Strain identifier

BacDive ID: 140809

Type strain: Yes

Species: Bradyrhizobium stylosanthis

Strain Designation: H-8

NCBI tax ID(s): 1803665 (species)

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General

@ref: 43595

BacDive-ID: 140809

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Bradyrhizobium stylosanthis H-8 is a mesophilic, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from an effective nodule of Stylosanthes guianensis, a perennial tropical forage legume.

NCBI tax id

  • NCBI tax id: 1803665
  • Matching level: species

doi: 10.13145/bacdive140809.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Nitrobacteraceae
  • genus: Bradyrhizobium
  • species: Bradyrhizobium stylosanthis
  • full scientific name: Bradyrhizobium stylosanthis Delamuta et al. 2016

@ref: 43595

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Hyphomicrobiales

family: Bradyrhizobiaceae

genus: Bradyrhizobium

species: Bradyrhizobium stylosanthis

strain designation: H-8

type strain: yes

Morphology

cell morphology

  • @ref: 43595
  • gram stain: negative
  • cell shape: rod-shaped

colony morphology

  • @ref: 43595
  • colony size: 1-1.34 mm
  • colony color: slightly pink
  • colony shape: circular
  • incubation period: 7 days
  • medium used: YMA medium

Culture and growth conditions

culture medium

  • @ref: 43595
  • name: YMA medium
  • growth: yes
  • composition: with Congo Red

culture temp

@refgrowthtypetemperaturerange
43595nogrowth37mesophilic
43595positiveoptimum28mesophilic

culture pH

@refabilitytypepH
43595positivegrowth4.5-8
43595positiveoptimum6.8

Physiology and metabolism

spore formation

  • @ref: 43595
  • spore formation: no

halophily

  • @ref: 43595
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 1 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4359517924D-sorbitol-carbon source
4359527613amygdalin-fermentation
4359518305arbutin-fermentation
4359517057cellobiose-fermentation
4359515963ribitol-fermentation
4359517924D-sorbitol-fermentation
4359516443D-tagatose-fermentation
4359516813galactitol-fermentation
4359517113erythritol-fermentation
4359528066gentiobiose-fermentation
4359517268myo-inositol-fermentation
4359515443inulin-fermentation
4359518403L-arabitol-fermentation
4359517266L-sorbose-fermentation
4359517716lactose-fermentation
4359517306maltose-fermentation
435956731melezitose-fermentation
4359528053melibiose-fermentation
43595320061methyl alpha-D-glucopyranoside-fermentation
4359543943methyl alpha-D-mannoside-fermentation
43595506227N-acetylglucosamine-fermentation
43595potassium 2-dehydro-D-gluconate-fermentation
4359516634raffinose-fermentation
4359517814salicin-fermentation
4359517992sucrose-fermentation
4359527082trehalose-fermentation
4359532528turanose-fermentation
4359517151xylitol-fermentation
43595esculin ferric citrate+carbon source
4359528087glycogen+carbon source
4359565328L-xylose+carbon source
43595potassium 5-dehydro-D-gluconate+carbon source
4359532032potassium gluconate+carbon source
4359517108D-arabinose+fermentation
4359562318D-lyxose+fermentation
43595esculin ferric citrate+fermentation
4359528087glycogen+fermentation
4359530849L-arabinose+fermentation
4359518287L-fucose+fermentation
4359565328L-xylose+fermentation
43595potassium 5-dehydro-D-gluconate+fermentation
4359532032potassium gluconate+fermentation
4359528017starch+fermentation
4359517108D-arabinose+/-carbon source
4359528847D-fucose+/-carbon source
4359516988D-ribose+/-carbon source
4359530849L-arabinose+/-carbon source
4359574863methyl beta-D-xylopyranoside+/-carbon source
4359518333D-arabitol+/-fermentation
4359515824D-fructose+/-fermentation
4359528847D-fucose+/-fermentation
4359512936D-galactose+/-fermentation
4359517634D-glucose+/-fermentation
4359516899D-mannitol+/-fermentation
4359516024D-mannose+/-fermentation
4359516988D-ribose+/-fermentation
4359565327D-xylose+/-fermentation
4359517754glycerol+/-fermentation
4359562345L-rhamnose+/-fermentation
4359574863methyl beta-D-xylopyranoside+/-fermentation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
4359528971ampicillinyesyes10 µg (disc)
435957507neomycinyesyes30 µg (disc)
4359527902tetracyclineyesyes30 µg (disc)
43595100147nalidixic acidyesyes30 µg (disc)
435953515cefuroximeyesyes
4359517076streptomycinyesyes
4359548923erythromycinyesyes15 µg (disc)
4359518208penicillin gyesyes
4359517698chloramphenicolyesyes
4359528669bacitracinyesyes

enzymes

  • @ref: 43595
  • value: urease
  • activity: +
  • ec: 3.5.1.5

Isolation, sampling and environmental information

isolation

  • @ref: 43595
  • sample type: an effective nodule of Stylosanthes guianensis, a perennial tropical forage legume
  • geographic location: Brazil
  • country: Brazil
  • origin.country: BRA
  • continent: Middle and South America

isolation source categories

Cat1Cat2Cat3
#Climate#Hot#Tropical
#Host#Plants#Shrub (Scrub)
#Host Body-Site#Plant#Root nodule

taxonmaps

  • @ref: 69479
  • File name: preview.99_871.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_86;96_297;97_329;98_370;99_871&stattab=map
  • Last taxonomy: Bradyrhizobium
  • 16S sequence: KU724142
  • Sequence Identity:
  • Total samples: 5154
  • soil counts: 1424
  • aquatic counts: 688
  • animal counts: 2839
  • plant counts: 203

Sequence information

16S sequences

  • @ref: 43595
  • description: 16S rRNA gene sequence
  • accession: KU724142
  • database: nuccore

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bradyrhizobium stylosanthis BR 446GCA_001641335contigncbi1803665
66792Bradyrhizobium sp. BR 4461803665.3wgspatric1803665
66792Bradyrhizobium stylosanthis BR 4462791355195draftimg1803665

GC content

  • @ref: 43595
  • GC-content: 63.9
  • method: real time PCR (RTD-PCR)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes88.092no
gram-positiveno98.34yes
anaerobicno98.074no
aerobicyes94.943no
halophileno96.068no
spore-formingno92.963yes
glucose-utilyes89.375no
flagellatedno53.934no
thermophileno99.491no
glucose-fermentno91.53no

External links

@ref: 43595

culture collection no.: CNPSo 2823, HAMBI 3668, BR 446

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27169861Bradyrhizobium stylosanthis sp. nov., comprising nitrogen-fixing symbionts isolated from nodules of the tropical forage legume Stylosanthes spp.Delamuta JRM, Ribeiro RA, Araujo JLS, Rouws LFM, Zilli JE, Parma MM, Melo IS, Hungria MInt J Syst Evol Microbiol10.1099/ijsem.0.0011482016Bacterial Typing Techniques, Base Composition, Bradyrhizobium/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, DNA, Ribosomal Spacer/genetics, Fabaceae/*microbiology, Fatty Acids/chemistry, Genes, Bacterial, Multilocus Sequence Typing, Nitrogen Fixation, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Root Nodules, Plant/*microbiology, Sequence Analysis, DNATranscriptome
Genetics27365354Genome Sequence of Bradyrhizobium stylosanthis Strain BR 446T, a Nitrogen-Fixing Symbiont of the Legume Pasture Stylosanthes guianensis.Delamuta JR, Ribeiro RA, Gomes DF, Souza RC, Chueire LM, Hungria MGenome Announc10.1128/genomeA.00631-162016

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43595Jakeline Renata Marçon Delamuta, Renan Augusto Ribeiro, Jean Luiz Simões Araújo, Luc Felicianus Marie Rouws, Jerri Édson Zilli, Marcia Maria Parma, Itamar Soares Melo, Mariangela HungriaBradyrhizobium stylosanthis sp. nov., comprising nitrogen-fixing symbionts isolated from nodules of the tropical forage legume Stylosanthes spp.10.1099/ijsem.0.001148IJSEM 66: 3078-3087 201627169861
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/