Strain identifier

BacDive ID: 1422

Type strain: Yes

Species: Geobacillus thermocatenulatus

Strain Designation: R-35648, 178

Strain history: <- L.G. Loginova, 178

NCBI tax ID(s): 33938 (species)

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General

@ref: 375

BacDive-ID: 1422

DSM-Number: 730

keywords: 16S sequence, Bacteria, facultative anaerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Geobacillus thermocatenulatus R-35648 is a facultative anaerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from hot-gas well, coatings inside tube.

NCBI tax id

  • NCBI tax id: 33938
  • Matching level: species

strain history

  • @ref: 375
  • history: <- L.G. Loginova, 178

doi: 10.13145/bacdive1422.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Geobacillus
  • species: Geobacillus thermocatenulatus
  • full scientific name: Geobacillus thermocatenulatus (Golovacheva et al. 1991) Nazina et al. 2001

  • synonyms

    @refsynonym
    20215Geobacillus gargensis
    20215Bacillus thermocatenulatus

@ref: 375

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Geobacillus

species: Geobacillus thermocatenulatus

full scientific name: Geobacillus thermocatenulatus (Golovacheva et al. 1991) Nazina et al. 2001 emend. Dinsdale et al. 2011

strain designation: R-35648, 178

type strain: yes

Morphology

cell morphology

  • @ref: 23043
  • gram stain: positive
  • cell length: 6.0-12.0 µm
  • cell width: 0.9-1.5 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: peritrichous

colony morphology

  • @ref: 23043
  • colony color: cream or yellowish
  • colony shape: circular
  • medium used: trypticase soy agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
23043trypticase soy agaryes
375NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
23043positivegrowth37.0mesophilic
23043positivegrowth80.0hyperthermophilic
23043positiveoptimum60.0thermophilic
375positivegrowth60thermophilic

culture pH

@refabilitytypepHPH range
23043positivegrowth5.0
23043positivegrowth9.0alkaliphile
23043positiveoptimum7.0

Physiology and metabolism

oxygen tolerance

  • @ref: 23043
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 23043
  • spore description: ellipsoidal or cylindrical,terminal or subterminal or paracentral,do not swell the sporangia
  • type of spore: endospore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
23043NaClpositivegrowth5.0 %
23043NaClnogrowth7.0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
23043168082-dehydro-D-gluconate-builds acid from
23043174265-dehydro-D-gluconate-builds acid from
2304315963ribitol-builds acid from
2304317108D-arabinose-builds acid from
2304318333D-arabitol-builds acid from
2304328847D-fucose-builds acid from
2304362318D-lyxose-builds acid from
2304316443D-tagatose-builds acid from
2304365327D-xylose-builds acid from
2304316813galactitol-builds acid from
2304317113erythritol-builds acid from
2304328066gentiobiose-builds acid from
2304324265gluconate-builds acid from
2304328087glycogen-builds acid from
2304315443inulin-builds acid from
2304330849L-arabinose-builds acid from
2304318403L-arabitol-builds acid from
2304318287L-fucose-builds acid from
2304317266L-sorbose-builds acid from
2304365328L-xylose-builds acid from
2304317716lactose-builds acid from
2304317268myo-inositol-builds acid from
2304326546rhamnose-builds acid from
2304330911sorbitol-builds acid from
2304332528turanose-builds acid from
2304317151xylitol-builds acid from
2304316947citrate-carbon source
2304327897tryptophan-energy source
23043casein-hydrolysis
23043O-nitrophenyl-beta-D-galactopyranosid-hydrolysis
2304328017starch-hydrolysis
2304317632nitrate-reduction
2304327613amygdalin+builds acid from
2304318305arbutin+builds acid from
2304317057cellobiose+builds acid from
2304315824D-fructose+builds acid from
2304317634D-glucose+builds acid from
2304316024D-mannose+builds acid from
2304328260galactose+builds acid from
2304317754glycerol+builds acid from
2304317306maltose+builds acid from
2304329864mannitol+builds acid from
230436731melezitose+builds acid from
2304328053melibiose+builds acid from
2304337657methyl D-glucoside+builds acid from
23043506227N-acetylglucosamine+builds acid from
2304316634raffinose+builds acid from
2304333942ribose+builds acid from
2304317814salicin+builds acid from
2304328017starch+builds acid from
2304317992sucrose+builds acid from
2304327082trehalose+builds acid from
2304327613amygdalin+builds gas from
2304318305arbutin+builds gas from
2304317057cellobiose+builds gas from
2304315824D-fructose+builds gas from
2304317634D-glucose+builds gas from
2304316024D-mannose+builds gas from
2304328260galactose+builds gas from
2304317754glycerol+builds gas from
2304317306maltose+builds gas from
2304329864mannitol+builds gas from
230436731melezitose+builds gas from
2304328053melibiose+builds gas from
2304337657methyl D-glucoside+builds gas from
23043506227N-acetylglucosamine+builds gas from
2304316634raffinose+builds gas from
2304333942ribose+builds gas from
2304317814salicin+builds gas from
2304328017starch+builds gas from
2304317992sucrose+builds gas from
2304327082trehalose+builds gas from
230434853esculin+hydrolysis
230435291gelatin+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
2304335581indoleno
2304316136hydrogen sulfideno
2304315688acetoinyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testcitrate test
2304315688acetoin+
2304335581indole-
2304316947citrate-

enzymes

@refvalueactivityec
23043arginine dihydrolase-3.5.3.6
23043beta-galactosidase-3.2.1.23
23043catalase+1.11.1.6
23043cytochrome oxidase+1.9.3.1
23043lysine decarboxylase-4.1.1.18
23043ornithine decarboxylase-4.1.1.17
23043tryptophan deaminase-4.1.99.1
23043urease-3.5.1.5

Isolation, sampling and environmental information

isolation

  • @ref: 375
  • sample type: hot-gas well, coatings inside tube
  • geographic location: southern Ural
  • country: Russia
  • origin.country: RUS

isolation source categories

Cat1Cat2
#Engineered#Built environment
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_882.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_79;96_191;97_207;98_231;99_882&stattab=map
  • Last taxonomy: Geobacillus
  • 16S sequence: Z26926
  • Sequence Identity:
  • Total samples: 794
  • soil counts: 50
  • aquatic counts: 158
  • animal counts: 556
  • plant counts: 30

Safety information

risk assessment

  • @ref: 375
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Geobacillus sp. NBRC 15316 gene for 16S rRNA, partial sequenceAB6808351483ena1113648
20218Geobacillus thermocatenulatus strain DSM 730 16S-23S ribosomal RNA intergenic spacer and tRNA-Ile gene, partial sequence; and tRNA-Ala gene, complete sequenceEU157952397ena33938
20218Geobacillus thermocatenulatus isolate Gt2 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceEU723637622ena33938
20218Geobacillus thermocatenulatus isolate Gt4 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceEU723638507ena33938
20218Geobacillus thermocatenulatus isolate Gt43 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceEU723639376ena33938
20218Geobacillus thermocatenulatus isolate Gt45 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceEU7236401021ena33938
20218B.thermocatenulatus gene for 16S ribosomal RNAZ269261523ena33938
375Geobacillus thermocatenulatus strain BGSC 93A1 16S ribosomal RNA gene, complete sequenceAY6089351561ena33938
375Geobacillus thermocatenulatus partial 16S rRNA gene, strain R-35648FN5389891560ena33938

GC content

@refGC-contentmethod
37551.17high performance liquid chromatography (HPLC)
37569.0

External links

@ref: 375

culture collection no.: DSM 730, BGSC 93, IFO 15316, LMG 19007, NBRC 15316, VKM B-1259

straininfo link

  • @ref: 71072
  • straininfo: 12966

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology8068728Screening, purification and properties of a thermophilic lipase from Bacillus thermocatenulatus.Schmidt-Dannert C, Sztajer H, Stocklein W, Menge U, Schmid RDBiochim Biophys Acta10.1016/0005-2760(94)90008-61994Amino Acid Sequence, Bacillus/*enzymology, Chemical Precipitation, Chromatography, Ion Exchange, Detergents/pharmacology, Electrophoresis, Polyacrylamide Gel, Enzyme Stability, Hexanes, *Hot Temperature, Hydrogen-Ion Concentration, Lipase/chemistry/isolation & purification/*metabolism, Methanol, Molecular Sequence Data, Molecular Weight, Substrate SpecificityStress
29483968High Quality Draft Genomes of the Type Strains Geobacillus thermocatenulatus DSM 730(T), G. uzenensis DSM 23175(T) And Parageobacillus galactosidasius DSM 18751(T).Ramaloko WT, Koen N, Polliack S, Aliyu H, Lebre PH, Mohr T, Oswald F, Zwick M, Zeigler DR, Neumann A, Syldatk C, Cowan DA, De Maayer PJ Genomics10.7150/jgen.229862018

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
375Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 730)https://www.dsmz.de/collection/catalogue/details/culture/DSM-730
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23043Anna E. Dinsdale,Gillian Halket,An Coorevits,Anita Van Landschoot,Hans-Jürgen Busse,Paul De Vos,Niall A. Logan10.1099/ijs.0.025445-0Emended descriptions of Geobacillus thermoleovorans and Geobacillus thermocatenulatusIJSEM 61: 1802-1810 201120817844
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71072Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID12966.1StrainInfo: A central database for resolving microbial strain identifiers