Name: Flavobacterium keumense K3R-10
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140560

Type strain:

Species: Flavobacterium keumense

Strain Designation: K3R-10

Culture col. no.: KCTC 52563, JCM 31220

Strain history: <- Seung Bum Kim, Chungnam National Univ.

NCBI tax ID(s): 1306518 (species)

Section

Flavobacterium keumense K3R-10 is an aerobe, non-halophilic, Gram-negative bacterium that forms circular colonies and was isolated from Keum River water sample.

colony-forming
Gram-negative
rod-shaped
aerobe
non-halophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Flavobacterium*
	Species	**Flavobacterium keumense**
	Full Scientific Name (LPSN)	Flavobacterium keumense Ekwe et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43291]	Gram stain	negative
[Ref.: #67771]	Gram stain	negative

[Ref.: #43291]	Cell length	1.5-2.0 µm

[Ref.: #43291]	Cell width	0.5-1.0 µm

[Ref.: #43291]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #43291]	Motility	no
[Ref.: #67771]	Motility	no

[Ref.: #43291]	Colony size	1-2 mm
[Ref.: #43291]	Colony color	yellow
[Ref.: #43291]	Colony shape	circular
[Ref.: #43291]	Incubation period	3 days
[Ref.: #43291]	Cultivation medium used	R2A Agar (BD)

[Ref.: #43291]	Pigment production	no
[Ref.: #43291]	Pigment name	Flexirubin-Type

Information on culture and growth conditions Culture and growth conditions

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Culture medium

R2A Agar (BD)

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Culture medium growth

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Culture medium

Nutrient agar (Difco)

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Culture medium growth

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Culture medium

Anacker Ordal (Difco)

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Culture medium growth

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Culture medium

R2A-Agar Difco

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Culture medium growth

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Culture medium

TSA agar (Difco)

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Culture medium growth

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Culture medium

MacConkey agar (Difco)

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Culture medium growth

		Temperature
[Ref.: #43291]	growth	4-35 °C
[Ref.: #43291]	maximum	30 °C
[Ref.: #67770]	growth	30 °C
[Ref.: #67771]	growth	30 °C

[Ref.: #43291]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43291]			maximum	7
[Ref.: #43291]			growth	6-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43291]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #43291]	Halophily / tolerance level	non-halophilic
[Ref.: #43291]	Halophily / tolerance level	non-halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43291]		NaCl		growth	0-0.5	%(w/v)
[Ref.: #43291]		NaCl	no	growth	0.6-5	%(w/v)

[Ref.: #67770]	Observation	quinones: MK-6
[Ref.: #67771]	Observation	quinones: MK-6

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43291]	4-nitrophenyl beta-D-galactopyranoside ChEBI	+	hydrolysis
[Ref.: #43291]	agar ChEBI	-	hydrolysis
[Ref.: #43291]	amygdalin ChEBI	+	builds acid from
[Ref.: #43291]	arbutin ChEBI	+	builds acid from
[Ref.: #43291]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #43291]	casein	-	hydrolysis
[Ref.: #43291]	cellobiose ChEBI	+	builds acid from
[Ref.: #43291]	chitin ChEBI	-	hydrolysis
[Ref.: #43291]	dna ChEBI	-	hydrolysis
[Ref.: #43291]	esculin ChEBI	+	hydrolysis
[Ref.: #43291]	esculin ChEBI	+	builds acid from
[Ref.: #43291]	fructose ChEBI	+	builds acid from
[Ref.: #43291]	gelatin ChEBI	+	hydrolysis
[Ref.: #43291]	gentiobiose ChEBI	+	builds acid from
[Ref.: #43291]	glycogen ChEBI	+	builds acid from
[Ref.: #43291]	inulin ChEBI	+	builds acid from
[Ref.: #43291]	lactose ChEBI	+	builds acid from
[Ref.: #43291]	maltose ChEBI	+	builds acid from
[Ref.: #43291]	mannose ChEBI	+	builds acid from
[Ref.: #43291]	melezitose ChEBI	+	builds acid from
[Ref.: #43291]	melibiose ChEBI	+	builds acid from
[Ref.: #43291]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from
[Ref.: #43291]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #43291]	raffinose ChEBI	+	builds acid from
[Ref.: #43291]	salicin ChEBI	+	builds acid from
[Ref.: #43291]	starch ChEBI	+	hydrolysis
[Ref.: #43291]	starch ChEBI	+	builds acid from
[Ref.: #43291]	sucrose ChEBI	+	builds acid from
[Ref.: #43291]	trehalose ChEBI	+	builds acid from
[Ref.: #43291]	turanose ChEBI	+	builds acid from
[Ref.: #43291]	tyrosine ChEBI	+	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #43291]	acid phosphatase	+	3.1.3.2
[Ref.: #43291]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43291]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #43291]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43291]	beta-glucosidase	+	3.2.1.21
[Ref.: #43291]	catalase	-	1.11.1.6
[Ref.: #43291]	cystine arylamidase	+	3.4.11.3
[Ref.: #43291]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43291]	esterase	+
[Ref.: #43291]	esterase Lipase (C 8)	+
[Ref.: #43291]	leucine arylamidase	+	3.4.11.1
[Ref.: #43291]	naphthol-AS-BI-phosphohydrolase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43291]

Fatty acid	Percentage
C15:0 iso 3OH	21
C15:0 iso	18.9
C16:1 ω7c / C16:1 ω6c	8.9
iso 15:1 G	8.7
C15:0 anteiso	6.4
C13:0 iso	5.4
C16:0	3.4
C15:0 3OH	3.1
C15:1 ω6c	2.7
C16:0 3OH	2.6
C16:0 10-methyl / iso-C17:1 ω9c	2.6
iso C17:0 3-OH	2.1
C14:0	1.9
C12:0 aldehyde / unknown ECL 10.9525	1.8
C14:0 iso	1.4
C17:1 ω6c	1.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43291]	Sample type/isolated from	Keum River water sample
[Ref.: #43291]	Geographic location (country and/or sea, region)	South Korea
[Ref.: #43291]	Country	Republic of Korea
[Ref.: #43291]	Country ISO 3 Code	KOR
[Ref.: #43291]	Continent	Asia
[Ref.: #43291]	Enrichment culture	R2A agar (Difco)
[Ref.: #43291]	Enrichment culture duration	7 days
[Ref.: #43291]	Enrichment culture temperature	30 ̊C

[Ref.: #67770]	Sample type/isolated from	Freshwater source

[Ref.: #67771]	Sample type/isolated from	From freshwater, river
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#River (Creek)

What are isolation sources categories?

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Global distribution of 16S sequence KC355348 (>99% sequence identity) for Flavobacterium from Microbeatlas

Aquatic Samples	1147
Soil Samples	1140
Animal Samples	150
Plant Samples	14
Total Samples	2451

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43291]	Flavobacterium sp. K3R-10 16S ribosomal RNA gene, partial sequence	KC355348	1440	GenBank	1306518 tax ID

[Ref.: #43291]	GC-content	34.8-36.0 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	35.4 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67771]	GC-content	35.4 mol%

Availability in culture collections External links

[Ref.: #43291]

Culture collection no.

KCTC 52563, JCM 31220

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Flavobacterium keumense sp. nov., isolated from freshwater.	Ekwe AP, Ahn JH, Kim SB	Int J Syst Evol Microbiol	10.1099/ijsem.0.001920	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43291

Adaeze Precious Ekwe, Joong-hyeon Ahn and Seung Bum Kim: Flavobacterium keumense sp. nov., isolated from freshwater. IJSEM 67: 2166 - 2170 2017 ( DOI 10.1099/ijsem.0.001920 , PubMed 28671526 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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