Strain identifier

BacDive ID: 14289

Type strain: Yes

Species: Zymomonas mobilis

Strain Designation: pZM01 to pZM06

Variant: Isotype of BacDive ID 132836

Strain history: CIP <- 1986, NCIB <- 1958, S.R. Elsden <- 1958, ATCC <- NRRL: strain B-806, Pseudomonas lindneri

NCBI tax ID(s): 542 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 194

BacDive-ID: 14289

DSM-Number: 424

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, rod-shaped, alcohol production

description: Zymomonas mobilis pZM01 to pZM06 is an anaerobe, mesophilic, Gram-negative bacterium that produces alcohol and was isolated from Agave sap.

NCBI tax id

  • NCBI tax id: 542
  • Matching level: species

strain history

@refhistory
194<- IMG <- E.A. Dawes <- NCIB <- S.R. Elsden <- ATCC
67770IAM 12663 <-- NRRL ("Pseudomonas lindneri").
120991CIP <- 1986, NCIB <- 1958, S.R. Elsden <- 1958, ATCC <- NRRL: strain B-806, Pseudomonas lindneri

doi: 10.13145/bacdive14289.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Sphingomonadales
  • family: Zymomonadaceae
  • genus: Zymomonas
  • species: Zymomonas mobilis
  • full scientific name: Zymomonas mobilis corrig. (Lindner 1928) Kluyver and van Niel 1936 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Zymomonas mobile
    20215Thermobacterium mobile

@ref: 194

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Sphingomonadales

family: Zymomonadaceae

genus: Zymomonas

species: Zymomonas mobilis

full scientific name: Zymomonas mobilis (Lindner 1928) Kluyver and van Niel 1936

strain designation: pZM01 to pZM06

variant: Isotype of BacDive ID 132836

type strain: yes

Morphology

cell morphology

  • @ref: 120991
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
194ZYMOMONAS MEDIUM (DSMZ Medium 10)yeshttps://mediadive.dsmz.de/medium/10Name: ZYMOMONAS MEDIUM (DSMZ Medium 10) Composition: Glucose 20.0 g/l Agar 15.0 g/l Yeast extract 10.0 g/l Bacto peptone 10.0 g/l Distilled water
40432MEDIUM 77 - for Zymomonas mobilisyesDistilled water make up to (1000.000 ml);Glucose (20.000 g);Yeast extract (5.000 g)
120991CIP Medium 77yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=77

culture temp

@refgrowthtypetemperaturerange
194positivegrowth30mesophilic
40432positivegrowth30mesophilic
67770positivegrowth30mesophilic
120991positivegrowth25-37mesophilic
120991nogrowth10psychrophilic
120991nogrowth15psychrophilic
120991nogrowth41thermophilic
120991nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
194anaerobe
120991anaerobe

compound production

  • @ref: 194
  • compound: ethanol

halophily

@refsaltgrowthtested relationconcentration
120991NaClnogrowth0 %
120991NaClnogrowth2 %
120991NaClnogrowth4 %
120991NaClnogrowth6 %
120991NaClnogrowth8 %
120991NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1209914853esculin-hydrolysis
12099117632nitrate-reduction
12099116301nitrite-reduction
12099117632nitrate+respiration

antibiotic resistance

  • @ref: 120991
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 120991
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12099115688acetoin-
12099117234glucose-

enzymes

@refvalueactivityec
120991oxidase-
120991beta-galactosidase-3.2.1.23
120991alcohol dehydrogenase-1.1.1.1
120991gelatinase-
120991amylase-
120991DNase-
120991caseinase-3.4.21.50
120991catalase+1.11.1.6
120991tween esterase-
120991gamma-glutamyltransferase-2.3.2.2
120991lecithinase-
120991lipase-
120991lysine decarboxylase-4.1.1.18
120991ornithine decarboxylase-4.1.1.17
120991phenylalanine ammonia-lyase-4.3.1.24
120991protease-
120991tryptophan deaminase-
120991urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120991-+++-++++-++--------

Isolation, sampling and environmental information

isolation

  • @ref: 67770
  • sample type: Agave sap
  • country: Mexico
  • origin.country: MEX
  • continent: North America

taxonmaps

  • @ref: 69479
  • File name: preview.99_337.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_92;96_237;97_259;98_288;99_337&stattab=map
  • Last taxonomy: Zymomonas mobilis subclade
  • 16S sequence: AY670647
  • Sequence Identity:
  • Total samples: 2581
  • soil counts: 1083
  • aquatic counts: 916
  • animal counts: 421
  • plant counts: 161

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
1941Risk group (German classification)
1209911Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Zymomonas mobilis strain ATCC10988 16S ribosomal RNA gene, partial sequenceAF2810311452ena555217
20218Zymomonas mobilis strain ATCC 10988 16S-23S internal transcribed spacer, complete sequenceAF329200231ena555217
20218Zymomonas mobilis subsp. mobilis strain LMG 404 16S ribosomal RNA gene, partial sequenceAY6706471343ena555217
20218Zymomonas mobilis subsp. mobilis strain LMG 404 16S-23S ribosomal RNA intergenic spacer, partial sequence; tRNA-Ile and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequenceAY940084670ena555217

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Zymomonas mobilis subsp. mobilis ATCC 10988GCA_000175255completencbi555217
66792Zymomonas mobilis subsp. mobilis ATCC 10988555217.8wgspatric555217
66792Zymomonas mobilis subsp. mobilis ATCC 10988555217.15plasmidpatric555217
66792Zymomonas mobilis subsp. mobilis ATCC 10988555217.16plasmidpatric555217
66792Zymomonas mobilis subsp. mobilis ATCC 10988555217.17plasmidpatric555217
66792Zymomonas mobilis subsp. mobilis ATCC 10988555217.24plasmidpatric555217
66792Zymomonas mobilis subsp. mobilis ATCC 10988555217.22plasmidpatric555217
66792Zymomonas mobilis subsp. mobilis ATCC 10988555217.20plasmidpatric555217
66792Zymomonas mobilis subsp. mobilis ATCC 10988555217.19plasmidpatric555217
66792Zymomonas mobilis subsp. mobilis ATCC 10988555217.23plasmidpatric555217
66792Zymomonas mobilis subsp. mobilis ATCC 10988555217.18plasmidpatric555217
66792Zymomonas mobilis subsp. mobilis ATCC 10988555217.21plasmidpatric555217
66792Zymomonas mobilis mobilis T.H.Delft 1, ATCC 10988645058785completeimg555217

GC content

  • @ref: 194
  • GC-content: 49.1

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes78.482no
flagellatedno75.496no
gram-positiveno96.888no
anaerobicno94.195no
aerobicno90.621yes
halophileno80.396no
spore-formingno96.511no
glucose-utilyes78.891no
thermophileno86.303yes
glucose-fermentno63.852no

External links

@ref: 194

culture collection no.: DSM 424, ATCC 10988, NCIB 8938, NRRL B-806, IMG 1655, JCM 20722, CIP 102538, IAM 12663, ICPB 2463, NCIMB 8938, LMG 404

literature

Pubmed-IDtitleauthorsjournalDOIyearmeshtopictopic2
2349282Analysis and stability of Zymomonas mobilis ATCC 10988 plasmid pZMO3.Scordaki A, Drainas CPlasmid10.1016/0147-619x(90)90044-d1990Chromosomes, Bacterial, Conjugation, Genetic, DNA, Bacterial/genetics, Gram-Negative Bacteria/*genetics, *Plasmids, Restriction Mapping, Sequence Homology, Nucleic Acid
3309139Expression and stability of a recombinant plasmid in Zymomonas mobilis and Escherichia coli.Afendra AS, Drainas CJ Gen Microbiol10.1099/00221287-133-1-1271987DNA, Bacterial, DNA, Recombinant, Electrophoresis, Agar Gel, Escherichia coli/*genetics, *Gene Expression Regulation, *Genetic Vectors, Gram-Negative Anaerobic Bacteria/*genetics, *Plasmids, *Transformation, BacterialEnzymology
6856691Comparison of plasmids in strains of Zymomonas mobilis.Stokes HW, Dally EL, Yablonsky MD, Eveleigh DEPlasmid10.1016/0147-619x(83)90016-11983Anti-Bacterial Agents/pharmacology, Bacteria/drug effects/*genetics, DNA, Bacterial/genetics, Drug Resistance, Microbial, Molecular Weight, Nucleic Acid Hybridization, *Plasmids, Species SpecificityPhylogenyPathogenicity
8086457Nucleotide and derived amino acid sequences of an extracellular sucrase gene (invB) of Zymomonas mobilis ZM1 (ATCC10988).Song KB, Lee SK, Joo HK, Rhee SKBiochim Biophys Acta10.1016/0167-4781(94)90262-31994Amino Acid Sequence, Bacterial Proteins/*genetics/metabolism, Base Sequence, Genes, Bacterial/*genetics, Molecular Sequence Data, Sequence Analysis, Sequence Homology, Amino Acid, Sucrase/*genetics/metabolism, Zymomonas/enzymology/*geneticsEnzymologyMetabolism
8318541Nucleotide sequence of levansucrase gene (levU) of Zymomonas mobilis ZM1 (ATCC10988).Song KB, Joo HK, Rhee SKBiochim Biophys Acta10.1016/0167-4781(93)90130-61993Amino Acid Sequence, Base Sequence, Cloning, Molecular, *Genes, *Genes, Bacterial, Hexosyltransferases/*genetics, Molecular Sequence Data, Zymomonas/enzymology/*geneticsEnzymologyGenetics
8441765An ultraviolet-sensitive mutant of Zymomonas mobilis affecting the stability of its natural plasmid pZMO2.Vartholomatos G, Typas MA, Drainas CPlasmid10.1006/plas.1993.10021993Conjugation, Genetic, DNA Repair/genetics/radiation effects, Drug Resistance, Microbial/genetics, Genes, Bacterial, Genetic Complementation Test, Methyl Methanesulfonate, Mutation, Plasmids/*radiation effects, Restriction Mapping, Ultraviolet Rays, Zymomonas/drug effects/*genetics/radiation effectsPathogenicity
9887309Characterization of the mobilization region of the Zymomonas mobilis ATCC10988 plasmid pZMO3.Afendra AS, Vartholomatos G, Arvanitis N, Drainas CPlasmid10.1006/plas.1998.13741999Bacterial Proteins/genetics, Base Sequence, Conjugation, Genetic, Gene Deletion, Gene Transfer Techniques, Molecular Sequence Data, Open Reading Frames, Plasmids/*genetics, Recombinant Proteins/genetics, Zymomonas/*geneticsGenetics
10964623Characterization and replication properties of the Zymomonas mobilis ATCC 10988 plasmids pZMO1 and pZMO2.Arvanitis N, Pappas KM, Kolios G, Afendra AS, Typas MA, Drainas CPlasmid10.1006/plas.2000.14802000Amino Acid Sequence, Bacterial Proteins/chemistry/genetics, Base Sequence, Conjugation, Genetic, DNA Helicases/chemistry/*genetics, *DNA Replication, DNA, Single-Stranded/chemistry/genetics, *DNA-Binding Proteins, Molecular Sequence Data, Open Reading Frames, Plasmids/*genetics, Sequence Alignment, Sequence Homology, Amino Acid, Trans-Activators/chemistry/*genetics, Zymomonas/*geneticsGenetics
11381333A simple and efficient method for the purification of membrane-bound levansucrase from Zymomonas mobilis.Vigants A, Hicke HG, Marx SPCurr Microbiol10.1007/s0028400102392001Cell Membrane/enzymology, Chromatography/methods, Chromatography, Ion Exchange/methods, Electrophoresis, Polyacrylamide Gel/methods, Hexosyltransferases/*isolation & purification, Kinetics, Zymomonas/*enzymology/physiologyEnzymologyPhylogeny
15592456The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4.Seo JS, Chong H, Park HS, Yoon KO, Jung C, Kim JJ, Hong JH, Kim H, Kim JH, Kil JI, Park CJ, Oh HM, Lee JS, Jin SJ, Um HW, Lee HJ, Oh SJ, Kim JY, Kang HL, Lee SY, Lee KJ, Kang HSNat Biotechnol10.1038/nbt10452004Biological Transport, DNA/chemistry, Ethanol/chemistry/metabolism, Genome, *Genome, Bacterial, Ketoglutarate Dehydrogenase Complex/metabolism, Malate Dehydrogenase/metabolism, Models, Biological, Molecular Sequence Data, Multigene Family, Oligonucleotide Array Sequence Analysis, Open Reading Frames, Oxidative Stress, Plasmids/metabolism, RNA/chemistry, Sequence Analysis, DNA, Transcription, Genetic, Tricarboxylic Acids/metabolism, Zymomonas/*geneticsGeneticsMetabolism
16232532Investigation of the utility of pineapple juice and pineapple waste material as low-cost substrate for ethanol fermentation by Zymomonas mobilis.Tanaka K, Hilary ZD, Ishizaki AJ Biosci Bioeng10.1016/s1389-1723(99)80128-51999
18546408Ethanol production by immobilized Saccharomyces cerevisiae, Saccharomyces uvarum, and Zymomonas mobilis.McGhee JE, Julian GS, Detroy RW, Bothast RJBiotechnol Bioeng10.1002/bit.2602405121982Biotechnology
18592556Immobilized cell biocatalyst activation and pseudo-steady-state behavior: model and experiment.Monbouquette HG, Sayles GD, Ollis DFBiotechnol Bioeng10.1002/bit.2603506081990
18597261A structured kinetic model for Zymomonas mobilis ATCC10988.Veeramallu U, Agrawal PBiotechnol Bioeng10.1002/bit.2603607071990
21725006Genome sequence of the ethanol-producing Zymomonas mobilis subsp. mobilis lectotype strain ATCC 10988.Pappas KM, Kouvelis VN, Saunders E, Brettin TS, Bruce D, Detter C, Balakireva M, Han CS, Savvakis G, Kyrpides NC, Typas MAJ Bacteriol10.1128/JB.05395-112011Agave/microbiology, DNA, Bacterial/*chemistry/*genetics, Ethanol/metabolism, Fermentation, Food Microbiology, *Genome, Bacterial, Mexico, Molecular Sequence Data, Plasmids, *Sequence Analysis, DNA, Sucrose/metabolism, Zymomonas/*genetics/isolation & purification/metabolism/physiologyGeneticsMetabolism
24629064pZMO7-Derived shuttle vectors for heterologous protein expression and proteomic applications in the ethanol-producing bacterium Zymomonas mobilis.So LY, Chen WY, Lacap-Bugler DC, Seemann M, Watt RMBMC Microbiol10.1186/1471-2180-14-682014Escherichia coli/genetics, Ethanol/*metabolism, *Gene Expression, *Genetic Vectors, Genetics, Microbial/*methods, Genomic Instability, Protein Binding, Protein Interaction Mapping, Proteomics/*methods, Zymomonas/*genetics/*metabolismMetabolism
26883123Reconstruction of a charge balanced genome-scale metabolic model to study the energy-uncoupled growth of Zymomonas mobilis ZM1.Motamedian E, Saeidi M, Shojaosadati SAMol Biosyst10.1039/c5mb00588d2016Adenosine Triphosphatases/metabolism, Algorithms, Computer Simulation, Ethanol/metabolism, Fermentation, *Genome-Wide Association Study, Glucose/metabolism, *Metabolome, *Metabolomics/methods, *Models, Biological, Oxygen Consumption, Proton-Translocating ATPases/metabolism, Reproducibility of Results, Zymomonas/*genetics/*metabolismMetabolismGenetics
27541979Unconventional bacterial association for dough leavening.Musatti A, Mapelli C, Foschino R, Picozzi C, Rollini MInt J Food Microbiol10.1016/j.ijfoodmicro.2016.08.0112016Bread/analysis/microbiology, Ethanol/metabolism, Fermentation, Food Microbiology, Fructose/metabolism, Glucose/metabolism, Hydrolysis, Lactobacillus/growth & development/*metabolism, Microbiota, Sucrose/metabolism, Triticum/microbiology, Zymomonas/growth & development/*metabolismMetabolismBiotechnology
29694430Genome comparison of different Zymomonas mobilis strains provides insights on conservation of the evolution.Chen C, Wu L, Cao Q, Shao H, Li X, Zhang Y, Wang H, Tan XPLoS One10.1371/journal.pone.01959942018Ethanol/metabolism, Evolution, Molecular, Genome Size, *Genome, Bacterial, Hydrogen-Ion Concentration, Phylogeny, Sequence Analysis, DNA/*methods, Zymomonas/*classification/genetics/metabolismGeneticsPhylogeny
32466402Yeast-Free Doughs by Zymomonas mobilis: Evaluation of Technological and Fermentation Performances by Using a Metabolomic Approach.Nissen L, Rollini M, Picozzi C, Musatti A, Foschino R, Gianotti AMicroorganisms10.3390/microorganisms80607922020

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
194Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 424)https://www.dsmz.de/collection/catalogue/details/culture/DSM-424
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40432Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14236
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
120991Curators of the CIPCollection of Institut Pasteur (CIP 102538)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102538