Strain identifier
BacDive ID: 140938
Type strain: 
Species: Thermostilla marina
Strain Designation: SVX8
Strain history: G. B. Slobodkina; Winogradsky Inst. of Microbiol., RAS, Russia; SVX8.
NCBI tax ID(s): 1742923 (species)
version 8.1 (current version)
General
@ref: 43826
BacDive-ID: 140938
keywords: 16S sequence, Bacteria, facultative anaerobe, chemoorganotroph, motile, coccus-shaped
description: Thermostilla marina SVX8 is a facultative anaerobe, chemoorganotroph, motile bacterium that was isolated from A mixed sample of sand, fluid and seawater collected at a shallow submarine hydrothermal vent at a depth of 0.5 m and a 3 m offshore.
NCBI tax id
- NCBI tax id: 1742923
- Matching level: species
strain history
- @ref: 67770
- history: G. B. Slobodkina; Winogradsky Inst. of Microbiol., RAS, Russia; SVX8.
doi: 10.13145/bacdive140938.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/planctomycetota
- domain: Bacteria
- phylum: Planctomycetota
- class: Planctomycetia
- order: Pirellulales
- family: Thermoguttaceae
- genus: Thermostilla
- species: Thermostilla marina
- full scientific name: Thermostilla marina Slobodkina et al. 2016
@ref: 43826
domain: Bacteria
phylum: Planctomycetes
class: Planctomycea
order: Pirellulales
family: Planctomycetaceae
genus: Thermostilla
species: Thermostilla marina
strain designation: SVX8
type strain: yes
Morphology
cell morphology
- @ref: 43826
- cell length: 0.5-1.2 µm
- cell shape: coccus-shaped
- motility: yes
- flagellum arrangement: monotrichous, polar
Culture and growth conditions
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 43826 | positive | growth | 30-68 | |
| 43826 | positive | optimum | 55 | thermophilic |
| 67770 | positive | growth | 50 | thermophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 43826 | positive | growth | 5-9 | alkaliphile |
| 43826 | positive | optimum | 7-8 |
Physiology and metabolism
oxygen tolerance
- @ref: 43826
- oxygen tolerance: facultative anaerobe
nutrition type
- @ref: 43826
- type: chemoorganotroph
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 43826 | NaCl | positive | growth | 0.8-4.5 %(w/v) |
| 43826 | NaCl | positive | optimum | 2.5-3.5 %(w/v) |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 43826 | 17045 | dinitrogen oxide | - | electron acceptor |
| 43826 | 28757 | fructose | - | electron donor |
| 43826 | 28260 | galactose | - | electron donor |
| 43826 | 24996 | lactate | - | electron donor |
| 43826 | 17716 | lactose | - | electron donor |
| 43826 | 37684 | mannose | - | electron donor |
| 43826 | 18222 | xylose | - | electron donor |
| 43826 | 33403 | elemental sulfur | + | electron acceptor |
| 43826 | 2511 | agarose | + | electron donor |
| 43826 | 22599 | arabinose | + | electron donor |
| 43826 | 58187 | alginate | +/- | electron donor |
| 43826 | 15361 | pyruvate | +/- | electron donor |
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 43826 | catalase | + | 1.11.1.6 |
| 43826 | cytochrome oxidase | - | 1.9.3.1 |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 43826 | A mixed sample of sand, fluid (thermal water and dissolved gases) and seawater collected at a shallow submarine hydrothermal vent (70° C, pH 6.5-7) at a depth of 0.5 m and a 3 m offshore | Vulcano Island | Italy | ITA | Europe |
| 67770 | Shallow submarine hydrothermal vent | Vulcano Island | Italy | ITA | Europe |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Condition | #Thermophilic (>45°C) | |
| #Environmental | #Aquatic | #Hydrothermal vent |
| #Environmental | #Aquatic | #Marine |
| #Environmental | #Terrestrial | #Sandy |
Sequence information
16S sequences
- @ref: 43826
- description: 16S rRNA gene sequence
- accession: KR872395
- database: nuccore
GC content
- @ref: 67770
- GC-content: 58.5
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 43826
culture collection no.: JCM 19992, VKM B-2881
literature
- topic: Phylogeny
- Pubmed-ID: 26559645
- title: Thermostilla marina gen. nov., sp. nov., a thermophilic, facultatively anaerobic planctomycete isolated from a shallow submarine hydrothermal vent.
- authors: Slobodkina GB, Panteleeva AN, Beskorovaynaya DA, Bonch-Osmolovskaya EA, Slobodkin AI
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.000767
- year: 2015
- mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hydrothermal Vents/*microbiology, Italy, Nitrification, *Phylogeny, Planctomycetales/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Sequence Analysis, DNA, Sulfur
- topic2: Transcriptome
Reference
| @id | authors | title | doi/url | journal | pubmed |
|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
| 43826 | Galina B. Slobodkina, Angela N. Panteleeva, Darya A. Beskorovaynaya, Elizaveta A. Bonch-Osmolovskaya, Alexander I. Slobodkin | Thermostilla marina gen. nov., sp. nov., a thermophilic, facultatively anaerobic planctomycete isolated from a shallow submarine hydrothermal vent | 10.1099/ijsem.0.000767 | IJSEM 66: 633-638 2016 | 26559645 |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ |