Naumannella cuiyingiana (DSM 103164, CCTCC AB 2015428, AFT2)

Name: Naumannella cuiyingiana (DSM 103164, CCTCC AB 2015428, AFT2)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 140343

Type strain

Strain Designation AFT2

Culture col. no. DSM 103164 CCTCC AB 2015428 AFT2 JCM 33579

NCBI tax ID(s) 1347891

Special Collections DSMZ JCM Literature strains

History DSMZ <- H. Feng, School of Life Sci., Lanzhou Univ., China; AFT2 JCM <-- CCTCC AB 2015428 <-- H. Feng; Sch. of Life Sci., Lanzhou Univ., China; AFT2.

Links

Naumannella cuiyingiana AFT2 is an aerobe, Gram-positive, oval-shaped bacterium that forms circular colonies and was isolated from mural painting of a 1500-year-tomb.

Gram-positive oval-shaped colony-forming aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 401338

DSM 103164

@ref 20215

Last LPSN update 2026-05-29 08:39:41

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Propionibacteriales

Family Propionibacteriaceae

Genus Naumannella

Species Naumannella cuiyingiana

Full scientific name Naumannella cuiyingiana Tian et al. 2017

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
43844	positive	0.6-1.0 µm	0.3-0.7 µm	oval-shaped
125438					94.5
125438	positive				91.374

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
43844	2 mm	white	circular	3 days	Tryptic soy agar

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
43844	Tryptic soy agar
42950	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)		Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
42950	positive	growth	28
43844	positive	growth	20-37
67770	positive	growth	28

Culture pH

@ref	Ability	Type	PH	PH range
43844	positive	growth	6.0-10.0	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
43844	aerobe
125438	aerobe	90.038
125439	obligate aerobe	99.374

Spore formation

43844

Spore formationno

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43844	NaCl	positive	growth	0-12 %(w/v)

Observation

@ref	Observation
43844	quinone MK-9
67770	quinones: MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43844	62064 ChEBI	2,3-butanediol	-	carbon source
43844	16808 ChEBI	2-dehydro-D-gluconate	-	builds acid from
43844	17256 ChEBI	2-deoxyadenosine	+	carbon source
43844	30916 ChEBI	2-oxoglutarate	-	carbon source
43844	73918 ChEBI	3-O-methyl-D-glucose	+	carbon source
43844	58143 ChEBI	5-dehydro-D-gluconate	-	builds acid from
43844	16335 ChEBI	adenosine	-	carbon source
43844	17128 ChEBI	adipate	-	assimilation
43844	40585 ChEBI	alpha-cyclodextrin	-	carbon source
43844	17925 ChEBI	alpha-D-glucose	-	carbon source
43844	16027 ChEBI	amp	-	carbon source
43844	27613 ChEBI	amygdalin	-	builds acid from
43844	27613 ChEBI	amygdalin	-	carbon source
43844	22599 ChEBI	arabinose	-	assimilation
43844	18305 ChEBI	arbutin	-	builds acid from
43844	18305 ChEBI	arbutin	+	carbon source
43844	495055 ChEBI	beta-cyclodextrin	+	carbon source
43844		casein	-	hydrolysis
43844	17057 ChEBI	cellobiose	-	builds acid from
43844	16947 ChEBI	citrate	-	assimilation
43844	15570 ChEBI	D-alanine	+	carbon source
43844	17108 ChEBI	D-arabinose	+	builds acid from
43844	17108 ChEBI	D-arabinose	+	carbon source
43844	18333 ChEBI	D-arabitol	-	builds acid from
43844	15824 ChEBI	D-fructose	-	builds acid from
43844	15824 ChEBI	D-fructose	+	carbon source
43844	78697 ChEBI	D-fructose 6-phosphate	+	carbon source
43844	12936 ChEBI	D-galactose	-	builds acid from
43844	12936 ChEBI	D-galactose	+	carbon source
43844	18024 ChEBI	D-galacturonic acid	+	carbon source
43844	18391 ChEBI	D-gluconate	+	carbon source
43844	17634 ChEBI	D-glucose	-	builds acid from
43844	17634 ChEBI	D-glucose	+	carbon source
43844	14314 ChEBI	D-glucose 6-phosphate	+	carbon source
43844	62318 ChEBI	D-lyxose	-	builds acid from
43844	16899 ChEBI	D-mannitol	-	builds acid from
43844	16899 ChEBI	D-mannitol	+	carbon source
43844	16024 ChEBI	D-mannose	-	builds acid from
43844	16024 ChEBI	D-mannose	+	carbon source
43844	27605 ChEBI	D-psicose	+	carbon source
43844	16988 ChEBI	D-ribose	-	builds acid from
43844	16988 ChEBI	D-ribose	+	carbon source
43844	17924 ChEBI	D-sorbitol	-	builds acid from
43844	17924 ChEBI	D-sorbitol	+	carbon source
43844	16443 ChEBI	D-tagatose	-	builds acid from
43844	65327 ChEBI	D-xylose	-	builds acid from
43844	27689 ChEBI	decanoate	-	assimilation
43844	23652 ChEBI	dextrin	-	carbon source
43844	17113 ChEBI	erythritol	-	builds acid from
43844	4853 ChEBI	esculin	+	builds acid from
43844	4853 ChEBI	esculin	+	hydrolysis
43844	16813 ChEBI	galactitol	-	builds acid from
43844	5291 ChEBI	gelatin	+	hydrolysis
43844	28066 ChEBI	gentiobiose	+	carbon source
43844	24265 ChEBI	gluconate	-	builds acid from
43844	24265 ChEBI	gluconate	+/-	assimilation
43844	17234 ChEBI	glucose	+	assimilation
43844	29042 ChEBI	glucose 1-phosphate	-	carbon source
43844	17754 ChEBI	glycerol	-	builds acid from
43844	17754 ChEBI	glycerol	-	carbon source
43844	14336 ChEBI	glycerol 1-phosphate	-	carbon source
43844	28087 ChEBI	glycogen	-	builds acid from
43844	28087 ChEBI	glycogen	-	carbon source
43844	73784 ChEBI	glycyl-l-glutamate	+	carbon source
43844	17596 ChEBI	inosine	-	carbon source
43844	15443 ChEBI	inulin	-	builds acid from
43844	15443 ChEBI	inulin	+	carbon source
43844	16977 ChEBI	L-alanine	-	carbon source
43844	73786 ChEBI	L-alanylglycine	-	carbon source
43844	30849 ChEBI	L-arabinose	-	builds acid from
43844	30849 ChEBI	L-arabinose	+	carbon source
43844	18403 ChEBI	L-arabitol	-	builds acid from
43844	29991 ChEBI	L-aspartate	+	carbon source
43844	29985 ChEBI	L-glutamate	+	carbon source
43844	18183 ChEBI	L-pyroglutamic acid	-	carbon source
43844	62345 ChEBI	L-rhamnose	+	builds acid from
43844	62345 ChEBI	L-rhamnose	+	carbon source
43844	17115 ChEBI	L-serine	+	carbon source
43844	65328 ChEBI	L-xylose	-	builds acid from
43844	75144 ChEBI	lactamide	-	carbon source
43844	24996 ChEBI	lactate	-	builds acid from
43844	24996 ChEBI	lactate	+	carbon source
43844	6359 ChEBI	lactulose	+	carbon source
43844	25115 ChEBI	malate	+	assimilation
43844	17306 ChEBI	maltose	-	builds acid from
43844	17306 ChEBI	maltose	+	assimilation
43844	17306 ChEBI	maltose	+	carbon source
43844	61993 ChEBI	maltotriose	+	carbon source
43844	28808 ChEBI	mannan	-	carbon source
43844	29864 ChEBI	mannitol	+	assimilation
43844	37684 ChEBI	mannose	+	assimilation
43844	6731 ChEBI	melezitose	-	builds acid from
43844	6731 ChEBI	melezitose	+	carbon source
43844	28053 ChEBI	melibiose	-	builds acid from
43844	28053 ChEBI	melibiose	+	carbon source
43844	55507 ChEBI	methyl alpha-D-galactoside	+	carbon source
43844	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from
43844	320061 ChEBI	methyl alpha-D-glucopyranoside	+	carbon source
43844	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from
43844	43943 ChEBI	methyl alpha-D-mannoside	+	carbon source
43844	17540 ChEBI	methyl beta-D-galactoside	+	carbon source
43844	320055 ChEBI	methyl beta-D-glucopyranoside	-	carbon source
43844	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from
43844	17268 ChEBI	myo-inositol	-	builds acid from
43844	17268 ChEBI	myo-inositol	-	carbon source
43844	44337 ChEBI	N-acetyl-L-glutamate	-	carbon source
43844	506227 ChEBI	N-acetylglucosamine	-	builds acid from
43844	506227 ChEBI	N-acetylglucosamine	+	assimilation
43844	506227 ChEBI	N-acetylglucosamine	+	carbon source
43844	17632 ChEBI	nitrate	-	reduction
43844	18401 ChEBI	phenylacetate	-	assimilation
43844	17148 ChEBI	putrescine	+	carbon source
43844	15361 ChEBI	pyruvate	-	carbon source
43844	16634 ChEBI	raffinose	-	builds acid from
43844	16634 ChEBI	raffinose	+	carbon source
43844	15963 ChEBI	ribitol	-	builds acid from
43844	17814 ChEBI	salicin	-	builds acid from
43844	17814 ChEBI	salicin	+	carbon source
43844	27922 ChEBI	sorbose	-	builds acid from
43844	17164 ChEBI	stachyose	+	carbon source
43844	28017 ChEBI	starch	-	builds acid from
43844	28017 ChEBI	starch	+	hydrolysis
43844	17992 ChEBI	sucrose	-	builds acid from
43844	17992 ChEBI	sucrose	-	carbon source
43844	17748 ChEBI	thymidine	-	carbon source
43844	63528 ChEBI	thymidine 5'-monophosphate	-	carbon source
43844	27082 ChEBI	trehalose	-	builds acid from
43844	27082 ChEBI	trehalose	+	carbon source
43844	32528 ChEBI	turanose	-	builds acid from
43844	53423 ChEBI	tween 40	+	carbon source
43844	53426 ChEBI	tween 80	+	carbon source
43844	16704 ChEBI	uridine	-	carbon source
43844	16695 ChEBI	uridine 5'-monophosphate	+	carbon source
43844	17151 ChEBI	xylitol	-	builds acid from

Metabolite production

@ref	Chebi-ID	Metabolite	Production
43844	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
43844	acid phosphatase	+/-	3.1.3.2
43844	alkaline phosphatase	+	3.1.3.1
43844	alpha-galactosidase	+	3.2.1.22
43844	alpha-glucosidase	+	3.2.1.20
43844	alpha-mannosidase	+	3.2.1.24
43844	arginine dihydrolase	-	3.5.3.6
43844	beta-D-fucosidase	-	3.2.1.38
43844	beta-galactosidase	+	3.2.1.23
43844	beta-glucuronidase	-	3.2.1.31
43844	catalase	+	1.11.1.6
43844	chymotrypsin	-	3.4.4.5
43844	cystine arylamidase	+	3.4.11.3
43844	esterase (C 4)	+
43844	esterase Lipase (C 8)	+
43844	leucine arylamidase	+	3.4.11.1
43844	lipase (C 14)	-
43844	N-acetyl-beta-glucosaminidase	-	3.2.1.52
43844	naphthol-AS-BI-phosphohydrolase	+
43844	trypsin	-	3.4.21.4
43844	urease	-	3.5.1.5
43844	valine arylamidase	+

Fatty acid profile

Metadata FA analysis

@ref

43844

Fatty acid	Percentage
C15:0 anteiso	55.32
C17:0 anteiso	9.33
C15:0 iso	6.55
C16:0 iso	5.12
C16:0	4.37
C16:1ω7c / C16:1ω6c	3.22
C18:1ω9c	3.18
C18:0	2.04
C17:0	1.6
C18:1ω7c / C18:1ω6c	1.29
C17:0 iso	1.27
C17:0 iso 3OH	1.03

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Other	#Painting

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture composition	Enrichment culture duration	Enrichment culture temperature	Latitude	Longitude
42950	mural painting of a 1500-year-tomb	Xianxiu Xu's grave, Taiyuan	China	CHN	Asia					37.8366	112.612 37.8366/112.612
43844	mural painting sample from ca. 1500-year-old tomb located in the Northern Qi Dynasty in Shanxi Province, China	Shanxi Province	China	CHN	Asia	PYGV liquid medium	Peptone (0.025%, w/v), yeast extract (0.025%, w/v), glucose (0.025%, w/v), 20 ml per litre of mineral salt solution [10 g/L nitrilotriacetic acid, 29.7 g/L MgSO4.7H2O, 3.34 g/L CaCl2.2H2O, 12.67 mg/L Na2MoO4.2H2O, 99 mg/L FeSO4.7H2O and 50 ml metal salt solution (250 mg sodium EDTA, 1095 mg ZnSO4.7H2O, 500 mg/L FeSO4.7H2O, 154 mg/L MnSO4.H2O, 39.2 mg/L CuSO4.5H2O, 24.8 mg/L Co(NO3)2.6H2O and 17.7 mg/L Na2B4O7.10H2O), pH 7.2] and 5 ml per litre of vitamin solution (4 mg/L biotin, 4 mg/L	15 days	20
67770	Mural painting sample from ca. 1500-year-old tomb		China	CHN	Asia

Taxonmaps

69479

Global distribution of 16S sequence KF041479 (>99% sequence identity) for Naumannella from Microbeatlas

Aquatic Samples	264
Soil Samples	288
Animal Samples	744
Plant Samples	42
Total Samples	1338

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
42950	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM1340830v1 assembly for Naumannella cuiyingiana DSM 103164	contig	GCA_013408305	1347891.3	2834019907	1347891	75.79

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
42950	Naumannella cuiyingiana strain AFT2 16S ribosomal RNA gene, partial sequence	KF041479	1484		1347891

GC content

@ref	GC-content (mol%)	Method
43844	71.97	high performance liquid chromatography (HPLC)
42950	71.97

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1340830v1 assembly for Naumannella cuiyingiana DSM 103164
NCBI: GCA_013408305

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.37	no
125439	motility	BacteriaNetⓘ	no	64.23	no
125439	gram_stain	BacteriaNetⓘ	positive	84.75	no
125439	spore_formation	BacteriaNetⓘ	no	83.34	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Naumannella cuiyingiana strain DSM 103164
PATRIC: 1347891.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	91.37	yes
125438	anaerobic	anaerobicⓘ	no	97.70	yes
125438	spore-forming	spore-formingⓘ	no	69.69	yes
125438	aerobic	aerobicⓘ	yes	90.04	yes
125438	thermophilic	thermophileⓘ	no	96.00	yes
125438	flagellated	motile2+ⓘ	no	94.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Description of Naumannella cuiyingiana sp. nov., isolated from a ca. 1500-year-old mural painting, and emended description of the genus Naumannella.	Tian T, Wu F, Ma Y, Xiang T, Ma W, Hu W, Wu G, An L, Wang W, Feng H	Int J Syst Evol Microbiol	10.1099/ijsem.0.001964	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#42950	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103164
#43844	Tian Tian, Fasi Wu, Yantian Ma, Ting Xiang, Wenxia Ma, Weigang Hu, Guangwen Wu, Lizhe An, Wanfu Wang, Huyuan Feng: Description of Naumannella cuiyingiana sp. nov., isolated from a ca. 1500-year-old mural painting, and emended description of the genus Naumannella. IJSEM 67: 2609 - 2614 2017 ( DOI 10.1099/ijsem.0.001964 , PubMed 28792371 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Naumannella cuiyingiana (DSM 103164, CCTCC AB 2015428, AFT2)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Enzymes

Fatty acid profile

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_013408305

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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