General

@ref: 42735

BacDive-ID: 140128

DSM-Number: 26261

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Sphaerochaeta associata GLS2 is a mesophilic bacterium that was isolated from culture of Methanosarcina mazei JL01.

NCBI tax id

• NCBI tax id: 1129264
• Matching level: species

strain history

• @ref: 42735
• history: <- V. Shcherbakova, Russian Academy of Sciences, Inst. of Biochem. and Physiol. of Microorganisms, Moscow region; GLS2 <- V. Shcherbakova and O.Y. Troshina {2011}

doi: 10.13145/bacdive140128.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/spirochaetota
• domain: Bacteria
• phylum: Spirochaetota
• class: Spirochaetia
• order: Spirochaetales
• family: Sphaerochaetaceae
• genus: Sphaerochaeta
• species: Sphaerochaeta associata
• full scientific name: Sphaerochaeta associata Troshina et al. 2015

@ref: 42735

domain: Bacteria

phylum: Spirochaetes

class: Spirochaetia

order: Spirochaetales

family: Sphaerochaetaceae

genus: Sphaerochaeta

species: Sphaerochaeta associata

full scientific name: Sphaerochaeta associata Troshina et al. 2015

strain designation: GLS2

type strain: yes

Culture and growth conditions

culture medium

• @ref: 42735
• name: 1/2 TRYPTIC SOY BROTH (ANAEROBE) (DSMZ Medium 1205)
• growth: yes
• link: https://mediadive.dsmz.de/medium/1205
• composition: Name: 1/2 TRYPTIC SOY BROTH (ANAEROBIC) (DSMZ Medium 1205) Composition: Trypticase soy broth 13.75 g/l D-Glucose 3.6 g/l NaCl 2.5 g/l Yeast extract 1.0 g/l DL-Dithiothreitol 0.15 g/l Distilled water

culture temp

• @ref: 42735
• growth: positive
• type: growth
• temperature: 37
• range: mesophilic

Isolation, sampling and environmental information

isolation

• @ref: 42735
• sample type: culture of Methanosarcina mazei JL01
• host species: Methanosarcina mazei
• country: Russia
• origin.country: RUS
• continent: Europe

isolation source categories

taxonmaps

• @ref: 69479
• File name: preview.99_36478.png
• url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_431;96_1992;97_2394;98_2957;99_36478&stattab=map
• Last taxonomy: Sphaerochaeta associata
• 16S sequence: JN944166
• Sequence Identity:
• Total samples: 1333
• soil counts: 96
• aquatic counts: 1050
• animal counts: 102
• plant counts: 85

Safety information

risk assessment

• @ref: 42735
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 42735
• description: Sphaerochaeta associata strain GLS2 16S ribosomal RNA gene, partial sequence
• accession: JN944166
• length: 1504
• database: ena
• NCBI tax ID: 1129264

Genome sequences

• @ref: 66792
• description: Sphaerochaeta associata GLS2
• accession: 2706794916
• assembly level: draft
• database: img
• NCBI tax ID: 1129264

GC content

• @ref: 42735
• GC-content: 47.2
• method: high performance liquid chromatography (HPLC)

External links

@ref: 42735

culture collection no.: DSM 26261, VKM B-2742

straininfo link

• @ref: 96522
• straininfo: 401415

literature

• topic: Phylogeny
• Pubmed-ID: 26341497
• title: Sphaerochaeta associata sp. nov., a spherical spirochaete isolated from cultures of Methanosarcina mazei JL01.
• authors: Troshina O, Oshurkova V, Suzina N, Machulin A, Ariskina E, Vinokurova N, Kopitsyn D, Novikov A, Shcherbakova V
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijsem.0.000575
• year: 2015
• mesh: Arctic Regions, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Methanosarcina, Molecular Sequence Data, Nucleic Acid Hybridization, Permafrost/*microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spirochaetaceae/*classification/genetics/isolation & purification
• topic2: Genetics

Reference