Strain identifier

BacDive ID: 141094

Type strain: Yes

Species: Roseomonas eburnea

Strain Designation: BUT-5

NCBI tax ID(s): 1346889 (species)

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General

@ref: 44000

BacDive-ID: 141094

keywords: 16S sequence, Bacteria, Gram-negative, rod-shaped, colony-forming

description: Roseomonas eburnea BUT-5 is a Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from activated sludge of an herbicides-manufacturing wastewater treatment facility.

NCBI tax id

  • NCBI tax id: 1346889
  • Matching level: species

doi: 10.13145/bacdive141094.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodospirillales
  • family: Acetobacteraceae
  • genus: Roseomonas
  • species: Roseomonas eburnea
  • full scientific name: Roseomonas eburnea Wang et al. 2016

  • synonyms

    • @ref: 20215
    • synonym: Neoroseomonas eburnea

@ref: 44000

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodospirillales

family: Acetobacteraceae

genus: Roseomonas

species: Roseomonas eburnea

strain designation: BUT-5

type strain: yes

Morphology

cell morphology

  • @ref: 44000
  • gram stain: negative
  • cell length: 1.3-2.3 µm
  • cell width: 0.6-0.9 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 44000
  • colony size: 2 mm
  • colony color: ivory-pigmented, shiny
  • colony shape: circular
  • incubation period: 5 days
  • medium used: R2A agar

Culture and growth conditions

culture medium

  • @ref: 44000
  • name: Reasoners 2A agar
  • growth: yes

culture temp

@refgrowthtypetemperature
44000positivegrowth15-35
44000positiveoptimum30

culture pH

@refabilitytypepHPH range
44000positivegrowth3.0-10.0alkaliphile
44000positiveoptimum6.0-8.0

Physiology and metabolism

spore formation

  • @ref: 44000
  • type of spore: endospore
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
44000NaClpositivegrowth0.5-7.0 %
44000NaClpositiveoptimum1.0-2.0 %

observation

  • @ref: 44000
  • observation: predominant respiratory quinone is ubiquinone Q-10

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4400016947citrate-assimilation
4400027613amygdalin-builds acid from
4400017634D-glucose-builds acid from
4400016899D-mannitol-builds acid from
4400017924D-sorbitol-builds acid from
4400062345L-rhamnose-builds acid from
4400017716lactose-builds acid from
4400017306maltose-builds acid from
4400028053melibiose-builds acid from
4400017268myo-inositol-builds acid from
4400017992sucrose-builds acid from
44000esculin ferric citrate-hydrolysis
440005291gelatin-hydrolysis
4400017368hypoxanthine-hydrolysis
4400028017starch-hydrolysis
4400053424tween 20-hydrolysis
4400053426tween 80-hydrolysis
4400018186tyrosine-hydrolysis
4400016199urea-hydrolysis
4400017632nitrate-reduction
44000286442-oxopentanoate+carbon source
44000181014-hydroxyphenylacetic acid+carbon source
4400017128adipate+carbon source
440008295beta-hydroxybutyrate+carbon source
4400027689decanoate+carbon source
4400015570D-alanine+carbon source
4400024996lactate+carbon source
4400025115malate+carbon source
4400021217L-alaninamide+carbon source
4400073786L-alanylglycine+carbon source
4400017196L-asparagine+carbon source
4400015603L-leucine+carbon source
4400051850methyl pyruvate+carbon source
4400075146monomethyl succinate+carbon source
4400017272propionate+carbon source
4400064243sodium L-glutamate+carbon source
44000casein+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.
4400045924trimethoprimyesyes5 µg (disc)
44000157175enoxacinyesyes10 µg (disc)

metabolite production

@refChebi-IDmetaboliteproduction
4400016136hydrogen sulfideno
4400030854indole-3-acetateno

enzymes

@refvalueactivityec
44000catalase+1.11.1.6
44000cytochrome oxidase-1.9.3.1
44000beta-galactosidase-3.2.1.23
44000arginine dihydrolase-3.5.3.6
44000lysine decarboxylase-4.1.1.18
44000cystine arylamidase+3.4.11.3
44000chymotrypsin+3.4.4.5
44000alpha-galactosidase+3.2.1.22
44000alpha-glucosidase+3.2.1.20
44000valine arylamidase+
44000alpha-mannosidase+3.2.1.24
44000alkaline phosphatase-3.1.3.1
44000esterase (C 4)-
44000naphthol-AS-BI-phosphohydrolase-

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    44000C16:07.9
    44000C16:0 2OH1.4
    44000C16:0 3OH0.9
    44000C16:1ω5c3.1
    44000C17:1ω7c1.2
    44000C18:02
    44000C18:1 2OH6.7
    44000C18:1ω5c1
    4400011-methyl C18:1ω7c0.2
    44000C18:1ω9c1.2
    44000C19:0cycloω8c2.3
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • agar/liquid: liquid
  • incubation temperature: 30
  • system: MIS MIDI
  • method/protocol: Sasser, 1990

Isolation, sampling and environmental information

isolation

  • @ref: 44000
  • sample type: activated sludge of an herbicides-manufacturing wastewater treatment facility
  • geographic location: Kunshan city, Jiangsu province
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 31.3666
  • longitude: 120.933

isolation source categories

Cat1Cat2Cat3
#Engineered#Waste#Activated sludge
#Engineered#Waste#Water treatment plant

taxonmaps

  • @ref: 69479
  • File name: preview.99_143794.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_266;96_5468;97_6593;98_17518;99_143794&stattab=map
  • Last taxonomy: Roseomonas eburnea
  • 16S sequence: KF254767
  • Sequence Identity:
  • Total samples: 243
  • soil counts: 72
  • aquatic counts: 127
  • animal counts: 34
  • plant counts: 10

Sequence information

16S sequences

  • @ref: 44000
  • description: Neoroseomonas eburnea 16S ribosomal RNA gene, partial sequence
  • accession: KF254767
  • database: nuccore

GC content

  • @ref: 44000
  • GC-content: 67.6

External links

@ref: 44000

culture collection no.: CCTCC AB 2013276, KACC 17166

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny26530339Roseomonas eburnea sp. nov., isolated from activated sludge.Wang C, Deng S, Liu X, Yao L, Shi C, Jiang J, Kwon SW, He J, Li JInt J Syst Evol Microbiol10.1099/ijsem.0.0007282015Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Methylobacteriaceae/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Ubiquinone/analogs & derivatives/chemistry, Waste Water/microbiologyGenetics
Phylogeny35171088Roseomonas rubea sp. nov., isolated from rice paddy soil.Lee HJ, Whang KSInt J Syst Evol Microbiol10.1099/ijsem.0.0052512022Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Methylobacteriaceae/classification/isolation & purification, *Oryza, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
44000Chenghong Wang, Shikai Deng, Xin Liu, Li Yao, Chao Shi, Jin Jiang, Soon-Wo Kwon, Jian He, Jiayou LiRoseomonas eburnea sp. nov., isolated from activated sludge10.1099/ijsem.0.000728IJSEM 66: 385-390 201626530339
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/