Strain identifier

BacDive ID: 141134

Type strain: Yes

Species: Flavobacterium paronense

Strain Designation: KNUS1

NCBI tax ID(s): 1392775 (species)

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General

@ref: 44049

BacDive-ID: 141134

keywords: 16S sequence, Bacteria, obligate aerobe, Gram-negative, motile, rod-shaped, colony-forming

description: Flavobacterium paronense KNUS1 is an obligate aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from freshwater near a floating vegetated island from a lake.

NCBI tax id

  • NCBI tax id: 1392775
  • Matching level: species

doi: 10.13145/bacdive141134.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Flavobacterium
  • species: Flavobacterium paronense
  • full scientific name: Flavobacterium paronense Joung et al. 2016

@ref: 44049

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Flavobacterium

species: Flavobacterium paronense

strain designation: KNUS1

type strain: yes

Morphology

cell morphology

  • @ref: 44049
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: gliding

colony morphology

  • @ref: 44049
  • colony size: 5 mm
  • colony color: dark yellow
  • colony shape: circular
  • incubation period: 2 days
  • medium used: R2A agar

pigmentation

@refproductionname
44049yesflexirubin-type
44049nobrown pigments

Culture and growth conditions

culture medium

@refnamegrowth
44049Blood Agarno
44049Marine agar (MA)no
44049MacConkeyno
44049Nutrient agar (NA)no
44049Reasoner's 2A agar (R2A)yes
44049Trypticase Soy Agar (TSA)no

culture temp

@refgrowthtypetemperaturerange
44049positivegrowth10-30
44049positiveoptimum25mesophilic

culture pH

@refabilitytypepH
44049positivegrowth7-8
44049positiveoptimum8

Physiology and metabolism

oxygen tolerance

  • @ref: 44049
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
44049NaClpositivegrowth0-1.0 %(w/v)
44049NaClpositiveoptimum1 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4404917128adipate-assimilation
4404927689decanoate-assimilation
4404917634D-glucose-assimilation
4404916899D-mannitol-assimilation
4404916024D-mannose-assimilation
4404930849L-arabinose-assimilation
4404925115malate-assimilation
4404917306maltose-assimilation
44049506227N-acetylglucosamine-assimilation
4404918401phenylacetate-assimilation
4404932032potassium gluconate-assimilation
4404953258sodium citrate-assimilation
4404985146carboxymethylcellulose-hydrolysis
44049casein-hydrolysis
4404962968cellulose-hydrolysis
4404917029chitin-hydrolysis
4404923652dextrin-hydrolysis
4404928017starch-hydrolysis
4404928053melibiose+carbon source
440494853esculin+hydrolysis

metabolite production

  • @ref: 44049
  • Chebi-ID: 16136
  • metabolite: hydrogen sulfide
  • production: no

enzymes

@refvalueactivityec
44049cytochrome oxidase+1.9.3.1
44049catalase+1.11.1.6
44049DNase-
44049alkaline phosphatase+3.1.3.1
44049esterase Lipase (C 8)+
44049leucine arylamidase+3.4.11.1
44049valine arylamidase+
44049cystine arylamidase+3.4.11.3
44049trypsin+3.4.21.4
44049acid phosphatase+3.1.3.2
44049naphthol-AS-BI-phosphohydrolase+
44049esterase (C 4)-
44049lipase (C 14)-
44049alpha-chymotrypsin-3.4.21.1
44049beta-glucuronidase-3.2.1.31
44049N-acetyl-beta-glucosaminidase-3.2.1.52
44049alpha-glucosidase-3.2.1.20
44049beta-glucosidase-3.2.1.21
44049alpha-mannosidase-3.2.1.24
44049alpha-galactosidase-3.2.1.22
44049beta-galactosidase-3.2.1.23
44049alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    44049C15:0 anteiso5.4
    44049anteiso-C15:1-A1.2
    44049C17:1 anteiso ω9c1.2
    44049C15:1ω6c6.8
    44049C16:0-10-methyl / iso-C17:1ω9c5.5
    44049C16:1ω6c / C16:1ω7c3
    44049C17:1ω6c1.6
    44049C15:0 iso22.9
    44049C15:0 iso 3OH9.1
    44049iso-C15:1-G21.1
    44049C16:0 iso1.7
    44049iso-C16:1-H2.3
    44049iso-C17:0-3-OH9.7
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • incubation temperature: 25
  • library/peak naming table: RTSBA6
  • system: MIS MIDI
  • method/protocol: Sasser, 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 44049
  • sample type: freshwater near a floating vegetated island from a lake
  • geographic location: Lake Paro
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia
  • latitude: 38.0983
  • longitude: 127.775
  • enrichment culture: R2A
  • enrichment culture duration: 2 days
  • enrichment culture temperature: 20

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Freshwater
#Environmental#Aquatic#Lake (large)

Sequence information

16S sequences

  • @ref: 44049
  • description: 16S rRNA gene sequence
  • accession: KF499995
  • database: nuccore

GC content

  • @ref: 44049
  • GC-content: 34.2
  • method: Thermal denaturation, fluorometry

External links

@ref: 44049

culture collection no.: KACC 17692, CECT 8460

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny26530258Flavobacterium paronense sp. nov., isolated from freshwater of an artificial vegetated island.Joung Y, Kang H, Kim H, Kim TS, Han JH, Kim SB, Ahn TS, Joh KInt J Syst Evol Microbiol10.1099/ijsem.0.0007272015Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, Fresh Water/*microbiology, Islands, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Spermidine/analogs & derivatives/chemistry, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny30022265Flavobacterium knui sp. nov., a novel member of the family Flavobacteriaceae.Kim SY, Ten LN, Lee SY, Kim SH, Kang IK, Jung HYAntonie Van Leeuwenhoek10.1007/s10482-018-1128-32018Bacterial Typing Techniques, Base Composition, DNA, Bacterial/*genetics, Fatty Acids/biosynthesis, Flavobacterium/chemistry/*classification/genetics/isolation & purification, Genotype, Phenotype, Phosphatidylethanolamines/biosynthesis, *Phylogeny, RNA, Ribosomal, 16S/*genetics, Republic of Korea, Water MicrobiologyGenetics
Phylogeny31267336Flavobacterium sangjuense sp. nov. isolated from sediment.Kim H, Heo SY, Kim JH, Chae JC, Yu SMAntonie Van Leeuwenhoek10.1007/s10482-019-01297-12019Fatty Acids/chemistry, Flavobacterium/chemistry/*classification/*isolation & purification, Genome, Bacterial, Genomics/methods, Geologic Sediments/*microbiology, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, *Soil MicrobiologyGenetics

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
44049Yochan Joung, Heeyoung Kang, Haneul Kim, Tae-Su Kim, Ji-Hye Han, Seung Bum Kim, Tae-Seok Ahn and Kiseong JohFlavobacterium paronense sp. nov., isolated from freshwater of an artificial vegetated island10.1099/ijsem.0.000727IJSEM 66: 365-370 201626530258