Strain identifier

BacDive ID: 140343

Type strain: Yes

Species: Naumannella cuiyingiana

Strain Designation: AFT2

Strain history: CCTCC AB 2015428 <-- H. Feng; Sch. of Life Sci., Lanzhou Univ., China; AFT2.

NCBI tax ID(s): 1347891 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 8.1 (current version)

General

@ref: 42950

BacDive-ID: 140343

DSM-Number: 103164

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-positive, oval-shaped, colony-forming

description: Naumannella cuiyingiana AFT2 is an aerobe, Gram-positive, oval-shaped bacterium that forms circular colonies and was isolated from mural painting of a 1500-year-tomb.

NCBI tax id

  • NCBI tax id: 1347891
  • Matching level: species

strain history

@refhistory
42950<- H. Feng, School of Life Sci., Lanzhou Univ., China; AFT2
67770CCTCC AB 2015428 <-- H. Feng; Sch. of Life Sci., Lanzhou Univ., China; AFT2.

doi: 10.13145/bacdive140343.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Propionibacteriaceae
  • genus: Naumannella
  • species: Naumannella cuiyingiana
  • full scientific name: Naumannella cuiyingiana Tian et al. 2017

@ref: 42950

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Propionibacteriaceae

genus: Naumannella

species: Naumannella cuiyingiana

full scientific name: Naumannella cuiyingiana Tian et al. 2017

strain designation: AFT2

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
43844positive0.6-1.0 µm0.3-0.7 µmoval-shapedno
69480positive100

colony morphology

  • @ref: 43844
  • colony size: 2 mm
  • colony color: white
  • colony shape: circular
  • incubation period: 3 days
  • medium used: Tryptic soy agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43844Tryptic soy agaryes
42950TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43844positivegrowth20-37
42950positivegrowth28mesophilic
67770positivegrowth28mesophilic

culture pH

  • @ref: 43844
  • ability: positive
  • type: growth
  • pH: 6.0-10.0
  • PH range: alkaliphile

Physiology and metabolism

oxygen tolerance

  • @ref: 43844
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
43844no
69481yes90
69480no98.808

halophily

  • @ref: 43844
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-12 %(w/v)

observation

@refobservation
43844quinone MK-9
67770quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4384417128adipate-assimilation
4384422599arabinose-assimilation
4384427689decanoate-assimilation
4384416947citrate-assimilation
4384418401phenylacetate-assimilation
43844168082-dehydro-D-gluconate-builds acid from
43844581435-dehydro-D-gluconate-builds acid from
4384415963ribitol-builds acid from
4384427613amygdalin-builds acid from
4384418305arbutin-builds acid from
4384417057cellobiose-builds acid from
4384418333D-arabitol-builds acid from
4384415824D-fructose-builds acid from
4384412936D-galactose-builds acid from
4384417634D-glucose-builds acid from
4384462318D-lyxose-builds acid from
4384416899D-mannitol-builds acid from
4384416024D-mannose-builds acid from
4384416988D-ribose-builds acid from
4384417924D-sorbitol-builds acid from
4384416443D-tagatose-builds acid from
4384465327D-xylose-builds acid from
4384416813galactitol-builds acid from
4384417113erythritol-builds acid from
4384424265gluconate-builds acid from
4384417754glycerol-builds acid from
4384428087glycogen-builds acid from
4384417268myo-inositol-builds acid from
4384415443inulin-builds acid from
4384430849L-arabinose-builds acid from
4384418403L-arabitol-builds acid from
4384465328L-xylose-builds acid from
4384424996lactate-builds acid from
4384417306maltose-builds acid from
438446731melezitose-builds acid from
4384428053melibiose-builds acid from
43844320061methyl alpha-D-glucopyranoside-builds acid from
4384443943methyl alpha-D-mannoside-builds acid from
4384474863methyl beta-D-xylopyranoside-builds acid from
43844506227N-acetylglucosamine-builds acid from
4384416634raffinose-builds acid from
4384417814salicin-builds acid from
4384427922sorbose-builds acid from
4384428017starch-builds acid from
4384417992sucrose-builds acid from
4384427082trehalose-builds acid from
4384432528turanose-builds acid from
4384417151xylitol-builds acid from
43844309162-oxoglutarate-carbon source
43844620642,3-butanediol-carbon source
4384416335adenosine-carbon source
4384440585alpha-cyclodextrin-carbon source
4384417925alpha-D-glucose-carbon source
4384416027amp-carbon source
4384427613amygdalin-carbon source
4384423652dextrin-carbon source
4384429042glucose 1-phosphate-carbon source
4384417754glycerol-carbon source
4384414336glycerol 1-phosphate-carbon source
4384428087glycogen-carbon source
4384417596inosine-carbon source
4384417268myo-inositol-carbon source
4384416977L-alanine-carbon source
4384473786L-alanylglycine-carbon source
4384418183L-pyroglutamic acid-carbon source
4384475144lactamide-carbon source
4384428808mannan-carbon source
43844320055methyl beta-D-glucopyranoside-carbon source
4384444337N-acetyl-L-glutamate-carbon source
4384415361pyruvate-carbon source
4384417992sucrose-carbon source
4384417748thymidine-carbon source
4384463528thymidine 5'-monophosphate-carbon source
4384416704uridine-carbon source
43844casein-hydrolysis
4384417632nitrate-reduction
4384417234glucose+assimilation
4384425115malate+assimilation
4384417306maltose+assimilation
4384429864mannitol+assimilation
4384437684mannose+assimilation
43844506227N-acetylglucosamine+assimilation
4384417108D-arabinose+builds acid from
438444853esculin+builds acid from
4384462345L-rhamnose+builds acid from
43844172562-deoxyadenosine+carbon source
43844739183-O-methyl-D-glucose+carbon source
4384418305arbutin+carbon source
43844495055beta-cyclodextrin+carbon source
4384415570D-alanine+carbon source
4384417108D-arabinose+carbon source
4384415824D-fructose+carbon source
4384478697D-fructose 6-phosphate+carbon source
4384412936D-galactose+carbon source
4384418024D-galacturonic acid+carbon source
4384418391D-gluconate+carbon source
4384417634D-glucose+carbon source
4384414314D-glucose 6-phosphate+carbon source
4384416899D-mannitol+carbon source
4384416024D-mannose+carbon source
4384427605D-psicose+carbon source
4384416988D-ribose+carbon source
4384417924D-sorbitol+carbon source
4384428066gentiobiose+carbon source
4384415443inulin+carbon source
4384430849L-arabinose+carbon source
4384429991L-aspartate+carbon source
4384429985L-glutamate+carbon source
4384462345L-rhamnose+carbon source
4384417115L-serine+carbon source
4384424996lactate+carbon source
438446359lactulose+carbon source
4384417306maltose+carbon source
4384461993maltotriose+carbon source
438446731melezitose+carbon source
4384428053melibiose+carbon source
4384455507methyl alpha-D-galactoside+carbon source
43844320061methyl alpha-D-glucopyranoside+carbon source
4384443943methyl alpha-D-mannoside+carbon source
4384417540methyl beta-D-galactoside+carbon source
43844506227N-acetylglucosamine+carbon source
4384473784glycyl-l-glutamate+carbon source
4384417148putrescine+carbon source
4384416634raffinose+carbon source
4384417814salicin+carbon source
4384417164stachyose+carbon source
4384427082trehalose+carbon source
4384453423tween 40+carbon source
4384453426tween 80+carbon source
4384416695uridine 5'-monophosphate+carbon source
438444853esculin+hydrolysis
438445291gelatin+hydrolysis
4384428017starch+hydrolysis
4384424265gluconate+/-assimilation

metabolite production

  • @ref: 43844
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
43844catalase+1.11.1.6
43844beta-galactosidase+3.2.1.23
43844alkaline phosphatase+3.1.3.1
43844esterase (C 4)+
43844esterase Lipase (C 8)+
43844leucine arylamidase+3.4.11.1
43844valine arylamidase+
43844cystine arylamidase+3.4.11.3
43844naphthol-AS-BI-phosphohydrolase+
43844alpha-glucosidase+3.2.1.20
43844alpha-mannosidase+3.2.1.24
43844alpha-galactosidase+3.2.1.22
43844urease-3.5.1.5
43844arginine dihydrolase-3.5.3.6
43844lipase (C 14)-
43844trypsin-3.4.21.4
43844chymotrypsin-3.4.4.5
43844N-acetyl-beta-glucosaminidase-3.2.1.52
43844beta-D-fucosidase-3.2.1.38
43844beta-glucuronidase-3.2.1.31
43844acid phosphatase+/-3.1.3.2

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43844C15:0 anteiso55.32
    43844C17:0 anteiso9.33
    43844C16:04.37
    43844C16:1ω7c / C16:1ω6c3.22
    43844C17:01.6
    43844C18:02.04
    43844C18:1ω7c / C18:1ω6c1.29
    43844C18:1ω9c3.18
    43844C15:0 iso6.55
    43844C16:0 iso5.12
    43844C17:0 iso1.27
    43844C17:0 iso 3OH1.03
  • type of FA analysis: whole cell analysis
  • incubation medium: Tryptic soy agar
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 1
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • instrument: Hewlett Packard 6890 N gas chromatograph
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
42950mural painting of a 1500-year-tombXianxiu Xu's grave, TaiyuanChinaCHNAsia37.8366112.612
43844mural painting sample from ca. 1500-year-old tomb located in the Northern Qi Dynasty in Shanxi Province, ChinaShanxi ProvinceChinaCHNAsiaPYGV liquid mediumPeptone (0.025%, w/v), yeast extract (0.025%, w/v), glucose (0.025%, w/v), 20 ml per litre of mineral salt solution [10 g/L nitrilotriacetic acid, 29.7 g/L MgSO4.7H2O, 3.34 g/L CaCl2.2H2O, 12.67 mg/L Na2MoO4.2H2O, 99 mg/L FeSO4.7H2O and 50 ml metal salt solution (250 mg sodium EDTA, 1095 mg ZnSO4.7H2O, 500 mg/L FeSO4.7H2O, 154 mg/L MnSO4.H2O, 39.2 mg/L CuSO4.5H2O, 24.8 mg/L Co(NO3)2.6H2O and 17.7 mg/L Na2B4O7.10H2O), pH 7.2] and 5 ml per litre of vitamin solution (4 mg/L biotin, 4 mg/L15 days20
67770Mural painting sample from ca. 1500-year-old tombChinaCHNAsia

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Other
  • Cat3: #Painting

taxonmaps

  • @ref: 69479
  • File name: preview.99_20049.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_72;96_10225;97_12335;98_15162;99_20049&stattab=map
  • Last taxonomy: Naumannella
  • 16S sequence: KF041479
  • Sequence Identity:
  • Total samples: 1338
  • soil counts: 288
  • aquatic counts: 264
  • animal counts: 744
  • plant counts: 42

Safety information

risk assessment

  • @ref: 42950
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 42950
  • description: Naumannella cuiyingiana strain AFT2 16S ribosomal RNA gene, partial sequence
  • accession: KF041479
  • length: 1484
  • database: ena
  • NCBI tax ID: 1347891

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Naumannella cuiyingiana strain DSM 1031641347891.3wgspatric1347891
66792Naumannella cuiyingiana DSM 1031642834019907draftimg1347891
67770Naumannella cuiyingiana DSM 103164GCA_013408305contigncbi1347891

GC content

@refGC-contentmethod
4384471.97high performance liquid chromatography (HPLC)
4295071.97

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes90no
motileno94.39no
gram-positiveyes87.951yes
anaerobicno99.45no
halophileno80.758no
spore-formingno85.898yes
thermophileno97.307yes
glucose-utilyes84.02no
aerobicyes93.244yes
flagellatedno98.458no
glucose-fermentno85.811no

External links

@ref: 42950

culture collection no.: DSM 103164, CCTCC AB 2015428, JCM 33579

straininfo link

  • @ref: 96642
  • straininfo: 401338

literature

  • topic: Phylogeny
  • Pubmed-ID: 28792371
  • title: Description of Naumannella cuiyingiana sp. nov., isolated from a ca. 1500-year-old mural painting, and emended description of the genus Naumannella.
  • authors: Tian T, Wu F, Ma Y, Xiang T, Ma W, Hu W, Wu G, An L, Wang W, Feng H
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001964
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, Cemeteries, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, *Paintings, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, Propionibacteriaceae/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
42950Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-103164Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103164)
43844Tian Tian, Fasi Wu, Yantian Ma, Ting Xiang, Wenxia Ma, Weigang Hu, Guangwen Wu, Lizhe An, Wanfu Wang, Huyuan FengDescription of Naumannella cuiyingiana sp. nov., isolated from a ca. 1500-year-old mural painting, and emended description of the genus Naumannella10.1099/ijsem.0.001964IJSEM 67: 2609-2614 201728792371
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
96642Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID401338.1