Name: Oceanobacillus neutriphilus A1g
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1380

Type strain:

Species: Oceanobacillus neutriphilus

Strain Designation: A1g

Culture col. no.: DSM 24772, CGMCC 1.7693, JCM 15776, BCRC 80658, KCTC 13886

Strain history: X.-W. Xu A1g.

NCBI tax ID(s): 531815 (species)

SI-ID 404977

DSM 24772

Special Collections

Section

Oceanobacillus neutriphilus A1g is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from activated sludge of a bioreactor, Szechuan pickle factory.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Oceanobacillus*
	Species	**Oceanobacillus neutriphilus**
	Full Scientific Name (LPSN)	Oceanobacillus neutriphilus Yang et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #23013]	Gram stain	positive

[Ref.: #23013]	Cell length	1.5-2.5 µm

[Ref.: #23013]	Cell width	0.7-1.2 µm

[Ref.: #23013]	Cell shape	rod-shaped

[Ref.: #23013]	Motility	yes

[Ref.: #23013]	Flagellum arrangement	polar

[Ref.: #23013]	Colony size	1.0-2.0 mm
[Ref.: #23013]	Colony color	cream
[Ref.: #23013]	Colony shape	circular
[Ref.: #23013]	Incubation period	2 days
[Ref.: #23013]	Cultivation medium used	MA medium

Information on culture and growth conditions Culture and growth conditions

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Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

MA medium

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Culture medium growth

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Culture medium

PY medium

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Culture medium growth

		Temperature
[Ref.: #17756]	growth	30 °C
[Ref.: #23013]	growth	10.0-45.0 °C
[Ref.: #23013]	optimum	37.0 °C
[Ref.: #67770]	growth	30 °C

	Kind of pH		pH
[Ref.: #23013]		optimum	7.0
[Ref.: #23013]		growth	6.0-9.0
[Ref.: #23013]	no	growth	<5.5
[Ref.: #23013]	no	growth	>9.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23013]

Oxygen tolerance

aerobe

[Ref.: #23013]	Spore description	ellipsoidal,central
[Ref.: #23013]	Type of spore	spore
[Ref.: #23013]	Ability of spore formation	yes
[Ref.: #69480]	Ability of spore formation	yes Confidence in %92.186
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23013]		NaCl		growth	0.0-17.0	%(w/v)
[Ref.: #23013]		NaCl		optimum	3.0-5.0	%

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23013]	arbutin ChEBI	+	builds acid from
[Ref.: #23013]	casein	-	hydrolysis
[Ref.: #23013]	cellobiose ChEBI	+	builds acid from
[Ref.: #23013]	D-fructose ChEBI	+	builds acid from
[Ref.: #23013]	D-glucose ChEBI	+	builds acid from
[Ref.: #23013]	D-mannitol ChEBI	+	builds acid from
[Ref.: #23013]	D-mannose ChEBI	+	builds acid from
[Ref.: #23013]	D-tagatose ChEBI	+	builds acid from
[Ref.: #23013]	dna ChEBI	-	hydrolysis
[Ref.: #23013]	esculin ChEBI	+	builds acid from
[Ref.: #23013]	esculin ChEBI	+	hydrolysis
[Ref.: #23013]	ethanol ChEBI	-	builds acid from
[Ref.: #23013]	gelatin ChEBI	-	hydrolysis
[Ref.: #23013]	gentiobiose ChEBI	-	builds acid from
[Ref.: #23013]	glycerol ChEBI	+	builds acid from
[Ref.: #23013]	L-arabinose ChEBI	-	builds acid from
[Ref.: #23013]	L-sorbose ChEBI	-	builds acid from
[Ref.: #23013]	lactose ChEBI	-	builds acid from
[Ref.: #23013]	maltose ChEBI	+	builds acid from
[Ref.: #23013]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23013]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #23013]	nitrate ChEBI	-	reduction
[Ref.: #23013]	raffinose ChEBI	-	builds acid from
[Ref.: #23013]	salicin ChEBI	+	builds acid from
[Ref.: #23013]	sorbitol ChEBI	-	builds acid from
[Ref.: #23013]	starch ChEBI	-	hydrolysis
[Ref.: #23013]	sucrose ChEBI	+	builds acid from
[Ref.: #23013]	trehalose ChEBI	+	builds acid from
[Ref.: #23013]	tween 40 ChEBI	+	hydrolysis
[Ref.: #23013]	tween 60 ChEBI	+	hydrolysis
[Ref.: #23013]	tween 80 ChEBI	-	hydrolysis
[Ref.: #23013]	tyrosine ChEBI	-	hydrolysis
[Ref.: #23013]	urea ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #23013]	amoxicillin ChEBI	yes	10	µg (disc)
[Ref.: #23013]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #23013]	carbenicillin ChEBI	yes	100	µg (disc)
[Ref.: #23013]	cefotaxime ChEBI	yes	30	µg (disc)
[Ref.: #23013]	cefoxitin ChEBI	no			yes	30	µg (disc)
[Ref.: #23013]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #23013]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #23013]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #23013]	neomycin ChEBI	yes	30	µg (disc)
[Ref.: #23013]	nitrofurantoin ChEBI	yes	300	µg (disc)
[Ref.: #23013]	novobiocin ChEBI	yes	30	µg (disc)
[Ref.: #23013]	nystatin ChEBI	no			yes	100	µg (disc)
[Ref.: #23013]	penicillin ChEBI	no			yes	10	µg (disc)
[Ref.: #23013]	polymyxin b ChEBI	no			yes	300	Unit
[Ref.: #23013]	rifampicin ChEBI	yes	5	µg (disc)
[Ref.: #23013]	streptomycin ChEBI	no			yes	10	µg (disc)
[Ref.: #23013]	tetracycline ChEBI	no			yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #23013]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #23013]	acid phosphatase	+	3.1.3.2
[Ref.: #23013]	alkaline phosphatase	+	3.1.3.1
[Ref.: #23013]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #23013]	alpha-fucosidase	-	3.2.1.51
[Ref.: #23013]	alpha-galactosidase	-	3.2.1.22
[Ref.: #23013]	alpha-glucosidase	+	3.2.1.20
[Ref.: #23013]	alpha-mannosidase	-	3.2.1.24
[Ref.: #23013]	arginine dihydrolase	-	3.5.3.6
[Ref.: #23013]	beta-galactosidase	+	3.2.1.23
[Ref.: #23013]	beta-galactosidase	-	3.2.1.23
[Ref.: #23013]	beta-glucosidase	+	3.2.1.21
[Ref.: #23013]	beta-glucuronidase	-	3.2.1.31
[Ref.: #23013]	catalase	+	1.11.1.6
[Ref.: #23013]	cystine arylamidase	-	3.4.11.3
[Ref.: #23013]	cytochrome oxidase	+	1.9.3.1
[Ref.: #23013]	esterase (C 4)	+
[Ref.: #23013]	esterase lipase (C 8)	+
[Ref.: #23013]	leucine arylamidase	-	3.4.11.1
[Ref.: #23013]	lipase (C 14)	-
[Ref.: #23013]	lysine decarboxylase	-	4.1.1.18
[Ref.: #23013]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #23013]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #23013]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #23013]	trypsin	-	3.4.21.4
[Ref.: #23013]	tryptophan deaminase	-	4.1.99.1
[Ref.: #23013]	urease	-	3.5.1.5
[Ref.: #23013]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17756]	Sample type/isolated from	activated sludge of a bioreactor, Szechuan pickle factory
[Ref.: #17756]	Country	China
[Ref.: #17756]	Country ISO 3 Code	CHN
[Ref.: #17756]	Continent	Asia

[Ref.: #23013]	Sample type/isolated from	activated sludge of a bioreactor treating salt-containing wastewater preserved Szechuan pickle factory
[Ref.: #23013]	Enrichment culture	sterile wastewater agar plates
[Ref.: #23013]	Enrichment culture composition	containing per litre wastewater: 1.0 g glucose, 1.0 g peptone (BD) and 15.0 g agar (pH 7.0)
[Ref.: #23013]	Enrichment culture temperature	30.0 ̊C

[Ref.: #67770]	Sample type/isolated from	Activated sludge of a bioreactor treating salt-containing wastewater
* marker position based on {}

Isolation sources categories

#Condition	#Saline	-
#Engineered	#Bioreactor	-
#Engineered	#Industrial	-
#Engineered	#Waste	#Activated sludge

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17756]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #17756]	Oceanobacillus neutriphilus strain A1g 16S ribosomal RNA gene, partial sequence	EU709018	1484	GenBank ENA	531815 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Oceanobacillus neutriphilus CGMCC 1.7693	GCA_014645515	contig	GenBank ENA	531815 tax ID	*
[Ref.: #66792]	Oceanobacillus neutriphilus strain CGMCC 1.7693	531815.3	wgs	PATRIC	531815 tax ID	*
[Ref.: #66792]	Oceanobacillus neutriphilus strain DSM 24772	531815.4	wgs	PATRIC	531815 tax ID	*
[Ref.: #66792]	Oceanobacillus neutriphilus DSM 24772	2901131393	draft	JGI IMG/M	531815 tax ID	*

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GC-content

36.3 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Oceanobacillus neutriphilus strain DSM 24772 PATRIC: 531815.4
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Oceanobacillus neutriphilus strain CGMCC 1.7693 PATRIC: 531815.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	79.611	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	94.561	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	92.186	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	57.728	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	93.207	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	84.035	yes

Availability in culture collections External links

[Ref.: #17756]

Culture collection no.

DSM 24772, CGMCC 1.7693, JCM 15776, BCRC 80658, KCTC 13886

[Ref.: #71031]

SI-ID 404977

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Oceanobacillus aidingensis sp. nov., a moderately halophilic bacterium.	Liu W, Yang SS	Antonie Van Leeuwenhoek	10.1007/s10482-014-0128-1	2014	*
Phylogeny	Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor.	Yang JY, Huo YY, Xu XW, Meng FX, Wu M, Wang CS	Int J Syst Evol Microbiol	10.1099/ijs.0.016295-0	2009	*

References

#17756

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24772

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23013

Jun-Yi Yang,Ying-Yi Huo,Xue-Wei Xu,Fan-Xu Meng,Min Wu,Chun-Sheng Wang: Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor. IJSEM 60: 2409 - 2414 2010 ( DOI 10.1099/ijs.0.016295-0 , PubMed 19946056 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71031

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404977.1 )

* These data were automatically processed and therefore are not curated

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Oceanobacillus neutriphilus strain DSM 24772

Oceanobacillus neutriphilus strain CGMCC 1.7693

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