Strain identifier

BacDive ID: 138864

Type strain: No

Species: Pseudomonas aeruginosa

Strain Designation: 958/1953-221

Strain history: CIP <- 1959, H. Kleinmaier, Hyg. Inst., Bonn, Germany: strain 958/1953-221

NCBI tax ID(s): 287 (species)

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General

@ref: 40835

BacDive-ID: 138864

keywords: genome sequence, Bacteria, obligate aerobe, Gram-negative

description: Pseudomonas aeruginosa 958/1953-221 is an obligate aerobe, Gram-negative bacterium that was isolated from Unknown source.

NCBI tax id

  • NCBI tax id: 287
  • Matching level: species

strain history

  • @ref: 40835
  • history: CIP <- 1959, H. Kleinmaier, Hyg. Inst., Bonn, Germany: strain 958/1953-221

doi: 10.13145/bacdive138864.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas aeruginosa
  • full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacterium aeruginosum

@ref: 40835

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas aeruginosa

strain designation: 958/1953-221

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes91.15
6948097.5negative
40835yesnegativerod-shaped

colony morphology

  • @ref: 40835

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
40835MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
40835CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
40835CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

  • @ref: 40835
  • growth: positive
  • type: growth
  • temperature: 30

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
69480aerobe95.872
40835obligate aerobe

spore formation

  • @ref: 69481
  • spore formation: no
  • confidence: 100

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4083517632nitrate+reduction
4083516301nitrite+reduction

metabolite production

  • @ref: 40835
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
40835oxidase+
40835catalase+1.11.1.6
40835urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
40835-+++++--+-++--------

Isolation, sampling and environmental information

isolation

  • @ref: 40835
  • sample type: Unknown source

Safety information

risk assessment

  • @ref: 40835
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudomonas aeruginosa ATCC 33348GCA_001420225scaffoldncbi287
66792Pseudomonas aeruginosa NCTC11440GCA_900706995contigncbi287
66792Pseudomonas aeruginosa strain ATCC 33348287.2002wgspatric287
66792Pseudomonas aeruginosa strain NCTC11440287.9431wgspatric287
66792Pseudomonas aeruginosa ATCC 333482671180317draftimg287

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno100no
69480gram-positivegram-positivePositive reaction to Gram-stainingno97.5no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no97.86yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes95.872no
69480spore-formingspore-formingAbility to form endo- or exosporesno85.028no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno99no
69480flagellatedmotile2+Ability to perform flagellated movementyes91.15no

External links

@ref: 40835

culture collection no.: CIP 59.33, ATCC 33348, NCTC 11440

straininfo link

  • @ref: 95500
  • straininfo: 7940

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
40835Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2059.33Collection of Institut Pasteur (CIP 59.33)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
95500Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID7940.1