Strain identifier

BacDive ID: 136730

Type strain: Yes

Species: Mycobacterium wolinskyi

Strain Designation: TBC 8878, M0739, Mo739

Strain history: CIP <- 2003, V. Vincent, Inst. Pasteur, Paris, France <- R.J. Wallace & B.A. Brown, Univ. of Texas Health Center, Texas, USA: strain Mo739 <- G.E. Pfyffer, Swiss Nat. Center Zürich, Switzerland

NCBI tax ID(s): 59750 (species)

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General

@ref: 64151

BacDive-ID: 136730

DSM-Number: 44493

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive

description: Mycobacterium wolinskyi TBC 8878 is a microaerophile, mesophilic, Gram-positive bacterium of the family Mycobacteriaceae.

NCBI tax id

  • NCBI tax id: 59750
  • Matching level: species

strain history

@refhistory
64151<- CIP <- G. E. Pfyffer; M0739
67770CCUG 47168 <-- ATCC 700010 <-- R. J. Wallace MO739 <-- G. E. Pfyffer TBC 8878.
123956CIP <- 2003, V. Vincent, Inst. Pasteur, Paris, France <- R.J. Wallace & B.A. Brown, Univ. of Texas Health Center, Texas, USA: strain Mo739 <- G.E. Pfyffer, Swiss Nat. Center Zürich, Switzerland

doi: 10.13145/bacdive136730.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium wolinskyi
  • full scientific name: Mycobacterium wolinskyi Brown et al. 1999

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium wolinskyi

@ref: 64151

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium wolinskyi

full scientific name: Mycobacterium wolinskyi Brown et al. 1999 emend. Nouioui et al. 2018

strain designation: TBC 8878, M0739, Mo739

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
123956rod-shapedno

colony morphology

  • @ref: 57551
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
64151MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
36249MEDIUM 55 - for Mycobacteriumyes
123956CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
64151positivegrowth37mesophilic
36249positivegrowth30mesophilic
67770positivegrowth37mesophilic
123956positivegrowth22-30
123956nogrowth10psychrophilic
123956nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
57551microaerophile
123956obligate aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.89

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
123956606565hippurate-hydrolysis
12395617632nitrate+reduction
12395616301nitrite-reduction
12395617632nitrate-respiration

antibiotic resistance

  • @ref: 123956
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 123956
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
123956oxidase-
123956beta-galactosidase-3.2.1.23
123956gelatinase-
123956amylase-
123956DNase-
123956caseinase-3.4.21.50
123956catalase+1.11.1.6
123956tween esterase-
123956gamma-glutamyltransferase+2.3.2.2
123956lecithinase-
123956lipase-
123956protease-
123956urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123956--++-+----++----+---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123956-+---------------------+--+++--+++--++--+----+---+-----+----+------+--+++--+---++---+--+++-+++--+-+

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinentsample type
36249SwitzerlandCHEEurope
57551SwitzerlandCHEEuropeHuman post-surgical facial abscess
64151SwitzerlandCHEEuropehuman facial wound
67770SwitzerlandCHEEuropePost-surgical facial abscess
123956SwitzerlandCHEEuropeFace

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Other#Abscess
#Host Body-Site#Other#Head
#Host#Human

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
641512Risk group (German classification)
1239561Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
64151Mycolicibacterium wolinskyi strain ATCC 700010 16S ribosomal RNA gene, partial sequenceAY4570831485ena59750
67770M.wolinsky 16S rRNA gene, strain ATCC 700010Y128731413ena59750

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium wolinskyi strain ATCC 70001059750.5wgspatric59750
67770Mycolicibacterium wolinskyi ATCC 700010GCA_002101965contigncbi59750
66792Mycolicibacterium wolinskyi CCUG 47168GCA_025822475scaffoldncbi59750

GC content

@refGC-contentmethod
6777066high performance liquid chromatography (HPLC)
6777066.4genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno81no
gram-positiveyes88.22no
anaerobicno99.376yes
halophileno88.173no
spore-formingno77.808no
glucose-utilyes90.489no
thermophileno99.604yes
flagellatedno97.873no
aerobicyes88.335yes
motileno91.584no
glucose-fermentno90.837no

External links

@ref: 64151

culture collection no.: DSM 44493, ATCC 700010, CIP 106348, CCUG 48179, CCUG 47168, JCM 13393

straininfo link

  • @ref: 93800
  • straininfo: 43724

literature

  • topic: Phylogeny
  • Pubmed-ID: 10555330
  • title: Mycobacterium wolinskyi sp. nov. and Mycobacterium goodii sp. nov., two new rapidly growing species related to Mycobacterium smegmatis and associated with human wound infections: a cooperative study from the International Working Group on Mycobacterial Taxonomy.
  • authors: Brown BA, Springer B, Steingrube VA, Wilson RW, Pfyffer GE, Garcia MJ, Menendez MC, Rodriguez-Salgado B, Jost KC Jr, Chiu SH, Onyi GO, Bottger EC, Wallace RJ Jr
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-49-4-1493
  • year: 1999
  • mesh: Adolescent, Adult, Aged, Aged, 80 and over, *Bacterial Proteins, Bacterial Typing Techniques, Base Composition, Base Sequence, Chaperonin 60, Chaperonins/genetics, Chromatography, High Pressure Liquid, DNA, Bacterial/chemistry/genetics, Female, Genes, rRNA/genetics, Humans, Male, Microbial Sensitivity Tests, Middle Aged, Molecular Sequence Data, Mycobacterium/*classification/genetics/isolation & purification/physiology, Mycobacterium Infections/*microbiology, Mycobacterium smegmatis/classification/genetics/isolation & purification, Nucleic Acid Hybridization, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Wound Infection/*microbiology
  • topic2: Pathogenicity

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
36249Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18469
57551Curators of the CCUGhttps://www.ccug.se/strain?id=47168Culture Collection University of Gothenburg (CCUG) (CCUG 47168)
64151Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-44493Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44493)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
93800Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID43724.1
123956Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106348Collection of Institut Pasteur (CIP 106348)