Strain identifier

BacDive ID: 141089

Type strain: Yes

Species: Cohnella saccharovorans

Strain Designation: CJ22

Strain history: C.-J. Cha CJ22.

NCBI tax ID(s): 1255256 (species)

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General

@ref: 43994

BacDive-ID: 141089

keywords: 16S sequence, Bacteria, aerobe, Gram-positive, motile, rod-shaped, colony-forming

description: Cohnella saccharovorans CJ22 is an aerobe, Gram-positive, motile bacterium that forms circular colonies and was isolated from soil of a ginseng field located in Anseong, Korea.

NCBI tax id

  • NCBI tax id: 1255256
  • Matching level: species

strain history

  • @ref: 67770
  • history: C.-J. Cha CJ22.

doi: 10.13145/bacdive141089.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Cohnella
  • species: Cohnella saccharovorans
  • full scientific name: Cohnella saccharovorans Choi et al. 2016

@ref: 43994

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Paenibacillaceae

genus: Cohnella

species: Cohnella saccharovorans

strain designation: CJ22

type strain: yes

Morphology

cell morphology

  • @ref: 43994
  • gram stain: positive
  • cell length: 2 µm
  • cell width: 0.5 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: peritrichous

colony morphology

  • @ref: 43994
  • colony color: creamy white
  • colony shape: circular
  • medium used: R2A agar

Culture and growth conditions

culture medium

  • @ref: 43994
  • name: Reasoner's 2A agar (R2A)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43994positivegrowth20-37
43994positiveoptimum30mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepH
43994positivegrowth6.0-8.0
43994positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 43994
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43994
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
43994NaClpositivegrowth0-1 %(w/v)
43994NaClpositiveoptimum0 %(w/v)

observation

@refobservation
43994quinone MK-7
67770quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
43994168082-dehydro-D-gluconate-builds acid from
4399417128adipate-builds acid from
4399422599arabinose-builds acid from
4399429016arginine-builds acid from
4399427689decanoate-builds acid from
4399415963ribitol-builds acid from
4399418333D-arabitol-builds acid from
4399428847D-fucose-builds acid from
4399416899D-mannitol-builds acid from
4399417924D-sorbitol-builds acid from
4399416443D-tagatose-builds acid from
4399417113erythritol-builds acid from
4399424265gluconate-builds acid from
4399417234glucose-builds acid from
4399415978glycerol 3-phosphate-builds acid from
4399417268myo-inositol-builds acid from
4399418403L-arabitol-builds acid from
4399417266L-sorbose-builds acid from
4399465328L-xylose-builds acid from
4399418300maleic acid-builds acid from
4399417306maltose-builds acid from
4399429864mannitol-builds acid from
4399437684mannose-builds acid from
4399418401phenylacetate-builds acid from
4399417151xylitol-builds acid from
43994casein-hydrolysis
4399416991dna-hydrolysis
439945291gelatin-hydrolysis
4399417632nitrate-reduction
43994581435-dehydro-D-gluconate+builds acid from
4399427613amygdalin+builds acid from
4399418305arbutin+builds acid from
4399417057cellobiose+builds acid from
4399417108D-arabinose+builds acid from
4399415824D-fructose+builds acid from
4399412936D-galactose+builds acid from
4399417634D-glucose+builds acid from
4399462318D-lyxose+builds acid from
4399416024D-mannose+builds acid from
4399416988D-ribose+builds acid from
4399465327D-xylose+builds acid from
4399416813galactitol+builds acid from
4399428066gentiobiose+builds acid from
4399428087glycogen+builds acid from
4399415443inulin+builds acid from
4399430849L-arabinose+builds acid from
4399418287L-fucose+builds acid from
4399462345L-rhamnose+builds acid from
4399417716lactose+builds acid from
4399417306maltose+builds acid from
439946731melezitose+builds acid from
4399428053melibiose+builds acid from
43994320061methyl alpha-D-glucopyranoside+builds acid from
4399443943methyl alpha-D-mannoside+builds acid from
4399474863methyl beta-D-xylopyranoside+builds acid from
43994506227N-acetylglucosamine+builds acid from
4399416634raffinose+builds acid from
4399417814salicin+builds acid from
4399417992sucrose+builds acid from
4399427082trehalose+builds acid from
4399432528turanose+builds acid from
439944853esculin+hydrolysis
4399428017starch+hydrolysis
4399416199urea+hydrolysis

metabolite production

  • @ref: 43994
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
43994catalase+1.11.1.6
43994cytochrome oxidase+1.9.3.1
439942-nitrophenyl beta-D-galactopyranoside+3.2.1.52
43994esterase (C 4)+
43994esterase Lipase (C 8)+
43994leucine arylamidase+3.4.11.1
43994alpha-galactosidase+3.2.1.22
43994beta-galactosidase+3.2.1.23
43994alpha-glucosidase+3.2.1.20
43994beta-glucosidase+3.2.1.21
43994alkaline phosphatase-3.1.3.1
43994lipase (C 14)-
43994valine arylamidase-
43994cystine arylamidase-3.4.11.3
43994trypsin-3.4.21.4
43994alpha-chymotrypsin-3.4.21.1
43994acid phosphatase-3.1.3.2
43994naphthol-AS-BI-phosphohydrolase-
43994beta-glucuronidase-3.2.1.31
43994N-acetyl-beta-glucosaminidase-3.2.1.52
43994alpha-mannosidase-3.2.1.24
43994alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43994C15:0 anteiso42
    43994C17:0 anteiso4.8
    43994C14:02.3
    43994C16:011.2
    43994C14:0 iso4.5
    43994C15:0 iso2.3
    43994C16:0 iso30.2
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A agar
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 3
  • software version: Sherlock 6.1
  • library/peak naming table: RTSBA6
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperature
43994soil of a ginseng field located in Anseong, KoreaAnseongRepublic of KoreaKORAsiaR2A agar3 days30
67770Soil of a ginseng field located in AnseongRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Field
#Environmental#Terrestrial#Soil

Sequence information

16S sequences

  • @ref: 43994
  • description: 16S rRNA gene sequence
  • accession: JQ806733
  • database: nuccore

GC content

  • @ref: 67770
  • GC-content: 63.1
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43994

culture collection no.: KACC 17501, JCM 19227

literature

  • topic: Phylogeny
  • Pubmed-ID: 26813106
  • title: Cohnella saccharovorans sp. nov., isolated from ginseng soil.
  • authors: Choi JH, Seok JH, Jang HJ, Cha JH, Cha CJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000933
  • year: 2016
  • mesh: Bacillales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Panax/*microbiology, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Genetics

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43994Jung-Hye Choi, Ji-Hye Seok, Ho-Jin Jang, Ju-Hee Cha, Chang-Jun ChaCohnella saccharovorans sp. nov., isolated from ginseng soil10.1099/ijsem.0.000933IJSEM 66: 1887-1893 201626813106
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/