Strain identifier

BacDive ID: 140683

Type strain: Yes

Species: Mucilaginibacter craterilacus

Strain Designation: N60A

Strain history: <- Jung-Sook Lee, KRIBB

NCBI tax ID(s): 1861875 (species)

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General

@ref: 43448

BacDive-ID: 140683

keywords: 16S sequence, Bacteria, obligate aerobe, Gram-negative, rod-shaped, colony-forming

description: Mucilaginibacter craterilacus N60A is an obligate aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from sediment soil of crater lake, Baekrokdam, Hallasan, Jeju.

NCBI tax id

  • NCBI tax id: 1861875
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Jung-Sook Lee, KRIBB

doi: 10.13145/bacdive140683.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Sphingobacteriia
  • order: Sphingobacteriales
  • family: Sphingobacteriaceae
  • genus: Mucilaginibacter
  • species: Mucilaginibacter craterilacus
  • full scientific name: Mucilaginibacter craterilacus Lee et al. 2017

@ref: 43448

domain: Bacteria

phylum: Bacteroidetes

class: Sphingobacteriia

order: Sphingobacteriales

family: Sphingobacteriaceae

genus: Mucilaginibacter

species: Mucilaginibacter craterilacus

strain designation: N60A

type strain: yes

Morphology

cell morphology

  • @ref: 43448
  • gram stain: negative
  • cell length: 1.8-3.2 µm
  • cell width: 0.6-0.9 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 43448
  • colony color: transparent, white
  • colony shape: circular
  • medium used: 10x diluted R2A

pigmentation

  • @ref: 43448
  • production: yes
  • name: Flexirubin

Culture and growth conditions

culture medium

  • @ref: 43448
  • name: tenfold diluted R2A
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
43448positivegrowth10-30
43448positiveoptimum25mesophilic
67771positivegrowth25mesophilic

culture pH

@refabilitytypepH
43448positivegrowth6-7
43448positiveoptimum6.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43448obligate aerobe
67771aerobe

spore formation

  • @ref: 43448
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentrationhalophily level
43448NaClpositivegrowth0.5 %
43448NaClpositiveoptimum0 %non-halophilic

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4344817128adipate-assimilation
4344822599arabinose-assimilation
4344827689decanoate-assimilation
4344817234glucose-assimilation
4344825115malate-assimilation
4344817306maltose-assimilation
4344829864mannitol-assimilation
4344837684mannose-assimilation
43448506227N-acetylglucosamine-assimilation
4344818401phenylacetate-assimilation
4344832032potassium gluconate-assimilation
4344853258sodium citrate-assimilation
4344827613amygdalin-builds acid from
4344818305arbutin-builds acid from
4344817057cellobiose-builds acid from
4344815824D-fructose-builds acid from
4344812936D-galactose-builds acid from
4344817634D-glucose-builds acid from
4344816024D-mannose-builds acid from
4344828066gentiobiose-builds acid from
4344815443inulin-builds acid from
4344830849L-arabinose-builds acid from
4344817716lactose-builds acid from
4344817306maltose-builds acid from
434486731melezitose-builds acid from
4344828053melibiose-builds acid from
43448506227N-acetylglucosamine-builds acid from
4344817814salicin-builds acid from
4344817992sucrose-builds acid from
4344827082trehalose-builds acid from
4344832528turanose-builds acid from
4344829016arginine-hydrolysis
4344835581indole-reduction
4344817632nitrate-reduction
4344817057cellobiose-respiration
4344814314D-glucose 6-phosphate-respiration
4344852071dextran-respiration
4344829042glucose 1-phosphate-respiration
4344817754glycerol-respiration
4344814336glycerol 1-phosphate-respiration
4344828087glycogen-respiration
4344829985L-glutamate-respiration
4344817203L-proline-respiration
4344817115L-serine-respiration
4344816857L-threonine-respiration
4344837657methyl D-glucoside-respiration
4344851850methyl pyruvate-respiration
43448506227N-acetylglucosamine-respiration
4344873784glycyl-l-glutamate-respiration
4344840585alpha-cyclodextrin+assimilation
4344817925alpha-D-glucose+assimilation
4344815824D-fructose+assimilation
4344816024D-mannose+assimilation
4344828066gentiobiose+assimilation
4344817716lactose+assimilation
4344817306maltose+assimilation
4344817992sucrose+assimilation
4344827082trehalose+assimilation
4344832528turanose+assimilation
43448esculin ferric citrate+builds acid from
4344840585alpha-cyclodextrin+carbon source
4344817925alpha-D-glucose+carbon source
4344815824D-fructose+carbon source
4344812936D-galactose+carbon source
4344816024D-mannose+carbon source
4344828066gentiobiose+carbon source
4344817716lactose+carbon source
434486359lactulose+carbon source
4344817306maltose+carbon source
4344828053melibiose+carbon source
4344816634raffinose+carbon source
4344817992sucrose+carbon source
4344827082trehalose+carbon source
4344832528turanose+carbon source
434484853esculin+hydrolysis
434485291gelatin+hydrolysis
434486359lactulose+respiration
4344828053melibiose+respiration
4344816634raffinose+respiration
434483557154-nitrophenyl beta-D-galactopyranoside+/-hydrolysis

metabolite production

  • @ref: 43448
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
43448catalase+1.11.1.6
43448cytochrome oxidase+1.9.3.1
43448urease-3.5.1.5
43448alkaline phosphatase+3.1.3.1
43448esterase (C 4)+
43448esterase Lipase (C 8)+
43448leucine arylamidase+3.4.11.1
43448valine arylamidase+
43448cystine arylamidase+3.4.11.3
43448acid phosphatase+3.1.3.2
43448naphthol-AS-BI-phosphohydrolase+
43448alpha-galactosidase+3.2.1.22
43448beta-galactosidase+3.2.1.23
43448alpha-glucosidase+3.2.1.20
43448beta-glucosidase+3.2.1.21
43448N-acetyl-beta-glucosaminidase+3.2.1.52
43448alpha-fucosidase+3.2.1.51
43448lipase (C 14)-
43448trypsin-3.4.21.4
43448alpha-chymotrypsin-3.4.21.1
43448beta-glucuronidase-3.2.1.31
43448alpha-mannosidase+/-3.2.1.24

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    43448C15:0 anteiso6.1
    43448C14:01.2
    43448C15:01.6
    43448C16:04.8
    43448C16:1ω5c3.1
    43448C16:1ω7c / C16:1ω6c39.6
    43448C15:0 iso29.1
    43448C15:0 iso 3OH1.3
    43448C16:0 iso1
    43448C17:0 iso 3OH7.9
    43448C17:1 iso ω9c / C16:0 10-methyl2.2
    43448Unknown ECL 13.5651.213565
  • type of FA analysis: whole cell analysis
  • incubation medium: one-tenth-strength R2A
  • agar/liquid: agar
  • incubation temperature: 25
  • incubation time: 5
  • incubation_oxygen: aerobic
  • library/peak naming table: TSBA6.1
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
43448sediment soil of crater lake, Baekrokdam, Hallasan, JejuBaekrokdam, Hallasan, JejuRepublic of KoreaKORAsia
67771From soilJejuRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Sediment
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_87297.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_115;96_22952;97_39931;98_51978;99_87297&stattab=map
  • Last taxonomy: Mucilaginibacter craterilacus subclade
  • 16S sequence: LC160265
  • Sequence Identity:
  • Total samples: 371
  • soil counts: 322
  • aquatic counts: 13
  • animal counts: 12
  • plant counts: 24

Sequence information

16S sequences

  • @ref: 43448
  • description: 16S rRNA gene sequence
  • accession: LC160265
  • database: nuccore

GC content

  • @ref: 43448
  • GC-content: 44.4
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43448

culture collection no.: KCTC 52404, NRRL B-65396

literature

  • topic: Phylogeny
  • Pubmed-ID: 28820098
  • title: Mucilaginibacter craterilacus sp. nov., isolated from sediment soil of a crater lake.
  • authors: Lee KC, Kim KK, Eom MK, Kim JS, Kim DS, Ko SH, Yang SH, Lee JS
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002043
  • year: 2017
  • mesh: Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Lakes/*microbiology, Phosphatidylethanolamines/*chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43448Keun Chul Lee, Kwang Kyu Kim, Mi Kyung Eom, Jong-Shik Kim, Dae-Shin Kim, Suk-Hyung Ko, Seung-Hoon Yang, Jung-Sook LeeMucilaginibacter craterilacus sp. nov., isolated from sediment soil of a crater lake10.1099/ijsem.0.002043IJSEM 67: 2891-2896 201728820098
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/