Strain identifier

BacDive ID: 140494

Type strain: Yes

Species: Streptomyces adustus

Strain Designation: WH-9, IMER00657

NCBI tax ID(s): 1609272 (species)

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General

@ref: 67669

BacDive-ID: 140494

DSM-Number: 111621

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Streptomyces adustus WH-9 is an aerobe, spore-forming, mesophilic bacterium that builds a substrate mycelium and was isolated from bamboo humus.

NCBI tax id

  • NCBI tax id: 1609272
  • Matching level: species

doi: 10.13145/bacdive140494.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces adustus
  • full scientific name: Streptomyces adustus Lee and Whang 2016

@ref: 67669

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Streptomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces adustus

full scientific name: Streptomyces adustus Lee and Whang 2016

strain designation: WH-9, IMER00657

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
43108positiveno
69480no92.662
69480positive100

multicellular morphology

@refforms multicellular complexcomplex namefurther description
43108yessubstrate myceliumextensively branched
43108yesaerial hyphaedifferentiate into straight to Spirales chains of rough-surfaced spores

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43108ISP 2yes
43108ISP 3yes
43108ISP 4yes
43108ISP 5yes
43108ISP 6yes
43108ISP 7yes
67669GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
67669STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://mediadive.dsmz.de/medium/252Name: STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252) Composition: Agar 14.985 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999001 g/l MgSO4 x 7 H2O 0.999001 g/l NaCl 0.999001 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water
67669ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43108positiveoptimum28mesophilic
43108positivegrowth10-37
67669positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
43108positivegrowth4.0-11.0alkaliphile
43108positiveoptimum6.0-8.0

Physiology and metabolism

oxygen tolerance

  • @ref: 43108
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
43108yes
69481yes100
69480yes100

halophily

@refsaltgrowthtested relationconcentration
43108NaClpositivegrowth0-4 %(w/v)
43108NaClnogrowth5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4310817632nitrate+reduction
43108casein+hydrolysis
4310862968cellulose+hydrolysis
431085291gelatin+hydrolysis
4310828017starch-hydrolysis
4310816199urea-hydrolysis
4310815903beta-d-glucose+carbon source
4310815824D-fructose+carbon source
4310816899D-mannitol+carbon source
4310816634raffinose+carbon source
4310862345L-rhamnose+carbon source
4310817992sucrose+carbon source
4310830849L-arabinose+carbon source
4310865327D-xylose+carbon source

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43108C15:0 anteiso31.32
    43108C15:0 iso11.37
    43108C16:0 iso12.33
  • type of FA analysis: whole cell analysis
  • incubation medium: tryptic soy broth (TSB, Difco)
  • agar/liquid: liquid
  • incubation temperature: 28
  • incubation time: 5
  • software version: Sherlock 4.5
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture durationenrichment culture temperature
43108bamboo (Phyllostachys bambusoides) humusDamyang, JeonnamRepublic of KoreaKORAsiastarch casein agar7 days28
67669Bamboo (Phyllostachys bambusoides) humusDamyang, JeonnamRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Plants#Herbaceous plants (Grass,Crops)

Safety information

risk assessment

  • @ref: 67669
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 43108
  • description: Streptomyces sp. WH-9 gene for 16S ribosomal RNA, partial sequence
  • accession: LC026279
  • length: 1463
  • database: nuccore
  • NCBI tax ID: 1609272

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Streptomyces adustus NBRC 109810GCA_009377175contigncbi1609272
66792Streptomyces adustus strain NBRC 1098101609272.3wgspatric1609272

GC content

  • @ref: 67669
  • GC-content: 74.1
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno95.008no
gram-positiveyes89.333yes
anaerobicno99.164yes
aerobicyes92.298yes
halophileno93.722no
spore-formingyes96.209yes
thermophileno98.89yes
glucose-utilyes90.148yes
flagellatedno98.313yes
glucose-fermentno87.86no

External links

@ref: 67669

culture collection no.: NBRC 109810, KACC 17197, DSM 111621

straininfo link

  • @ref: 96732
  • straininfo: 401329

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27298302Streptomyces rhizosphaerihabitans sp. nov. and Streptomyces adustus sp. nov., isolated from bamboo forest soil.Lee HJ, Whang KSInt J Syst Evol Microbiol10.1099/ijsem.0.0012362016Bacterial Typing Techniques, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Forests, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sasa/*microbiology, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny32139879Streptomyces acidicola sp. nov., isolated from a peat swamp forest in Thailand.Lipun K, Chantavorakit T, Mingma R, Duangmal KJ Antibiot (Tokyo)10.1038/s41429-020-0294-52020Amino Acids, Diamino/genetics, Bacterial Typing Techniques/methods, Base Composition/genetics, DNA, Bacterial/genetics, Diaminopimelic Acid, Fatty Acids/genetics, Forests, Galactose/genetics, Glucose/genetics, Mannose/genetics, Nucleic Acid Hybridization/genetics, Phospholipids/genetics, Phylogeny, RNA, Ribosomal, 16S/genetics, Ribose/genetics, Soil, Soil Microbiology, Streptomyces/genetics/*isolation & purification, Thailand, WetlandsEnzymology

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43108Hyo-Jin Lee, Kyung-Sook WhangStreptomyces rhizosphaerihabitans sp. nov. and Streptomyces adustus sp. nov., isolated from bamboo forest soil10.1099/ijsem.0.001236IJSEM 66: 3573-3578 201627298302
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67669Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-111621Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 111621)
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
96732Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401329.1