Strain identifier

BacDive ID: 140560

Type strain: Yes

Species: Flavobacterium keumense

Strain Designation: K3R-10

Strain history: <- Seung Bum Kim, Chungnam National Univ.

NCBI tax ID(s): 1306518 (species)

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General

@ref: 43291

BacDive-ID: 140560

keywords: 16S sequence, Bacteria, aerobe, non-halophilic, Gram-negative, rod-shaped, colony-forming

description: Flavobacterium keumense K3R-10 is an aerobe, non-halophilic, Gram-negative bacterium that forms circular colonies and was isolated from Keum River water sample.

NCBI tax id

  • NCBI tax id: 1306518
  • Matching level: species

strain history

@refhistory
67770S. B. Kim; Chungnam Natl. Univ., South Korea; K3R-10.
67771<- Seung Bum Kim, Chungnam National Univ.

doi: 10.13145/bacdive140560.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Flavobacterium
  • species: Flavobacterium keumense
  • full scientific name: Flavobacterium keumense Ekwe et al. 2017

@ref: 43291

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Flavobacterium

species: Flavobacterium keumense

strain designation: K3R-10

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
43291negative1.5-2.0 µm0.5-1.0 µmrod-shapedno
67771rod-shapedno
67771negative

colony morphology

  • @ref: 43291
  • colony size: 1-2 mm
  • colony color: yellow
  • colony shape: circular
  • incubation period: 3 days
  • medium used: R2A Agar (BD)

pigmentation

  • @ref: 43291
  • production: no
  • name: Flexirubin-Type

Culture and growth conditions

culture medium

@refnamegrowth
43291R2A Agar (BD)yes
43291Nutrient agar (Difco)yes
43291Anacker Ordal (Difco)yes
43291R2A-Agar Difcoyes
43291TSA agar (Difco)no
43291MacConkey agar (Difco)no

culture temp

@refgrowthtypetemperaturerange
43291positivegrowth4-35
43291positivemaximum30mesophilic
67770positivegrowth30mesophilic
67771positivegrowth30mesophilic

culture pH

@refabilitytypepH
43291positivegrowth6-8
43291positivemaximum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
43291aerobe
67771aerobe

halophily

@refhalophily levelsaltgrowthtested relationconcentration
43291non-halophilicNaClpositivegrowth0-0.5 %(w/v)
43291non-halophilicNaClnogrowth0.6-5 %(w/v)

observation

@refobservation
67770quinones: MK-6
67771quinones: MK-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4329128017starch+hydrolysis
4329118186tyrosine+hydrolysis
432914853esculin+hydrolysis
432915291gelatin+hydrolysis
432913557154-nitrophenyl beta-D-galactopyranoside+hydrolysis
43291casein-hydrolysis
4329185146carboxymethylcellulose-hydrolysis
4329117029chitin-hydrolysis
4329116991dna-hydrolysis
432912509agar-hydrolysis
4329128757fructose+builds acid from
4329137684mannose+builds acid from
43291320061methyl alpha-D-glucopyranoside+builds acid from
43291506227N-acetylglucosamine+builds acid from
4329127613amygdalin+builds acid from
4329118305arbutin+builds acid from
432914853esculin+builds acid from
4329117814salicin+builds acid from
4329117057cellobiose+builds acid from
4329117306maltose+builds acid from
4329117716lactose+builds acid from
4329128053melibiose+builds acid from
4329117992sucrose+builds acid from
4329127082trehalose+builds acid from
4329115443inulin+builds acid from
432916731melezitose+builds acid from
4329116634raffinose+builds acid from
4329128017starch+builds acid from
4329128087glycogen+builds acid from
4329128066gentiobiose+builds acid from
4329132528turanose+builds acid from

enzymes

@refvalueactivityec
43291catalase-1.11.1.6
43291cytochrome oxidase+1.9.3.1
43291alkaline phosphatase+3.1.3.1
43291esterase+
43291esterase Lipase (C 8)+
43291leucine arylamidase+3.4.11.1
43291cystine arylamidase+3.4.11.3
43291alpha-chymotrypsin+3.4.21.1
43291acid phosphatase+3.1.3.2
43291naphthol-AS-BI-phosphohydrolase+
43291alpha-glucosidase+3.2.1.20
43291beta-glucosidase+3.2.1.21

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43291C14:01.9
    43291C16:03.4
    43291C13:0 iso5.4
    43291C14:0 iso1.4
    43291C15:0 iso18.9
    43291C15:0 iso 3OH21
    43291iso C17:0 3-OH2.1
    43291C15:0 anteiso6.4
    43291iso 15:1 G8.7
    43291C15:1 ω6c2.7
    43291C17:1 ω6c1.1
    43291C15:0 3OH3.1
    43291C16:0 3OH2.6
    43291C12:0 aldehyde / unknown ECL 10.95251.8
    43291C16:1 ω7c / C16:1 ω6c8.9
    43291C16:0 10-methyl / iso-C17:1 ω9c2.6
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A (Difco)
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 14
  • software version: Sherlock 6.1
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperature
43291Keum River water sampleSouth KoreaRepublic of KoreaKORAsiaR2A agar (Difco)7 days30
67770Freshwater source
67771From freshwater, riverRepublic of KoreaKORAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #River (Creek)

taxonmaps

  • @ref: 69479
  • File name: preview.99_18098.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_66;96_352;97_11124;98_13674;99_18098&stattab=map
  • Last taxonomy: Flavobacterium
  • 16S sequence: KC355348
  • Sequence Identity:
  • Total samples: 2451
  • soil counts: 1140
  • aquatic counts: 1147
  • animal counts: 150
  • plant counts: 14

Sequence information

16S sequences

  • @ref: 43291
  • description: Flavobacterium sp. K3R-10 16S ribosomal RNA gene, partial sequence
  • accession: KC355348
  • length: 1440
  • database: nuccore
  • NCBI tax ID: 1306518

GC content

@refGC-contentmethod
4329134.8-36.0thermal denaturation, midpoint method (Tm)
6777035.4thermal denaturation, midpoint method (Tm)
6777135.4

External links

@ref: 43291

culture collection no.: KCTC 52563, JCM 31220

literature

  • topic: Phylogeny
  • Pubmed-ID: 28671526
  • title: Flavobacterium keumense sp. nov., isolated from freshwater.
  • authors: Ekwe AP, Ahn JH, Kim SB
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001920
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, Fresh Water/*microbiology, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Spermidine/chemistry, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43291Adaeze Precious Ekwe, Joong-hyeon Ahn and Seung Bum KimFlavobacterium keumense sp. nov., isolated from freshwater10.1099/ijsem.0.001920IJSEM 67: 2166-2170 201728671526
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/