Name: Naumannella cuiyingiana AFT2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140343

Type strain:

Species: Naumannella cuiyingiana

Strain Designation: AFT2

Culture col. no.: DSM 103164, CCTCC AB 2015428, JCM 33579

Strain history: CCTCC AB 2015428 <-- H. Feng; Sch. of Life Sci., Lanzhou Univ., China; AFT2.

NCBI tax ID(s): 1347891 (species)

SI-ID 401338

DSM 103164

Section

Naumannella cuiyingiana AFT2 is an aerobe, Gram-positive, oval-shaped bacterium that forms circular colonies and was isolated from mural painting of a 1500-year-tomb.

colony-forming
Gram-positive
oval-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Propionibacteriales*
	Family	*Propionibacteriaceae*
	Genus	*Naumannella*
	Species	**Naumannella cuiyingiana**
	Full Scientific Name (LPSN)	Naumannella cuiyingiana Tian et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43844]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #43844]	Cell length	0.6-1.0 µm

[Ref.: #43844]	Cell width	0.3-0.7 µm

[Ref.: #43844]	Cell shape	oval-shaped

[Ref.: #43844]	Motility	no

[Ref.: #43844]	Colony size	2 mm
[Ref.: #43844]	Colony color	white
[Ref.: #43844]	Colony shape	circular
[Ref.: #43844]	Incubation period	3 days
[Ref.: #43844]	Cultivation medium used	Tryptic soy agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43844]

Culture medium

Tryptic soy agar

[Ref.: #43844]

Culture medium growth

[Ref.: #42950]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #42950]

Culture medium growth

[Ref.: #42950]

Culture medium link

Medium recipe at MediaDive

[Ref.: #42950]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #42950]	growth	28 °C
[Ref.: #43844]	growth	20-37 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #42950]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43844]			growth	6.0-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43844]

Oxygen tolerance

aerobe

[Ref.: #43844]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	yes Confidence in %90
[Ref.: #69480]	Ability of spore formation	no Confidence in %98.808

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43844]		NaCl		growth	0-12	%(w/v)

[Ref.: #43844]	Observation	quinone MK-9
[Ref.: #67770]	Observation	quinones: MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43844]	2,3-butanediol ChEBI	-	carbon source
[Ref.: #43844]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #43844]	2-deoxyadenosine ChEBI	+	carbon source
[Ref.: #43844]	2-oxoglutarate ChEBI	-	carbon source
[Ref.: #43844]	3-O-methyl-D-glucose ChEBI	+	carbon source
[Ref.: #43844]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #43844]	adenosine ChEBI	-	carbon source
[Ref.: #43844]	adipate ChEBI	-	assimilation
[Ref.: #43844]	alpha-cyclodextrin ChEBI	-	carbon source
[Ref.: #43844]	alpha-D-glucose ChEBI	-	carbon source
[Ref.: #43844]	amp ChEBI	-	carbon source
[Ref.: #43844]	amygdalin ChEBI	-	builds acid from
[Ref.: #43844]	amygdalin ChEBI	-	carbon source
[Ref.: #43844]	arabinose ChEBI	-	assimilation
[Ref.: #43844]	arbutin ChEBI	-	builds acid from
[Ref.: #43844]	arbutin ChEBI	+	carbon source
[Ref.: #43844]	beta-cyclodextrin ChEBI	+	carbon source
[Ref.: #43844]	casein	-	hydrolysis
[Ref.: #43844]	cellobiose ChEBI	-	builds acid from
[Ref.: #43844]	citrate ChEBI	-	assimilation
[Ref.: #43844]	D-alanine ChEBI	+	carbon source
[Ref.: #43844]	D-arabinose ChEBI	+	builds acid from
[Ref.: #43844]	D-arabinose ChEBI	+	carbon source
[Ref.: #43844]	D-arabitol ChEBI	-	builds acid from
[Ref.: #43844]	D-fructose ChEBI	-	builds acid from
[Ref.: #43844]	D-fructose ChEBI	+	carbon source
[Ref.: #43844]	D-fructose 6-phosphate ChEBI	+	carbon source
[Ref.: #43844]	D-galactose ChEBI	-	builds acid from
[Ref.: #43844]	D-galactose ChEBI	+	carbon source
[Ref.: #43844]	D-galacturonic acid ChEBI	+	carbon source
[Ref.: #43844]	D-gluconate ChEBI	+	carbon source
[Ref.: #43844]	D-glucose ChEBI	-	builds acid from
[Ref.: #43844]	D-glucose ChEBI	+	carbon source
[Ref.: #43844]	D-glucose 6-phosphate ChEBI	+	carbon source
[Ref.: #43844]	D-lyxose ChEBI	-	builds acid from
[Ref.: #43844]	D-mannitol ChEBI	-	builds acid from
[Ref.: #43844]	D-mannitol ChEBI	+	carbon source
[Ref.: #43844]	D-mannose ChEBI	-	builds acid from
[Ref.: #43844]	D-mannose ChEBI	+	carbon source
[Ref.: #43844]	D-psicose ChEBI	+	carbon source
[Ref.: #43844]	D-ribose ChEBI	-	builds acid from
[Ref.: #43844]	D-ribose ChEBI	+	carbon source
[Ref.: #43844]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #43844]	D-sorbitol ChEBI	+	carbon source
[Ref.: #43844]	D-tagatose ChEBI	-	builds acid from
[Ref.: #43844]	D-xylose ChEBI	-	builds acid from
[Ref.: #43844]	decanoate ChEBI	-	assimilation
[Ref.: #43844]	dextrin ChEBI	-	carbon source
[Ref.: #43844]	erythritol ChEBI	-	builds acid from
[Ref.: #43844]	esculin ChEBI	+	builds acid from
[Ref.: #43844]	esculin ChEBI	+	hydrolysis
[Ref.: #43844]	galactitol ChEBI	-	builds acid from
[Ref.: #43844]	gelatin ChEBI	+	hydrolysis
[Ref.: #43844]	gentiobiose ChEBI	+	carbon source
[Ref.: #43844]	gluconate ChEBI	-	builds acid from
[Ref.: #43844]	gluconate ChEBI	+/-	assimilation
[Ref.: #43844]	glucose ChEBI	+	assimilation
[Ref.: #43844]	glucose 1-phosphate ChEBI	-	carbon source
[Ref.: #43844]	glycerol ChEBI	-	builds acid from
[Ref.: #43844]	glycerol ChEBI	-	carbon source
[Ref.: #43844]	glycerol 1-phosphate ChEBI	-	carbon source
[Ref.: #43844]	glycogen ChEBI	-	builds acid from
[Ref.: #43844]	glycogen ChEBI	-	carbon source
[Ref.: #43844]	glycyl-l-glutamate ChEBI	+	carbon source
[Ref.: #43844]	inosine ChEBI	-	carbon source
[Ref.: #43844]	inulin ChEBI	-	builds acid from
[Ref.: #43844]	inulin ChEBI	+	carbon source
[Ref.: #43844]	L-alanine ChEBI	-	carbon source
[Ref.: #43844]	L-alanylglycine ChEBI	-	carbon source
[Ref.: #43844]	L-arabinose ChEBI	-	builds acid from
[Ref.: #43844]	L-arabinose ChEBI	+	carbon source
[Ref.: #43844]	L-arabitol ChEBI	-	builds acid from
[Ref.: #43844]	L-aspartate ChEBI	+	carbon source
[Ref.: #43844]	L-glutamate ChEBI	+	carbon source
[Ref.: #43844]	L-pyroglutamic acid ChEBI	-	carbon source
[Ref.: #43844]	L-rhamnose ChEBI	+	builds acid from
[Ref.: #43844]	L-rhamnose ChEBI	+	carbon source
[Ref.: #43844]	L-serine ChEBI	+	carbon source
[Ref.: #43844]	L-xylose ChEBI	-	builds acid from
[Ref.: #43844]	lactamide ChEBI	-	carbon source
[Ref.: #43844]	lactate ChEBI	-	builds acid from
[Ref.: #43844]	lactate ChEBI	+	carbon source
[Ref.: #43844]	lactulose ChEBI	+	carbon source
[Ref.: #43844]	malate ChEBI	+	assimilation
[Ref.: #43844]	maltose ChEBI	-	builds acid from
[Ref.: #43844]	maltose ChEBI	+	assimilation
[Ref.: #43844]	maltose ChEBI	+	carbon source
[Ref.: #43844]	maltotriose ChEBI	+	carbon source
[Ref.: #43844]	mannan ChEBI	-	carbon source
[Ref.: #43844]	mannitol ChEBI	+	assimilation
[Ref.: #43844]	mannose ChEBI	+	assimilation
[Ref.: #43844]	melezitose ChEBI	-	builds acid from
[Ref.: #43844]	melezitose ChEBI	+	carbon source
[Ref.: #43844]	melibiose ChEBI	-	builds acid from
[Ref.: #43844]	melibiose ChEBI	+	carbon source
[Ref.: #43844]	methyl alpha-D-galactoside ChEBI	+	carbon source
[Ref.: #43844]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #43844]	methyl alpha-D-glucopyranoside ChEBI	+	carbon source
[Ref.: #43844]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #43844]	methyl alpha-D-mannoside ChEBI	+	carbon source
[Ref.: #43844]	methyl beta-D-galactoside ChEBI	+	carbon source
[Ref.: #43844]	methyl beta-D-glucopyranoside ChEBI	-	carbon source
[Ref.: #43844]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #43844]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43844]	myo-inositol ChEBI	-	carbon source
[Ref.: #43844]	N-acetyl-L-glutamate ChEBI	-	carbon source
[Ref.: #43844]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #43844]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #43844]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #43844]	nitrate ChEBI	-	reduction
[Ref.: #43844]	phenylacetate ChEBI	-	assimilation
[Ref.: #43844]	putrescine ChEBI	+	carbon source
[Ref.: #43844]	pyruvate ChEBI	-	carbon source
[Ref.: #43844]	raffinose ChEBI	-	builds acid from
[Ref.: #43844]	raffinose ChEBI	+	carbon source
[Ref.: #43844]	ribitol ChEBI	-	builds acid from
[Ref.: #43844]	salicin ChEBI	-	builds acid from
[Ref.: #43844]	salicin ChEBI	+	carbon source
[Ref.: #43844]	sorbose ChEBI	-	builds acid from
[Ref.: #43844]	stachyose ChEBI	+	carbon source
[Ref.: #43844]	starch ChEBI	-	builds acid from
[Ref.: #43844]	starch ChEBI	+	hydrolysis
[Ref.: #43844]	sucrose ChEBI	-	builds acid from
[Ref.: #43844]	sucrose ChEBI	-	carbon source
[Ref.: #43844]	thymidine ChEBI	-	carbon source
[Ref.: #43844]	thymidine 5'-monophosphate ChEBI	-	carbon source
[Ref.: #43844]	trehalose ChEBI	-	builds acid from
[Ref.: #43844]	trehalose ChEBI	+	carbon source
[Ref.: #43844]	turanose ChEBI	-	builds acid from
[Ref.: #43844]	tween 40 ChEBI	+	carbon source
[Ref.: #43844]	tween 80 ChEBI	+	carbon source
[Ref.: #43844]	uridine ChEBI	-	carbon source
[Ref.: #43844]	uridine 5'-monophosphate ChEBI	+	carbon source
[Ref.: #43844]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43844]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43844]	acid phosphatase	+/-	3.1.3.2
[Ref.: #43844]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43844]	alpha-galactosidase	+	3.2.1.22
[Ref.: #43844]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43844]	alpha-mannosidase	+	3.2.1.24
[Ref.: #43844]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43844]	beta-D-fucosidase	-	3.2.1.38
[Ref.: #43844]	beta-galactosidase	+	3.2.1.23
[Ref.: #43844]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43844]	catalase	+	1.11.1.6
[Ref.: #43844]	chymotrypsin	-	3.4.4.5
[Ref.: #43844]	cystine arylamidase	+	3.4.11.3
[Ref.: #43844]	esterase (C 4)	+
[Ref.: #43844]	esterase Lipase (C 8)	+
[Ref.: #43844]	leucine arylamidase	+	3.4.11.1
[Ref.: #43844]	lipase (C 14)	-
[Ref.: #43844]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43844]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43844]	trypsin	-	3.4.21.4
[Ref.: #43844]	urease	-	3.5.1.5
[Ref.: #43844]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43844]

Fatty acid	Percentage
C15:0 anteiso	55.32
C17:0 anteiso	9.33
C15:0 iso	6.55
C16:0 iso	5.12
C16:0	4.37
C16:1ω7c / C16:1ω6c	3.22
C18:1ω9c	3.18
C18:0	2.04
C17:0	1.6
C18:1ω7c / C18:1ω6c	1.29
C17:0 iso	1.27
C17:0 iso 3OH	1.03

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #42950]	Sample type/isolated from	mural painting of a 1500-year-tomb
[Ref.: #42950]	Geographic location (country and/or sea, region)	Xianxiu Xu's grave, Taiyuan
[Ref.: #42950]	Country	China
[Ref.: #42950]	Country ISO 3 Code	CHN
[Ref.: #42950]	Continent	Asia
[Ref.: #42950]	Geographic location	37.8366°/112.6120°

[Ref.: #43844]	Sample type/isolated from	mural painting sample from ca. 1500-year-old tomb located in the Northern Qi Dynasty in Shanxi Province, China
[Ref.: #43844]	Geographic location (country and/or sea, region)	Shanxi Province
[Ref.: #43844]	Country	China
[Ref.: #43844]	Country ISO 3 Code	CHN
[Ref.: #43844]	Continent	Asia
[Ref.: #43844]	Enrichment culture	PYGV liquid medium
[Ref.: #43844]	Enrichment culture composition	Peptone (0.025%, w/v), yeast extract (0.025%, w/v), glucose (0.025%, w/v), 20 ml per litre of mineral salt solution [10 g/L nitrilotriacetic acid, 29.7 g/L MgSO4.7H2O, 3.34 g/L CaCl2.2H2O, 12.67 mg/L Na2MoO4.2H2O, 99 mg/L FeSO4.7H2O and 50 ml metal salt solution (250 mg sodium EDTA, 1095 mg ZnSO4.7H2O, 500 mg/L FeSO4.7H2O, 154 mg/L MnSO4.H2O, 39.2 mg/L CuSO4.5H2O, 24.8 mg/L Co(NO3)2.6H2O and 17.7 mg/L Na2B4O7.10H2O), pH 7.2] and 5 ml per litre of vitamin solution (4 mg/L biotin, 4 mg/L
[Ref.: #43844]	Enrichment culture duration	15 days
[Ref.: #43844]	Enrichment culture temperature	20 ̊C

[Ref.: #67770]	Sample type/isolated from	Mural painting sample from ca. 1500-year-old tomb
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered

#Other

#Painting

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KF041479 (>99% sequence identity) for Naumannella from Microbeatlas

Aquatic Samples	264
Soil Samples	288
Animal Samples	744
Plant Samples	42
Total Samples	1338

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #42950]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #42950]	Naumannella cuiyingiana strain AFT2 16S ribosomal RNA gene, partial sequence	KF041479	1484	ENA	1347891 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Naumannella cuiyingiana DSM 103164	GCA_013408305	contig	GenBank	1347891 tax ID
[Ref.: #66792]	Naumannella cuiyingiana strain DSM 103164	1347891.3	wgs	PATRIC	1347891 tax ID	*
[Ref.: #66792]	Naumannella cuiyingiana DSM 103164	2834019907	draft	JGI IMG/M	1347891 tax ID	*

[Ref.: #43844]	GC-content	71.97 mol%	high performance liquid chromatography (HPLC)
[Ref.: #42950]	GC-content	71.97 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Naumannella cuiyingiana strain DSM 103164 PATRIC: 1347891.3
[Ref.: #69481]	spore-forming	yes	90	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Naumannella cuiyingiana strain DSM 103164 PATRIC: 1347891.3
[Ref.: #69480]	motile	no	94.39	no
[Ref.: #69480]	gram-positive	yes	87.951	yes
[Ref.: #69480]	anaerobic	no	99.45	no
[Ref.: #69480]	halophile	no	80.758	no
[Ref.: #69480]	spore-forming	no	85.898	yes
[Ref.: #69480]	thermophile	no	97.307	yes
[Ref.: #69480]	glucose-util	yes	84.02	no
[Ref.: #69480]	aerobic	yes	93.244	yes
[Ref.: #69480]	flagellated	no	98.458	no
[Ref.: #69480]	glucose-ferment	no	85.811	no

Availability in culture collections External links

[Ref.: #42950]

Culture collection no.

DSM 103164, CCTCC AB 2015428, JCM 33579

[Ref.: #96642]

SI-ID 401338

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Description of Naumannella cuiyingiana sp. nov., isolated from a ca. 1500-year-old mural painting, and emended description of the genus Naumannella.	Tian T, Wu F, Ma Y, Xiang T, Ma W, Hu W, Wu G, An L, Wang W, Feng H	Int J Syst Evol Microbiol	10.1099/ijsem.0.001964	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#42950

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103164

#43844

Tian Tian, Fasi Wu, Yantian Ma, Ting Xiang, Wenxia Ma, Weigang Hu, Guangwen Wu, Lizhe An, Wanfu Wang, Huyuan Feng: Description of Naumannella cuiyingiana sp. nov., isolated from a ca. 1500-year-old mural painting, and emended description of the genus Naumannella. IJSEM 67: 2609 - 2614 2017 ( DOI 10.1099/ijsem.0.001964 , PubMed 28792371 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#96642

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401338.1 )

* These data were automatically processed and therefore are not curated

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Naumannella cuiyingiana strain DSM 103164

Naumannella cuiyingiana strain DSM 103164

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