Strain identifier

BacDive ID: 140682

Type strain: Yes

Species: Flavobacterium flaviflagrans

Strain Designation: CB-3

NCBI tax ID(s): 2027552 (species)

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General

@ref: 43447

BacDive-ID: 140682

keywords: 16S sequence, Bacteria, aerobe, thermophilic, Gram-negative, rod-shaped, colony-forming

description: Flavobacterium flaviflagrans CB-3 is an aerobe, thermophilic, Gram-negative bacterium that forms circular colonies and was isolated from forest soil in Kyonggi University, Suwon, Gyeonggi-Do.

NCBI tax id

  • NCBI tax id: 2027552
  • Matching level: species

doi: 10.13145/bacdive140682.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Flavobacterium
  • species: Flavobacterium flaviflagrans
  • full scientific name: Flavobacterium flaviflagrans Dahal et al. 2017

@ref: 43447

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Flavobacterium

species: Flavobacterium flaviflagrans

strain designation: CB-3

type strain: yes

Morphology

cell morphology

  • @ref: 43447
  • gram stain: negative
  • cell length: 1.9-2.7 µm
  • cell width: 0.4-0.8 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 43447
  • colony size: 2-3 mm
  • colony color: Yellow
  • colony shape: circular
  • incubation period: 4 days
  • medium used: TSA agar

pigmentation

  • @ref: 43447
  • production: no
  • name: Flexirubin

Culture and growth conditions

culture medium

@refnamegrowth
43447BHIyes
43447LBAyes
43447MacConkey agarno
43447Marine agar (MA)no
43447Nutrient agar (NA)yes
43447PDAno
43447Reasoner's 2A agar (R2A)yes
43447Trypticase Soy Agar (TSA)yes
43447Veal infusion agaryes

culture temp

@refgrowthtypetemperaturerange
43447positivemaximum42thermophilic
43447positivegrowth15-42
43447positiveoptimum20-35

culture pH

@refabilitytypepHPH range
43447positivegrowth5.5-9.5alkaliphile
43447positiveoptimum7-9

Physiology and metabolism

oxygen tolerance

  • @ref: 43447
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43447
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentrationhalophily level
43447NaClpositivemaximum1.5 %(w/v)
43447NaClpositiveoptimum0 %(w/v)non-halophilic

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4344727897tryptophan-energy source
4344717234glucose-fermentation
4344785146carboxymethylcellulose-hydrolysis
4344717029chitin-hydrolysis
434474853esculin-hydrolysis
4344728017starch-hydrolysis
4344753425tween 60-hydrolysis
4344753426tween 80-hydrolysis
4344717632nitrate-reduction
43447casein+hydrolysis
4344716991dna+hydrolysis
434475291gelatin+hydrolysis
43447esculin ferric citrate+/-hydrolysis
4344753423tween 40+/-hydrolysis
4344718186tyrosine+/-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
4344716136hydrogen sulfideno
4344735581indoleno

metabolite tests

  • @ref: 43447
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
43447cytochrome oxidase+1.9.3.1
43447urease-3.5.1.5
43447catalase-1.11.1.6
43447DNase+
43447alkaline phosphatase+3.1.3.1
43447esterase (C 4)+
43447esterase Lipase (C 8)+
43447leucine arylamidase+3.4.11.1
43447valine arylamidase+
43447trypsin+3.4.21.4
43447alpha-chymotrypsin+3.4.21.1
43447acid phosphatase+3.1.3.2
43447naphthol-AS-BI-phosphohydrolase+
43447N-acetyl-beta-glucosaminidase+3.2.1.52
43447cystine arylamidase+/-3.4.11.3
43447lipase (C 14)-
43447alpha-galactosidase-3.2.1.22
43447beta-galactosidase-3.2.1.23
43447beta-glucuronidase-3.2.1.31
43447alpha-glucosidase-3.2.1.20
43447beta-glucosidase-3.2.1.21
43447alpha-mannosidase-3.2.1.24
43447alpha-fucosidase-3.2.1.51
43447tryptophan deaminase-4.1.99.1

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43447C15:0 anteiso0.7
    43447C13:00.2
    43447C14:00.3
    43447C14:1ω5c0.1
    43447C15:0 2OH0.2
    43447C15:0 3OH1.3
    43447C15:1ω6c0.7
    43447C16:00.2
    43447C16:0 3OH0.6
    43447C16:1ω7c / C16:1ω6c2
    43447C17:0 2OH0.1
    43447C18:00.1
    43447C18:1ω5c1.1
    43447C8:0 3OH0.1
    43447C11:0 iso 3OH0.2
    43447C13:0 iso2.2
    43447C13:0 iso 3OH0.2
    43447C14:0 iso0.3
    43447C14:0 iso 3OH0.2
    43447C15:0 iso51.8
    43447C15:0 iso 3OH12
    43447C15:1 iso G4.1
    43447C16:0 iso0.2
    43447C16:0 iso 3OH0.3
    43447C17:0 iso0.1
    43447C17:0 iso 3OH10.9
    43447C17:1 iso ω9c / C16:0 10-methyl9.4
    43447Unknown 10.928 / C12:0 aldehyde0.3
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 3
  • incubation_oxygen: aerobic
  • software version: Sherlock 6.0B
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 43447
  • sample type: forest soil in Kyonggi University, Suwon, Gyeonggi-Do
  • geographic location: Gyeonggi-Do, Suwon
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_62934.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_66;96_1250;97_1483;98_1808;99_62934&stattab=map
  • Last taxonomy: Flavobacterium
  • 16S sequence: KY031328
  • Sequence Identity:
  • Total samples: 4267
  • soil counts: 1132
  • aquatic counts: 2409
  • animal counts: 603
  • plant counts: 123

Sequence information

16S sequences

  • @ref: 43447
  • description: 16S rRNA gene sequence
  • accession: KY031328
  • database: nuccore

GC content

  • @ref: 43447
  • GC-content: 35.8
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43447

culture collection no.: KEMB 9005-535, KACC 19112, NBRC 112704

literature

  • topic: Phylogeny
  • Pubmed-ID: 28809147
  • title: Flavobacterium flaviflagrans sp. nov., a bacterium of the family Flavobacteriaceae isolated from forest soil.
  • authors: Dahal RH, Chaudhary DK, Kim J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.002000
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, *Forests, Phosphatidylethanolamines/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43447Ram Hari Dahal, Dhiraj Kumar Chaudhary, Jaisoo KimFlavobacterium flaviflagrans sp. nov., a bacterium of the family Flavobacteriaceae isolated from forest soil10.1099/ijsem.0.002000IJSEM 67: 2653-2659 201728809147
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/