Strain identifier

BacDive ID: 141032

Type strain: Yes

Species: Butyratibacter algicola

Strain Designation: B15

NCBI tax ID(s): 2029869 (species)

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General

@ref: 43914

BacDive-ID: 141032

keywords: 16S sequence, Bacteria, psychrophilic, Gram-negative, motile, ovoid-shaped, colony-forming

description: Butyratibacter algicola B15 is a psychrophilic, Gram-negative, motile bacterium that forms circular colonies and was isolated from liquid subcultures of marine microalga Picochlorum sp.122.

NCBI tax id

  • NCBI tax id: 2029869
  • Matching level: species

doi: 10.13145/bacdive141032.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Tepidamorphaceae
  • genus: Butyratibacter
  • species: Butyratibacter algicola
  • full scientific name: Butyratibacter algicola Wang et al. 2017

@ref: 43914

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Hyphomicrobiales

family: Rhodobiaceae

genus: Butyratibacter

species: Butyratibacter algicola

strain designation: B15

type strain: yes

Morphology

cell morphology

  • @ref: 43914
  • gram stain: negative
  • cell length: 0.4-1.5 µm
  • cell width: 0.4-0.6 µm
  • cell shape: ovoid-shaped
  • motility: yes

colony morphology

  • @ref: 43914
  • colony color: white or colourless
  • colony shape: circular
  • medium used: Butyrate enhanced R2A

Culture and growth conditions

culture medium

@refnamegrowthcomposition
43914butyrate enhanced R2Ayessupplemented with 0.3 % (w/v) butyrate
43914pyruvate-enhanced Glucose-Yeast-Peptoneglycerol 3 ml/L, yeast extract 1 g/L, tryptone 2 g/L, pyruvate 0.3 g/L, agar powder 18 g/L and aged seawater 1 L, pH 8.0

culture temp

@refgrowthtypetemperaturerange
43914positivegrowth15psychrophilic
43914positivegrowth20psychrophilic
43914positivegrowth25mesophilic
43914positivegrowth33mesophilic
43914positivegrowth37mesophilic
43914positiveoptimum33mesophilic

culture pH

@refabilitytypepHPH range
43914positivegrowth7.0-10.0alkaliphile
43914positiveoptimum8.0-9.0

Physiology and metabolism

spore formation

  • @ref: 43914
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
43914NaClpositivegrowth0.5-7 %(w/v)
43914NaClpositiveoptimum3 %(w/v)

observation

  • @ref: 43914
  • observation: quinone Q-10

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4391428757fructose-carbon source
4391417234glucose-carbon source
439145291gelatin-degradation
439145291gelatin-hydrolysis
4391428017starch-hydrolysis
4391453424tween 20-hydrolysis
4391453423tween 40-hydrolysis
4391453426tween 80-hydrolysis
4391427689decanoate+carbon source
4391416947citrate+carbon source
4391424265gluconate+carbon source
4391425115malate+carbon source
43914506227N-acetylglucosamine+carbon source
4391430089acetate+oxidation
4391416865gamma-aminobutyric acid+oxidation
4391429991L-aspartate+oxidation
4391451850methyl pyruvate+oxidation
4391464103sodium butyrate+oxidation
4391475228sodium lactate+oxidation

metabolite production

@refChebi-IDmetaboliteproduction
4391416136hydrogen sulfideno
4391435581indoleno

enzymes

@refvalueactivityec
43914catalase+1.11.1.6
43914nitrate reductase+1.7.99.4
43914cytochrome oxidase-1.9.3.1
43914urease+3.5.1.5
43914arginine dihydrolase+3.5.3.6

Isolation, sampling and environmental information

isolation

  • @ref: 43914
  • sample type: liquid subcultures of marine microalga Picochlorum sp.122
  • enrichment culture: pyruvate-enhanced Glucose-Yeast-Peptone
  • enrichment culture composition: glycerol 3 ml/L, yeast extract 1 g/L, tryptone 2 g/L, pyruvate 0.3 g/L, agar powder 18 g/L and aged seawater 1 L, pH 8.0
  • enrichment culture duration: 15 days
  • enrichment culture temperature: 25

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Host#Algae

Sequence information

16S sequences

  • @ref: 43914
  • description: 16S rRNA gene sequence
  • accession: KX989462
  • database: nuccore

GC content

  • @ref: 43914
  • GC-content: 66.3
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43914

culture collection no.: KCTC 52552, MCCC 1K03221

literature

  • topic: Phylogeny
  • Pubmed-ID: 28829015
  • title: Butyratibacter algicola gen. nov., sp. nov., a marine bacterium from the culture broth of Picochlorum sp. 122.
  • authors: Wang G, Wang Y, Su H, Wu H, Li T, Liang J, Huang W, Xiang W, Yu K
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002081
  • year: 2017
  • mesh: Alphaproteobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Chlorophyta/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43914Guanghua Wang, Yinghui Wang, Hongfei Su, Hualian Wu, Tao Li, Jiayuan Liang, Wen Huang, Wenzhou Xiang, Kefu YuButyratibacter algicola gen. nov., sp. nov., a marine bacterium from the culture broth of Picochlorum sp. 12210.1099/ijsem.0.002081IJSEM 67: 3209-3213 201728829015