Strain identifier

BacDive ID: 1409

Type strain: Yes

Species: Falsibacillus pallidus

Strain Designation: CW 7

Strain history: CIP <- 2009, KCTC

NCBI tax ID(s): 493781 (species)

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General

@ref: 17978

BacDive-ID: 1409

DSM-Number: 25281

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Falsibacillus pallidus CW 7 is an aerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from forest soil.

NCBI tax id

  • NCBI tax id: 493781
  • Matching level: species

strain history

@refhistory
376922009, KCTC
17978<- KCTC; KCTC 13200 <- Y. Zhou, Nanjing Agric. Univ., China; CW 7
67771<- Y Zhou, Nanjing Agri. Univ., China
121514CIP <- 2009, KCTC

doi: 10.13145/bacdive1409.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Falsibacillus
  • species: Falsibacillus pallidus
  • full scientific name: Falsibacillus pallidus (Zhou et al. 2008) Zhou et al. 2009

  • synonyms

    • @ref: 20215
    • synonym: Bacillus pallidus

@ref: 17978

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Falsibacillus

species: Falsibacillus pallidus

full scientific name: Falsibacillus pallidus (Zhou et al. 2008) Zhou et al. 2009

strain designation: CW 7

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
23035positive1.8-3.5 µm0.7-1.0 µmrod-shapedyesperitrichous
31181positive1.8-3.5 µm0.7-1 µmrod-shapedyes
67771rod-shapedyesperitrichous
67771positive
69480yes96.21
69480positive100
121514positiverod-shapedyes

colony morphology

  • @ref: 23035
  • colony color: light pink
  • colony shape: circular
  • incubation period: 2 days
  • medium used: TYB medium

pigmentation

  • @ref: 31181
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17978TYB MEDIUM (DSMZ Medium 1247)yeshttps://mediadive.dsmz.de/medium/1247Name: TYB (DSMZ Medium 1247) Composition: Agar 15.0 g/l Tryptone 6.0 g/l Yeast extract 3.0 g/l NaCl 3.0 g/l Beef extract 2.0 g/l FeCl3 0.1 g/l Distilled water
23035TYB mediumyes
37692MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121514CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
121514CIP Medium 566yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566

culture temp

@refgrowthtypetemperaturerange
17978positivegrowth30mesophilic
23035positivegrowth15.0-42.0
23035positiveoptimum30.0-37.0mesophilic
23035mesophilic
31181positivegrowth15-42
31181positiveoptimum30-37mesophilic
37692positivegrowth37mesophilic
67771positivegrowth30-37mesophilic

culture pH

@refabilitytypepHPH range
23035positivegrowth6.0-8.5alkaliphile
23035positiveoptimum7.0-8.0
31181positivegrowth6-8.5
31181positiveoptimum07-08

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23035aerobe
31181aerobe
67771aerobe
121514obligate aerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
23035ellipsoidal,subterminalsporeyes
31181yes
67771sporeyes
69481yes100
69480yes100

halophily

@refsaltgrowthtested relationconcentration
23035NaClnogrowth3.0 %(w/v)
23035NaClnogrowth5.0 %(w/v)
23035NaClnogrowth8.0 %(w/v)

murein

  • @ref: 23035
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

@refobservation
31181aggregates in chains
67771quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
23035174265-dehydro-D-gluconate-builds acid from
2303527613amygdalin-builds acid from
2303518305arbutin-builds acid from
2303517057cellobiose-builds acid from
2303515963ribitol-builds acid from
2303517108D-arabinose-builds acid from
2303518333D-arabitol-builds acid from
2303515824D-fructose-builds acid from
2303528847D-fucose-builds acid from
2303512936D-galactose-builds acid from
2303517634D-glucose-builds acid from
2303517716lactose-builds acid from
2303562318D-lyxose-builds acid from
2303516024D-mannose-builds acid from
2303516988D-ribose-builds acid from
2303516443D-tagatose-builds acid from
2303516813galactitol-builds acid from
2303517113erythritol-builds acid from
2303528066gentiobiose-builds acid from
2303517754glycerol-builds acid from
2303528087glycogen-builds acid from
2303517268myo-inositol-builds acid from
2303515443inulin-builds acid from
2303530849L-arabinose-builds acid from
2303518403L-arabitol-builds acid from
2303518287L-fucose-builds acid from
2303562345L-rhamnose-builds acid from
2303517266L-sorbose-builds acid from
2303565328L-xylose-builds acid from
2303517306maltose-builds acid from
2303529864mannitol-builds acid from
230356731melezitose-builds acid from
2303528053melibiose-builds acid from
23035320061methyl alpha-D-glucopyranoside-builds acid from
2303543943methyl alpha-D-mannoside-builds acid from
2303574863methyl beta-D-xylopyranoside-builds acid from
23035506227N-acetylglucosamine-builds acid from
2303516634raffinose-builds acid from
2303517814salicin-builds acid from
2303530911sorbitol-builds acid from
2303528017starch-builds acid from
2303517992sucrose-builds acid from
2303527082trehalose-builds acid from
2303532528turanose-builds acid from
2303517151xylitol-builds acid from
2303517029chitin-hydrolysis
2303516991dna-hydrolysis
2303517309pectin-hydrolysis
2303528017starch-hydrolysis
2303553426tween 80-hydrolysis
2303518186tyrosine-hydrolysis
2303517632nitrate-reduction
2303516301nitrite-reduction
23035168082-dehydro-D-gluconate+builds acid from
2303565327D-xylose+builds acid from
230354853esculin+builds acid from
2303524265gluconate+builds acid from
23035casein+hydrolysis
230354853esculin+hydrolysis
230355291gelatin+hydrolysis
31181168082-dehydro-D-gluconate+carbon source
3118124265gluconate+carbon source
3118118222xylose+carbon source
12151417632nitrate-reduction
12151416301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
2303535581indoleno
2303516136hydrogen sulfideno
2303515688acetoinyes
12151435581indoleno

metabolite tests

  • @ref: 23035
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
23035arginine dihydrolase-3.5.3.6
23035beta-galactosidase-3.2.1.23
23035catalase+1.11.1.6
23035cytochrome oxidase-1.9.3.1
23035lipase-
23035lysine decarboxylase-4.1.1.18
23035ornithine decarboxylase-4.1.1.17
23035urease-3.5.1.5
31181catalase+1.11.1.6
31181gelatinase+
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
121514oxidase-
121514catalase+1.11.1.6
121514urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121514--------++-----+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
17978forest soilAnhui provinceChinaCHNAsia
67771From forest soilAnhui ProvinceChinaCHNAsia
121514Environment, Soil, forestAnhuiChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
179781Risk group (German classification)
1215141Risk group (French classification)

Sequence information

16S sequences

  • @ref: 17978
  • description: Bacillus [pallidus] strain CW 7 16S ribosomal RNA gene, partial sequence
  • accession: EU364818
  • length: 1413
  • database: ena
  • NCBI tax ID: 493781

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Falsibacillus pallidus DSM 25281GCA_003350505scaffoldncbi493781
66792Falsibacillus pallidus strain DSM 25281493781.3wgspatric493781
66792Falsibacillus pallidus DSM 252812770939515draftimg493781

GC content

@refGC-contentmethod
2303542.3high performance liquid chromatography (HPLC)
6777142.3

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileyes90.019no
flagellatedyes71.612no
gram-positiveyes89.496yes
anaerobicno97.928yes
aerobicyes87.963no
halophileyes50.814no
spore-formingyes96.283no
glucose-utilyes89.388no
thermophileno98.53no
glucose-fermentno94.945no

External links

@ref: 17978

culture collection no.: DSM 25281, CCTCC AB 207188, KCTC 13200, LMG 24451, CIP 109936, BCRC 80032

straininfo link

  • @ref: 71060
  • straininfo: 323287

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19060070Bacillus pallidus sp. nov., isolated from forest soil.Zhou Y, Wei W, Che Q, Xu Y, Wang X, Huang X, Lai RInt J Syst Evol Microbiol10.1099/ijs.0.2008/000075-02008Bacillus/chemistry/*classification/genetics/*physiology/ultrastructure, Fatty Acids/analysis, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology, Species Specificity, Trees/*microbiologyGenetics
Phylogeny19767356Falsibacillus pallidus to replace the homonym Bacillus pallidus Zhou et al. 2008.Zhou Y, Xu J, Xu L, Tindall BJInt J Syst Evol Microbiol10.1099/ijs.0.014316-02009Bacillus/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, *Terminology as TopicGenetics
Phylogeny30839250Falsibacillus albus sp. nov., isolated from mangrove soil.Shi SB, Liu C, Jiang MG, Li GD, Yang LF, Wu JF, Jiang LQ, Zhang K, Shen NK, Jiang CL, Jiang YInt J Syst Evol Microbiol10.1099/ijsem.0.0033282019Bacillaceae/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Glycolipids/chemistry, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Rhizophoraceae/*microbiology, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
17978Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25281)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25281
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23035Yu Zhou,Junfeng Xu,Lihong Xu,Brian J. Tindall10.1099/ijs.0.014316-0Falsibacillus pallidus to replace the homonym Bacillus pallidus Zhou et al. 2008IJSEM 59: 3176-3180 200919767356
31181Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604127508
37692Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7736
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71060Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID323287.1StrainInfo: A central database for resolving microbial strain identifiers
121514Curators of the CIPCollection of Institut Pasteur (CIP 109936)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109936