Strain identifier

BacDive ID: 140577

Type strain: Yes

Species: Dyella lipolytica

Strain Designation: DHOB07

Strain history: <- Lihong QIU, Sun Yat-Sen Univ., China

NCBI tax ID(s): 1867835 (species)

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General

@ref: 43180

BacDive-ID: 140577

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped, colony-forming

description: Dyella lipolytica DHOB07 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from forest soil of Dinghushan Biosphere Reserve.

NCBI tax id

  • NCBI tax id: 1867835
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Lihong QIU, Sun Yat-Sen Univ., China

doi: 10.13145/bacdive140577.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Lysobacterales
  • family: Rhodanobacteraceae
  • genus: Dyella
  • species: Dyella lipolytica
  • full scientific name: Dyella lipolytica Tang et al. 2017

@ref: 43180

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Lysobacterales

family: Rhodanobacteraceae

genus: Dyella

species: Dyella lipolytica

strain designation: DHOB07

type strain: yes

Morphology

cell morphology

  • @ref: 43180
  • gram stain: negative
  • cell length: 1.0-2.5 µm
  • cell width: 0.3-0.6 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 43180
  • colony size: 1.2-1.7 mm
  • colony color: yellow
  • colony shape: circular
  • incubation period: 2 days
  • medium used: R2A agar

Culture and growth conditions

culture medium

@refnamegrowth
43180Reasoner's 2A agar (R2A)yes
43180Nutrient agar (NA)no
43180Trypticase Soy Agar (TSA)no
43180LB (Luria-Bertani) MEDIUMno
43180MacConkey agarno

culture temp

@refgrowthtypetemperaturerange
43180positiveoptimum28mesophilic
43180positivegrowth10-37
67771positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
43180positivegrowth4-7acidophile
43180positiveoptimum5-5.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43180aerobe
67771aerobe

spore formation

  • @ref: 43180
  • type of spore: spore
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
43180NaClpositivegrowth0-0.5 %(w/v)
43180NaClpositiveoptimum0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4318017632nitrate-reduction
4318053426tween 80+hydrolysis
43180casein-hydrolysis
431805291gelatin-hydrolysis
431804853esculin-hydrolysis
4318028017starch-hydrolysis
4318017128adipate+carbon source
43180esculin ferric citrate+carbon source
4318018305arbutin+carbon source
4318017108D-arabinose+carbon source
4318017634D-glucose+carbon source
431806731melezitose+carbon source
4318016634raffinose+carbon source
4318017924D-sorbitol+carbon source
4318016443D-tagatose+carbon source
4318027082trehalose+carbon source
4318017113erythritol+carbon source
4318028087glycogen+carbon source
4318017268myo-inositol+carbon source
4318015443inulin+carbon source
4318018287L-fucose+carbon source
4318025115malate+carbon source
43180506227N-acetylglucosamine+carbon source
4318017234glucose-fermentation
4318027613amygdalin-carbon source
4318027689decanoate-carbon source
4318017057cellobiose-carbon source
4318012936D-galactose+carbon source
4318028066gentiobiose-carbon source
4318017716lactose-carbon source
4318062318D-lyxose-carbon source
4318028053melibiose-carbon source
4318017992sucrose-carbon source
4318032528turanose-carbon source
4318065327D-xylose-carbon source
4318030849L-arabinose-carbon source
43180320061methyl alpha-D-glucopyranoside-carbon source
43180potassium 2-dehydro-D-gluconate-carbon source
4318017814salicin-carbon source
4318028017starch-carbon source

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
431802637amikacinyesyes30 µg
4318017698chloramphenicolyesyes30 µg
43180100241ciprofloxacinyesyes5 µg
4318048923erythromycinyesyes15 µg
4318017833gentamicinyesyes10 µg
431806104kanamycinyesyes30 µg
431807528netilmycinyesyes30 µg
4318028368novobiocinyesyes5 µg
4318017334penicillinyesyes10 Unit
4318059062polymyxinyesyes30 Unit
4318017076streptomycinyesyes10 µg
4318027902tetracyclineyesyes30 µg
4318028864tobramycinyesyes10 µg
4318028001vancomycinyesyes30 µg

metabolite production

@refChebi-IDmetaboliteproduction
4318016136hydrogen sulfideno
4318035581indoleno

enzymes

@refvalueactivityec
43180catalase+1.11.1.6
43180cytochrome oxidase-1.9.3.1
43180acid phosphatase+3.1.3.2
43180cystine arylamidase+3.4.11.3
43180esterase (C 4)+
43180esterase Lipase (C 8)+
43180alpha-fucosidase+3.2.1.51
43180beta-galactosidase+3.2.1.23
43180alpha-glucosidase+3.2.1.20
43180leucine arylamidase+3.4.11.1
43180alpha-mannosidase+3.2.1.24
43180N-acetyl-beta-glucosaminidase+3.2.1.52
43180valine arylamidase+
43180alkaline phosphatase-3.1.3.1
43180arginine dihydrolase-3.5.3.6
43180alpha-chymotrypsin-3.4.21.1
43180alpha-galactosidase-3.2.1.22
43180beta-glucosidase-3.2.1.21
43180beta-glucuronidase-3.2.1.31
43180lipase (C 14)-
43180naphthol-AS-BI-phosphohydrolase-
43180trypsin-3.4.21.4
43180urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43180C16:06.1
    43180C11:0 iso3.7
    43180C14:0 iso1.4
    43180C15:0 iso14.3
    43180C16:0 iso19.2
    43180C17:0 iso6.2
    43180C18:0 iso0.9
    43180C15:0 anteiso5.1
    43180C17:0 anteiso1.2
    43180cyclo-C17:01.1
    43180C11:0 iso 3OH4
    43180C13:0 iso 3OH2.3
    43180C17:0 iso 3OH0.5
    43180C15:1ω5c0.2
    43180C15:1ω6c0.8
    43180C17:1 iso ω9c20.4
    43180C15:0 iso 2OH / C16:1ω7c7.6
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 2
  • system: MIS MIDI
  • method/protocol: Kuykendall et al. 1988

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
43180forest soil of Dinghushan Biosphere ReserveGuangdong Province, PR ChinaChinaCHNAsia23112
67771From forest soilDinghushan Biosphere Reserve, Guangdong ProvinceChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil

Sequence information

16S sequences

  • @ref: 43180
  • description: 16S rRNA gene sequences
  • accession: KX430827
  • length: 1468
  • database: ena
  • NCBI tax ID: 1849580

GC content

  • @ref: 43180
  • GC-content: 59.4
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 43180

culture collection no.: NBRC 111473, KCTC 52132

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny28581924Dyella lipolytica sp. nov., a lipolytic bacterium isolated from lower subtropical forest soil.Tang L, Chen MH, Nie XC, Ma MR, Qiu LHInt J Syst Evol Microbiol10.1099/ijsem.0.0020202017Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Forests, Genes, Bacterial, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistry, Xanthomonadaceae/*classification/genetics/isolation & purificationTranscriptome
Phylogeny30124398Dyella halodurans sp. nov., isolated from lower subtropical forest soil.Cai YM, Gao ZH, Chen MH, Huang YX, Qiu LHInt J Syst Evol Microbiol10.1099/ijsem.0.0029692018Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Forests, Genes, Bacterial, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistry, Xanthomonadaceae/*classification/genetics/isolation & purificationTranscriptome
Phylogeny30489237Dyella tabacisoli sp. nov., a bacterium isolated from an arable soil sample of Nicotiana tabacum L.Li QQ, Chen X, Zhou XK, Dong LM, Xiao M, Fang BZ, Li WJ, Duan YQInt J Syst Evol Microbiol10.1099/ijsem.0.0031382019Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Tobacco/*microbiology, Ubiquinone/chemistry, Xanthomonadaceae/*classification/isolation & purificationTranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43180Liang Tang, Mei-hong Chen, Xi-chen Nie, Meng-ran Ma and Li-hong QiuDyella lipolytica sp. nov., a lipolytic bacterium isolated from lower subtropical forest soil10.1099/ijsem.0.002020IJSEM 67: 1235-1240 201728581924
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc