Strain identifier

BacDive ID: 140461

Type strain: Yes

Species: Mariniblastus fucicola

Strain Designation: FC18

Strain history: <- O. M. Lage, Faculty Sci., Univ. Porto, Portugal; FC18

NCBI tax ID(s): 980251 (species)

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General

@ref: 66478

BacDive-ID: 140461

DSM-Number: 26290

keywords: genome sequence, 16S sequence, Bacteria, aerobe, chemoheterotroph, mesophilic, sphere-shaped, colony-forming

description: Mariniblastus fucicola FC18 is an aerobe, chemoheterotroph, mesophilic bacterium that forms circular colonies and was isolated from microbial biofilm of the marine macroalga Fucus spiralis.

NCBI tax id

  • NCBI tax id: 980251
  • Matching level: species

strain history

  • @ref: 66478
  • history: <- O. M. Lage, Faculty Sci., Univ. Porto, Portugal; FC18

doi: 10.13145/bacdive140461.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/planctomycetota
  • domain: Bacteria
  • phylum: Planctomycetota
  • class: Planctomycetia
  • order: Pirellulales
  • family: Pirellulaceae
  • genus: Mariniblastus
  • species: Mariniblastus fucicola
  • full scientific name: Mariniblastus fucicola Lage et al. 2017

@ref: 66478

domain: Bacteria

phylum: Planctomycetes

class: Planctomycetia

order: Pirellulales

family: Pirellulaceae

genus: Mariniblastus

species: Mariniblastus fucicola

full scientific name: Mariniblastus fucicola Lage et al. 2017

strain designation: FC18

type strain: yes

Morphology

cell morphology

  • @ref: 43193
  • cell length: 2 µm
  • cell width: 1 µm
  • cell shape: sphere-shaped
  • motility: no

colony morphology

@refcolony colorcolony shapemedium used
43193whitecircularM13 agar
43193light-pinkcircularM14 agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43193M13 agaryes
43193M14 agaryes
66478MODIFIED M13 VERRUCOMICROBIUM MEDIUM (DSMZ Medium 1601)yeshttps://mediadive.dsmz.de/medium/1601Name: MODIFIED M13 VERRUCOMICROBIUM MEDIUM (DSMZ Medium 1601) Composition: MgSO4 x 7 H2O 0.594 g/l Peptone 0.25 g/l Yeast extract 0.25 g/l Glucose 0.25 g/l Nitrilotriacetic acid 0.2 g/l CaCl2 x 2 H2O 0.0667 g/l ZnSO4 x 7 H2O 0.001095 g/l FeSO4 x 7 H2O 0.0005 g/l Na-EDTA 0.00025 g/l (NH4)6Mo7O24 x 4 H2O 0.000185 g/l MnSO4 x H2O 0.000154 g/l Riboflavin 5e-05 g/l Calcium pantothenate 5e-05 g/l Nicotinamide 5e-05 g/l Thiamine-HCl x 2 H2O 5e-05 g/l CuSO4 x 5 H2O 3.92e-05 g/l Co(NO3)2 x 6 H2O 2.48e-05 g/l Folic acid 2e-05 g/l Biotin 2e-05 g/l Na2B4O7 x 10 H2O 1.77e-05 g/l Vitamin B12 1e-06 g/l Tris-HCl buffer Sea water Distilled water

culture temp

@refgrowthtypetemperaturerange
43193positiveoptimum25mesophilic
43193positivegrowth10-30
66478positivegrowth25mesophilic

culture pH

@refabilitytypepHPH range
43193positivegrowth6.5-8.5alkaliphile
43193positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 43193
  • oxygen tolerance: aerobe

nutrition type

  • @ref: 43193
  • type: chemoheterotroph

halophily

@refsaltgrowthtested relationconcentration
43193NaClpositivegrowth0.5-5.0 %
43193NaClpositiveoptimum2.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
431935291gelatin+hydrolysis
431934853esculin+hydrolysis
4319318305arbutin-degradation
4319328017starch-degradation
4319337166xylan-degradation
4319353424tween 20-degradation
4319353426tween 80-degradation
4319317632nitrate+reduction
4319317634D-glucose+assimilation
4319315824D-fructose+assimilation
4319312936D-galactose+assimilation
4319362345L-rhamnose+assimilation
4319365327D-xylose+assimilation
4319316634raffinose+assimilation
4319317992sucrose+assimilation
4319317306maltose+assimilation
4319317716lactose+assimilation
4319317057cellobiose+assimilation
4319327082trehalose+assimilation
43193506227N-acetylglucosamine+assimilation
4319316899D-mannitol+assimilation
4319315361pyruvate+assimilation
43193casamino acids+assimilation
4319318287L-fucose-assimilation
4319317266L-sorbose-assimilation
4319316988D-ribose-assimilation
4319317108D-arabinose-assimilation
4319330849L-arabinose-assimilation
4319315443inulin-assimilation
4319317754glycerol-assimilation
4319315963ribitol-assimilation
4319330911sorbitol-assimilation
4319317268myo-inositol-assimilation
4319317113erythritol-assimilation
4319318333D-arabitol-assimilation
43193309162-oxoglutarate-assimilation
4319330089acetate-assimilation
4319373706bromosuccinate-assimilation
4319325115malate-assimilation
4319316947citrate-assimilation
4319316150benzoate-assimilation
4319329806fumarate-assimilation
4319315740formate-assimilation
43193506227N-acetylglucosamine+nitrogen source
43193casamino acids+nitrogen source
4319328938ammonium+nitrogen source
43193peptone+nitrogen source
4319317632nitrate+nitrogen source
4319335391aspartate+nitrogen source
4319329985L-glutamate+nitrogen source
4319316977L-alanine+nitrogen source
4319317203L-proline+nitrogen source
4319318050L-glutamine+nitrogen source
4319316199urea-nitrogen source
4319316301nitrite-nitrogen source
4319317196L-asparagine-nitrogen source
4319315971L-histidine-nitrogen source
4319318019L-lysine-nitrogen source
4319316467L-arginine-nitrogen source
4319316414L-valine-nitrogen source
4319317295L-phenylalanine-nitrogen source
4319316643L-methionine-nitrogen source
4319316857L-threonine-nitrogen source
4319315356cysteine-nitrogen source
4319317376cystine-nitrogen source
4319318186tyrosine-nitrogen source
4319327897tryptophan-nitrogen source

enzymes

@refvalueactivityec
43193alkaline phosphatase+3.1.3.1
43193esterase (C 4)-
43193esterase Lipase (C 8)-
43193leucine arylamidase+3.4.11.1
43193valine arylamidase+
43193cystine arylamidase-3.4.11.3
43193trypsin-3.4.21.4
43193acid phosphatase+3.1.3.2
43193naphthol-AS-BI-phosphohydrolase+
43193alpha-galactosidase-3.2.1.22
43193beta-galactosidase-3.2.1.23
43193beta-glucuronidase-3.2.1.31
43193beta-glucosidase-3.2.1.21
43193N-acetyl-beta-glucosaminidase-3.2.1.52
43193alpha-fucosidase-3.2.1.51
43193lipase (C 14)-
43193alpha-chymotrypsin-3.4.21.1
43193alpha-glucosidase+3.2.1.20
43193alpha-mannosidase-3.2.1.24
43193arginine dihydrolase+3.5.3.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentageSDECL
    43193Unknown0.50.111970
    43193C14:021.70.514000
    43193C15:0 iso1.20.214623
    43193C 16:1ω6c/C16:1ω7c/iso-C15:0 2-OH5.80.315822
    43193C16:021.10.516
    43193C18:1ω9c36.70.817769
    43193C18:1ω6c / C18:1ω7c2.10.217823
    43193C18:08.90.418
    43193Unknown0.80.120
  • type of FA analysis: whole cell analysis
  • incubation medium: M13 medium
  • agar/liquid: liquid
  • incubation temperature: 26
  • system: MIS MIDI
  • method/protocol: Da Costa et al. 2011

Isolation, sampling and environmental information

isolation

  • @ref: 43193
  • sample type: microbial biofilm of the marine macroalga Fucus spiralis
  • geographic location: Carreço, northern coast
  • country: Portugal
  • origin.country: PRT
  • continent: Europe
  • latitude: 41
  • longitude: 8
  • enrichment culture: HEP-PSO-buffered M13 medium

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Biofilm
#Host#Microbial

taxonmaps

  • @ref: 69479
  • File name: preview.99_4065.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_442;96_2063;97_2488;98_3079;99_4065&stattab=map
  • Last taxonomy: Mariniblastus fucicola subclade
  • 16S sequence: HQ845450
  • Sequence Identity:
  • Total samples: 2450
  • soil counts: 51
  • aquatic counts: 2357
  • animal counts: 18
  • plant counts: 24

Safety information

risk assessment

  • @ref: 66478
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 43193
  • description: Planctomycetaceae bacterium FC18 16S ribosomal RNA gene, partial sequence
  • accession: HQ845450
  • length: 1439
  • database: nuccore
  • NCBI tax ID: 980251

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mariniblastus fucicola FC18GCA_008087665completencbi980251
66792Mariniblastus fucicola FC18GCA_001642875contigncbi980251
66792Mariniblastus fucicola strain FC18980251.5completepatric980251

GC content

  • @ref: 66478
  • GC-content: 54.2
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes65.982yes
flagellatedno83.725yes
gram-positiveno98.28no
anaerobicno97.859yes
aerobicyes88.081yes
halophileyes59.39no
spore-formingno94.539no
glucose-utilyes90.899yes
thermophileno98.078yes
glucose-fermentno86.18no

External links

@ref: 66478

culture collection no.: LMG 29748, DSM 26290

straininfo link

  • @ref: 96724
  • straininfo: 407730

literature

  • topic: Phylogeny
  • Pubmed-ID: 28036240
  • title: Mariniblastus fucicola gen. nov., sp. nov. a novel planctomycete associated with macroalgae.
  • authors: Lage OM, Albuquerque L, Lobo-da Cunha A, da Costa MS
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001760
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phosphatidylglycerols/chemistry, *Phylogeny, Planctomycetales/*classification/genetics/isolation & purification, Portugal, RNA, Ribosomal, 16S/genetics, Seaweed/*microbiology, Sequence Analysis, DNA, Vitamin K 2/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
43193Olga Maria Lage, Luciana Albuquerque, Alexandre Lobo-da Cunha, Milton S. da CostaMariniblastus fucicola gen. nov., sp. nov. a novel planctomycete associated with macroalgae10.1099/ijsem.0.001760IJSEM 67: 1571-1576 201728036240
66478Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-26290Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26290)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
96724Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID407730.1