Strain identifier

BacDive ID: 14358

Type strain: Yes

Species: Spiroplasma mirum

Strain Designation: SMCA

Strain history: <- J. K. Davis, Dept. Agronomy, Purdue Univ., West Lafayette, USA <- J. G. Tully <- F. E. Clark

NCBI tax ID(s): 838561 (strain), 2144 (species)

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General

@ref: 16394

BacDive-ID: 14358

DSM-Number: 22553

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, animal pathogen

description: Spiroplasma mirum SMCA is a microaerophile, mesophilic, Gram-positive animal pathogen that was isolated from rabbit tick Haemaphysalis leporipalustris.

NCBI tax id

NCBI tax idMatching level
2144species
838561strain

strain history

  • @ref: 16394
  • history: <- J. K. Davis, Dept. Agronomy, Purdue Univ., West Lafayette, USA <- J. G. Tully <- F. E. Clark

doi: 10.13145/bacdive14358.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/mycoplasmatota
  • domain: Bacteria
  • phylum: Mycoplasmatota
  • class: Mollicutes
  • order: Mycoplasmatales
  • family: Spiroplasmataceae
  • genus: Spiroplasma
  • species: Spiroplasma mirum
  • full scientific name: Spiroplasma mirum Tully et al. 1982

@ref: 16394

domain: Bacteria

phylum: Tenericutes

class: Mollicutes

order: Entomoplasmatales

family: Spiroplasmataceae

genus: Spiroplasma

species: Spiroplasma mirum

full scientific name: Spiroplasma mirum Tully et al. 1982

strain designation: SMCA

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 99.546

Culture and growth conditions

culture medium

  • @ref: 16394
  • name: SP4-Z MEDIUM (DSMZ Medium 1076b)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1076b
  • composition: Name: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water

culture temp

  • @ref: 16394
  • growth: positive
  • type: growth
  • temperature: 30
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 16394
  • oxygen tolerance: microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.999

Isolation, sampling and environmental information

isolation

  • @ref: 16394
  • sample type: rabbit tick Haemaphysalis leporipalustris
  • host species: Haemaphysalis leporipalustris

isolation source categories

Cat1Cat2Cat3
#Host#Arthropoda#Tick
#Host#Mammals#Leporidae (Rabbit/Hare)

taxonmaps

  • @ref: 69479
  • File name: preview.99_307.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15899;96_212;97_231;98_260;99_307&stattab=map
  • Last taxonomy: Spiroplasma
  • 16S sequence: JX898939
  • Sequence Identity:
  • Total samples: 198
  • soil counts: 34
  • aquatic counts: 45
  • animal counts: 110
  • plant counts: 9

Safety information

risk assessment

  • @ref: 16394
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Spiroplasma mirum strain ATCC 29335 16S ribosomal RNA gene, partial sequenceJX8989391466ena2144
16394Spiroplasma mirum strain SMCA 16S ribosomal RNA small subunit gene, partial sequenceM246621467ena838561

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Spiroplasma mirum ATCC 29335GCA_000517365completencbi838561
66792Spiroplasma mirum ATCC 29335 SMCAGCA_000565195completencbi838561
66792Spiroplasma mirum ATCC 29335838561.4completepatric838561
66792Spiroplasma mirum ATCC 29335 [PRJNA217816]838561.3completepatric838561
66792Spiroplasma mirum ATCC 293352558860181completeimg838561
66792Spiroplasma mirum SMCA2558860251completeimg838561

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno88.71no
flagellatedno98.354no
gram-positiveyes68.833no
anaerobicno94.578yes
halophileyes64.827no
spore-formingno98.703no
thermophileno90.117no
glucose-utilyes89.193no
aerobicno98.137no
glucose-fermentyes76.725no

External links

@ref: 16394

culture collection no.: DSM 22553, ATCC 29335, CIP 103925

straininfo link

  • @ref: 83501
  • straininfo: 39477

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Biotechnology8167436Production and characterization of a polyclonal antiserum against Spiroplasma mirum (ATCC 29335).Tenckhoff B, Kolmel HW, Wolf V, Lange RZentralbl Bakteriol10.1016/s0934-8840(11)80605-51994Animals, Antibodies, Bacterial/biosynthesis/*immunology, Gram-Positive Bacterial Infections/*diagnosis, Immune Sera, Spiroplasma/*immunology, Ticks/*microbiology
Phylogeny20418415Spiroplasma eriocheiris sp. nov., associated with mortality in the Chinese mitten crab, Eriocheir sinensis.Wang W, Gu W, Gasparich GE, Bi K, Ou J, Meng Q, Liang T, Feng Q, Zhang J, Zhang YInt J Syst Evol Microbiol10.1099/ijs.0.020529-02010Animals, Antibodies, Bacterial/immunology, Bacterial Typing Techniques, Base Composition, Brachyura/*microbiology, China, Cluster Analysis, Cross Reactions, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Hemolymph/microbiology, Locomotion, Microscopy, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spiroplasma/*classification/genetics/*isolation & purification/physiologyGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16394Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22553)https://www.dsmz.de/collection/catalogue/details/culture/DSM-22553
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
83501Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID39477.1StrainInfo: A central database for resolving microbial strain identifiers