Strain identifier

BacDive ID: 140904

Type strain: Yes

Species: Pseudomonas populi

Strain Designation: KBL-4-9

NCBI tax ID(s): 1500966 (species)

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For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 43748

BacDive-ID: 140904

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Pseudomonas populi KBL-4-9 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from Populus euphratica sample at the ancient Khiyik River.

NCBI tax id

  • NCBI tax id: 1500966
  • Matching level: species

doi: 10.13145/bacdive140904.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas populi
  • full scientific name: Pseudomonas populi Anwar et al. 2016

@ref: 43748

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas populi

strain designation: KBL-4-9

type strain: yes

Morphology

cell morphology

  • @ref: 43748
  • gram stain: negative
  • cell length: 0.5-1.6 µm
  • cell width: 0.5-0.6 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 43748
  • colony size: 1-1.5 mm
  • colony color: Creamy
  • colony shape: circular
  • incubation period: 3 days
  • medium used: Luria Bertani agar

Culture and growth conditions

culture medium

  • @ref: 43748
  • name: LB (Luria-Bertani) MEDIUM
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43748positivegrowth4-45
43748positiveoptimum37mesophilic

culture pH

@refabilitytypepHPH range
43748positivegrowth5.5-9.5alkaliphile
43748positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 43748
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
43748NaClpositivegrowth1-3 %(w/v)
43748NaClpositiveoptimum1 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4374817634D-glucose-assimilation
4374816899D-mannitol-assimilation
4374816024D-mannose-assimilation
4374830849L-arabinose-assimilation
4374848607lithium chloride-assimilation
4374825115malate-assimilation
4374817306maltose-assimilation
43748506227N-acetylglucosamine-assimilation
4374818401phenylacetate-assimilation
4374832032potassium gluconate-assimilation
4374853258sodium citrate-assimilation
4374817234glucose-fermentation
4374858187alginate-hydrolysis
43748casein-hydrolysis
4374816991dna-hydrolysis
437484853esculin-hydrolysis
437485291gelatin-hydrolysis
4374828017starch-hydrolysis
4374817968butyrate-respiration
4374817057cellobiose-respiration
4374818333D-arabitol-respiration
4374829990D-aspartate-respiration
4374812936D-galactose-respiration
4374816899D-mannitol-respiration
4374816024D-mannose-respiration
4374816523D-serine-respiration
4374817924D-sorbitol-respiration
4374823652dextrin-respiration
4374871321fusidate-respiration
4374828066gentiobiose-respiration
4374817754glycerol-respiration
4374818287L-fucose-respiration
4374862345L-rhamnose-respiration
4374817306maltose-respiration
4374828053melibiose-respiration
4374874611methyl (R)-lactate-respiration
4374817268myo-inositol-respiration
4374816634raffinose-respiration
4374817164stachyose-respiration
4374817992sucrose-respiration
4374827082trehalose-respiration
4374832528turanose-respiration
4374817128adipate+assimilation
4374827689decanoate+assimilation
4374850694minocycline+assimilation
4374863043potassium nitrate+assimilation
4374875248potassium tellurite+assimilation
4374853426tween 80+hydrolysis
4374816651(S)-lactate+respiration
4374815589L-malate+respiration
43748309162-oxoglutarate+respiration
4374830089acetate+respiration
4374813705acetoacetate+respiration
4374873706bromosuccinate+respiration
4374816947citrate+respiration
4374815824D-fructose+respiration
4374828847D-fucose+respiration
4374815895D-galactonic acid lactone+respiration
4374818024D-galacturonic acid+respiration
437488391D-gluconate+respiration
4374815748D-glucuronate+respiration
4374833801D-saccharate+respiration
4374815740formate+respiration
4374816537galactarate+respiration
4374816865gamma-aminobutyric acid+respiration
4374832323glucuronamide+respiration
4374870744glycine-proline+respiration
4374816977L-alanine+respiration
4374816467L-arginine+respiration
4374829991L-aspartate+respiration
4374829985L-glutamate+respiration
4374815971L-histidine+respiration
4374818183L-pyroglutamic acid+respiration
4374817115L-serine+respiration
4374851850methyl pyruvate+respiration
4374817309pectin+respiration
4374817272propionate+respiration
4374817814salicin+respiration

metabolite production

  • @ref: 43748
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
43748catalase+1.11.1.6
43748cytochrome oxidase+1.9.3.1
43748alkaline phosphatase+3.1.3.1
43748esterase (C 4)+
43748esterase Lipase (C 8)+
43748acid phosphatase+3.1.3.2
43748naphthol-AS-BI-phosphohydrolase+
43748lipase (C 14)-
43748leucine arylamidase-3.4.11.1
43748valine arylamidase-
43748cystine arylamidase-3.4.11.3
43748trypsin-3.4.21.4
43748alpha-chymotrypsin-3.4.21.1
43748alpha-galactosidase-3.2.1.22
43748beta-galactosidase-3.2.1.23
43748beta-glucuronidase-3.2.1.31
43748alpha-glucosidase-3.2.1.20
43748beta-glucosidase-3.2.1.21
43748alpha-mannosidase-3.2.1.24
43748alpha-fucosidase-3.2.1.51
43748arginine dihydrolase-3.5.3.6
43748urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43748C16:015.2
    43748C16:1ω7c / C16:1ω6c5.3
    43748C18:1ω7c57
  • type of FA analysis: whole cell analysis
  • incubation medium: TSB
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 2.5
  • incubation_oxygen: aerobic
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: 6850, Agilent
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 43748
  • sample type: Populus euphratica sample at the ancient Khiyik River
  • geographic location: Xinjiang Uyghur Autonomous Region
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 40.722
  • longitude: 85.321

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#River (Creek)
#Host#Plants#Tree

taxonmaps

  • @ref: 69479
  • File name: preview.99_16380.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_1057;97_10061;98_12360;99_16380&stattab=map
  • Last taxonomy: Pseudomonas populi subclade
  • 16S sequence: KC609749
  • Sequence Identity:
  • Total samples: 18063
  • soil counts: 2532
  • aquatic counts: 9095
  • animal counts: 5000
  • plant counts: 1436

Sequence information

16S sequences

  • @ref: 43748
  • description: Partial 16S rRNA gene sequence
  • accession: KC609749
  • database: nuccore

GC content

  • @ref: 43748
  • GC-content: 61.6
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43748

culture collection no.: JCM 19138, CCTCC AB 2013069, NRRL B-59988

literature

  • topic: Phylogeny
  • Pubmed-ID: 26827750
  • title: Pseudomonas populi sp. nov., an endophytic bacterium isolated from Populus euphratica.
  • authors: Anwar N, Abaydulla G, Zayadan B, Abdurahman M, Hamood B, Erkin R, Ismayil N, Rozahon M, Mamtimin H, Rahman E
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000896
  • year: 2016

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43748Nusratgul Anwar, Gulsimay Abaydulla, Bolatkhan Zayadan, Mehfuzem Abdurahman, Buayshem Hamood, Raziya Erkin, Nurimangul Ismayil, Manziram Rozahon, Hormathan Mamtimin, Erkin RahmanPseudomonas populi sp. nov., an endophytic bacterium isolated from Populus euphratica10.1099/ijsem.0.000896IJSEM 66: 1419-1425 201626827750
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/