Strain identifier

BacDive ID: 1367

Type strain: Yes

Species: Lentibacillus jeotgali

Strain Designation: Grbi

Strain history: <- JW Bae, KRIBB

NCBI tax ID(s): 558169 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9 (current version):
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 9 (current version)

General

@ref: 17307

BacDive-ID: 1367

DSM-Number: 23924

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, Gram-positive, rod-shaped, colony-forming

description: Lentibacillus jeotgali Grbi is an obligate aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from traditional Korean fermented seafood made from scallops, "Garibi-jeotgal".

NCBI tax id

  • NCBI tax id: 558169
  • Matching level: species

strain history

@refhistory
17307<- KCTC <- J.-W. Bae, Korea Res. Inst. Biosci. & Biotechnol. (KRIBB), Taejon, South Korea
67770J.-W. Bae <-- M.-J. Jung strain Grbi.
67771<- JW Bae, KRIBB

doi: 10.13145/bacdive1367.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Lentibacillus
  • species: Lentibacillus jeotgali
  • full scientific name: Lentibacillus jeotgali Jung et al. 2010

@ref: 17307

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Lentibacillus

species: Lentibacillus jeotgali

full scientific name: Lentibacillus jeotgali Jung et al. 2010

strain designation: Grbi

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
23004positive2.0-4.0 µm0.5-1.0 µmrod-shapedno
67771rod-shapedno
67771positive

colony morphology

  • @ref: 23004
  • colony size: 1.0-2.0 mm
  • colony color: opaque ivory
  • colony shape: circular
  • incubation period: 3 days
  • medium used: MA supplemented with 10 % (w/v) NaCl

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17307BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 100.0 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
23004MA supplemented with 10 % (w/v) NaClyes

culture temp

@refgrowthtypetemperature
23004positivegrowth10.0-45.0
23004positiveoptimum37.0
67770positivegrowth37
67771positivegrowth37

culture pH

@refabilitytypepH
23004positivegrowth6.0-8.0
23004positiveoptimum8.0

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23004obligate aerobe
67771aerobe

spore formation

  • @ref: 23004
  • spore description: single,terminal,spherical,in swollen sporangia
  • type of spore: endospore
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentrationhalophily level
23004NaClpositivegrowth3.0-20.0 %(w/v)
23004NaCloptimum10.0-15.0 %
23004moderately halophilic

observation

@refobservation
67770quinones: MK-7
67771quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
23004174265-dehydro-D-gluconate-builds acid from
2300427613amygdalin-builds acid from
2300417057cellobiose-builds acid from
2300415963ribitol-builds acid from
2300417108D-arabinose-builds acid from
2300418333D-arabitol-builds acid from
2300428847D-fucose-builds acid from
2300417716lactose-builds acid from
2300462318D-lyxose-builds acid from
2300416024D-mannose-builds acid from
2300417924D-sorbitol-builds acid from
2300416443D-tagatose-builds acid from
2300465327D-xylose-builds acid from
2300416813galactitol-builds acid from
2300417113erythritol-builds acid from
2300428066gentiobiose-builds acid from
2300424265gluconate-builds acid from
2300417268myo-inositol-builds acid from
2300430849L-arabinose-builds acid from
2300418403L-arabitol-builds acid from
2300418287L-fucose-builds acid from
2300462345L-rhamnose-builds acid from
2300417266L-sorbose-builds acid from
2300465328L-xylose-builds acid from
230046731melezitose-builds acid from
2300428053melibiose-builds acid from
23004320055methyl beta-D-glucopyranoside-builds acid from
2300474863methyl beta-D-xylopyranoside-builds acid from
23004506227N-acetylglucosamine-builds acid from
2300416634raffinose-builds acid from
2300417814salicin-builds acid from
2300428017starch-builds acid from
2300417992sucrose-builds acid from
2300427082trehalose-builds acid from
2300432528turanose-builds acid from
2300417151xylitol-builds acid from
2300429016arginine-hydrolysis
23004casein-hydrolysis
2300462968cellulose-hydrolysis
2300416991dna-hydrolysis
230045291gelatin-hydrolysis
2300417368hypoxanthine-hydrolysis
2300428017starch-hydrolysis
2300453426tween 80-hydrolysis
2300418186tyrosine-hydrolysis
2300415318xanthine-hydrolysis
23004168082-dehydro-D-gluconate+builds acid from
2300418305arbutin+builds acid from
2300415824D-fructose+builds acid from
2300412936D-galactose+builds acid from
2300417634D-glucose+builds acid from
2300416899D-mannitol+builds acid from
2300416988D-ribose+builds acid from
230044853esculin+builds acid from
2300417754glycerol+builds acid from
2300428087glycogen+builds acid from
2300415443inulin+builds acid from
2300417306maltose+builds acid from
2300443943methyl alpha-D-mannoside+builds acid from
230044853esculin+hydrolysis

enzymes

@refvalueactivityec
23004catalase+1.11.1.6
23004cytochrome oxidase-1.9.3.1
23004urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentenrichment cultureenrichment culture temperature
17307traditional Korean fermented seafood made from scallops, "Garibi-jeotgal"Republic of KoreaKORAsia
23004marine 2216 agar (MA; BBL) plates supplemented with 20 % (w/v) NaCl30.0
67770Jeotgal, traditional Korean fermented seafoodRepublic of KoreaKORAsia
67771From Garibi-jeotgal, traditional Korean fermented seafood made from scallopsRepublic of KoreaKORAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Seafood
#Engineered#Food production#Fermented

taxonmaps

  • @ref: 69479
  • File name: preview.99_7345.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_3088;97_4217;98_5388;99_7345&stattab=map
  • Last taxonomy: Lentibacillus jeotgali
  • 16S sequence: FJ182050
  • Sequence Identity:
  • Total samples: 164
  • soil counts: 26
  • aquatic counts: 14
  • animal counts: 122
  • plant counts: 2

Safety information

risk assessment

  • @ref: 17307
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17307
  • description: Lentibacillus jeotgali strain Grbi 16S ribosomal RNA gene, partial sequence
  • accession: FJ182050
  • length: 1414
  • database: nuccore
  • NCBI tax ID: 558169

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lentibacillus jeotgali Grbi2548876534draftimg558169
67770Lentibacillus jeotgali GrbiGCA_000224785contigncbi558169

GC content

@refGC-contentmethod
1730742.5fluorimetric
6777042.5high performance liquid chromatography (HPLC)
6777142.5

External links

@ref: 17307

culture collection no.: DSM 23924, JCM 15795, KCTC 13300

straininfo link

  • @ref: 71018
  • straininfo: 403619

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19666788Lentibacillus jeotgali sp. nov., a halophilic bacterium isolated from traditional Korean fermented seafood.Jung MJ, Roh SW, Kim MS, Bae JWInt J Syst Evol Microbiol10.1099/ijs.0.013565-02009Animals, Bacillaceae/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Fermentation, Genes, rRNA, Genotype, Korea, Molecular Sequence Data, Nucleic Acid Hybridization, Pectinidae/*microbiology, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Seafood/*microbiology, Sequence Analysis, DNA, *Sodium Chloride, Species SpecificityGenetics
Phylogeny26545789Lentibacillus amyloliquefaciens sp. nov., a halophilic bacterium isolated from saline sediment sample.Wang JL, Ma KD, Wang YW, Wang HM, Li YB, Zhou S, Chen XR, Kong DL, Guo X, He MX, Ruan ZYAntonie Van Leeuwenhoek10.1007/s10482-015-0618-92015Bacillaceae/classification/genetics/*isolation & purification/metabolism, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Geologic Sediments/analysis/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sodium Chloride/analysis/*metabolismMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17307Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23924)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23924
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23004Mi-Ja Jung,Seong Woon Roh,Min-Soo Kim,Jin-Woo Bae10.1099/ijs.0.013565-0Lentibacillus jeotgali sp. nov., a halophilic bacterium isolated from traditional Korean fermented seafoodIJSEM 60: 1017-1022 201019666788
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71018Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID403619.1StrainInfo: A central database for resolving microbial strain identifiers