Workshop and Q&A October 6th

Strain identifier

BacDive ID: 1400

Type strain: Yes

Species: Ureibacillus composti

Strain Designation: HC145

Strain history: <- Soon-Wo Kwon, HC145

NCBI tax ID(s): 367742 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 7285

BacDive-ID: 1400

DSM-Number: 17951

keywords: rod-shaped, motile, Gram-negative, thermophilic, spore-forming, Bacteria, 16S sequence, colony-forming

description: Ureibacillus composti HC145 is a spore-forming, thermophilic, Gram-negative bacterium that forms circular colonies and was isolated from livestock-manure compost.

NCBI tax id

  • NCBI tax id: 367742
  • Matching level: species

strain history: <- Soon-Wo Kwon, HC145

doi: 10.13145/bacdive1400.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Caryophanaceae
  • genus: Ureibacillus
  • species: Ureibacillus composti
  • full scientific name: Ureibacillus composti Weon et al. 2007

@ref: 7285

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Caryophanaceae

genus: Ureibacillus

species: Ureibacillus composti

full scientific name: Ureibacillus composti Weon et al. 2007

strain designation: HC145

type strain: yes

Morphology

cell morphology

  • @ref: 23026
  • gram stain: negative
  • cell length: 2.5-4.0 µm
  • cell width: 0.7-0.9 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

@refcolony colorcolony shapemedium used
23026light-browncirculartrypticase soy agar
23026nutrient agar (Oxoid)

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7285CASO AGAR (MERCK 105458) (DSMZ Medium 220)yeshttps://bacmedia.dsmz.de/medium/220Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
23026nutrient agar (Oxoid)yes
23026trypticase soy agaryes

culture temp

@refgrowthtypetemperaturerange
7285positivegrowth50thermophilic
23026positivegrowth37.0-60.0
23026nogrowth35.0mesophilic
23026nogrowth65.0thermophilic
23026thermophilic

culture pH

  • @ref: 23026
  • ability: positive
  • type: growth
  • pH: 6.0-8.0

Physiology and metabolism

spore formation

  • @ref: 23026
  • spore description: spherical, subterminal or terminal
  • type of spore: endospore
  • spore formation: yes

halophily

  • @ref: 23026
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: <5.0 %

murein

@refmurein short keytype
7285A11.31A4alpha L-Lys-D-Asp
23026A11.31A4alpha L-Lys-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2302617634D-glucose-builds acid from
2302616899D-mannitol-builds acid from
2302665327D-xylose-builds acid from
2302630849L-arabinose-builds acid from
2302617234glucose-fermentation
23026casein-hydrolysis
230265291gelatin-hydrolysis
2302628017starch-hydrolysis
2302618186tyrosine-hydrolysis
2302616199urea-hydrolysis
2302617632nitrate-reduction
230264853esculin+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
2302635581indoleno
2302615688acetoinno

metabolite tests

  • @ref: 23026
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
23026catalase+1.11.1.6
23026cytochrome oxidase+1.9.3.1
23026phenylalanine deaminase+4.3.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture temperature
7285livestock-manure compostIchonRepublic of KoreaKORAsia
23026livestock-manure composttrypticase soy agar55.0

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Livestock (Husbandry)
#Engineered#Biodegradation#Composting
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

  • @ref: 7285
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7285
  • description: Ureibacillus composti strain HC145 16S ribosomal RNA gene, partial sequence
  • accession: DQ348071
  • length: 1515
  • database: ena
  • NCBI tax ID: 367742

GC content

@refGC-contentmethod
728542.4
2302642.4high performance liquid chromatography (HPLC)

External links

@ref: 7285

culture collection no.: DSM 17951, KACC 11361

straininfo link

@refpassport
20218http://www.straininfo.net/strains/764581
20218http://www.straininfo.net/strains/764582

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18048747Ureibacillus composti sp. nov. and Ureibacillus thermophilus sp. nov., isolated from livestock-manure composts.Weon HY, Lee SY, Kim BY, Noh HJ, Schumann P, Kim JS, Kwon SWInt J Syst Evol Microbiol10.1099/ijs.0.65232-02007Amino Acids/analysis, Animals, Animals, Domestic, Bacillaceae/chemistry/*classification/genetics/*isolation & purification, Base Composition, Cell Wall/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Genes, rRNA, Korea, Manure/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Vitamin K 2/analysisGenetics
Phylogeny24491829Ureibacillus defluvii sp. nov., isolated from a thermophilic microbial fuel cell.Zhou S, Tang J, Qin D, Lu Q, Yang GInt J Syst Evol Microbiol10.1099/ijs.0.056655-02014Bacterial Typing Techniques, Base Composition, Bioelectric Energy Sources/*microbiology, Biofilms, DNA, Bacterial/genetics, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, Planococcaceae/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7285Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17951)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17951
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23026Hang-Yeon Weon,Seon-Young Lee,Byung-Yong Kim,Hyung-Jun Noh,Peter Schumann,Jong-Shik Kim,Soon-Wo Kwon10.1099/ijs.0.65232-0Ureibacillus composti sp. nov. and Ureibacillus thermophilus sp. nov., isolated from livestock-manure compostsIJSEM 57: 2908-2911 200718048747