Strain identifier

BacDive ID: 138198

Type strain: Yes

Species: Actinobacillus minor

Strain Designation: NM305, NM 305

Strain history: CIP <- 1997, K. Moeller, Danish Vet. Lab., Copenhagen, Denmark: strain NM 305 <- K. Moeller <- S. Rosendal

NCBI tax ID(s): 51047 (species)

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General

@ref: 39258

BacDive-ID: 138198

DSM-Number: 113419

keywords: genome sequence, Bacteria, facultative anaerobe, mesophilic, Gram-negative, rod-shaped

description: Actinobacillus minor NM305 is a facultative anaerobe, mesophilic, Gram-negative bacterium of the family Pasteurellaceae.

NCBI tax id

  • NCBI tax id: 51047
  • Matching level: species

strain history

@refhistory
68861<- CIP; CIP 105314 <- K. Moeller, Danish Vet. Lab.; NM305 <- S. Rosendal;
39258CIP <- 1997, K. Moeller, Danish Vet. Lab., Copenhagen, Denmark: strain NM 305 <- K. Moeller <- S. Rosendal

doi: 10.13145/bacdive138198.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Actinobacillus
  • species: Actinobacillus minor
  • full scientific name: Actinobacillus minor Møller et al. 1996

@ref: 39258

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Actinobacillus

species: Actinobacillus minor

full scientific name: Actinobacillus minor Møller et al. 1996

strain designation: NM305, NM 305

type strain: yes

Morphology

cell morphology

  • @ref: 39258
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
68861HAEMOPHILUS MEDIUM (DSMZ Medium 804)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium804.pdf
39258MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and NeisseriayesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
39258CIP Medium 10yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10

culture temp

@refgrowthtypetemperaturerange
39258positivegrowth37mesophilic
68861positivegrowth37mesophilic
39258positivegrowth25-41
39258nogrowth15psychrophilic
39258nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 39258
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
39258esculin-hydrolysis4853
39258hippurate-hydrolysis606565
39258nitrate+reduction17632
39258nitrite+reduction16301
68377D-glucose-builds acid from17634
68377D-fructose-builds acid from15824
68377maltose-builds acid from17306
68377sucrose-builds acid from17992
68377ornithine-degradation18257
68377urea+hydrolysis16199
68377tryptophan-energy source27897

antibiotic resistance

  • @ref: 39258
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
3925835581indoleno

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68377tryptophan deaminase-4.1.99.1
68377gamma-glutamyltransferase-2.3.2.2
68377proline-arylamidase-3.4.11.5
68377beta-galactosidase+3.2.1.23
68377alkaline phosphatase+3.1.3.1
68377lipase-
68377urease+3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68377beta-lactamase+3.5.2.6
39258oxidase-
39258beta-galactosidase+3.2.1.23
39258alcohol dehydrogenase-1.1.1.1
39258catalase-1.11.1.6
39258gamma-glutamyltransferase-2.3.2.2
39258lysine decarboxylase-4.1.1.18
39258ornithine decarboxylase-4.1.1.17
39258urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
39258-+--------++++------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
39258----+/-----++++--------+-----+++++--++/-+/---+--------+/-

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
39258+-----+-++---

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typelatitudelongitude
39258OntarioCanadaCANNorth America
54556GuelphCanadaCANNorth AmericaPig upper respiratory tract
68861GuelphCanadaCANNorth AmericaPig upper respiratory tract43.5459-80.256
39258OntarioCanadaCANNorth AmericaAnimal, Pig, respiratory tract

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Suidae (Pig,Swine)
#Host Body-Site#Oral cavity and airways#Airways

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
688611Risk group (German classification)
392581Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Actinobacillus minor NM305GCA_000175195contigncbi637911
66792Actinobacillus minor NM305637911.3wgspatric637911

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno97.878no
anaerobicno96.74no
halophileno55.992no
spore-formingno98.713no
glucose-utilno58.078no
thermophileno98.406yes
flagellatedno94.78no
aerobicno93.716no
motileno91.777no
glucose-fermentyes52.666no

External links

@ref: 39258

culture collection no.: CIP 105314, CCUG 38923, DSM 113419

straininfo link

  • @ref: 94975
  • straininfo: 51115

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny8863422Actinobacillus minor sp. nov., Actinobacillus porcinus sp. nov., and Actinobacillus indolicus sp. nov., three new V factor-dependent species from the respiratory tract of pigs.Moller K, Fussing V, Grimont PA, Paster BJ, Dewhirst FE, Kilian MInt J Syst Bacteriol10.1099/00207713-46-4-9511996Actinobacillus/*classification, Animals, Base Sequence, DNA, Bacterial/analysis, DNA, Ribosomal/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, RNA, Ribosomal, 16S/genetics, Respiratory System/*microbiology, Swine/*microbiologyGenetics
Metabolism19819087Production of haemolysins by strains of the Actinobacillus minor/"porcitonsillarum" complex.Arya G, Niven DFVet Microbiol10.1016/j.vetmic.2009.09.0302009Actinobacillus/*genetics/growth & development/*metabolism, Actinobacillus Infections/microbiology/veterinary, Animals, Bacterial Proteins/genetics/metabolism/pharmacology, Erythrocytes/drug effects, Genes, Bacterial/*genetics, Hemolysin Factors/genetics, Hemolysin Proteins/*genetics/*metabolism/pharmacology, Hot Temperature, Molecular Sequence Data, RNA, Ribosomal, 16S, Rabbits, Sequence Homology, Nucleic Acid, Sheep, Swine, Swine Diseases/microbiologyStress
Metabolism20888711Acquisition of haemoglobin-bound iron by strains of the Actinobacillus minor/"porcitonsillarum" complex.Arya G, Niven DFVet Microbiol10.1016/j.vetmic.2010.09.0042010Actinobacillus/genetics/growth & development/*metabolism, Animals, Bacterial Proteins/genetics, Carrier Proteins/genetics, Cattle, DNA, Bacterial/genetics, Hemoglobins/*chemistry, Humans, Iron/*metabolism, Siderophores/chemistry, Swine, Transferrin/*chemistry, Transferrin-Binding Proteins/geneticsPathogenicity
Genetics30363939Comparative Genomics of the First and Complete Genome of "Actinobacillus porcitonsillarum" Supports the Novel Species Hypothesis.Dona V, Perreten VInt J Genomics10.1155/2018/52617192018

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
39258Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105314Collection of Institut Pasteur (CIP 105314)
54556Curators of the CCUGhttps://www.ccug.se/strain?id=38923Culture Collection University of Gothenburg (CCUG) (CCUG 38923)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68371Automatically annotated from API 50CH acid
68377Automatically annotated from API NH
68382Automatically annotated from API zym
68861Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-113419Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 113419)
94975Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID51115.1