Strain identifier

BacDive ID: 137804

Type strain: No

Species: Pseudomonas aeruginosa

Strain Designation: PA-103

Strain history: CIP <- 1987, ATCC <- P.V. Liu: strain PA-103 <- Louisville Gen. Hosp. USA

NCBI tax ID(s): 287 (species)

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General

@ref: 38746

BacDive-ID: 137804

keywords: Bacteria, aerobe, Gram-negative, motile, rod-shaped

description: Pseudomonas aeruginosa PA-103 is an aerobe, Gram-negative, motile bacterium of the family Pseudomonadaceae.

NCBI tax id

  • NCBI tax id: 287
  • Matching level: species

strain history

  • @ref: 38746
  • history: CIP <- 1987, ATCC <- P.V. Liu: strain PA-103 <- Louisville Gen. Hosp. USA

doi: 10.13145/bacdive137804.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas aeruginosa
  • full scientific name: Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacterium aeruginosum

@ref: 38746

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas aeruginosa

strain designation: PA-103

type strain: no

Morphology

cell morphology

  • @ref: 38746
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
38746MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
38746CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
38746positivegrowth30
50648positivegrowth37

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
50648aerobe
38746obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction
3874617632nitrate+reduction
3874616301nitrite+reduction

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
3874635581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase+3.5.3.6
38746oxidase+
38746catalase+1.11.1.6
38746urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
38746-+++-+--+-++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
50648+--+-+-++--++-+++++-+

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinentsample typesampling dategeographic location
38746USAUSANorth America
50648USAUSANorth AmericaHuman sputum1960Louisville
38746United States of AmericaUSANorth AmericaHuman, Sputum

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Fluids#Sputum

Safety information

risk assessment

  • @ref: 38746
  • biosafety level: 2
  • biosafety level comment: Risk group (French classification)

External links

@ref: 38746

culture collection no.: CIP 102967, ATCC 29260, CCUG 31589, NCIMB 11965

straininfo link

  • @ref: 94624
  • straininfo: 39442

literature

  • topic: Enzymology
  • Pubmed-ID: 1972559
  • title: Determination of genome size of Pseudomonas aeruginosa by PFGE: analysis of restriction fragments.
  • authors: Hector JS, Johnson AR
  • journal: Nucleic Acids Res
  • DOI: 10.1093/nar/18.11.3171
  • year: 1990
  • mesh: DNA Restriction Enzymes/metabolism, DNA, Bacterial/genetics, Electrophoresis, Polyacrylamide Gel, *Genes, Bacterial, *Polymorphism, Restriction Fragment Length, Pseudomonas aeruginosa/*genetics
  • topic2: Metabolism

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
38746Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102967Collection of Institut Pasteur (CIP 102967)
50648Curators of the CCUGhttps://www.ccug.se/strain?id=31589Culture Collection University of Gothenburg (CCUG) (CCUG 31589)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
94624Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID39442.1