Strain identifier

BacDive ID: 1380

Type strain: Yes

Species: Oceanobacillus neutriphilus

Strain Designation: A1g

Strain history: X.-W. Xu A1g.

NCBI tax ID(s): 531815 (species)

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General

@ref: 17756

BacDive-ID: 1380

DSM-Number: 24772

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Oceanobacillus neutriphilus A1g is an aerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from activated sludge of a bioreactor, Szechuan pickle factory.

NCBI tax id

  • NCBI tax id: 531815
  • Matching level: species

strain history

@refhistory
17756<- JCM <- X.-W. Xu, Second Inst. Oceanography, State Oceanic Admin., Hangzhou, China; strain A1g
67770X.-W. Xu A1g.

doi: 10.13145/bacdive1380.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Oceanobacillus
  • species: Oceanobacillus neutriphilus
  • full scientific name: Oceanobacillus neutriphilus Yang et al. 2010

@ref: 17756

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Oceanobacillus

species: Oceanobacillus neutriphilus

full scientific name: Oceanobacillus neutriphilus Yang et al. 2010

strain designation: A1g

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
23013positive1.5-2.5 µm0.7-1.2 µmrod-shapedyespolar
69480yes96.573
69480positive100

colony morphology

  • @ref: 23013
  • colony size: 1.0-2.0 mm
  • colony color: cream
  • colony shape: circular
  • incubation period: 2 days
  • medium used: MA medium

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17756BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
23013MA mediumyes
23013PY mediumyes

culture temp

@refgrowthtypetemperaturerange
17756positivegrowth30mesophilic
23013positivegrowth10.0-45.0
23013positiveoptimum37.0mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
23013positivegrowth6.0-9.0alkaliphile
23013positiveoptimum7.0
23013nogrowth<5.5
23013nogrowth>9.5

Physiology and metabolism

oxygen tolerance

  • @ref: 23013
  • oxygen tolerance: aerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
23013ellipsoidal,centralsporeyes
69481yes100
69480yes100

halophily

@refsaltgrowthtested relationconcentration
23013NaClpositivegrowth0.0-17.0 %(w/v)
23013NaCloptimum3.0-5.0 %

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2301316236ethanol-builds acid from
2301328066gentiobiose-builds acid from
2301330849L-arabinose-builds acid from
2301317266L-sorbose-builds acid from
2301317716lactose-builds acid from
2301317268myo-inositol-builds acid from
2301316634raffinose-builds acid from
2301330911sorbitol-builds acid from
23013casein-hydrolysis
2301316991dna-hydrolysis
230135291gelatin-hydrolysis
2301328017starch-hydrolysis
2301353426tween 80-hydrolysis
2301318186tyrosine-hydrolysis
2301316199urea-hydrolysis
2301317632nitrate-reduction
2301318305arbutin+builds acid from
2301317057cellobiose+builds acid from
2301315824D-fructose+builds acid from
2301317634D-glucose+builds acid from
2301316899D-mannitol+builds acid from
2301316024D-mannose+builds acid from
2301316443D-tagatose+builds acid from
230134853esculin+builds acid from
2301317754glycerol+builds acid from
2301317306maltose+builds acid from
23013506227N-acetylglucosamine+builds acid from
2301317814salicin+builds acid from
2301317992sucrose+builds acid from
2301327082trehalose+builds acid from
230134853esculin+hydrolysis
2301353423tween 40+hydrolysis
2301353425tween 60+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistantresistance conc.sensitivity conc.
230138309polymyxin byesnoyes300 Unit
2301317334penicillinyesnoyes10 µg (disc)
2301317076streptomycinyesnoyes10 µg (disc)
230137660nystatinyesnoyes100 µg (disc)
23013209807cefoxitinyesnoyes30 µg (disc)
2301327902tetracyclineyesnoyes30 µg (disc)
230132676amoxicillinyesyes10 µg (disc)
2301328971ampicillinyesyes10 µg (disc)
230133393carbenicillinyesyes100 µg (disc)
2301348923erythromycinyesyes15 µg (disc)
23013204928cefotaximeyesyes30 µg (disc)
2301317698chloramphenicolyesyes30 µg (disc)
230136104kanamycinyesyes30 µg (disc)
230137507neomycinyesyes30 µg (disc)
2301328368novobiocinyesyes30 µg (disc)
2301371415nitrofurantoinyesyes300 µg (disc)
2301328077rifampicinyesyes5 µg (disc)

metabolite production

  • @ref: 23013
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
23013acid phosphatase+3.1.3.2
23013alkaline phosphatase+3.1.3.1
23013alpha-chymotrypsin+3.4.21.1
23013alpha-fucosidase-3.2.1.51
23013alpha-galactosidase-3.2.1.22
23013alpha-glucosidase+3.2.1.20
23013alpha-mannosidase-3.2.1.24
23013arginine dihydrolase-3.5.3.6
23013beta-galactosidase+3.2.1.23
23013beta-galactosidase-3.2.1.23
23013beta-glucosidase+3.2.1.21
23013beta-glucuronidase-3.2.1.31
23013catalase+1.11.1.6
23013cystine arylamidase-3.4.11.3
23013cytochrome oxidase+1.9.3.1
23013esterase (C 4)+
23013esterase lipase (C 8)+
23013leucine arylamidase-3.4.11.1
23013lipase (C 14)-
23013lysine decarboxylase-4.1.1.18
23013N-acetyl-beta-glucosaminidase-3.2.1.52
23013naphthol-AS-BI-phosphohydrolase+
23013ornithine decarboxylase-4.1.1.17
23013trypsin-3.4.21.4
23013tryptophan deaminase-4.1.99.1
23013urease-3.5.1.5
23013valine arylamidase-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture temperature
17756activated sludge of a bioreactor, Szechuan pickle factoryChinaCHNAsia
23013activated sludge of a bioreactor treating salt-containing wastewater preserved Szechuan pickle factorysterile wastewater agar platescontaining per litre wastewater: 1.0 g glucose, 1.0 g peptone (BD) and 15.0 g agar (pH 7.0)30.0
67770Activated sludge of a bioreactor treating salt-containing wastewater

isolation source categories

Cat1Cat2Cat3
#Condition#Saline
#Engineered#Bioreactor
#Engineered#Industrial
#Engineered#Waste#Activated sludge

Safety information

risk assessment

  • @ref: 17756
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17756
  • description: Oceanobacillus neutriphilus strain A1g 16S ribosomal RNA gene, partial sequence
  • accession: EU709018
  • length: 1484
  • database: ena
  • NCBI tax ID: 531815

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Oceanobacillus neutriphilus CGMCC 1.7693GCA_014645515contigncbi531815
66792Oceanobacillus neutriphilus strain CGMCC 1.7693531815.3wgspatric531815
66792Oceanobacillus neutriphilus strain DSM 24772531815.4wgspatric531815
66792Oceanobacillus neutriphilus DSM 247722901131393draftimg531815

GC content

  • @ref: 17756
  • GC-content: 36.3
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveyes90.476no
anaerobicno97.977yes
halophileyes83.702no
spore-formingyes91.872yes
glucose-utilyes87.169no
thermophileno99.482no
motileyes88.157yes
aerobicyes92.968yes
flagellatedyes88.918yes
glucose-fermentno77.23no

External links

@ref: 17756

culture collection no.: DSM 24772, CGMCC 1.7693, JCM 15776, BCRC 80658, KCTC 13886

straininfo link

  • @ref: 71031
  • straininfo: 404977

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19946056Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor.Yang JY, Huo YY, Xu XW, Meng FX, Wu M, Wang CSInt J Syst Evol Microbiol10.1099/ijs.0.016295-02009Bacillaceae/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Bioreactors/*microbiology, Catalase/metabolism, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Esculin/metabolism, Fatty Acids/analysis, Hydrogen-Ion Concentration, Microscopy, Electron, Transmission, Molecular Sequence Data, Oxidoreductases/metabolism, Phylogeny, Pigments, Biological/biosynthesis, Polysorbates/metabolism, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Sodium Chloride/metabolismMetabolism
Phylogeny24595860Oceanobacillus aidingensis sp. nov., a moderately halophilic bacterium.Liu W, Yang SSAntonie Van Leeuwenhoek10.1007/s10482-014-0128-12014Amino Acids, Diamino/analysis, Bacillaceae/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Hydrogen-Ion Concentration, Lakes/microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Spores, Bacterial/cytology, TemperatureGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17756Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 24772)https://www.dsmz.de/collection/catalogue/details/culture/DSM-24772
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23013Jun-Yi Yang,Ying-Yi Huo,Xue-Wei Xu,Fan-Xu Meng,Min Wu,Chun-Sheng Wang10.1099/ijs.0.016295-0Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactorIJSEM 60: 2409-2414 201019946056
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71031Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID404977.1StrainInfo: A central database for resolving microbial strain identifiers