Name: Spiroplasma mirum SMCA
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14358

Type strain:

Species: Spiroplasma mirum

Strain Designation: SMCA

Culture col. no.: DSM 22553, ATCC 29335, CIP 103925

Strain history: <- J. K. Davis, Dept. Agronomy, Purdue Univ., West Lafayette, USA <- J. G. Tully <- F. E. Clark

NCBI tax ID(s): 838561 (strain), 2144 (species)

SI-ID 39477

ATCC 29335, CIP 103925, DSM 22553

Section

Spiroplasma mirum SMCA is a microaerophile, mesophilic, Gram-positive animal pathogen that was isolated from rabbit tick Haemaphysalis leporipalustris.

Gram-positive
animal pathogen
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Mycoplasmatota*
	Class	*Mollicutes*
	Order	*Mycoplasmatales*
	Family	*Spiroplasmataceae*
	Genus	*Spiroplasma*
	Species	**Spiroplasma mirum**
	Full Scientific Name (LPSN)	Spiroplasma mirum Tully et al. 1982

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %99.546

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16394]

Culture medium

SP4-Z MEDIUM (DSMZ Medium 1076b)

[Ref.: #16394]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #16394]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #16394]			growth	30 °C

[Ref.: #16394]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16394]

Oxygen tolerance

microaerophile

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.999

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	palmitate biosynthesis	77.27	17 of 22
[Ref.: #66794]	ppGpp biosynthesis	75	3 of 4
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	UDP-GlcNAc biosynthesis	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	glycolysis	64.71	11 of 17
[Ref.: #66794]	photosynthesis	64.29	9 of 14
[Ref.: #66794]	pyrimidine metabolism	51.11	23 of 45
[Ref.: #66794]	suberin monomers biosynthesis	50	1 of 2
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	acetate fermentation	50	2 of 4
[Ref.: #66794]	NAD metabolism	50	9 of 18
[Ref.: #66794]	sulfopterin metabolism	50	2 of 4
[Ref.: #66794]	ribulose monophosphate pathway	50	1 of 2
[Ref.: #66794]	phenylalanine metabolism	46.15	6 of 13
[Ref.: #66794]	vitamin B1 metabolism	46.15	6 of 13
[Ref.: #66794]	purine metabolism	45.74	43 of 94
[Ref.: #66794]	d-mannose degradation	44.44	4 of 9
[Ref.: #66794]	valine metabolism	44.44	4 of 9
[Ref.: #66794]	propanol degradation	42.86	3 of 7
[Ref.: #66794]	cardiolipin biosynthesis	42.86	3 of 7
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	lipoate biosynthesis	40	2 of 5
[Ref.: #66794]	methylglyoxal degradation	40	2 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	40	2 of 5
[Ref.: #66794]	ketogluconate metabolism	37.5	3 of 8
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	37.5	3 of 8
[Ref.: #66794]	oxidative phosphorylation	37.36	34 of 91
[Ref.: #66794]	pentose phosphate pathway	36.36	4 of 11
[Ref.: #66794]	tetrahydrofolate metabolism	35.71	5 of 14
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	degradation of pentoses	32.14	9 of 28
[Ref.: #66794]	non-pathway related	31.58	12 of 38
[Ref.: #66794]	isoprenoid biosynthesis	30.77	8 of 26
[Ref.: #66794]	methionine metabolism	30.77	8 of 26
[Ref.: #66794]	threonine metabolism	30	3 of 10
[Ref.: #66794]	Entner Doudoroff pathway	30	3 of 10
[Ref.: #66794]	lipid metabolism	29.03	9 of 31
[Ref.: #66794]	reductive acetyl coenzyme A pathway	28.57	2 of 7
[Ref.: #66794]	glutamate and glutamine metabolism	28.57	8 of 28
[Ref.: #66794]	glutathione metabolism	28.57	4 of 14
[Ref.: #66794]	tyrosine metabolism	28.57	4 of 14
[Ref.: #66794]	d-xylose degradation	27.27	3 of 11
[Ref.: #66794]	flavin biosynthesis	26.67	4 of 15
[Ref.: #66794]	isoleucine metabolism	25	2 of 8
[Ref.: #66794]	degradation of sugar alcohols	25	4 of 16
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	25	1 of 4
[Ref.: #66794]	glycogen biosynthesis	25	1 of 4
[Ref.: #66794]	C4 and CAM-carbon fixation	25	2 of 8
[Ref.: #66794]	butanoate fermentation	25	1 of 4
[Ref.: #66794]	alanine metabolism	24.14	7 of 29
[Ref.: #66794]	degradation of sugar acids	24	6 of 25
[Ref.: #66794]	tryptophan metabolism	23.68	9 of 38
[Ref.: #66794]	leucine metabolism	23.08	3 of 13
[Ref.: #66794]	urea cycle	23.08	3 of 13
[Ref.: #66794]	nitrate assimilation	22.22	2 of 9
[Ref.: #66794]	CO2 fixation in Crenarchaeota	22.22	2 of 9
[Ref.: #66794]	lipid A biosynthesis	22.22	2 of 9
[Ref.: #66794]	cysteine metabolism	22.22	4 of 18
[Ref.: #66794]	arginine metabolism	20.83	5 of 24
[Ref.: #66794]	histidine metabolism	20.69	6 of 29
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16394]	Sample type/isolated from	rabbit tick Haemaphysalis leporipalustris
[Ref.: #16394]	Host species	Haemaphysalis leporipalustris
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Tick
#Host	#Mammals	#Leporidae (Rabbit/Hare)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JX898939 (>99% sequence identity) for Spiroplasma from Microbeatlas

Aquatic Samples	45
Soil Samples	34
Animal Samples	110
Plant Samples	9
Total Samples	198

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16394]	Pathogenicity (animal)	yes
[Ref.: #16394]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16394]	Spiroplasma mirum strain SMCA 16S ribosomal RNA small subunit gene, partial sequence	M24662	1467	ENA	838561 tax ID
[Ref.: #20218]	Spiroplasma mirum strain ATCC 29335 16S ribosomal RNA gene, partial sequence	JX898939	1466	ENA	2144 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Spiroplasma mirum ATCC 29335	GCA_000517365	complete	GenBank	838561 tax ID	*
[Ref.: #66792]	Spiroplasma mirum ATCC 29335 SMCA	GCA_000565195	complete	GenBank	838561 tax ID	*
[Ref.: #66792]	Spiroplasma mirum ATCC 29335	838561.4	complete	PATRIC	838561 tax ID	*
[Ref.: #66792]	Spiroplasma mirum ATCC 29335 [PRJNA217816]	838561.3	complete	PATRIC	838561 tax ID	*
[Ref.: #66792]	Spiroplasma mirum ATCC 29335	2558860181	complete	JGI IMG/M	838561 tax ID	*
[Ref.: #66792]	Spiroplasma mirum SMCA	2558860251	complete	JGI IMG/M	838561 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Spiroplasma mirum ATCC 29335 ATCC 29335 NCBI: GCA_000517365.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Spiroplasma mirum ATCC 29335 ATCC 29335 NCBI: GCA_000517365.1
[Ref.: #69480]	motile	no	88.71	no
[Ref.: #69480]	flagellated	no	98.354	no
[Ref.: #69480]	gram-positive	yes	68.833	no
[Ref.: #69480]	anaerobic	no	94.578	yes
[Ref.: #69480]	halophile	yes	64.827	no
[Ref.: #69480]	spore-forming	no	98.703	no
[Ref.: #69480]	thermophile	no	90.117	no
[Ref.: #69480]	glucose-util	yes	89.193	no
[Ref.: #69480]	aerobic	no	98.137	no
[Ref.: #69480]	glucose-ferment	yes	76.725	no

Availability in culture collections External links

[Ref.: #16394]

Culture collection no.

DSM 22553, ATCC 29335, CIP 103925

[Ref.: #83501]

SI-ID 39477

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Spiroplasma eriocheiris sp. nov., associated with mortality in the Chinese mitten crab, Eriocheir sinensis.	Wang W, Gu W, Gasparich GE, Bi K, Ou J, Meng Q, Liang T, Feng Q, Zhang J, Zhang Y	Int J Syst Evol Microbiol	10.1099/ijs.0.020529-0	2010	*
Biotechnology	Production and characterization of a polyclonal antiserum against Spiroplasma mirum (ATCC 29335).	Tenckhoff B, Kolmel HW, Wolf V, Lange R	Zentralbl Bakteriol	10.1016/s0934-8840(11)80605-5	1994	*

References

#16394

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22553

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83501

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID39477.1 )

* These data were automatically processed and therefore are not curated

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Spiroplasma mirum ATCC 29335 ATCC 29335

Spiroplasma mirum ATCC 29335 ATCC 29335

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