.57	4 of 14
[Ref.: #66794]	proline metabolism	27.27	3 of 11
[Ref.: #66794]	flavin biosynthesis	26.67	4 of 15
[Ref.: #66794]	tryptophan metabolism	26.32	10 of 38
[Ref.: #66794]	glycogen biosynthesis	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	androgen and estrogen metabolism	25	4 of 16
[Ref.: #66794]	alanine metabolism	24.14	7 of 29
[Ref.: #66794]	leucine metabolism	23.08	3 of 13
[Ref.: #66794]	ascorbate metabolism	22.73	5 of 22
[Ref.: #66794]	CO2 fixation in Crenarchaeota	22.22	2 of 9
[Ref.: #66794]	cysteine metabolism	22.22	4 of 18
[Ref.: #66794]	lipid A biosynthesis	22.22	2 of 9
[Ref.: #66794]	valine metabolism	22.22	2 of 9
[Ref.: #66794]	citric acid cycle	21.43	3 of 14
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16392]	Sample type/isolated from	gut of a horsefly (Tabanus abactor)
[Ref.: #16392]	Host species	Tabanus abactor
[Ref.: #16392]	Geographic location (country and/or sea, region)	Oklahoma, Carter County, near Ardmore
[Ref.: #16392]	Country	USA
[Ref.: #16392]	Country ISO 3 Code	USA
[Ref.: #16392]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	#Stomach

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY189132 (>99% sequence identity) for Spiroplasma from Microbeatlas

Aquatic Samples	2
Soil Samples	1
Animal Samples	54
Plant Samples
Total Samples	57

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16392]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16392]	Spiroplasma helicoides 16S ribosomal RNA gene, partial sequence	AY189132	1505	ENA	216938 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Spiroplasma helicoides TABS-2	GCA_001715535	complete	GenBank	216938 tax ID	*
[Ref.: #66792]	Spiroplasma helicoides strain TABS-2	216938.3	complete	PATRIC	216938 tax ID	*
[Ref.: #66792]	Spiroplasma helicoides TABS-2	2791355054	complete	JGI IMG/M	216938 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Spiroplasma helicoides TABS-2 NCBI: GCA_001715535.1
[Ref.: #69481]	spore-forming	yes	62	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Spiroplasma helicoides TABS-2 NCBI: GCA_001715535.1
[Ref.: #69480]	flagellated	no	98.395	no
[Ref.: #69480]	gram-positive	yes	69.7	no
[Ref.: #69480]	anaerobic	no	93.869	no
[Ref.: #69480]	aerobic	no	97.868	yes
[Ref.: #69480]	halophile	yes	59.122	no
[Ref.: #69480]	spore-forming	no	97.634	no
[Ref.: #69480]	glucose-util	yes	92.381	no
[Ref.: #69480]	thermophile	no	93.598	no
[Ref.: #69480]	motile	no	93.81	no
[Ref.: #69480]	glucose-ferment	yes	79.888	no

Availability in culture collections External links

[Ref.: #16392]

Culture collection no.

DSM 22551, ATCC 51746

[Ref.: #83517]

SI-ID 43156

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of Spiroplasma helicoides TABS-2T (DSM 22551), a Bacterium Isolated from a Horsefly (Tabanus abactor).	Shen WY, Lo WS, Lai YC, Kuo CH	Genome Announc	10.1128/genomeA.01201-16	2016	*
Phylogeny	Revised group classification of the genus Spiroplasma.	Williamson DL, Whitcomb RF, Tully JG, Gasparich GE, Rose DL, Carle P, Bove JM, Hackett KJ, Adams JR, Henegar RB, Konai M, Chastel C, French FE	Int J Syst Bacteriol	10.1099/00207713-48-1-1	1998	*

References

#16392

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22551

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83517

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID43156.1 )

* These data were automatically processed and therefore are not curated

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