Name: Geobacillus thermocatenulatus R-35648
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1422

Type strain:

Species: Geobacillus thermocatenulatus

Strain Designation: R-35648, 178

Culture col. no.: DSM 730, BGSC 93, IFO 15316, LMG 19007, NBRC 15316, VKM B-1259

Strain history: <- L.G. Loginova, 178

NCBI tax ID(s): 33938 (species)

SI-ID 12966

LMG 19007, DSM 730, IFO 15316, VKM B-1259, CCM 2809, NBRC 15316, KCTC 3921, KACC 11364, BCRC 17466, CCRC 17466

Other strains from Geobacillus thermocatenulatus

G. thermocatenulatus Ga, DSM 15378, VKM B-2300

Section

Geobacillus thermocatenulatus R-35648 is a facultative anaerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from hot-gas well, coatings inside tube.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
facultative anaerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Geobacillus*
	Species	**Geobacillus thermocatenulatus**
	Full Scientific Name (LPSN)	Geobacillus thermocatenulatus (Golovacheva et al. 1991) Nazina et al. 2001

	Synonym	Geobacillus gargensis
	Synonym	Bacillus thermocatenulatus

Information on morphological and physiological properties Morphology

[Ref.: #23043]	Gram stain	positive

[Ref.: #23043]	Cell length	6.0-12.0 µm

[Ref.: #23043]	Cell width	0.9-1.5 µm

[Ref.: #23043]	Cell shape	rod-shaped

[Ref.: #23043]	Motility	yes

[Ref.: #23043]	Flagellum arrangement	peritrichous

[Ref.: #23043]	Colony color	cream or yellowish
[Ref.: #23043]	Colony shape	circular
[Ref.: #23043]	Cultivation medium used	trypticase soy agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #23043]

Culture medium

trypticase soy agar

[Ref.: #23043]

Culture medium growth

[Ref.: #375]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #375]

Culture medium growth

[Ref.: #375]

Culture medium link

Medium recipe at MediaDive

[Ref.: #375]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #375]	growth	60 °C
[Ref.: #23043]	growth	37.0 °C
[Ref.: #23043]	growth	80.0 °C
[Ref.: #23043]	optimum	60.0 °C

[Ref.: #375]	Temperature range	thermophilic
[Ref.: #23043]	Temperature range	mesophilic
[Ref.: #23043]	Temperature range	hyperthermophilic
[Ref.: #23043]	Temperature range	thermophilic

		pH
[Ref.: #23043]	optimum	7.0
[Ref.: #23043]	growth	5.0
[Ref.: #23043]	growth	9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23043]

Oxygen tolerance

facultative anaerobe

[Ref.: #23043]	Spore description	ellipsoidal or cylindrical,terminal or subterminal or paracentral,do not swell the sporangia
[Ref.: #23043]	Type of spore	endospore
[Ref.: #23043]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23043]		NaCl		growth	5.0	%
[Ref.: #23043]		NaCl	no	growth	7.0	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23043]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23043]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23043]	amygdalin ChEBI	+	builds acid from
[Ref.: #23043]	amygdalin ChEBI	+	builds gas from
[Ref.: #23043]	arbutin ChEBI	+	builds acid from
[Ref.: #23043]	arbutin ChEBI	+	builds gas from
[Ref.: #23043]	casein	-	hydrolysis
[Ref.: #23043]	cellobiose ChEBI	+	builds acid from
[Ref.: #23043]	cellobiose ChEBI	+	builds gas from
[Ref.: #23043]	citrate ChEBI	-	carbon source
[Ref.: #23043]	D-arabinose ChEBI	-	builds acid from
[Ref.: #23043]	D-arabitol ChEBI	-	builds acid from
[Ref.: #23043]	D-fructose ChEBI	+	builds acid from
[Ref.: #23043]	D-fructose ChEBI	+	builds gas from
[Ref.: #23043]	D-fucose ChEBI	-	builds acid from
[Ref.: #23043]	D-glucose ChEBI	+	builds acid from
[Ref.: #23043]	D-glucose ChEBI	+	builds gas from
[Ref.: #23043]	D-lyxose ChEBI	-	builds acid from
[Ref.: #23043]	D-mannose ChEBI	+	builds acid from
[Ref.: #23043]	D-mannose ChEBI	+	builds gas from
[Ref.: #23043]	D-tagatose ChEBI	-	builds acid from
[Ref.: #23043]	D-xylose ChEBI	-	builds acid from
[Ref.: #23043]	erythritol ChEBI	-	builds acid from
[Ref.: #23043]	esculin ChEBI	+	hydrolysis
[Ref.: #23043]	galactitol ChEBI	-	builds acid from
[Ref.: #23043]	galactose ChEBI	+	builds acid from
[Ref.: #23043]	galactose ChEBI	+	builds gas from
[Ref.: #23043]	gelatin ChEBI	+	hydrolysis
[Ref.: #23043]	gentiobiose ChEBI	-	builds acid from
[Ref.: #23043]	gluconate ChEBI	-	builds acid from
[Ref.: #23043]	glycerol ChEBI	+	builds acid from
[Ref.: #23043]	glycerol ChEBI	+	builds gas from
[Ref.: #23043]	glycogen ChEBI	-	builds acid from
[Ref.: #23043]	inulin ChEBI	-	builds acid from
[Ref.: #23043]	L-arabinose ChEBI	-	builds acid from
[Ref.: #23043]	L-arabitol ChEBI	-	builds acid from
[Ref.: #23043]	L-fucose ChEBI	-	builds acid from
[Ref.: #23043]	L-sorbose ChEBI	-	builds acid from
[Ref.: #23043]	L-xylose ChEBI	-	builds acid from
[Ref.: #23043]	lactose ChEBI	-	builds acid from
[Ref.: #23043]	maltose ChEBI	+	builds acid from
[Ref.: #23043]	maltose ChEBI	+	builds gas from
[Ref.: #23043]	mannitol ChEBI	+	builds acid from
[Ref.: #23043]	mannitol ChEBI	+	builds gas from
[Ref.: #23043]	melezitose ChEBI	+	builds acid from
[Ref.: #23043]	melezitose ChEBI	+	builds gas from
[Ref.: #23043]	melibiose ChEBI	+	builds acid from
[Ref.: #23043]	melibiose ChEBI	+	builds gas from
[Ref.: #23043]	methyl D-glucoside ChEBI	+	builds acid from
[Ref.: #23043]	methyl D-glucoside ChEBI	+	builds gas from
[Ref.: #23043]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23043]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #23043]	N-acetylglucosamine ChEBI	+	builds gas from
[Ref.: #23043]	nitrate ChEBI	-	reduction
[Ref.: #23043]	O-nitrophenyl-beta-D-galactopyranosid	-	hydrolysis
[Ref.: #23043]	raffinose ChEBI	+	builds acid from
[Ref.: #23043]	raffinose ChEBI	+	builds gas from
[Ref.: #23043]	rhamnose ChEBI	-	builds acid from
[Ref.: #23043]	ribitol ChEBI	-	builds acid from
[Ref.: #23043]	ribose ChEBI	+	builds acid from
[Ref.: #23043]	ribose ChEBI	+	builds gas from
[Ref.: #23043]	salicin ChEBI	+	builds acid from
[Ref.: #23043]	salicin ChEBI	+	builds gas from
[Ref.: #23043]	sorbitol ChEBI	-	builds acid from
[Ref.: #23043]	starch ChEBI	-	hydrolysis
[Ref.: #23043]	starch ChEBI	+	builds acid from
[Ref.: #23043]	starch ChEBI	+	builds gas from
[Ref.: #23043]	sucrose ChEBI	+	builds acid from
[Ref.: #23043]	sucrose ChEBI	+	builds gas from
[Ref.: #23043]	trehalose ChEBI	+	builds acid from
[Ref.: #23043]	trehalose ChEBI	+	builds gas from
[Ref.: #23043]	tryptophan ChEBI	-	energy source
[Ref.: #23043]	turanose ChEBI	-	builds acid from
[Ref.: #23043]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #23043]	acetoin ChEBI	yes
[Ref.: #23043]	hydrogen sulfide ChEBI	no
[Ref.: #23043]	indole ChEBI	no

	Metabolite	Voges-Proskauer-test	Indole test	Citrate test
[Ref.: #23043]	acetoin ChEBI	+
[Ref.: #23043]	citrate ChEBI			-
[Ref.: #23043]	indole ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #23043]	arginine dihydrolase	-	3.5.3.6
[Ref.: #23043]	beta-galactosidase	-	3.2.1.23
[Ref.: #23043]	catalase	+	1.11.1.6
[Ref.: #23043]	cytochrome oxidase	+	1.9.3.1
[Ref.: #23043]	lysine decarboxylase	-	4.1.1.18
[Ref.: #23043]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #23043]	tryptophan deaminase	-	4.1.99.1
[Ref.: #23043]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #375]	Sample type/isolated from	hot-gas well, coatings inside tube
[Ref.: #375]	Geographic location (country and/or sea, region)	southern Ural
[Ref.: #375]	Country	Russia
[Ref.: #375]	Country ISO 3 Code	RUS
* marker position based on {}

Isolation sources categories

#Engineered	#Built environment	-
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence Z26926 (>99% sequence identity) for Geobacillus from Microbeatlas

Aquatic Samples	158
Soil Samples	50
Animal Samples	556
Plant Samples	30
Total Samples	794

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #375]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #375]	Geobacillus thermocatenulatus partial 16S rRNA gene, strain R-35648	FN538989	1560	ENA	33938 tax ID
[Ref.: #375]	Geobacillus thermocatenulatus strain BGSC 93A1 16S ribosomal RNA gene, complete sequence	AY608935	1561	ENA	33938 tax ID
[Ref.: #20218]	Geobacillus thermocatenulatus strain DSM 730 16S-23S ribosomal RNA intergenic spacer and tRNA-Ile gene, partial sequence; and tRNA-Ala gene, complete sequence	EU157952	397	ENA	33938 tax ID	*
[Ref.: #20218]	Geobacillus sp. NBRC 15316 gene for 16S rRNA, partial sequence	AB680835	1483	ENA	1113648 tax ID	*
[Ref.: #20218]	Geobacillus thermocatenulatus isolate Gt2 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723637	622	ENA	33938 tax ID	*
[Ref.: #20218]	B.thermocatenulatus gene for 16S ribosomal RNA	Z26926	1523	ENA	33938 tax ID	*
[Ref.: #20218]	Geobacillus thermocatenulatus isolate Gt4 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723638	507	ENA	33938 tax ID	*
[Ref.: #20218]	Geobacillus thermocatenulatus isolate Gt43 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723639	376	ENA	33938 tax ID	*
[Ref.: #20218]	Geobacillus thermocatenulatus isolate Gt45 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723640	1021	ENA	33938 tax ID	*

[Ref.: #375]	GC-content	51.17 mol%	high performance liquid chromatography (HPLC)
[Ref.: #375]	GC-content	69.0 mol%

Availability in culture collections External links

[Ref.: #375]

Culture collection no.

DSM 730, BGSC 93, IFO 15316, LMG 19007, NBRC 15316, VKM B-1259

[Ref.: #71072]

SI-ID 12966

Literature:

	Title	Authors	Journal	DOI	Year
Enzymology	Screening, purification and properties of a thermophilic lipase from Bacillus thermocatenulatus.	Schmidt-Dannert C, Sztajer H, Stocklein W, Menge U, Schmid RD	Biochim Biophys Acta	10.1016/0005-2760(94)90008-6	1994	*
	High Quality Draft Genomes of the Type Strains Geobacillus thermocatenulatus DSM 730(T), G. uzenensis DSM 23175(T) And Parageobacillus galactosidasius DSM 18751(T).	Ramaloko WT, Koen N, Polliack S, Aliyu H, Lebre PH, Mohr T, Oswald F, Zwick M, Zeigler DR, Neumann A, Syldatk C, Cowan DA, De Maayer P	J Genomics	10.7150/jgen.22986	2018	*

References

#375	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 730

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23043

Anna E. Dinsdale,Gillian Halket,An Coorevits,Anita Van Landschoot,Hans-Jürgen Busse,Paul De Vos,Niall A. Logan: Emended descriptions of Geobacillus thermoleovorans and Geobacillus thermocatenulatus. IJSEM 61: 1802 - 1810 2011 ( DOI 10.1099/ijs.0.025445-0 , PubMed 20817844 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#71072

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID12966.1 )

* These data were automatically processed and therefore are not curated

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