Strain identifier
BacDive ID: 141134
Type strain:
Species: Flavobacterium paronense
Strain Designation: KNUS1
NCBI tax ID(s): 1392775 (species)
General
@ref: 44049
BacDive-ID: 141134
keywords: 16S sequence, Bacteria, obligate aerobe, Gram-negative, motile, rod-shaped, colony-forming
description: Flavobacterium paronense KNUS1 is an obligate aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from freshwater near a floating vegetated island from a lake.
NCBI tax id
- NCBI tax id: 1392775
- Matching level: species
doi: 10.13145/bacdive141134.20240510.9
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacteroidota
- domain: Bacteria
- phylum: Bacteroidota
- class: Flavobacteriia
- order: Flavobacteriales
- family: Flavobacteriaceae
- genus: Flavobacterium
- species: Flavobacterium paronense
- full scientific name: Flavobacterium paronense Joung et al. 2016
@ref: 44049
domain: Bacteria
phylum: Bacteroidetes
class: Flavobacteriia
order: Flavobacteriales
family: Flavobacteriaceae
genus: Flavobacterium
species: Flavobacterium paronense
strain designation: KNUS1
type strain: yes
Morphology
cell morphology
- @ref: 44049
- gram stain: negative
- cell shape: rod-shaped
- motility: yes
- flagellum arrangement: gliding
colony morphology
- @ref: 44049
- colony size: 5 mm
- colony color: dark yellow
- colony shape: circular
- incubation period: 2 days
- medium used: R2A agar
pigmentation
@ref | production | name |
---|---|---|
44049 | yes | flexirubin-type |
44049 | no | brown pigments |
Culture and growth conditions
culture medium
@ref | name | growth |
---|---|---|
44049 | Blood Agar | no |
44049 | Marine agar (MA) | no |
44049 | MacConkey | no |
44049 | Nutrient agar (NA) | no |
44049 | Reasoner's 2A agar (R2A) | yes |
44049 | Trypticase Soy Agar (TSA) | no |
culture temp
@ref | growth | type | temperature |
---|---|---|---|
44049 | positive | growth | 10-30 |
44049 | positive | optimum | 25 |
culture pH
@ref | ability | type | pH |
---|---|---|---|
44049 | positive | growth | 7-8 |
44049 | positive | optimum | 8 |
Physiology and metabolism
oxygen tolerance
- @ref: 44049
- oxygen tolerance: obligate aerobe
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
44049 | NaCl | positive | growth | 0-1.0 %(w/v) |
44049 | NaCl | positive | optimum | 1 %(w/v) |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
44049 | 17128 | adipate | - | assimilation |
44049 | 27689 | decanoate | - | assimilation |
44049 | 17634 | D-glucose | - | assimilation |
44049 | 16899 | D-mannitol | - | assimilation |
44049 | 16024 | D-mannose | - | assimilation |
44049 | 30849 | L-arabinose | - | assimilation |
44049 | 25115 | malate | - | assimilation |
44049 | 17306 | maltose | - | assimilation |
44049 | 506227 | N-acetylglucosamine | - | assimilation |
44049 | 18401 | phenylacetate | - | assimilation |
44049 | 32032 | potassium gluconate | - | assimilation |
44049 | 53258 | sodium citrate | - | assimilation |
44049 | 85146 | carboxymethylcellulose | - | hydrolysis |
44049 | casein | - | hydrolysis | |
44049 | 62968 | cellulose | - | hydrolysis |
44049 | 17029 | chitin | - | hydrolysis |
44049 | 23652 | dextrin | - | hydrolysis |
44049 | 28017 | starch | - | hydrolysis |
44049 | 28053 | melibiose | + | carbon source |
44049 | 4853 | esculin | + | hydrolysis |
metabolite production
- @ref: 44049
- Chebi-ID: 16136
- metabolite: hydrogen sulfide
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
44049 | cytochrome oxidase | + | 1.9.3.1 |
44049 | catalase | + | 1.11.1.6 |
44049 | DNase | - | |
44049 | alkaline phosphatase | + | 3.1.3.1 |
44049 | esterase Lipase (C 8) | + | |
44049 | leucine arylamidase | + | 3.4.11.1 |
44049 | valine arylamidase | + | |
44049 | cystine arylamidase | + | 3.4.11.3 |
44049 | trypsin | + | 3.4.21.4 |
44049 | acid phosphatase | + | 3.1.3.2 |
44049 | naphthol-AS-BI-phosphohydrolase | + | |
44049 | esterase (C 4) | - | |
44049 | lipase (C 14) | - | |
44049 | alpha-chymotrypsin | - | 3.4.21.1 |
44049 | beta-glucuronidase | - | 3.2.1.31 |
44049 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
44049 | alpha-glucosidase | - | 3.2.1.20 |
44049 | beta-glucosidase | - | 3.2.1.21 |
44049 | alpha-mannosidase | - | 3.2.1.24 |
44049 | alpha-galactosidase | - | 3.2.1.22 |
44049 | beta-galactosidase | - | 3.2.1.23 |
44049 | alpha-fucosidase | - | 3.2.1.51 |
fatty acid profile
fatty acids
@ref fatty acid percentage 44049 C15:0 anteiso 5.4 44049 anteiso-C15:1-A 1.2 44049 C17:1 anteiso ω9c 1.2 44049 C15:1ω6c 6.8 44049 C16:0-10-methyl / iso-C17:1ω9c 5.5 44049 C16:1ω6c / C16:1ω7c 3 44049 C17:1ω6c 1.6 44049 C15:0 iso 22.9 44049 C15:0 iso 3OH 9.1 44049 iso-C15:1-G 21.1 44049 C16:0 iso 1.7 44049 iso-C16:1-H 2.3 44049 iso-C17:0-3-OH 9.7 - type of FA analysis: whole cell analysis
- incubation medium: R2A
- incubation temperature: 25
- library/peak naming table: RTSBA6
- system: MIS MIDI
- method/protocol: Sasser, 1990
- cutoff value:
Isolation, sampling and environmental information
isolation
- @ref: 44049
- sample type: freshwater near a floating vegetated island from a lake
- geographic location: Lake Paro
- country: Republic of Korea
- origin.country: KOR
- continent: Asia
- latitude: 38.0983
- longitude: 127.775
- enrichment culture: R2A
- enrichment culture duration: 2 days
- enrichment culture temperature: 20
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Freshwater |
#Environmental | #Aquatic | #Lake (large) |
Sequence information
16S sequences
- @ref: 44049
- description: Flavobacterium paronense 16S ribosomal RNA gene, partial sequence
- accession: KF499995
- database: nuccore
GC content
- @ref: 44049
- GC-content: 34.2
- method: Thermal denaturation, fluorometry
External links
@ref: 44049
culture collection no.: KACC 17692, CECT 8460
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 26530258 | Flavobacterium paronense sp. nov., isolated from freshwater of an artificial vegetated island. | Joung Y, Kang H, Kim H, Kim TS, Han JH, Kim SB, Ahn TS, Joh K | Int J Syst Evol Microbiol | 10.1099/ijsem.0.000727 | 2015 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, Fresh Water/*microbiology, Islands, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Spermidine/analogs & derivatives/chemistry, Vitamin K 2/analogs & derivatives/chemistry | Genetics |
Phylogeny | 30022265 | Flavobacterium knui sp. nov., a novel member of the family Flavobacteriaceae. | Kim SY, Ten LN, Lee SY, Kim SH, Kang IK, Jung HY | Antonie Van Leeuwenhoek | 10.1007/s10482-018-1128-3 | 2018 | Bacterial Typing Techniques, Base Composition, DNA, Bacterial/*genetics, Fatty Acids/biosynthesis, Flavobacterium/chemistry/*classification/genetics/isolation & purification, Genotype, Phenotype, Phosphatidylethanolamines/biosynthesis, *Phylogeny, RNA, Ribosomal, 16S/*genetics, Republic of Korea, Water Microbiology | Genetics |
Phylogeny | 31267336 | Flavobacterium sangjuense sp. nov. isolated from sediment. | Kim H, Heo SY, Kim JH, Chae JC, Yu SM | Antonie Van Leeuwenhoek | 10.1007/s10482-019-01297-1 | 2019 | Fatty Acids/chemistry, Flavobacterium/chemistry/*classification/*isolation & purification, Genome, Bacterial, Genomics/methods, Geologic Sediments/*microbiology, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, *Soil Microbiology | Genetics |
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
44049 | Yochan Joung, Heeyoung Kang, Haneul Kim, Tae-Su Kim, Ji-Hye Han, Seung Bum Kim, Tae-Seok Ahn and Kiseong Joh | Flavobacterium paronense sp. nov., isolated from freshwater of an artificial vegetated island | 10.1099/ijsem.0.000727 | IJSEM 66: 365-370 2016 | 26530258 |