Name: Bradyrhizobium stylosanthis H-8
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140809

Type strain:

Species: Bradyrhizobium stylosanthis

Strain Designation: H-8

Culture col. no.: CNPSo 2823, HAMBI 3668, BR 446

NCBI tax ID(s): 1803665 (species)

Section

Bradyrhizobium stylosanthis H-8 is a mesophilic, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from an effective nodule of Stylosanthes guianensis, a perennial tropical forage legume.

colony-forming
Gram-negative
rod-shaped
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Nitrobacteraceae*
	Genus	*Bradyrhizobium*
	Species	**Bradyrhizobium stylosanthis**
	Full Scientific Name (LPSN)	Bradyrhizobium stylosanthis Delamuta et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43595]	Gram stain	negative

[Ref.: #43595]	Cell shape	rod-shaped

[Ref.: #43595]	Colony size	1-1.34 mm
[Ref.: #43595]	Colony color	slightly pink
[Ref.: #43595]	Colony shape	circular
[Ref.: #43595]	Incubation period	7 days
[Ref.: #43595]	Cultivation medium used	YMA medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43595]

Culture medium

YMA medium

[Ref.: #43595]

Culture medium growth

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Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #43595]		no	growth	37 °C
[Ref.: #43595]			optimum	28 °C

[Ref.: #43595]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #43595]			optimum	6.8
[Ref.: #43595]			growth	4.5-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43595]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43595]		NaCl	no	growth	1	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43595]	amygdalin ChEBI	-	fermentation
[Ref.: #43595]	arbutin ChEBI	-	fermentation
[Ref.: #43595]	cellobiose ChEBI	-	fermentation
[Ref.: #43595]	D-arabinose ChEBI	+	fermentation
[Ref.: #43595]	D-arabinose ChEBI	+/-	carbon source
[Ref.: #43595]	D-arabitol ChEBI	+/-	fermentation
[Ref.: #43595]	D-fructose ChEBI	+/-	fermentation
[Ref.: #43595]	D-fucose ChEBI	+/-	carbon source
[Ref.: #43595]	D-fucose ChEBI	+/-	fermentation
[Ref.: #43595]	D-galactose ChEBI	+/-	fermentation
[Ref.: #43595]	D-glucose ChEBI	+/-	fermentation
[Ref.: #43595]	D-lyxose ChEBI	+	fermentation
[Ref.: #43595]	D-mannitol ChEBI	+/-	fermentation
[Ref.: #43595]	D-mannose ChEBI	+/-	fermentation
[Ref.: #43595]	D-ribose ChEBI	+/-	carbon source
[Ref.: #43595]	D-ribose ChEBI	+/-	fermentation
[Ref.: #43595]	D-sorbitol ChEBI	-	carbon source
[Ref.: #43595]	D-sorbitol ChEBI	-	fermentation
[Ref.: #43595]	D-tagatose ChEBI	-	fermentation
[Ref.: #43595]	D-xylose ChEBI	+/-	fermentation
[Ref.: #43595]	erythritol ChEBI	-	fermentation
[Ref.: #43595]	esculin ferric citrate	+	carbon source
[Ref.: #43595]	esculin ferric citrate	+	fermentation
[Ref.: #43595]	galactitol ChEBI	-	fermentation
[Ref.: #43595]	gentiobiose ChEBI	-	fermentation
[Ref.: #43595]	glycerol ChEBI	+/-	fermentation
[Ref.: #43595]	glycogen ChEBI	+	carbon source
[Ref.: #43595]	glycogen ChEBI	+	fermentation
[Ref.: #43595]	inulin ChEBI	-	fermentation
[Ref.: #43595]	L-arabinose ChEBI	+	fermentation
[Ref.: #43595]	L-arabinose ChEBI	+/-	carbon source
[Ref.: #43595]	L-arabitol ChEBI	-	fermentation
[Ref.: #43595]	L-fucose ChEBI	+	fermentation
[Ref.: #43595]	L-rhamnose ChEBI	+/-	fermentation
[Ref.: #43595]	L-sorbose ChEBI	-	fermentation
[Ref.: #43595]	L-xylose ChEBI	+	carbon source
[Ref.: #43595]	L-xylose ChEBI	+	fermentation
[Ref.: #43595]	lactose ChEBI	-	fermentation
[Ref.: #43595]	maltose ChEBI	-	fermentation
[Ref.: #43595]	melezitose ChEBI	-	fermentation
[Ref.: #43595]	melibiose ChEBI	-	fermentation
[Ref.: #43595]	methyl alpha-D-glucopyranoside ChEBI	-	fermentation
[Ref.: #43595]	methyl alpha-D-mannoside ChEBI	-	fermentation
[Ref.: #43595]	methyl beta-D-xylopyranoside ChEBI	+/-	carbon source
[Ref.: #43595]	methyl beta-D-xylopyranoside ChEBI	+/-	fermentation
[Ref.: #43595]	myo-inositol ChEBI	-	fermentation
[Ref.: #43595]	N-acetylglucosamine ChEBI	-	fermentation
[Ref.: #43595]	potassium 2-dehydro-D-gluconate	-	fermentation
[Ref.: #43595]	potassium 5-dehydro-D-gluconate	+	carbon source
[Ref.: #43595]	potassium 5-dehydro-D-gluconate	+	fermentation
[Ref.: #43595]	potassium gluconate ChEBI	+	carbon source
[Ref.: #43595]	potassium gluconate ChEBI	+	fermentation
[Ref.: #43595]	raffinose ChEBI	-	fermentation
[Ref.: #43595]	ribitol ChEBI	-	fermentation
[Ref.: #43595]	salicin ChEBI	-	fermentation
[Ref.: #43595]	starch ChEBI	+	fermentation
[Ref.: #43595]	sucrose ChEBI	-	fermentation
[Ref.: #43595]	trehalose ChEBI	-	fermentation
[Ref.: #43595]	turanose ChEBI	-	fermentation
[Ref.: #43595]	xylitol ChEBI	-	fermentation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43595]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #43595]	bacitracin ChEBI				yes
[Ref.: #43595]	cefuroxime ChEBI	yes
[Ref.: #43595]	chloramphenicol ChEBI				yes
[Ref.: #43595]	erythromycin ChEBI				yes	15	µg (disc)
[Ref.: #43595]	nalidixic acid ChEBI	yes	30	µg (disc)
[Ref.: #43595]	neomycin ChEBI	yes	30	µg (disc)
[Ref.: #43595]	penicillin g ChEBI				yes
[Ref.: #43595]	streptomycin ChEBI	yes
[Ref.: #43595]	tetracycline ChEBI	yes	30	µg (disc)

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43595]		urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43595]	Sample type/isolated from	an effective nodule of Stylosanthes guianensis, a perennial tropical forage legume
[Ref.: #43595]	Geographic location (country and/or sea, region)	Brazil
[Ref.: #43595]	Country	Brazil
[Ref.: #43595]	Country ISO 3 Code	BRA
[Ref.: #43595]	Continent	Middle and South America
* marker position based on {}

Isolation sources categories

#Climate	#Hot	#Tropical
#Host	#Plants	#Shrub (Scrub)
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

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Global distribution of 16S sequence KU724142 (>99% sequence identity) for Bradyrhizobium from Microbeatlas

Aquatic Samples	688
Soil Samples	1424
Animal Samples	2839
Plant Samples	203
Total Samples	5154

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43595]	16S rRNA gene sequence	KU724142		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bradyrhizobium stylosanthis BR 446	GCA_001641335	contig	GenBank	1803665 tax ID	*
[Ref.: #66792]	Bradyrhizobium sp. BR 446	1803665.3	wgs	PATRIC	1803665 tax ID	*
[Ref.: #66792]	Bradyrhizobium stylosanthis BR 446	2791355195	draft	JGI IMG/M	1803665 tax ID	*

[Ref.: #43595]

GC-content

63.9 mol%

real time PCR (RTD-PCR)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bradyrhizobium sp. BR 446 PATRIC: 1803665.3
[Ref.: #69480]	motile	yes	88.092	no
[Ref.: #69480]	gram-positive	no	98.34	yes
[Ref.: #69480]	anaerobic	no	98.074	no
[Ref.: #69480]	aerobic	yes	94.943	no
[Ref.: #69480]	halophile	no	96.068	no
[Ref.: #69480]	spore-forming	no	92.963	yes
[Ref.: #69480]	glucose-util	yes	89.375	no
[Ref.: #69480]	flagellated	no	53.934	no
[Ref.: #69480]	thermophile	no	99.491	no
[Ref.: #69480]	glucose-ferment	no	91.53	no

Availability in culture collections External links

[Ref.: #43595]

Culture collection no.

CNPSo 2823, HAMBI 3668, BR 446

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome Sequence of Bradyrhizobium stylosanthis Strain BR 446T, a Nitrogen-Fixing Symbiont of the Legume Pasture Stylosanthes guianensis.	Delamuta JR, Ribeiro RA, Gomes DF, Souza RC, Chueire LM, Hungria M	Genome Announc	10.1128/genomeA.00631-16	2016	*
Phylogeny	Bradyrhizobium stylosanthis sp. nov., comprising nitrogen-fixing symbionts isolated from nodules of the tropical forage legume Stylosanthes spp.	Delamuta JRM, Ribeiro RA, Araujo JLS, Rouws LFM, Zilli JE, Parma MM, Melo IS, Hungria M	Int J Syst Evol Microbiol	10.1099/ijsem.0.001148	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43595

Jakeline Renata Marçon Delamuta, Renan Augusto Ribeiro, Jean Luiz Simões Araújo, Luc Felicianus Marie Rouws, Jerri Édson Zilli, Marcia Maria Parma, Itamar Soares Melo, Mariangela Hungria: Bradyrhizobium stylosanthis sp. nov., comprising nitrogen-fixing symbionts isolated from nodules of the tropical forage legume Stylosanthes spp.. IJSEM 66: 3078 - 3087 2016 ( DOI 10.1099/ijsem.0.001148 , PubMed 27169861 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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Bradyrhizobium sp. BR 446

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