Name: Actinobacillus minor NM305
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 138198

Type strain:

Species: Actinobacillus minor

Strain Designation: NM305, NM 305

Culture col. no.: CIP 105314, CCUG 38923, DSM 113419

Strain history: CIP <- 1997, K. Moeller, Danish Vet. Lab., Copenhagen, Denmark: strain NM 305 <- K. Moeller <- S. Rosendal

NCBI tax ID(s): 51047 (species)

SI-ID 51115

CCUG 38923, CIP 105314, DSM 113419

Special Collections

Other strains from Actinobacillus minor

Section

When using BacDive for research please cite our paper

Actinobacillus minor NM305 is a facultative anaerobe, mesophilic, Gram-negative bacterium of the family Pasteurellaceae.

Gram-negative
rod-shaped
facultative anaerobe
mesophilic
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pasteurellales*
	Family	*Pasteurellaceae*
	Genus	*Actinobacillus*
	Species	**Actinobacillus minor**
	Full Scientific Name (LPSN)	Actinobacillus minor Møller et al. 1996

Information on morphological and physiological properties Morphology

[Ref.: #39258]	Gram stain	negative
[Ref.: #125438]	Gram stain	negative Confidence in %97.394

[Ref.: #39258]	Cell shape	rod-shaped

[Ref.: #39258]	Motility	no
[Ref.: #125438]	Motility	no Confidence in %94.06

Information on culture and growth conditions Culture and growth conditions

[Ref.: #68861]

Culture medium

HAEMOPHILUS MEDIUM (DSMZ Medium 804)

[Ref.: #68861]

Culture medium growth

[Ref.: #68861]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #39258]

Culture medium

MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and Neisseria

[Ref.: #39258]

Culture medium growth

[Ref.: #39258]

Culture medium composition

expand / minimize

[Ref.: #39258]

Culture medium

CIP Medium 10

[Ref.: #39258]

Culture medium growth

[Ref.: #39258]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #39258]		growth	37 °C
[Ref.: #39258]		growth	25-41 °C
[Ref.: #39258]	negative	growth	15 °C
[Ref.: #39258]	negative	growth	45 °C
[Ref.: #68861]		growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #39258]	Oxygen tolerance	facultative anaerobe
[Ref.: #125439]	Oxygen tolerance	obligate aerobe Confidence in %92.5

[Ref.: #125439]	Ability of spore formation	no Confidence in %94.3
[Ref.: #125438]	Ability of spore formation	no Confidence in %94.397

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68377]	D-fructose ChEBI	-	builds acid from	* from API NH
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68377]	D-glucose ChEBI	-	builds acid from	* from API NH
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #39258]	esculin ChEBI	-	hydrolysis
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #39258]	hippurate ChEBI	-	hydrolysis
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68377]	maltose ChEBI	-	builds acid from	* from API NH
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #39258]	nitrate ChEBI	+	reduction
[Ref.: #39258]	nitrite ChEBI	+	reduction
[Ref.: #68377]	ornithine ChEBI	-	degradation	* from API NH
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68377]	sucrose ChEBI	-	builds acid from	* from API NH
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68377]	tryptophan ChEBI	-	energy source	* from API NH
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68377]	urea ChEBI	+	hydrolysis	* from API NH
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #39258]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #39258]		indole ChEBI	no
[Ref.: #68377]		indole ChEBI	no	* from API NH

	Physiological tests	Metabolite	Indole test
[Ref.: #68377]		indole ChEBI	-	* from API NH

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #39258]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym, API NH
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #39258]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API NH
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68377]	beta-lactamase	+	3.5.2.6	* from API NH
[Ref.: #39258]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #39258]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #68377]	gamma-glutamyltransferase	-	2.3.2.2	* from API NH
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68377]	lipase	-		* from API NH
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #39258]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #39258]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68377]	ornithine decarboxylase	-	4.1.1.17	* from API NH
[Ref.: #39258]	oxidase	-
[Ref.: #68377]	proline-arylamidase	-	3.4.11.5	* from API NH
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68377]	tryptophan deaminase	-	4.1.99.1	* from API NH
[Ref.: #39258]	urease	+	3.5.1.5
[Ref.: #68377]	urease	+	3.5.1.5	* from API NH
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #39258]	10035	not determinedn.d.	-	-	-	-	+/-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	-	-	-	-	-	+	+	+	+	+	-	-	+	+/-	+/-	-	-	+	-	-	-	-	-	-	-	-	+/-

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #39258]	6926	+	-	-	-	-	-	+	-	+	+	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #39258]	8610	-	+	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #54556]	Sample type/isolated from	Pig upper respiratory tract
[Ref.: #54556]	Geographic location (country and/or sea, region)	Guelph
[Ref.: #54556]	Country	Canada
[Ref.: #54556]	Country ISO 3 Code	CAN
[Ref.: #54556]	Continent	North America

[Ref.: #68861]	Sample type/isolated from	Pig upper respiratory tract
[Ref.: #68861]	Geographic location (country and/or sea, region)	Guelph
[Ref.: #68861]	Country	Canada
[Ref.: #68861]	Country ISO 3 Code	CAN
[Ref.: #68861]	Continent	North America
[Ref.: #68861]	Geographic location	43.5459°/-80.2560°

[Ref.: #39258]	Sample type/isolated from	Animal, Pig, respiratory tract
[Ref.: #39258]	Geographic location (country and/or sea, region)	Ontario
[Ref.: #39258]	Country	Canada
[Ref.: #39258]	Country ISO 3 Code	CAN
[Ref.: #39258]	Continent	North America

[Ref.: #39258]	Geographic location (country and/or sea, region)	Ontario
[Ref.: #39258]	Country	Canada
[Ref.: #39258]	Country ISO 3 Code	CAN
[Ref.: #39258]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Suidae (Pig,Swine)
#Host Body-Site	#Oral cavity and airways	#Airways

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #68861]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #39258]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Actinobacillus minor NM305	GCA_000175195	contig	GenBank ENA	637911 tax ID	*
[Ref.: #66792]	Actinobacillus minor NM305	637911.3	wgs	PATRIC	637911 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinobacillus minor NM305 PATRIC: 637911.3
[Ref.: #125438]	gram-positive	gram-positiveⓘ	no	97.394	no
[Ref.: #125438]	anaerobic	anaerobicⓘ	no	91.807	yes
[Ref.: #125438]	aerobic	aerobicⓘ	no	84.778	yes
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	94.397	no
[Ref.: #125438]	thermophile	thermophilicⓘ	no	96.5	no
[Ref.: #125438]	motile2+	flagellatedⓘ	no	94.06	no

	Model	Trait	Prediction	Confidence in %
	Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinobacillus minor NM305 NCBI: GCA_000175195
[Ref.: #125439]	BacteriaNet	spore_formationⓘ	no	94.3
[Ref.: #125439]	BacteriaNet	motilityⓘ	no	71.1
[Ref.: #125439]	BacteriaNet	gram_stainⓘ	negative	82.5
[Ref.: #125439]	BacteriaNet	oxygen_toleranceⓘ	obligate aerobe	92.5

Availability in culture collections External links

[Ref.: #39258]

Culture collection no.

CIP 105314, CCUG 38923, DSM 113419

[Ref.: #94975]

SI-ID 51115

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Comparative Genomics of the First and Complete Genome of "Actinobacillus porcitonsillarum" Supports the Novel Species Hypothesis.	Dona V, Perreten V	Int J Genomics	10.1155/2018/5261719	2018	*
Metabolism	Acquisition of haemoglobin-bound iron by strains of the Actinobacillus minor/"porcitonsillarum" complex.	Arya G, Niven DF	Vet Microbiol	10.1016/j.vetmic.2010.09.004	2010	*
Metabolism	Production of haemolysins by strains of the Actinobacillus minor/"porcitonsillarum" complex.	Arya G, Niven DF	Vet Microbiol	10.1016/j.vetmic.2009.09.030	2009	*
Phylogeny	Actinobacillus minor sp. nov., Actinobacillus porcinus sp. nov., and Actinobacillus indolicus sp. nov., three new V factor-dependent species from the respiratory tract of pigs.	Moller K, Fussing V, Grimont PA, Paster BJ, Dewhirst FE, Kilian M	Int J Syst Bacteriol	10.1099/00207713-46-4-951	1996	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#39258 Collection of Institut Pasteur ; Curators of the CIP; CIP 105314
#54556 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 38923

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#68377

Automatically annotated from API NH .

#68382

Automatically annotated from API zym .

#68861

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 113419

#94975

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID51115.1 )

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#125439

Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .

* These data were automatically processed and therefore are not curated

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