Strain identifier

BacDive ID: 141055

Type strain: Yes

Species: Actibacterium ureilyticum

Strain Designation: LS-811

Strain history: L.-S. Young; Natl. Formosa Univ., Taiwan; LS-811.

NCBI tax ID(s): 1590614 (species)

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General

@ref: 43935

BacDive-ID: 141055

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped, colony-forming

description: Actibacterium ureilyticum LS-811 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from seawater of the South China Sea, Taiwan.

NCBI tax id

  • NCBI tax id: 1590614
  • Matching level: species

strain history

  • @ref: 67770
  • history: L.-S. Young; Natl. Formosa Univ., Taiwan; LS-811.

doi: 10.13145/bacdive141055.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Roseobacteraceae
  • genus: Actibacterium
  • species: Actibacterium ureilyticum
  • full scientific name: Actibacterium ureilyticum Lin et al. 2016

@ref: 43935

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Actibacterium

species: Actibacterium ureilyticum

strain designation: LS-811

type strain: yes

Morphology

cell morphology

  • @ref: 43935
  • gram stain: negative
  • cell length: 1.5-1.9 µm
  • cell width: 0.6-0.8 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: monotrichous

colony morphology

  • @ref: 43935
  • colony size: 1-2 mm
  • colony color: white
  • colony shape: circular
  • incubation period: 2 days
  • medium used: Marine agar 2216

Culture and growth conditions

culture medium

  • @ref: 43935
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43935positivegrowth15-40
43935positiveoptimum30mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
43935positivegrowth5.0-9.0alkaliphile
43935positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 43935
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
43935NaClpositivegrowth1-6 %(w/v)
43935NaClpositiveoptimum3 %(w/v)

observation

@refobservation
43935quinone Q-10
67770quinones: Q-10

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4393517306maltose-carbon source
4393517634D-glucose+assimilation
43935167632-oxobutanoate+carbon source
43935309162-oxoglutarate+carbon source
43935286442-oxopentanoate+carbon source
4393517925alpha-D-glucose+carbon source
43935645522-hydroxybutyrate+carbon source
4393536219alpha-lactose+carbon source
439358295beta-hydroxybutyrate+carbon source
4393516383cis-aconitate+carbon source
4393516947citrate+carbon source
4393515570D-alanine+carbon source
4393515824D-fructose+carbon source
4393512936D-galactose+carbon source
439358391D-gluconate+carbon source
4393517784D-glucosaminic acid+carbon source
4393515748D-glucuronate+carbon source
4393516899D-mannitol+carbon source
4393517924D-sorbitol+carbon source
4393523652dextrin+carbon source
4393517113erythritol+carbon source
4393515740formate+carbon source
4393528066gentiobiose+carbon source
4393528087glycogen+carbon source
4393517596inosine+carbon source
4393517240itaconate+carbon source
4393521217L-alaninamide+carbon source
4393516977L-alanine+carbon source
4393517196L-asparagine+carbon source
4393515603L-leucine+carbon source
4393517295L-phenylalanine+carbon source
4393562345L-rhamnose+carbon source
4393517115L-serine+carbon source
439356359lactulose+carbon source
43935320055methyl beta-D-glucopyranoside+carbon source
4393551850methyl pyruvate+carbon source
4393517268myo-inositol+carbon source
4393528037N-acetylgalactosamine+carbon source
43935506227N-acetylglucosamine+carbon source
43935143136succinamate+carbon source
4393527082trehalose+carbon source
4393532528turanose+carbon source
4393553423tween 40+carbon source
4393553426tween 80+carbon source
4393517151xylitol+carbon source
4393517632nitrate+reduction
439355291gelatin+/-hydrolysis

enzymes

@refvalueactivityec
43935catalase+1.11.1.6
43935cytochrome oxidase+1.9.3.1
43935urease+3.5.1.5
43935protease+
43935acid phosphatase+3.1.3.2
43935alkaline phosphatase+3.1.3.1
43935esterase (C 4)+
43935esterase Lipase (C 8)+
43935leucine arylamidase+3.4.11.1
43935valine arylamidase+
43935cystine arylamidase+3.4.11.3
43935naphthol-AS-BI-phosphohydrolase+
43935beta-glucosidase+3.2.1.21
43935alpha-mannosidase+3.2.1.24
43935beta-glucosidase-3.2.1.21
43935beta-galactosidase-3.2.1.23

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43935C13:0 anteiso0.1
    43935C10:0 3OH6.9
    43935C12:00.5
    43935C16:01.1
    43935C16:1ω7c / C16:1ω6c0.5
    43935C17:00.6
    43935C17:1ω6c0.5
    43935C18:06
    43935C18:1ω7c / C18:1ω6c66.4
    4393510-methyl C18:1ω7c1.3
    43935C19:0ω8c cyclo11.9
    43935C19:1ω6c,ω7c / 19cy0.9
    43935C20:1ω7c0.8
    43935C20:2ω6c, ω9c0.5
    43935C11:0 iso 3OH0.2
    43935C15:0 iso0.2
    43935C17:1 iso ω10c0.5
    43935C19:0 iso0.2
  • type of FA analysis: whole cell analysis
  • incubation medium: Marine agar 2216
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 2
  • software version: Sherlock 6.0
  • library/peak naming table: RTSBA6
  • system: MIS MIDI
  • instrument: Agilent 7890A gas chromatogrpah
  • method/protocol: Sasser, 1990

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperature
43935seawater of the South China Sea, TaiwanSouth China SeaTaiwanTWNAsia21.6035118.193Marine agar 22163 days30
67770Seawater of the South China SeaTaiwanTWNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_6210.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_19;96_2958;97_3639;98_4605;99_6210&stattab=map
  • Last taxonomy: Actibacterium ureilyticum subclade
  • 16S sequence: KP162062
  • Sequence Identity:
  • Total samples: 2641
  • soil counts: 106
  • aquatic counts: 2282
  • animal counts: 202
  • plant counts: 51

Sequence information

16S sequences

  • @ref: 43935
  • description: 16S rRNA gene sequence
  • accession: KP162062
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actibacterium ureilyticum LS-8112832212542draftimg1590614
67770Actibacterium ureilyticum LS-811GCA_002285435contigncbi1590614

GC content

  • @ref: 67770
  • GC-content: 60.1
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43935

culture collection no.: BCRC 80823, JCM 30681

literature

  • topic: Phylogeny
  • Pubmed-ID: 27031530
  • title: Actibacterium ureilyticum sp. nov., isolated from seawater.
  • authors: Lin SY, Young CC, Hameed A, Liu YC, Hsu YH, Chung WC, Young LS
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001052
  • year: 2016
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Taiwan, Ubiquinone/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43935Shih-Yao Lin, Chiu-Chung Young, Asif Hameed, You-Cheng Liu, Yi-Han Hsu, Wei-Ching Chung, Li-Sen YoungActibacterium ureilyticum sp. nov., isolated from seawater10.1099/ijsem.0.001052IJSEM 66: 2769-2773 201627031530
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/